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The map label for this gene is lacR [H]

Identifier: 229916776

GI number: 229916776

Start: 996737

End: 997519

Strand: Reverse

Name: lacR [H]

Synonym: EAT1b_1049

Alternate gene names: 229916776

Gene position: 997519-996737 (Counterclockwise)

Preceding gene: 229916777

Following gene: 229916775

Centisome position: 33.25

GC content: 48.66

Gene sequence:

>783_bases
ATGTTGAAGGAACAGCGATGGGATCGCATCTTAGAACTGGTGGATACAAAGGGGGTCGTGAAGACGACCGAAGTGACGGA
ATTGCTTGGTGTCACGCCAATGACAGCACGTCGCGATTTGCAGATGCTTGAAGAATTAGGGCGACTTGAACGGATTCACG
GAGGTGCGAAATCCTTGAACATGGACCGTCCCCTGTCGTATGAAGAGTTGTCTCATGTCGAGAAGAAACAACTTCGTTCG
GAAGAGAAGCGGAGCATCGCCAGAAAAGCGGCGGCGCTTATTGAAGAGAATGCCACGGTGTTCATCGGACCGGGGACGAC
CAATGAACTCATTTATGAATACATCGACGTGAATGCCCGGATTTTTACGAATTCGTTCCAAGTGTTCGAGCGGTTTAAAA
ACGATGCGCGATTCGAATTGATGCTGATTGGTGGACGCTACCGCGAACGGACGGGTGCTTTTGTTGGAAGCTTTACAAAC
GATATCATGTCCCGCATCAACGTATCACTCGCTTTCATTGGAACGAACGGCGTGCACAATGAACGGATCACGACGGCCAA
CGAAGAAGAGGGCGCATGTCAAAAGATTATTCTCGATAATGCACAGTTGAAGTACATCTTGTGCGATTCAAGTAAAATCG
GTCGCGTCGATTTATTCGACTTCTACGGCTTGGATCACGTCGATGGACTGATTACGGACGAAAACATCAGCGAGGCGACG
TTACACCATTACTCCTCTTATACCGAGGTCATCGTGGGACAAAACGAACAGCAATTGGTTTGA

Upstream 100 bases:

>100_bases
GTTCTTTTGAACCGACTTTTGTTTGTTTCTGTTCTAAAATGATAGTCAAGGAGTGTTCAAGTTGAGTATACTGAAAGAAA
GAAGGAGGGTGATCGGGAAA

Downstream 100 bases:

>100_bases
CCATATGAATCAACGACCTGGATTGTTACGAAATGTGACAATCCAGGTCTTTTTTTGTTAAAAAACAAACAAATAATGTT
CGAAAATGATTGAAAATAAT

Product: DeoR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 260; Mature: 260

Protein sequence:

>260_residues
MLKEQRWDRILELVDTKGVVKTTEVTELLGVTPMTARRDLQMLEELGRLERIHGGAKSLNMDRPLSYEELSHVEKKQLRS
EEKRSIARKAAALIEENATVFIGPGTTNELIYEYIDVNARIFTNSFQVFERFKNDARFELMLIGGRYRERTGAFVGSFTN
DIMSRINVSLAFIGTNGVHNERITTANEEEGACQKIILDNAQLKYILCDSSKIGRVDLFDFYGLDHVDGLITDENISEAT
LHHYSSYTEVIVGQNEQQLV

Sequences:

>Translated_260_residues
MLKEQRWDRILELVDTKGVVKTTEVTELLGVTPMTARRDLQMLEELGRLERIHGGAKSLNMDRPLSYEELSHVEKKQLRS
EEKRSIARKAAALIEENATVFIGPGTTNELIYEYIDVNARIFTNSFQVFERFKNDARFELMLIGGRYRERTGAFVGSFTN
DIMSRINVSLAFIGTNGVHNERITTANEEEGACQKIILDNAQLKYILCDSSKIGRVDLFDFYGLDHVDGLITDENISEAT
LHHYSSYTEVIVGQNEQQLV
>Mature_260_residues
MLKEQRWDRILELVDTKGVVKTTEVTELLGVTPMTARRDLQMLEELGRLERIHGGAKSLNMDRPLSYEELSHVEKKQLRS
EEKRSIARKAAALIEENATVFIGPGTTNELIYEYIDVNARIFTNSFQVFERFKNDARFELMLIGGRYRERTGAFVGSFTN
DIMSRINVSLAFIGTNGVHNERITTANEEEGACQKIILDNAQLKYILCDSSKIGRVDLFDFYGLDHVDGLITDENISEAT
LHHYSSYTEVIVGQNEQQLV

Specific function: Repressor of the lactose catabolism operon. Galactose-6- phosphate is the inducer [H]

COG id: COG1349

COG function: function code KG; Transcriptional regulators of sugar metabolism

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH deoR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789829, Length=236, Percent_Identity=32.2033898305085, Blast_Score=112, Evalue=2e-26,
Organism=Escherichia coli, GI1787540, Length=243, Percent_Identity=30.8641975308642, Blast_Score=99, Evalue=3e-22,
Organism=Escherichia coli, GI226510968, Length=241, Percent_Identity=26.5560165975104, Blast_Score=99, Evalue=3e-22,
Organism=Escherichia coli, GI1789519, Length=242, Percent_Identity=28.9256198347107, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI1789059, Length=249, Percent_Identity=27.710843373494, Blast_Score=84, Evalue=8e-18,
Organism=Escherichia coli, GI87082344, Length=236, Percent_Identity=28.8135593220339, Blast_Score=82, Evalue=3e-17,
Organism=Escherichia coli, GI1790635, Length=244, Percent_Identity=26.6393442622951, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI1789170, Length=235, Percent_Identity=26.3829787234043, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI1788069, Length=238, Percent_Identity=26.0504201680672, Blast_Score=67, Evalue=8e-13,
Organism=Escherichia coli, GI1787063, Length=235, Percent_Identity=29.3617021276596, Blast_Score=67, Evalue=9e-13,

Paralogues:

None

Copy number: 900 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014036
- InterPro:   IPR001034
- InterPro:   IPR018356
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00455 DeoR; PF08220 HTH_DeoR [H]

EC number: NA

Molecular weight: Translated: 29613; Mature: 29613

Theoretical pI: Translated: 4.99; Mature: 4.99

Prosite motif: PS00894 HTH_DEOR_1 ; PS51000 HTH_DEOR_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKEQRWDRILELVDTKGVVKTTEVTELLGVTPMTARRDLQMLEELGRLERIHGGAKSLN
CCCHHHHHHHHHHHHCCCCEEHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCHHCCC
MDRPLSYEELSHVEKKQLRSEEKRSIARKAAALIEENATVFIGPGTTNELIYEYIDVNAR
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHCCCEE
IFTNSFQVFERFKNDARFELMLIGGRYRERTGAFVGSFTNDIMSRINVSLAFIGTNGVHN
EEHHHHHHHHHHCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHCEEEEEEECCCCCC
ERITTANEEEGACQKIILDNAQLKYILCDSSKIGRVDLFDFYGLDHVDGLITDENISEAT
CCCCCCCCCCCHHHHHEECCCEEEEEEECCCCCCCEEEHHHCCCHHHCCEEECCCCHHHH
LHHYSSYTEVIVGQNEQQLV
HHHHHHHHEEEECCCHHCCC
>Mature Secondary Structure
MLKEQRWDRILELVDTKGVVKTTEVTELLGVTPMTARRDLQMLEELGRLERIHGGAKSLN
CCCHHHHHHHHHHHHCCCCEEHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCHHCCC
MDRPLSYEELSHVEKKQLRSEEKRSIARKAAALIEENATVFIGPGTTNELIYEYIDVNAR
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHCCCEE
IFTNSFQVFERFKNDARFELMLIGGRYRERTGAFVGSFTNDIMSRINVSLAFIGTNGVHN
EEHHHHHHHHHHCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHCEEEEEEECCCCCC
ERITTANEEEGACQKIILDNAQLKYILCDSSKIGRVDLFDFYGLDHVDGLITDENISEAT
CCCCCCCCCCCHHHHHEECCCEEEEEEECCCCCCCEEEHHHCCCHHHCCEEECCCCHHHH
LHHYSSYTEVIVGQNEQQLV
HHHHHHHHEEEECCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2120234; 1901863 [H]