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The map label for this gene is citZ [H]

Identifier: 229916397

GI number: 229916397

Start: 619433

End: 620545

Strand: Reverse

Name: citZ [H]

Synonym: EAT1b_0667

Alternate gene names: 229916397

Gene position: 620545-619433 (Counterclockwise)

Preceding gene: 229916398

Following gene: 229916396

Centisome position: 20.69

GC content: 47.89

Gene sequence:

>1113_bases
ATGTCAACGACAAAAGGGTTAGAAGGTATTGTGGCAGCAGCCTCAAAAGTCAGCTCAATTATTGACGGACAATTGACTTA
TGGAGGATACACCATTGATGATTTAGCGGACAACGCCACATTCGAAGAAGTAGTGTTCTTATTGTGGAACGACCGCTTAC
CAAATGCAGAGGAATTGACAGCGCTCTCAGAAGCGCTTGTAAAAGAAGCGACATTACCTGAAGAGGTGATTGATACGTTA
AAACGTGCGCCGAAGTCAGCCAATGCGATGGCAACGATTCGTACAGCACTCTCACAACTCGCTCTATACGATGAGGAATC
TGAAGACATGAGTGACGAGGCCAACATGCGTAAAGCAATTCGCCTACAAGCACAAGTCGCTACAATCGTGACGGCATTCG
CCCGTCTTCGTAAAGGGGAGCAGCCGGTCGCACCAAAACCTGGTCTTTCTTACGCTGCGAACTTCTTATACATGCTAAAC
GGTGAAGAGCCGAGCGATGTAGCAGAAAAGACGATTGATAAAGCGCTCATCTTACACGCAGACCACGAGTTGAATGCTTC
GACATTCACTGCTCGCGTGTGTGTAGCGACGCTTTCAGATATGTACTCAGGTGTCACGGCGGCTATGGGGGCACTCAAAG
GGCCACTTCACGGTGGTGCGAACGAAGCGGTAATGAAAATGTTGCACGAGATCGATTCGGTCGACCGTGTTGAAGAATAT
GTTCGTGGCAAATTCGAGCGTAAAGAGAAAATCATGGGCTTCGGTCACCGCGTTTACAAGGACGGCGACCCACGTGCGAA
GCATTTGAAAGAAATGAGCCGCCAATTGACTGCTCAAATCGGCGAACCGAAATGGTATGAGATGTCTGAGAAAATCGACC
ATCTTGTCGAAACGGAAAAAGGATTGAAACCGAATGTCGATTTCTATTCAGCTTCAACATATTATGCGCTCGGACTCGAG
GAAGATCTATTCACACCAATCTTCGCCGTATCTCGTATGTCTGGCTGGATTGCGCACATTCTTGAGCAATATGCGGACAA
TCGCTTGATTCGTCCACGTGCGGAATATGTCGGTGAGACGAGCCGTAAATATGTTCCGGTAAACGAACGTTAA

Upstream 100 bases:

>100_bases
GGGTGAATGCAGGGGAACAACCCGTCCCGTATCCTTATTCCCTATAAACAGACTGTTTTTACGGTCGACCACTTTAAACA
AGACTGAGGGGGAGTTATGA

Downstream 100 bases:

>100_bases
TTCGAGACACATTTTAGACAAAAGTTTGGTAAACTAACAAGTGACGTAGGTCGGCGCACGCGTCGACCTACAGTAATGTA
CTTAGGGGGATTCATTATGT

Product: 2-methylcitrate synthase/citrate synthase II

Products: NA

Alternate protein names: Citrate synthase II [H]

Number of amino acids: Translated: 370; Mature: 369

Protein sequence:

>370_residues
MSTTKGLEGIVAAASKVSSIIDGQLTYGGYTIDDLADNATFEEVVFLLWNDRLPNAEELTALSEALVKEATLPEEVIDTL
KRAPKSANAMATIRTALSQLALYDEESEDMSDEANMRKAIRLQAQVATIVTAFARLRKGEQPVAPKPGLSYAANFLYMLN
GEEPSDVAEKTIDKALILHADHELNASTFTARVCVATLSDMYSGVTAAMGALKGPLHGGANEAVMKMLHEIDSVDRVEEY
VRGKFERKEKIMGFGHRVYKDGDPRAKHLKEMSRQLTAQIGEPKWYEMSEKIDHLVETEKGLKPNVDFYSASTYYALGLE
EDLFTPIFAVSRMSGWIAHILEQYADNRLIRPRAEYVGETSRKYVPVNER

Sequences:

>Translated_370_residues
MSTTKGLEGIVAAASKVSSIIDGQLTYGGYTIDDLADNATFEEVVFLLWNDRLPNAEELTALSEALVKEATLPEEVIDTL
KRAPKSANAMATIRTALSQLALYDEESEDMSDEANMRKAIRLQAQVATIVTAFARLRKGEQPVAPKPGLSYAANFLYMLN
GEEPSDVAEKTIDKALILHADHELNASTFTARVCVATLSDMYSGVTAAMGALKGPLHGGANEAVMKMLHEIDSVDRVEEY
VRGKFERKEKIMGFGHRVYKDGDPRAKHLKEMSRQLTAQIGEPKWYEMSEKIDHLVETEKGLKPNVDFYSASTYYALGLE
EDLFTPIFAVSRMSGWIAHILEQYADNRLIRPRAEYVGETSRKYVPVNER
>Mature_369_residues
STTKGLEGIVAAASKVSSIIDGQLTYGGYTIDDLADNATFEEVVFLLWNDRLPNAEELTALSEALVKEATLPEEVIDTLK
RAPKSANAMATIRTALSQLALYDEESEDMSDEANMRKAIRLQAQVATIVTAFARLRKGEQPVAPKPGLSYAANFLYMLNG
EEPSDVAEKTIDKALILHADHELNASTFTARVCVATLSDMYSGVTAAMGALKGPLHGGANEAVMKMLHEIDSVDRVEEYV
RGKFERKEKIMGFGHRVYKDGDPRAKHLKEMSRQLTAQIGEPKWYEMSEKIDHLVETEKGLKPNVDFYSASTYYALGLEE
DLFTPIFAVSRMSGWIAHILEQYADNRLIRPRAEYVGETSRKYVPVNER

Specific function: Might regulate the synthesis and function of enzymes involved in later enzymatic steps of Krebs cycle. Loss in activity results in sporulation defect [H]

COG id: COG0372

COG function: function code C; Citrate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the citrate synthase family [H]

Homologues:

Organism=Homo sapiens, GI38327625, Length=376, Percent_Identity=26.3297872340426, Blast_Score=93, Evalue=4e-19,
Organism=Escherichia coli, GI1786527, Length=367, Percent_Identity=36.5122615803815, Blast_Score=247, Evalue=7e-67,
Organism=Escherichia coli, GI1786939, Length=377, Percent_Identity=33.6870026525199, Blast_Score=211, Evalue=7e-56,
Organism=Caenorhabditis elegans, GI17555174, Length=325, Percent_Identity=26.4615384615385, Blast_Score=94, Evalue=1e-19,
Organism=Saccharomyces cerevisiae, GI6319850, Length=394, Percent_Identity=25.3807106598985, Blast_Score=88, Evalue=3e-18,
Organism=Saccharomyces cerevisiae, GI6324328, Length=348, Percent_Identity=25.2873563218391, Blast_Score=86, Evalue=9e-18,
Organism=Saccharomyces cerevisiae, GI6325257, Length=215, Percent_Identity=28.8372093023256, Blast_Score=71, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24640124, Length=374, Percent_Identity=26.7379679144385, Blast_Score=100, Evalue=1e-21,
Organism=Drosophila melanogaster, GI24640126, Length=374, Percent_Identity=26.7379679144385, Blast_Score=100, Evalue=1e-21,
Organism=Drosophila melanogaster, GI21356863, Length=393, Percent_Identity=24.1730279898219, Blast_Score=79, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011278
- InterPro:   IPR016142
- InterPro:   IPR016143
- InterPro:   IPR002020
- InterPro:   IPR016141
- InterPro:   IPR019810 [H]

Pfam domain/function: PF00285 Citrate_synt [H]

EC number: =2.3.3.1 [H]

Molecular weight: Translated: 41121; Mature: 40990

Theoretical pI: Translated: 4.95; Mature: 4.95

Prosite motif: PS00480 CITRATE_SYNTHASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTTKGLEGIVAAASKVSSIIDGQLTYGGYTIDDLADNATFEEVVFLLWNDRLPNAEELT
CCCCHHHHHHHHHHHHHHHHHCCCEEECCEEHHHHHCCCCHHHHHHHHHCCCCCCHHHHH
ALSEALVKEATLPEEVIDTLKRAPKSANAMATIRTALSQLALYDEESEDMSDEANMRKAI
HHHHHHHHHCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHH
RLQAQVATIVTAFARLRKGEQPVAPKPGLSYAANFLYMLNGEEPSDVAEKTIDKALILHA
HHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHEEEEECCCCHHHHHHHHHHHHHEEEC
DHELNASTFTARVCVATLSDMYSGVTAAMGALKGPLHGGANEAVMKMLHEIDSVDRVEEY
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHH
VRGKFERKEKIMGFGHRVYKDGDPRAKHLKEMSRQLTAQIGEPKWYEMSEKIDHLVETEK
HHHHHHHHHHHHHCCHHHCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHC
GLKPNVDFYSASTYYALGLEEDLFTPIFAVSRMSGWIAHILEQYADNRLIRPRAEYVGET
CCCCCCCEEECCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCC
SRKYVPVNER
CCCCCCCCCC
>Mature Secondary Structure 
STTKGLEGIVAAASKVSSIIDGQLTYGGYTIDDLADNATFEEVVFLLWNDRLPNAEELT
CCCHHHHHHHHHHHHHHHHHCCCEEECCEEHHHHHCCCCHHHHHHHHHCCCCCCHHHHH
ALSEALVKEATLPEEVIDTLKRAPKSANAMATIRTALSQLALYDEESEDMSDEANMRKAI
HHHHHHHHHCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHH
RLQAQVATIVTAFARLRKGEQPVAPKPGLSYAANFLYMLNGEEPSDVAEKTIDKALILHA
HHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHEEEEECCCCHHHHHHHHHHHHHEEEC
DHELNASTFTARVCVATLSDMYSGVTAAMGALKGPLHGGANEAVMKMLHEIDSVDRVEEY
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHH
VRGKFERKEKIMGFGHRVYKDGDPRAKHLKEMSRQLTAQIGEPKWYEMSEKIDHLVETEK
HHHHHHHHHHHHHCCHHHCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHC
GLKPNVDFYSASTYYALGLEEDLFTPIFAVSRMSGWIAHILEQYADNRLIRPRAEYVGET
CCCCCCCEEECCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCC
SRKYVPVNER
CCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8045898; 9387221; 9384377 [H]