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The map label for this gene is lacG [H]

Identifier: 229916777

GI number: 229916777

Start: 997631

End: 999055

Strand: Reverse

Name: lacG [H]

Synonym: EAT1b_1050

Alternate gene names: 229916777

Gene position: 999055-997631 (Counterclockwise)

Preceding gene: 229916778

Following gene: 229916776

Centisome position: 33.3

GC content: 50.11

Gene sequence:

>1425_bases
ATGAGACAATTTCCGGAAGAGTTTATTTTTGGTGCGGCAACAGCAGCTTATCAAGCAGAAGGTGCGGTGAACGAAGGGGG
ACGCGGTCCTTGTTATTGGGATGAGTTCCTACATCGCCCGGAGAGCCGTTTCAATGGAGACGTCGCCAGTGATTTTTATC
ATCAGTATCCAGTCGATTTGAAGTTATCCAATGAGTATGGATTAAACGGCATTCGCATCTCGATTGCCTGGAGCCGTGTT
ATTCCGGATGGGGCCGGTGAGGTGAATGAGGAAGGACTCGCCTTTTATGACCGCTTGATCGATGAGTGTTTGGCAAACGG
TGTCGAGCCGGTCGTCACGCTTCATCATTTCGATACGCCGCTCCCGCTATTCCATCAAGGGGACTGGTTGAATCGTGACA
ACATTGACCACTTCGTCCGCTTTGCGGAAGTGTGCTTCGAGCGTTTCGGAGATCGCGTGAAGAAGTGGGTGTCAATCAAC
GAGCCATGGTCCGTCGTCGCGGGACAATACATCATCGGGCACTTCCCGCCAAACATTCGTTACGATGTCGCCAAAGCGAT
TCAAGCCATGCACCATATGATGATTGCTCATGCGAAAGTCGTGGACGCGTATAAACGGATGGAGCTAGACGGAGAGGTTG
GGATCATCCATATCCTCGAATCGAAATATCCGATCACCGAGTCCGAAGAAGACCGGGAAGCGGCATATGTGGAAGATGTG
TTGGCGAACCAATTCATGTTGGATGCCTGTTTGAAAGGAAGTTACACAGCCGAGACACTGACGGTCATCGAACGATTGAT
GTCTCTTCAACAAGCTACTCTTGTAGTCGAAGAGGGAGACATGGAATGGTTAGCGCGTGGCAGTGCCCAAAATGACTTCC
TTGGGGTCAACTATTATGCGAGTCATTTCTTGGCTGCCCATGATGGCGAGAGTGGGATTCATCATAACGGGACAGGGGAG
AAAGGGACGAGCGTGTTTGGCTTAAAAGGGATCGGGAAGCGCGTGTCGAATCCGGAAGTGCCTACAACGGATTGGGATTG
GCCAATTTATCCGACCGGCTTGACGGATATGCTCGTCCGGGTCCATCGTCAATATCCGAACTACAAAGCCATGTATGTGA
CGGAGAATGGAATGGGCTATAAGGACGACCTCGTCGACGGCTCGATCGATGACACGCCTCGAATTGATTATATCGCCCAG
CATTTGAACGCTATTTTGGATGCGATTGAACAAAGTGTCACGGTTCGTGGATATTTCCTCTGGTCTCTCATGGACGTCTT
GTCCTGGACGAATGGATTCAATAAACGGTATGGCTTGTTCTATGTGGATTTCGAGACACAGGAACGACTACCGAAGAAAA
GTGCACGTTGGATGAGAGAAGTGGCGAACCGACGTCAAGTCATTCAACCGGAAACTGTAAAATGA

Upstream 100 bases:

>100_bases
GGGGCACTCGCCTTCGCGATGAAGCATATGTAATACAAAAGGGGCTGGTCATTCACAGCCCCTCTAATCTTTTCAGTCTA
GACAAAGAGGTGGAATCGAA

Downstream 100 bases:

>100_bases
ACAAAAAGTCGGTTCTTTTGAACCGACTTTTGTTTGTTTCTGTTCTAAAATGATAGTCAAGGAGTGTTCAAGTTGAGTAT
ACTGAAAGAAAGAAGGAGGG

Product: 6-phospho-beta-galactosidase

Products: NA

Alternate protein names: Beta-D-phosphogalactoside galactohydrolase; PGALase; P-beta-Gal; PBG [H]

Number of amino acids: Translated: 474; Mature: 474

Protein sequence:

