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The map label for this gene is dgkA [H]

Identifier: 229916328

GI number: 229916328

Start: 549092

End: 549439

Strand: Reverse

Name: dgkA [H]

Synonym: EAT1b_0597

Alternate gene names: 229916328

Gene position: 549439-549092 (Counterclockwise)

Preceding gene: 229916329

Following gene: 229916327

Centisome position: 18.32

GC content: 51.72

Gene sequence:

>348_bases
GTGAGACCGTTCCGTGTGGCATGTGAGGGGATCCTCCATGCCGTTCGTTCGGAACGAAACATGCGTCTCCATCTCCTATC
GGCTCTTCTTGTATTCATCTTTTCAATCTGGTTACAGCCGACAAGGCTCGAGACCGTCATCTTGTTCGGGTGGGTGATTG
CCGTCATTAGCATGGAATTGATGAATACGGCAATCGAACGAACGGTAGACCTCGTGACGAAGGACGTCCATCCGCTCGCG
AAGCAAGCGAAAGATGTTGCCGCAGGAGCCGTTTTAGTCGCTTCATTTGGTGCTGCCGTAACGGCGCTCATAATATTCGG
TCCACGGCTATGGAACATGCTATACTAA

Upstream 100 bases:

>100_bases
ATTTGCTTGGGTATGATCATATGACACCCGAGGAAGAAGCGGAGATGACGGCAAGGCAAGAAGAAGTGCTCACTCATTTT
GAGTTGAAGCGAGGCAACGT

Downstream 100 bases:

>100_bases
TAGTTCTGAAATTGAGAAAGCAGGTGCAATATGTATCAAGAAGATTTTAAATCCGGATTCGTCTCGATTATCGGGCGACC
GAACGTTGGAAAATCGACAT

Product: diacylglycerol kinase

Products: NA

Alternate protein names: UdpK [H]

Number of amino acids: Translated: 115; Mature: 115

Protein sequence:

>115_residues
MRPFRVACEGILHAVRSERNMRLHLLSALLVFIFSIWLQPTRLETVILFGWVIAVISMELMNTAIERTVDLVTKDVHPLA
KQAKDVAAGAVLVASFGAAVTALIIFGPRLWNMLY

Sequences:

>Translated_115_residues
MRPFRVACEGILHAVRSERNMRLHLLSALLVFIFSIWLQPTRLETVILFGWVIAVISMELMNTAIERTVDLVTKDVHPLA
KQAKDVAAGAVLVASFGAAVTALIIFGPRLWNMLY
>Mature_115_residues
MRPFRVACEGILHAVRSERNMRLHLLSALLVFIFSIWLQPTRLETVILFGWVIAVISMELMNTAIERTVDLVTKDVHPLA
KQAKDVAAGAVLVASFGAAVTALIIFGPRLWNMLY

Specific function: Catalyzes the phosphorylation of undecaprenol, which is probably the primary physiological substrate. Is also able to phosphorylate diacylglycerol, albeit with very low efficiency. May play a role in adaptability to environmental stress conditions such as

COG id: COG0818

COG function: function code M; Diacylglycerol kinase

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial diacylglycerol kinase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000829 [H]

Pfam domain/function: PF01219 DAGK_prokar [H]

EC number: =2.7.1.66 [H]

Molecular weight: Translated: 12846; Mature: 12846

Theoretical pI: Translated: 10.12; Mature: 10.12

Prosite motif: PS00024 HEMOPEXIN ; PS01069 DAGK_PROKAR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRPFRVACEGILHAVRSERNMRLHLLSALLVFIFSIWLQPTRLETVILFGWVIAVISMEL
CCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
MNTAIERTVDLVTKDVHPLAKQAKDVAAGAVLVASFGAAVTALIIFGPRLWNMLY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MRPFRVACEGILHAVRSERNMRLHLLSALLVFIFSIWLQPTRLETVILFGWVIAVISMEL
CCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
MNTAIERTVDLVTKDVHPLAKQAKDVAAGAVLVASFGAAVTALIIFGPRLWNMLY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8407794; 9422608; 12397186 [H]