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The map label for this gene is era [H]

Identifier: 229916327

GI number: 229916327

Start: 548153

End: 549061

Strand: Reverse

Name: era [H]

Synonym: EAT1b_0596

Alternate gene names: 229916327

Gene position: 549061-548153 (Counterclockwise)

Preceding gene: 229916328

Following gene: 229916326

Centisome position: 18.3

GC content: 46.64

Gene sequence:

>909_bases
ATGTATCAAGAAGATTTTAAATCCGGATTCGTCTCGATTATCGGGCGACCGAACGTTGGAAAATCGACATTCTTAAACCG
TGTGATCGGACAAAAAATTGCCATCATGTCGGATAAACCTCAAACGACACGAAATAAGGTGCAAGGTGTGTACACAACCG
ATGATTCCCAAATCATCTTCATCGATACACCAGGGATTCATAAACCGAAACACCGCCTTGGCGACTTCATGATGAAAGTG
GCGACGAACGCACTACGCGAAGTCGATGCGATTCTCTTCATGGTGAACGTGACGGAACCGCGTGGAGCCGGTGATGACTT
CATTATTGAGAAACTCAAAGGGCTCGACACACCGATTATCCTCGTTATGAACAAGATTGATTTGATTCATCCGGATGAAC
TTCCGAAAGTGATCGAACAATATACGAACGAATTGGATTTCGCGGCGTACGTCCCGATTTCGGCGCTACAAGGCAACAAC
GTCGAACCGCTCTTAGGTGAGATCAAGAAATTGTTACCGGAAGGACCGATGTATTATCCGGCCGATCAAGTGACGGACCA
CCCGGAGCGATTCATCATTTCGGAGATGATTCGTGAAAAAGTGTTGCATAAGACACGCGATGAAGTCCCTCACTCGATTG
CTGTAGCCATTGAATCGATCAAGAAACGCGAGAACTCTGAGATGATCGATGTCGAAGCGGTGATTATGGTGGAGCGTGAT
TCGCAAAAAGGAATCATCATCGGAAAAAAAGGCGCCATGCTTCGTGAAATCGGAACAGACGCGCGCCATGATATCGAAGC
GTTGCTCGGAACTAAAGTTTATTTAAATCTATGGGTAAAAGTGCAGAAAGACTGGCGTAACAAGATGGGTCAGCTTCGTG
ACCTCGGTTTCCGCGACGACGAGTATTGA

Upstream 100 bases:

>100_bases
CGCTTCATTTGGTGCTGCCGTAACGGCGCTCATAATATTCGGTCCACGGCTATGGAACATGCTATACTAATAGTTCTGAA
ATTGAGAAAGCAGGTGCAAT

Downstream 100 bases:

>100_bases
GCCATGTTACAAAAAGTCACGGGAATCGTCGTTCGTACCGTAAATTATGGGGAATCGAATAAAGTCGTGACATTGTTTAC
AGAGGAACTTGGCAAAGTCG

Product: GTP-binding protein Era

Products: NA

Alternate protein names: Bex protein [H]

Number of amino acids: Translated: 302; Mature: 302

Protein sequence:

>302_residues
MYQEDFKSGFVSIIGRPNVGKSTFLNRVIGQKIAIMSDKPQTTRNKVQGVYTTDDSQIIFIDTPGIHKPKHRLGDFMMKV
ATNALREVDAILFMVNVTEPRGAGDDFIIEKLKGLDTPIILVMNKIDLIHPDELPKVIEQYTNELDFAAYVPISALQGNN
VEPLLGEIKKLLPEGPMYYPADQVTDHPERFIISEMIREKVLHKTRDEVPHSIAVAIESIKKRENSEMIDVEAVIMVERD
SQKGIIIGKKGAMLREIGTDARHDIEALLGTKVYLNLWVKVQKDWRNKMGQLRDLGFRDDEY

Sequences:

>Translated_302_residues
MYQEDFKSGFVSIIGRPNVGKSTFLNRVIGQKIAIMSDKPQTTRNKVQGVYTTDDSQIIFIDTPGIHKPKHRLGDFMMKV
ATNALREVDAILFMVNVTEPRGAGDDFIIEKLKGLDTPIILVMNKIDLIHPDELPKVIEQYTNELDFAAYVPISALQGNN
VEPLLGEIKKLLPEGPMYYPADQVTDHPERFIISEMIREKVLHKTRDEVPHSIAVAIESIKKRENSEMIDVEAVIMVERD
SQKGIIIGKKGAMLREIGTDARHDIEALLGTKVYLNLWVKVQKDWRNKMGQLRDLGFRDDEY
>Mature_302_residues
MYQEDFKSGFVSIIGRPNVGKSTFLNRVIGQKIAIMSDKPQTTRNKVQGVYTTDDSQIIFIDTPGIHKPKHRLGDFMMKV
ATNALREVDAILFMVNVTEPRGAGDDFIIEKLKGLDTPIILVMNKIDLIHPDELPKVIEQYTNELDFAAYVPISALQGNN
VEPLLGEIKKLLPEGPMYYPADQVTDHPERFIISEMIREKVLHKTRDEVPHSIAVAIESIKKRENSEMIDVEAVIMVERD
SQKGIIIGKKGAMLREIGTDARHDIEALLGTKVYLNLWVKVQKDWRNKMGQLRDLGFRDDEY

Specific function: An essential GTPase that binds both GDP and GTP, with rapid nucleotide exchange. Plays a role in 16S rRNA processing and 30S ribosomal subunit biogenesis and possibly also in cell cycle regulation and energy metabolism. Binds both GDP and GTP. Complements

COG id: COG1159

COG function: function code R; GTPase

Gene ontology:

Cell location: Cytoplasm. Cell membrane; Peripheral membrane protein [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 KH type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI24307899, Length=324, Percent_Identity=25, Blast_Score=84, Evalue=2e-16,
Organism=Escherichia coli, GI1788919, Length=296, Percent_Identity=39.527027027027, Blast_Score=224, Evalue=6e-60,
Organism=Escherichia coli, GI87082120, Length=179, Percent_Identity=32.9608938547486, Blast_Score=88, Evalue=9e-19,
Organism=Caenorhabditis elegans, GI17532761, Length=146, Percent_Identity=30.8219178082192, Blast_Score=65, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6323665, Length=137, Percent_Identity=30.6569343065693, Blast_Score=63, Evalue=7e-11,
Organism=Drosophila melanogaster, GI24646434, Length=310, Percent_Identity=25.4838709677419, Blast_Score=93, Evalue=2e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005662
- InterPro:   IPR006073
- InterPro:   IPR015946
- InterPro:   IPR009019
- InterPro:   IPR004044
- InterPro:   IPR002917
- InterPro:   IPR005225 [H]

Pfam domain/function: PF07650 KH_2; PF01926 MMR_HSR1 [H]

EC number: NA

Molecular weight: Translated: 34328; Mature: 34328

Theoretical pI: Translated: 6.19; Mature: 6.19

Prosite motif: PS50823 KH_TYPE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYQEDFKSGFVSIIGRPNVGKSTFLNRVIGQKIAIMSDKPQTTRNKVQGVYTTDDSQIIF
CCHHHHHCCCEEEECCCCCCHHHHHHHHHHCCEEEECCCCCHHHHHCCEEEECCCCEEEE
IDTPGIHKPKHRLGDFMMKVATNALREVDAILFMVNVTEPRGAGDDFIIEKLKGLDTPII
EECCCCCCHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCCCCHHHHHHHHHCCCCCEE
LVMNKIDLIHPDELPKVIEQYTNELDFAAYVPISALQGNNVEPLLGEIKKLLPEGPMYYP
EEEECCCCCCCHHHHHHHHHHHCCCCEEEECCHHHCCCCCCCHHHHHHHHHCCCCCCCCC
ADQVTDHPERFIISEMIREKVLHKTRDEVPHSIAVAIESIKKRENSEMIDVEAVIMVERD
CHHCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCEEEEEEEEEEEEC
SQKGIIIGKKGAMLREIGTDARHDIEALLGTKVYLNLWVKVQKDWRNKMGQLRDLGFRDD
CCCCEEEECCCCHHHHHCCCHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHHCCCCCC
EY
CC
>Mature Secondary Structure
MYQEDFKSGFVSIIGRPNVGKSTFLNRVIGQKIAIMSDKPQTTRNKVQGVYTTDDSQIIF
CCHHHHHCCCEEEECCCCCCHHHHHHHHHHCCEEEECCCCCHHHHHCCEEEECCCCEEEE
IDTPGIHKPKHRLGDFMMKVATNALREVDAILFMVNVTEPRGAGDDFIIEKLKGLDTPII
EECCCCCCHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCCCCHHHHHHHHHCCCCCEE
LVMNKIDLIHPDELPKVIEQYTNELDFAAYVPISALQGNNVEPLLGEIKKLLPEGPMYYP
EEEECCCCCCCHHHHHHHHHHHCCCCEEEECCHHHCCCCCCCHHHHHHHHHCCCCCCCCC
ADQVTDHPERFIISEMIREKVLHKTRDEVPHSIAVAIESIKKRENSEMIDVEAVIMVERD
CHHCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCEEEEEEEEEEEEC
SQKGIIIGKKGAMLREIGTDARHDIEALLGTKVYLNLWVKVQKDWRNKMGQLRDLGFRDD
CCCCEEEECCCCHHHHHCCCHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHHCCCCCC
EY
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8969508; 9384377; 2526291 [H]