>474_residues
MRQFPEEFIFGAATAAYQAEGAVNEGGRGPCYWDEFLHRPESRFNGDVASDFYHQYPVDLKLSNEYGLNGIRISIAWSRV
IPDGAGEVNEEGLAFYDRLIDECLANGVEPVVTLHHFDTPLPLFHQGDWLNRDNIDHFVRFAEVCFERFGDRVKKWVSIN
EPWSVVAGQYIIGHFPPNIRYDVAKAIQAMHHMMIAHAKVVDAYKRMELDGEVGIIHILESKYPITESEEDREAAYVEDV
LANQFMLDACLKGSYTAETLTVIERLMSLQQATLVVEEGDMEWLARGSAQNDFLGVNYYASHFLAAHDGESGIHHNGTGE
KGTSVFGLKGIGKRVSNPEVPTTDWDWPIYPTGLTDMLVRVHRQYPNYKAMYVTENGMGYKDDLVDGSIDDTPRIDYIAQ
HLNAILDAIEQSVTVRGYFLWSLMDVLSWTNGFNKRYGLFYVDFETQERLPKKSARWMREVANRRQVIQPETVK

Sequences:

>Translated_474_residues
MRQFPEEFIFGAATAAYQAEGAVNEGGRGPCYWDEFLHRPESRFNGDVASDFYHQYPVDLKLSNEYGLNGIRISIAWSRV
IPDGAGEVNEEGLAFYDRLIDECLANGVEPVVTLHHFDTPLPLFHQGDWLNRDNIDHFVRFAEVCFERFGDRVKKWVSIN
EPWSVVAGQYIIGHFPPNIRYDVAKAIQAMHHMMIAHAKVVDAYKRMELDGEVGIIHILESKYPITESEEDREAAYVEDV
LANQFMLDACLKGSYTAETLTVIERLMSLQQATLVVEEGDMEWLARGSAQNDFLGVNYYASHFLAAHDGESGIHHNGTGE
KGTSVFGLKGIGKRVSNPEVPTTDWDWPIYPTGLTDMLVRVHRQYPNYKAMYVTENGMGYKDDLVDGSIDDTPRIDYIAQ
HLNAILDAIEQSVTVRGYFLWSLMDVLSWTNGFNKRYGLFYVDFETQERLPKKSARWMREVANRRQVIQPETVK
>Mature_474_residues
MRQFPEEFIFGAATAAYQAEGAVNEGGRGPCYWDEFLHRPESRFNGDVASDFYHQYPVDLKLSNEYGLNGIRISIAWSRV
IPDGAGEVNEEGLAFYDRLIDECLANGVEPVVTLHHFDTPLPLFHQGDWLNRDNIDHFVRFAEVCFERFGDRVKKWVSIN
EPWSVVAGQYIIGHFPPNIRYDVAKAIQAMHHMMIAHAKVVDAYKRMELDGEVGIIHILESKYPITESEEDREAAYVEDV
LANQFMLDACLKGSYTAETLTVIERLMSLQQATLVVEEGDMEWLARGSAQNDFLGVNYYASHFLAAHDGESGIHHNGTGE
KGTSVFGLKGIGKRVSNPEVPTTDWDWPIYPTGLTDMLVRVHRQYPNYKAMYVTENGMGYKDDLVDGSIDDTPRIDYIAQ
HLNAILDAIEQSVTVRGYFLWSLMDVLSWTNGFNKRYGLFYVDFETQERLPKKSARWMREVANRRQVIQPETVK

Specific function: Unknown

COG id: COG2723

COG function: function code G; Beta-glucosidase/6-phospho-beta-glucosidase/beta-galactosidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 1 family [H]

Homologues:

Organism=Homo sapiens, GI13273313, Length=475, Percent_Identity=33.0526315789474, Blast_Score=247, Evalue=2e-65,
Organism=Homo sapiens, GI32481206, Length=487, Percent_Identity=32.2381930184805, Blast_Score=218, Evalue=1e-56,
Organism=Homo sapiens, GI110681710, Length=501, Percent_Identity=31.3373253493014, Blast_Score=214, Evalue=1e-55,
Organism=Homo sapiens, GI28376633, Length=475, Percent_Identity=29.0526315789474, Blast_Score=165, Evalue=1e-40,
Organism=Homo sapiens, GI24497614, Length=488, Percent_Identity=28.2786885245902, Blast_Score=144, Evalue=2e-34,
Organism=Homo sapiens, GI190360571, Length=95, Percent_Identity=43.1578947368421, Blast_Score=79, Evalue=7e-15,
Organism=Escherichia coli, GI2367174, Length=493, Percent_Identity=33.4685598377282, Blast_Score=243, Evalue=2e-65,
Organism=Escherichia coli, GI2367270, Length=490, Percent_Identity=34.4897959183673, Blast_Score=232, Evalue=4e-62,
Organism=Escherichia coli, GI1789070, Length=495, Percent_Identity=32.3232323232323, Blast_Score=224, Evalue=7e-60,
Organism=Caenorhabditis elegans, GI17552856, Length=479, Percent_Identity=34.8643006263048, Blast_Score=230, Evalue=1e-60,
Organism=Caenorhabditis elegans, GI17539390, Length=491, Percent_Identity=31.7718940936864, Blast_Score=218, Evalue=8e-57,
Organism=Drosophila melanogaster, GI21356577, Length=481, Percent_Identity=33.0561330561331, Blast_Score=244, Evalue=9e-65,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001360
- InterPro:   IPR018120
- InterPro:   IPR017853
- InterPro:   IPR013781
- InterPro:   IPR005928 [H]

Pfam domain/function: PF00232 Glyco_hydro_1 [H]

EC number: =3.2.1.85 [H]

Molecular weight: Translated: 54057; Mature: 54057

Theoretical pI: Translated: 4.83; Mature: 4.83

Prosite motif: PS00572 GLYCOSYL_HYDROL_F1_1 ; PS00653 GLYCOSYL_HYDROL_F1_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRQFPEEFIFGAATAAYQAEGAVNEGGRGPCYWDEFLHRPESRFNGDVASDFYHQYPVDL
CCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHCCCHHHCCCCHHHHHHHHCCCEE
KLSNEYGLNGIRISIAWSRVIPDGAGEVNEEGLAFYDRLIDECLANGVEPVVTLHHFDTP
EECCCCCCCEEEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEECCCC
LPLFHQGDWLNRDNIDHFVRFAEVCFERFGDRVKKWVSINEPWSVVAGQYIIGHFPPNIR
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCEEECCCCCCH
YDVAKAIQAMHHMMIAHAKVVDAYKRMELDGEVGIIHILESKYPITESEEDREAAYVEDV
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCHHHHHHHHHH
LANQFMLDACLKGSYTAETLTVIERLMSLQQATLVVEEGDMEWLARGSAQNDFLGVNYYA
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHEEEEECCCHHHHHCCCCCCCEEEEHHHH
SHFLAAHDGESGIHHNGTGEKGTSVFGLKGIGKRVSNPEVPTTDWDWPIYPTGLTDMLVR
HHHHEECCCCCCCCCCCCCCCCCEEEEEHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHH
VHRQYPNYKAMYVTENGMGYKDDLVDGSIDDTPRIDYIAQHLNAILDAIEQSVTVRGYFL
HHHHCCCCEEEEEECCCCCCHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
WSLMDVLSWTNGFNKRYGLFYVDFETQERLPKKSARWMREVANRRQVIQPETVK
HHHHHHHHHHCCCCCCCCEEEEEECHHHHCCHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MRQFPEEFIFGAATAAYQAEGAVNEGGRGPCYWDEFLHRPESRFNGDVASDFYHQYPVDL
CCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHCCCHHHCCCCHHHHHHHHCCCEE
KLSNEYGLNGIRISIAWSRVIPDGAGEVNEEGLAFYDRLIDECLANGVEPVVTLHHFDTP
EECCCCCCCEEEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEECCCC
LPLFHQGDWLNRDNIDHFVRFAEVCFERFGDRVKKWVSINEPWSVVAGQYIIGHFPPNIR
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCEEECCCCCCH
YDVAKAIQAMHHMMIAHAKVVDAYKRMELDGEVGIIHILESKYPITESEEDREAAYVEDV
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCHHHHHHHHHH
LANQFMLDACLKGSYTAETLTVIERLMSLQQATLVVEEGDMEWLARGSAQNDFLGVNYYA
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHEEEEECCCHHHHHCCCCCCCEEEEHHHH
SHFLAAHDGESGIHHNGTGEKGTSVFGLKGIGKRVSNPEVPTTDWDWPIYPTGLTDMLVR
HHHHEECCCCCCCCCCCCCCCCCEEEEEHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHH
VHRQYPNYKAMYVTENGMGYKDDLVDGSIDDTPRIDYIAQHLNAILDAIEQSVTVRGYFL
HHHHCCCCEEEEEECCCCCCHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
WSLMDVLSWTNGFNKRYGLFYVDFETQERLPKKSARWMREVANRRQVIQPETVK
HHHHHHHHHHCCCCCCCCEEEEEECHHHHCCHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11466286 [H]