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The map label for this gene is xseA

Identifier: 229916252

GI number: 229916252

Start: 475672

End: 477018

Strand: Reverse

Name: xseA

Synonym: EAT1b_0521

Alternate gene names: 229916252

Gene position: 477018-475672 (Counterclockwise)

Preceding gene: 229916253

Following gene: 229916251

Centisome position: 15.9

GC content: 50.48

Gene sequence:

>1347_bases
GTGGCTAATCCACTTCCGGTTTCTGAAGTTGTTCGGTATGTGAAGCGACAACTTGATGATGATGTATTGCTTCGACAAGT
CGCGGTCATCGGTGAAATCTCAAATTTTAAACGGTATTCATCCGGTCATTGTTATTTCACGTTGAAAGATGACGCGTCAC
GGATGAAAGCCGTCATGTTTTCTCGCGATGCCAAACAACTTCAATTCGAACCGAAAGATGGAATGAAAGTCATCGCCGTC
TCGAAGGTGACGATGTATGAAGCGACGGGAGATGTACAACTGTACGTCGAATTGATGCGTCAAGACGGAATCGGTCTATT
GTTTGAGCGATATGAAGCGAGAAAGCGTGAACTAGAGGAAATGGGCTGGTTCGATGACGAGCGGAAGAAACCGCTCCCGA
TGTTTCCAGAGCGCGTTGGGATCGTGACGTCTCCAAAAGGGGCGGCGCTACACGATATCGCGACCACACTTCGTCGTCGT
GCGCCACATGTGGCCATCACGTTCGCACCTGTAGCTGTACAGGGAGAGATGGCGGCACCACAAGTCGCGTCGGCCATTCG
CTGGATGAACGAACGCACCGATTGTGATGTACTCATCGTCGGTCGCGGTGGCGGTTCCATCGAAGAGCTTTGGGCTTTCA
ATGAGGATGTTGTGGTAGAGGCGATTTATGCGTCCACGATTCCCATCATTTCTGCGGTCGGTCATGAGACGGACTTTACG
CTTTCTGATTTCGTCGCCGATGTTCGAGCTGCGACTCCGACAGCGGCGGCTGAACTCGCTACGGCGATGATTGATGCGCA
GCGAAAAGATGTAGAACGTTTAGACAACCATCTACACAAAGCTGTCAGAAGTCAGTTGGATGAGTCAAGGAGCCGCGTGG
AACGGATGATCAATAGTTATGGATTAAAATCGCCGAGGTACACGATTTCTCAAAAACGGGAACGATTCGCCCAGTCAGAA
ATCCGTCTCGAGCAAGGGATGAGGCGACATCTCACCCAAGCCACTCATCAGCTCCGTCAGTTGTCACAGCAGCTAGATGT
AAAGCGATTTTCAAAAACGTTGTCTAAGCAAGGGGACGAAGTCAATCATATGGTCGAGCGACTCCGTCGAACGAGACCAT
TAGAACAAGCAACGCTACAATTTGCACAACAGGTTGGACGGTTGCATGCAGTCAGCCCGCTCGCCGTCTTGTCACGTGGA
TATACGTTCATCGAACAAGATGGGGCCTATGTGCAGAACGTGAAACAATTACGCGATGGTGACGTCTCGATTCGTTTTCG
GGATGGTCATGCGATCGCTGAGGTGAAGGAGAGGATTGTCGGTGACGAAGAAAGAACAGACATTTGA

Upstream 100 bases:

>100_bases
GCTGTTAAGGAAAAGGCAAGTGCCATCACCCCAGTTCCGGGTGGTGTCGGTCCGATGACCATTACGATGCTCATGCATAA
TACGGTCGAGGTTGCATCAC

Downstream 100 bases:

>100_bases
GGCGGCACTTGAGCGCCTCGAACAGATCGTCGCGCAACTCGAGAGCGGTGATGTCCCATTAGAGGAAGCGATGACCCTTT
ATGAAGAAGGTGTCCGCTTG

Product: exodeoxyribonuclease VII, large subunit

Products: NA

Alternate protein names: Exodeoxyribonuclease VII large subunit; Exonuclease VII large subunit

Number of amino acids: Translated: 448; Mature: 447

Protein sequence:

>448_residues
MANPLPVSEVVRYVKRQLDDDVLLRQVAVIGEISNFKRYSSGHCYFTLKDDASRMKAVMFSRDAKQLQFEPKDGMKVIAV
SKVTMYEATGDVQLYVELMRQDGIGLLFERYEARKRELEEMGWFDDERKKPLPMFPERVGIVTSPKGAALHDIATTLRRR
APHVAITFAPVAVQGEMAAPQVASAIRWMNERTDCDVLIVGRGGGSIEELWAFNEDVVVEAIYASTIPIISAVGHETDFT
LSDFVADVRAATPTAAAELATAMIDAQRKDVERLDNHLHKAVRSQLDESRSRVERMINSYGLKSPRYTISQKRERFAQSE
IRLEQGMRRHLTQATHQLRQLSQQLDVKRFSKTLSKQGDEVNHMVERLRRTRPLEQATLQFAQQVGRLHAVSPLAVLSRG
YTFIEQDGAYVQNVKQLRDGDVSIRFRDGHAIAEVKERIVGDEERTDI

Sequences:

>Translated_448_residues
MANPLPVSEVVRYVKRQLDDDVLLRQVAVIGEISNFKRYSSGHCYFTLKDDASRMKAVMFSRDAKQLQFEPKDGMKVIAV
SKVTMYEATGDVQLYVELMRQDGIGLLFERYEARKRELEEMGWFDDERKKPLPMFPERVGIVTSPKGAALHDIATTLRRR
APHVAITFAPVAVQGEMAAPQVASAIRWMNERTDCDVLIVGRGGGSIEELWAFNEDVVVEAIYASTIPIISAVGHETDFT
LSDFVADVRAATPTAAAELATAMIDAQRKDVERLDNHLHKAVRSQLDESRSRVERMINSYGLKSPRYTISQKRERFAQSE
IRLEQGMRRHLTQATHQLRQLSQQLDVKRFSKTLSKQGDEVNHMVERLRRTRPLEQATLQFAQQVGRLHAVSPLAVLSRG
YTFIEQDGAYVQNVKQLRDGDVSIRFRDGHAIAEVKERIVGDEERTDI
>Mature_447_residues
ANPLPVSEVVRYVKRQLDDDVLLRQVAVIGEISNFKRYSSGHCYFTLKDDASRMKAVMFSRDAKQLQFEPKDGMKVIAVS
KVTMYEATGDVQLYVELMRQDGIGLLFERYEARKRELEEMGWFDDERKKPLPMFPERVGIVTSPKGAALHDIATTLRRRA
PHVAITFAPVAVQGEMAAPQVASAIRWMNERTDCDVLIVGRGGGSIEELWAFNEDVVVEAIYASTIPIISAVGHETDFTL
SDFVADVRAATPTAAAELATAMIDAQRKDVERLDNHLHKAVRSQLDESRSRVERMINSYGLKSPRYTISQKRERFAQSEI
RLEQGMRRHLTQATHQLRQLSQQLDVKRFSKTLSKQGDEVNHMVERLRRTRPLEQATLQFAQQVGRLHAVSPLAVLSRGY
TFIEQDGAYVQNVKQLRDGDVSIRFRDGHAIAEVKERIVGDEERTDI

Specific function: Bidirectionally degrades single-stranded DNA into large acid-insoluble oligonucleotides, which are then degraded further into small acid-soluble oligonucleotides

COG id: COG1570

COG function: function code L; Exonuclease VII, large subunit

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the xseA family

Homologues:

Organism=Escherichia coli, GI1788856, Length=422, Percent_Identity=39.3364928909953, Blast_Score=284, Evalue=1e-77,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): EX7L_EXISA (C4L3F5)

Other databases:

- EMBL:   CP001615
- RefSeq:   YP_002884898.1
- GeneID:   7871255
- GenomeReviews:   CP001615_GR
- KEGG:   eat:EAT1b_0521
- OMA:   SAVGHER
- ProtClustDB:   CLSK2489196
- GO:   GO:0005737
- HAMAP:   MF_00378
- InterPro:   IPR003753
- InterPro:   IPR020579
- InterPro:   IPR004365
- TIGRFAMs:   TIGR00237

Pfam domain/function: PF02601 Exonuc_VII_L; PF01336 tRNA_anti

EC number: =3.1.11.6

Molecular weight: Translated: 50857; Mature: 50726

Theoretical pI: Translated: 8.23; Mature: 8.23

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANPLPVSEVVRYVKRQLDDDVLLRQVAVIGEISNFKRYSSGHCYFTLKDDASRMKAVMF
CCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHH
SRDAKQLQFEPKDGMKVIAVSKVTMYEATGDVQLYVELMRQDGIGLLFERYEARKRELEE
HCCHHHCCCCCCCCCEEEEEEEEEEEECCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
MGWFDDERKKPLPMFPERVGIVTSPKGAALHDIATTLRRRAPHVAITFAPVAVQGEMAAP
CCCCCCCCCCCCCCCHHHCCEEECCCCCHHHHHHHHHHHCCCCEEEEEECEEECCCCCCH
QVASAIRWMNERTDCDVLIVGRGGGSIEELWAFNEDVVVEAIYASTIPIISAVGHETDFT
HHHHHHHHHCCCCCCCEEEEECCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCC
LSDFVADVRAATPTAAAELATAMIDAQRKDVERLDNHLHKAVRSQLDESRSRVERMINSY
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GLKSPRYTISQKRERFAQSEIRLEQGMRRHLTQATHQLRQLSQQLDVKRFSKTLSKQGDE
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCH
VNHMVERLRRTRPLEQATLQFAQQVGRLHAVSPLAVLSRGYTFIEQDGAYVQNVKQLRDG
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCHHHHHHCCCCEEECCCHHHHHHHHHCCC
DVSIRFRDGHAIAEVKERIVGDEERTDI
CEEEEEECCCHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
ANPLPVSEVVRYVKRQLDDDVLLRQVAVIGEISNFKRYSSGHCYFTLKDDASRMKAVMF
CCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHH
SRDAKQLQFEPKDGMKVIAVSKVTMYEATGDVQLYVELMRQDGIGLLFERYEARKRELEE
HCCHHHCCCCCCCCCEEEEEEEEEEEECCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
MGWFDDERKKPLPMFPERVGIVTSPKGAALHDIATTLRRRAPHVAITFAPVAVQGEMAAP
CCCCCCCCCCCCCCCHHHCCEEECCCCCHHHHHHHHHHHCCCCEEEEEECEEECCCCCCH
QVASAIRWMNERTDCDVLIVGRGGGSIEELWAFNEDVVVEAIYASTIPIISAVGHETDFT
HHHHHHHHHCCCCCCCEEEEECCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCC
LSDFVADVRAATPTAAAELATAMIDAQRKDVERLDNHLHKAVRSQLDESRSRVERMINSY
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GLKSPRYTISQKRERFAQSEIRLEQGMRRHLTQATHQLRQLSQQLDVKRFSKTLSKQGDE
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCH
VNHMVERLRRTRPLEQATLQFAQQVGRLHAVSPLAVLSRGYTFIEQDGAYVQNVKQLRDG
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCHHHHHHCCCCEEECCCHHHHHHHHHCCC
DVSIRFRDGHAIAEVKERIVGDEERTDI
CEEEEEECCCHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA