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The map label for this gene is nusB

Identifier: 229916254

GI number: 229916254

Start: 477911

End: 478297

Strand: Reverse

Name: nusB

Synonym: EAT1b_0523

Alternate gene names: 229916254

Gene position: 478297-477911 (Counterclockwise)

Preceding gene: 229916255

Following gene: 229916253

Centisome position: 15.94

GC content: 44.7

Gene sequence:

>387_bases
ATGAAACGTCATGAAGCACGAGAGAAAGCGATTCAAACGCTTTTTCAAATCGAAGTATCTAAATTAGAGGTGGACGAAGC
AATCGAGTTCGCATTGGATGGAGAAGAGTCTGATCCGTTTTACGAACAACTCGTCACAGGGACGCTAGAACATATTGAAG
ATATCGACGCATTACTAGTTGAAAACTTGAAAAACTGGCGCTTGGATCGCCTAGGAAACGTCGAGCGTACGATTCTTCGA
ATGGCAACGTTCGAATTGTTATACGTCGAGACGATTCCAGAGAACGTCACGATTAACGAGGCGGTCGAACTCGCGAAATC
GTTCGGAGACGAAGAGGCCGGTAAACTCGTGAATGGCGTACTCGGAAATATCATTAAAGAAGACTAA

Upstream 100 bases:

>100_bases
AGCCCGAACATTATTTCGGTTTCCTGGTTTTTTGTACTGAATTTATGAGTGCTTTATGCTAAACTATCACAATGAAAGCA
TGTAAAAGGAGAGTAACTGA

Downstream 100 bases:

>100_bases
AAAATAGAGATAAGACACCAGACCAACTACAGAAAAACCTAAAGACAACGGGGGATGAGAGAGATGGCAGTAGTGATCGA
CGGGAAACAAGTGGCAGCAT

Product: NusB antitermination factor

Products: NA

Alternate protein names: Protein nusB

Number of amino acids: Translated: 128; Mature: 128

Protein sequence:

>128_residues
MKRHEAREKAIQTLFQIEVSKLEVDEAIEFALDGEESDPFYEQLVTGTLEHIEDIDALLVENLKNWRLDRLGNVERTILR
MATFELLYVETIPENVTINEAVELAKSFGDEEAGKLVNGVLGNIIKED

Sequences:

>Translated_128_residues
MKRHEAREKAIQTLFQIEVSKLEVDEAIEFALDGEESDPFYEQLVTGTLEHIEDIDALLVENLKNWRLDRLGNVERTILR
MATFELLYVETIPENVTINEAVELAKSFGDEEAGKLVNGVLGNIIKED
>Mature_128_residues
MKRHEAREKAIQTLFQIEVSKLEVDEAIEFALDGEESDPFYEQLVTGTLEHIEDIDALLVENLKNWRLDRLGNVERTILR
MATFELLYVETIPENVTINEAVELAKSFGDEEAGKLVNGVLGNIIKED

Specific function: Involved in the transcription termination process

COG id: COG0781

COG function: function code K; Transcription termination factor

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nusB family

Homologues:

Organism=Escherichia coli, GI1786618, Length=118, Percent_Identity=36.4406779661017, Blast_Score=70, Evalue=6e-14,

Paralogues:

None

Copy number: 900 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NUSB_EXISA (C4L3F7)

Other databases:

- EMBL:   CP001615
- RefSeq:   YP_002884900.1
- GeneID:   7871257
- GenomeReviews:   CP001615_GR
- KEGG:   eat:EAT1b_0523
- OMA:   FAYDYDK
- HAMAP:   MF_00073
- InterPro:   IPR011605
- InterPro:   IPR006027
- Gene3D:   G3DSA:1.10.940.10
- TIGRFAMs:   TIGR01951

Pfam domain/function: PF01029 NusB; SSF48013 NusB_RsmB_TIM44

EC number: NA

Molecular weight: Translated: 14589; Mature: 14589

Theoretical pI: Translated: 4.09; Mature: 4.09

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRHEAREKAIQTLFQIEVSKLEVDEAIEFALDGEESDPFYEQLVTGTLEHIEDIDALLV
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHH
ENLKNWRLDRLGNVERTILRMATFELLYVETIPENVTINEAVELAKSFGDEEAGKLVNGV
HHHHHCCHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCHHHHHHHHHH
LGNIIKED
HHHHHCCC
>Mature Secondary Structure
MKRHEAREKAIQTLFQIEVSKLEVDEAIEFALDGEESDPFYEQLVTGTLEHIEDIDALLV
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHH
ENLKNWRLDRLGNVERTILRMATFELLYVETIPENVTINEAVELAKSFGDEEAGKLVNGV
HHHHHCCHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCHHHHHHHHHH
LGNIIKED
HHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA