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The map label for this gene is mdh [H]

Identifier: 229916395

GI number: 229916395

Start: 617111

End: 618043

Strand: Reverse

Name: mdh [H]

Synonym: EAT1b_0665

Alternate gene names: 229916395

Gene position: 618043-617111 (Counterclockwise)

Preceding gene: 229916396

Following gene: 229916394

Centisome position: 20.6

GC content: 52.52

Gene sequence:

>933_bases
ATGATTCGTCGTAACAAAATTTCTGTTATTGGAAGTGGCTTCACCGGTGCAACGACTGCACTGTACTTGGCTCAACGTGA
GCTAGGGGATGTCGTACTGCTCGATATGGAAGAAAAAGTAGGACCGACGAAAGGGAAAGCGCTCGACATGCAAGAAACGG
CTCCGATTCAAGGGTTTGATTCGTGGGTCACAGGTACGTCTGATTACGCGGACACGGCCGATTCCGATGTGGTTGTCATT
ACCGCGGGAATCGCGCGCAAGCCGGGGATGAGCCGGGACGACCTCGTCGCAACGAACGCAAACGTCATGCGCTCGGTTGT
GAAAGAGATTGCCCGTTACTCTCCAGACGCCATCCTGATCGTATTGACGAACCCTGTAGACGCCATGACGTACGCGGCGT
TCAAAGAATCAGGGTTCCCGAAAGAACGCGTCATCGGTCAATCCGGTGTGTTGGATACGGCTCGTTTCCGAACGTTTGTG
GCACAAGAGCTGAATGTGTCCGTCAAAGACGTATCCGGATTCGTTCTTGGAGGTCATGGCGACGATATGGTGCCTCTCGT
ACGCTATTCGTACGCCGGAGGCATCCCGCTCGAGAAACTCATCCCACATGAGCGAATCGAGCAAATCGTCGAACGAACAC
GAAAAGGTGGCGGCGAGATTGTCCAACTATTGGGCGATGGCTCGGCGTATTATGCGCCAGCCGCTGCCATCGTGGAGATG
GTCGAAGCGATTGTAAAAGATCAGCGTCGAATCTTACCGGCCATCGCATACCTTGAGGGTGAGTACGGATTCCAAGACTT
GTATCTTGGTGTCCCGACGATTCTTGGGAAACATGGAATTGAGCAAGTGTTAGAGCTCGACTTGACGGATGAAGAAAAAG
CAGCACTATCGAAGTCTGCCGATTCTGTCCGCTCGGTATTAAGCGTCTTATAA

Upstream 100 bases:

>100_bases
CGACTTCGCACGTCTCATGGACGGCGCGACAGAAGTGAAATGTTCAGAGTTCGCGGATGAATTGATTAAAAACATGTAAG
ACGCCCGAGGAGGAGAGCTC

Downstream 100 bases:

>100_bases
GACTGAGGTGCTTCCTGTCATGTTACAGGAAGCACCTTTTTTGCGCCTTCTCTCTTATCGAACATGCTACACTGAAAGTG
GATTTGTTTTGTTTTTGTAA

Product: malate dehydrogenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 310; Mature: 310

Protein sequence:

>310_residues
MIRRNKISVIGSGFTGATTALYLAQRELGDVVLLDMEEKVGPTKGKALDMQETAPIQGFDSWVTGTSDYADTADSDVVVI
TAGIARKPGMSRDDLVATNANVMRSVVKEIARYSPDAILIVLTNPVDAMTYAAFKESGFPKERVIGQSGVLDTARFRTFV
AQELNVSVKDVSGFVLGGHGDDMVPLVRYSYAGGIPLEKLIPHERIEQIVERTRKGGGEIVQLLGDGSAYYAPAAAIVEM
VEAIVKDQRRILPAIAYLEGEYGFQDLYLGVPTILGKHGIEQVLELDLTDEEKAALSKSADSVRSVLSVL

Sequences:

>Translated_310_residues
MIRRNKISVIGSGFTGATTALYLAQRELGDVVLLDMEEKVGPTKGKALDMQETAPIQGFDSWVTGTSDYADTADSDVVVI
TAGIARKPGMSRDDLVATNANVMRSVVKEIARYSPDAILIVLTNPVDAMTYAAFKESGFPKERVIGQSGVLDTARFRTFV
AQELNVSVKDVSGFVLGGHGDDMVPLVRYSYAGGIPLEKLIPHERIEQIVERTRKGGGEIVQLLGDGSAYYAPAAAIVEM
VEAIVKDQRRILPAIAYLEGEYGFQDLYLGVPTILGKHGIEQVLELDLTDEEKAALSKSADSVRSVLSVL
>Mature_310_residues
MIRRNKISVIGSGFTGATTALYLAQRELGDVVLLDMEEKVGPTKGKALDMQETAPIQGFDSWVTGTSDYADTADSDVVVI
TAGIARKPGMSRDDLVATNANVMRSVVKEIARYSPDAILIVLTNPVDAMTYAAFKESGFPKERVIGQSGVLDTARFRTFV
AQELNVSVKDVSGFVLGGHGDDMVPLVRYSYAGGIPLEKLIPHERIEQIVERTRKGGGEIVQLLGDGSAYYAPAAAIVEM
VEAIVKDQRRILPAIAYLEGEYGFQDLYLGVPTILGKHGIEQVLELDLTDEEKAALSKSADSVRSVLSVL

Specific function: Catalyzes the reversible oxidation of malate to oxaloacetate [H]

COG id: COG0039

COG function: function code C; Malate/lactate dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the LDH/MDH superfamily. MDH type 3 family [H]

Homologues:

Organism=Homo sapiens, GI47059044, Length=314, Percent_Identity=37.2611464968153, Blast_Score=218, Evalue=6e-57,
Organism=Homo sapiens, GI221136809, Length=314, Percent_Identity=37.2611464968153, Blast_Score=218, Evalue=6e-57,
Organism=Homo sapiens, GI5031857, Length=313, Percent_Identity=36.1022364217252, Blast_Score=199, Evalue=4e-51,
Organism=Homo sapiens, GI260099723, Length=313, Percent_Identity=36.1022364217252, Blast_Score=198, Evalue=5e-51,
Organism=Homo sapiens, GI15082234, Length=314, Percent_Identity=33.7579617834395, Blast_Score=197, Evalue=1e-50,
Organism=Homo sapiens, GI291575128, Length=315, Percent_Identity=33.6507936507937, Blast_Score=188, Evalue=5e-48,
Organism=Homo sapiens, GI4557032, Length=315, Percent_Identity=33.6507936507937, Blast_Score=188, Evalue=5e-48,
Organism=Homo sapiens, GI9257228, Length=311, Percent_Identity=32.475884244373, Blast_Score=183, Evalue=1e-46,
Organism=Homo sapiens, GI4504973, Length=311, Percent_Identity=32.475884244373, Blast_Score=183, Evalue=1e-46,
Organism=Homo sapiens, GI260099727, Length=222, Percent_Identity=37.8378378378378, Blast_Score=144, Evalue=1e-34,
Organism=Homo sapiens, GI260099725, Length=201, Percent_Identity=39.3034825870647, Blast_Score=143, Evalue=2e-34,
Organism=Homo sapiens, GI207028494, Length=194, Percent_Identity=36.0824742268041, Blast_Score=128, Evalue=6e-30,
Organism=Homo sapiens, GI103472011, Length=305, Percent_Identity=28.5245901639344, Blast_Score=114, Evalue=9e-26,
Organism=Homo sapiens, GI21735621, Length=328, Percent_Identity=28.9634146341463, Blast_Score=102, Evalue=4e-22,
Organism=Homo sapiens, GI103472015, Length=209, Percent_Identity=30.1435406698565, Blast_Score=85, Evalue=7e-17,
Organism=Escherichia coli, GI1789632, Length=314, Percent_Identity=29.6178343949045, Blast_Score=96, Evalue=4e-21,
Organism=Caenorhabditis elegans, GI17535107, Length=272, Percent_Identity=30.5147058823529, Blast_Score=149, Evalue=2e-36,
Organism=Caenorhabditis elegans, GI17554310, Length=225, Percent_Identity=32.8888888888889, Blast_Score=96, Evalue=2e-20,
Organism=Saccharomyces cerevisiae, GI6322765, Length=293, Percent_Identity=27.6450511945392, Blast_Score=91, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6320125, Length=211, Percent_Identity=30.3317535545024, Blast_Score=79, Evalue=6e-16,
Organism=Saccharomyces cerevisiae, GI6324446, Length=205, Percent_Identity=29.2682926829268, Blast_Score=65, Evalue=9e-12,
Organism=Drosophila melanogaster, GI17136226, Length=306, Percent_Identity=31.0457516339869, Blast_Score=164, Evalue=7e-41,
Organism=Drosophila melanogaster, GI45550422, Length=310, Percent_Identity=32.258064516129, Blast_Score=142, Evalue=3e-34,
Organism=Drosophila melanogaster, GI24647881, Length=306, Percent_Identity=31.6993464052288, Blast_Score=103, Evalue=1e-22,
Organism=Drosophila melanogaster, GI24663599, Length=311, Percent_Identity=31.1897106109325, Blast_Score=101, Evalue=8e-22,
Organism=Drosophila melanogaster, GI24663595, Length=284, Percent_Identity=31.6901408450704, Blast_Score=97, Evalue=2e-20,

Paralogues:

None

Copy number: 2640 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2380 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 260 Molecules/Cell In: Stationary Phase

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001557
- InterPro:   IPR022383
- InterPro:   IPR001236
- InterPro:   IPR015955
- InterPro:   IPR011275
- InterPro:   IPR016040 [H]

Pfam domain/function: PF02866 Ldh_1_C; PF00056 Ldh_1_N [H]

EC number: =1.1.1.37 [H]

Molecular weight: Translated: 33453; Mature: 33453

Theoretical pI: Translated: 4.65; Mature: 4.65

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIRRNKISVIGSGFTGATTALYLAQRELGDVVLLDMEEKVGPTKGKALDMQETAPIQGFD
CCCCCEEEEEECCCCHHHHHHHHHHHHHCCEEEEECHHHCCCCCCCEECCHHCCCCCCCH
SWVTGTSDYADTADSDVVVITAGIARKPGMSRDDLVATNANVMRSVVKEIARYSPDAILI
HHCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCEEECCHHHHHHHHHHHHHCCCCEEEE
VLTNPVDAMTYAAFKESGFPKERVIGQSGVLDTARFRTFVAQELNVSVKDVSGFVLGGHG
EEECCHHHHHHHHHHHCCCCHHHHCCCCCCCHHHHHHHHHHHHHCCEEEECCEEEECCCC
DDMVPLVRYSYAGGIPLEKLIPHERIEQIVERTRKGGGEIVQLLGDGSAYYAPAAAIVEM
CCCHHHHHHHCCCCCCHHHHCCHHHHHHHHHHHHCCCCCEEHEECCCCCHHHHHHHHHHH
VEAIVKDQRRILPAIAYLEGEYGFQDLYLGVPTILGKHGIEQVLELDLTDEEKAALSKSA
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
DSVRSVLSVL
HHHHHHHHHC
>Mature Secondary Structure
MIRRNKISVIGSGFTGATTALYLAQRELGDVVLLDMEEKVGPTKGKALDMQETAPIQGFD
CCCCCEEEEEECCCCHHHHHHHHHHHHHCCEEEEECHHHCCCCCCCEECCHHCCCCCCCH
SWVTGTSDYADTADSDVVVITAGIARKPGMSRDDLVATNANVMRSVVKEIARYSPDAILI
HHCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCEEECCHHHHHHHHHHHHHCCCCEEEE
VLTNPVDAMTYAAFKESGFPKERVIGQSGVLDTARFRTFVAQELNVSVKDVSGFVLGGHG
EEECCHHHHHHHHHHHCCCCHHHHCCCCCCCHHHHHHHHHHHHHCCEEEECCEEEECCCC
DDMVPLVRYSYAGGIPLEKLIPHERIEQIVERTRKGGGEIVQLLGDGSAYYAPAAAIVEM
CCCHHHHHHHCCCCCCHHHHCCHHHHHHHHHHHHCCCCCEEHEECCCCCHHHHHHHHHHH
VEAIVKDQRRILPAIAYLEGEYGFQDLYLGVPTILGKHGIEQVLELDLTDEEKAALSKSA
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
DSVRSVLSVL
HHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8694770 [H]