Click here to switch to the map view.

The map label for this gene is folD

Identifier: 229916253

GI number: 229916253

Start: 477011

End: 477847

Strand: Reverse

Name: folD

Synonym: EAT1b_0522

Alternate gene names: 229916253

Gene position: 477847-477011 (Counterclockwise)

Preceding gene: 229916254

Following gene: 229916252

Centisome position: 15.93

GC content: 50.54

Gene sequence:

>837_bases
ATGGCAGTAGTGATCGACGGGAAACAAGTGGCAGCATCATATCGAGAAACGTTAAAAGAGCGTGTGGCGGAACTTCGCGC
ACGTGGCATCGTACCGAAATTAAAAGTAGTGTTGATTGGGGATGACCCAGCGAGTCATAGCTATGTACGCGGGAAGGAAC
GGGCGGCAGAAGAAATCGGAATCGATTCACAAGTCCTCCGTTTTGATGAGACGATTAGCGAGAAGGAATTGCTCGACTTG
ATTGATTTGATGAATGCGGATGAGGAAGTTCATGGGATTCTCGTGCAACTTCCTTTGCCAAAACACATCGACGAATCTCG
AGTCATCATGCGTATCTCCCCTGAAAAGGACGTCGATGGCTTTCATCCTGAGAATGTCGGGAAGATGATGCTCGGTCTGG
ATACGTTACTTCCGTGCACGCCGCACGGTATCTTACATCTCGCAAAAACACAAACCGAGATCGCCGGAAAGCACGTTGTC
GTCGTCGGTCGTAGTCAAATCGTCGGGAAACCGGTAGGCATGTTGTTCTTGAATGAATCAGCAACGGTCACGTATTGTCA
TTCGAAAACAGCTGACCTCGGTGAGATGACGCGACAAGCGGATATTTTGATTGTCGCGGTCGGACGCGCTGGTCTAGTCA
CATCAGACATGGTCAAACCAGGCGCGCTTGTCATCGATGTCGGAGTCAATCGGGTCGAAGGTCGTCTAGTCGGTGACGTG
GACTATGAAGCTGTTAAGGAAAAGGCAAGTGCCATCACCCCAGTTCCGGGTGGTGTCGGTCCGATGACCATTACGATGCT
CATGCATAATACGGTCGAGGTTGCATCACGTGGCTAA

Upstream 100 bases:

>100_bases
GAATGGCGTACTCGGAAATATCATTAAAGAAGACTAAAAAATAGAGATAAGACACCAGACCAACTACAGAAAAACCTAAA
GACAACGGGGGATGAGAGAG

Downstream 100 bases:

>100_bases
TCCACTTCCGGTTTCTGAAGTTGTTCGGTATGTGAAGCGACAACTTGATGATGATGTATTGCTTCGACAAGTCGCGGTCA
TCGGTGAAATCTCAAATTTT

Product: bifunctional 5,10-methylene-tetrahydrofolate dehydrogenase/ 5,10-methylene-tetrahydrofolate cyclohydrolase

Products: NA

Alternate protein names: Methylenetetrahydrofolate dehydrogenase; Methenyltetrahydrofolate cyclohydrolase

Number of amino acids: Translated: 278; Mature: 277

Protein sequence:

>278_residues
MAVVIDGKQVAASYRETLKERVAELRARGIVPKLKVVLIGDDPASHSYVRGKERAAEEIGIDSQVLRFDETISEKELLDL
IDLMNADEEVHGILVQLPLPKHIDESRVIMRISPEKDVDGFHPENVGKMMLGLDTLLPCTPHGILHLAKTQTEIAGKHVV
VVGRSQIVGKPVGMLFLNESATVTYCHSKTADLGEMTRQADILIVAVGRAGLVTSDMVKPGALVIDVGVNRVEGRLVGDV
DYEAVKEKASAITPVPGGVGPMTITMLMHNTVEVASRG

Sequences:

>Translated_278_residues
MAVVIDGKQVAASYRETLKERVAELRARGIVPKLKVVLIGDDPASHSYVRGKERAAEEIGIDSQVLRFDETISEKELLDL
IDLMNADEEVHGILVQLPLPKHIDESRVIMRISPEKDVDGFHPENVGKMMLGLDTLLPCTPHGILHLAKTQTEIAGKHVV
VVGRSQIVGKPVGMLFLNESATVTYCHSKTADLGEMTRQADILIVAVGRAGLVTSDMVKPGALVIDVGVNRVEGRLVGDV
DYEAVKEKASAITPVPGGVGPMTITMLMHNTVEVASRG
>Mature_277_residues
AVVIDGKQVAASYRETLKERVAELRARGIVPKLKVVLIGDDPASHSYVRGKERAAEEIGIDSQVLRFDETISEKELLDLI
DLMNADEEVHGILVQLPLPKHIDESRVIMRISPEKDVDGFHPENVGKMMLGLDTLLPCTPHGILHLAKTQTEIAGKHVVV
VGRSQIVGKPVGMLFLNESATVTYCHSKTADLGEMTRQADILIVAVGRAGLVTSDMVKPGALVIDVGVNRVEGRLVGDVD
YEAVKEKASAITPVPGGVGPMTITMLMHNTVEVASRG

Specific function: Catalyzes the oxidation of 5,10- methylenetetrahydrofolate to 5,10-methenyltetrahydrofolate and then the hydrolysis of 5,10-methenyltetrahydrofolate to 10- formyltetrahydrofolate

COG id: COG0190

COG function: function code H; 5,10-methylene-tetrahydrofolate dehydrogenase/Methenyl tetrahydrofolate cyclohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family

Homologues:

Organism=Homo sapiens, GI222136639, Length=290, Percent_Identity=47.9310344827586, Blast_Score=246, Evalue=1e-65,
Organism=Homo sapiens, GI94721354, Length=294, Percent_Identity=48.9795918367347, Blast_Score=242, Evalue=3e-64,
Organism=Homo sapiens, GI222418558, Length=294, Percent_Identity=46.5986394557823, Blast_Score=237, Evalue=7e-63,
Organism=Homo sapiens, GI36796743, Length=209, Percent_Identity=25.8373205741627, Blast_Score=66, Evalue=4e-11,
Organism=Escherichia coli, GI1786741, Length=277, Percent_Identity=48.3754512635379, Blast_Score=250, Evalue=8e-68,
Organism=Caenorhabditis elegans, GI17568735, Length=256, Percent_Identity=47.265625, Blast_Score=213, Evalue=6e-56,
Organism=Saccharomyces cerevisiae, GI6319558, Length=286, Percent_Identity=42.6573426573427, Blast_Score=235, Evalue=5e-63,
Organism=Saccharomyces cerevisiae, GI6321643, Length=294, Percent_Identity=42.1768707482993, Blast_Score=226, Evalue=3e-60,
Organism=Saccharomyces cerevisiae, GI6322933, Length=315, Percent_Identity=25.7142857142857, Blast_Score=83, Evalue=6e-17,
Organism=Drosophila melanogaster, GI17136816, Length=295, Percent_Identity=45.4237288135593, Blast_Score=242, Evalue=2e-64,
Organism=Drosophila melanogaster, GI17136818, Length=295, Percent_Identity=45.4237288135593, Blast_Score=241, Evalue=3e-64,
Organism=Drosophila melanogaster, GI62472483, Length=289, Percent_Identity=48.0968858131488, Blast_Score=241, Evalue=4e-64,
Organism=Drosophila melanogaster, GI45551871, Length=289, Percent_Identity=48.0968858131488, Blast_Score=241, Evalue=4e-64,
Organism=Drosophila melanogaster, GI24645718, Length=289, Percent_Identity=48.0968858131488, Blast_Score=241, Evalue=5e-64,
Organism=Drosophila melanogaster, GI17137370, Length=289, Percent_Identity=48.0968858131488, Blast_Score=241, Evalue=5e-64,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): FOLD_EXISA (C4L3F6)

Other databases:

- EMBL:   CP001615
- RefSeq:   YP_002884899.1
- GeneID:   7871256
- GenomeReviews:   CP001615_GR
- KEGG:   eat:EAT1b_0522
- OMA:   MIMIERI
- ProtClustDB:   PRK14190
- GO:   GO:0005488
- HAMAP:   MF_01576
- InterPro:   IPR016040
- InterPro:   IPR000672
- InterPro:   IPR020630
- InterPro:   IPR020867
- InterPro:   IPR020631
- Gene3D:   G3DSA:3.40.50.720
- PRINTS:   PR00085

Pfam domain/function: PF00763 THF_DHG_CYH; PF02882 THF_DHG_CYH_C

EC number: =1.5.1.5; =3.5.4.9

Molecular weight: Translated: 30003; Mature: 29872

Theoretical pI: Translated: 5.83; Mature: 5.83

Prosite motif: PS00766 THF_DHG_CYH_1; PS00767 THF_DHG_CYH_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAVVIDGKQVAASYRETLKERVAELRARGIVPKLKVVLIGDDPASHSYVRGKERAAEEIG
CEEEECCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCHHCCHHHHHHHHC
IDSQVLRFDETISEKELLDLIDLMNADEEVHGILVQLPLPKHIDESRVIMRISPEKDVDG
CCHHHHHHHHHCCHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCEEEEEECCCCCCCC
FHPENVGKMMLGLDTLLPCTPHGILHLAKTQTEIAGKHVVVVGRSQIVGKPVGMLFLNES
CCCCHHHHHHHHHHHHCCCCCCHHEEEHHHHHHHCCCEEEEEECCHHCCCCEEEEEECCC
ATVTYCHSKTADLGEMTRQADILIVAVGRAGLVTSDMVKPGALVIDVGVNRVEGRLVGDV
CEEEEECCCCCCHHHHHCCCCEEEEEECCCCCEEHHCCCCCCEEEEECCCCCCCEEEECC
DYEAVKEKASAITPVPGGVGPMTITMLMHNTVEVASRG
CHHHHHHHHHCCCCCCCCCCHHEEEEHHHHHHHHCCCC
>Mature Secondary Structure 
AVVIDGKQVAASYRETLKERVAELRARGIVPKLKVVLIGDDPASHSYVRGKERAAEEIG
EEEECCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCHHCCHHHHHHHHC
IDSQVLRFDETISEKELLDLIDLMNADEEVHGILVQLPLPKHIDESRVIMRISPEKDVDG
CCHHHHHHHHHCCHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCEEEEEECCCCCCCC
FHPENVGKMMLGLDTLLPCTPHGILHLAKTQTEIAGKHVVVVGRSQIVGKPVGMLFLNES
CCCCHHHHHHHHHHHHCCCCCCHHEEEHHHHHHHCCCEEEEEECCHHCCCCEEEEEECCC
ATVTYCHSKTADLGEMTRQADILIVAVGRAGLVTSDMVKPGALVIDVGVNRVEGRLVGDV
CEEEEECCCCCCHHHHHCCCCEEEEEECCCCCEEHHCCCCCCEEEEECCCCCCCEEEECC
DYEAVKEKASAITPVPGGVGPMTITMLMHNTVEVASRG
CHHHHHHHHHCCCCCCCCCCHHEEEEHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA