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The map label for this gene is mepA [H]

Identifier: 229916209

GI number: 229916209

Start: 436698

End: 438095

Strand: Reverse

Name: mepA [H]

Synonym: EAT1b_0478

Alternate gene names: 229916209

Gene position: 438095-436698 (Counterclockwise)

Preceding gene: 229916210

Following gene: 229916207

Centisome position: 14.6

GC content: 51.07

Gene sequence:

>1398_bases
ATGAACCAAAATACGGAGCAATTACGGACTGAGCCCATCGGCAGACTCCTTTTCAAGTTATCGCTTCCGGCGATGATTGG
GATGCTCGTGACGGCGCTCTATAATATCATTGATACGATTTTTGTCGCCCGGGGGATTGGTGCGTCGGCAGTCGCGGCCA
TCGGGATCGCGTTCCCGATTCAGATGATTTTAATGGCTGTGTCGAGTGCCGTGGGACTGGGTGGGGCGTCCATCAGTTCG
AGGAAGCTCGGACGCGGTGATGATGCCGGTGCAGCCGTTACATTCTTGAACGTACTTGTCCTCGTCGGTCTGTTTGCGGC
ACTTGCGGTCAGTTCCGCTTTCTTTATGTTAGATGACATTTTAAAAGTGTTCGGTGCGACGACCGAGATCATGGATCTCA
GCCGACAGTACTTATCAGTTGTATTAATCAGTTCACCATTCTTCATGTTCACACTCGCTTCGAGTGCGATGGTTCGTGCT
GAAGGGCAGGCACCTTATCAAATGAAGGTCATGCTGACGACCGTCCTCGTCAACCTCGTGACGACCCCACTCTTCATCTT
TACGTTCGACTGGGGTATGTATGGGGCAGCGTCTTCGACGGCACTCGCCCAAGTCATCGGTTCCGTATTGATGCTGCGAT
TCTTCTTCGCCCGAAAACCAAAAACGGAACTGCGCTTCAAGTGGCGTCAGTTCCGGATTCGATTCGATGAGATGAAAGAA
ATCATGGCAATCGGTTCGTCTTCGTTCATCATGATGGTGTCCCAATCTATTTTATTCGTTGGTGTCAACGTGATGCTCGG
TACGTACGGCGGAACCGAAGAATTGGCCATTTTCGCCATTATCAACAAGTTCATGGCGCTCGTCGGTATGCCGATCATGG
GAATCGTCCAAGGGATGCAACCAATCGTAGGATATAACTACGGCGCAGAGCGATTCGACCGAATGCGTGAAACCATTTGG
ACGGCGATGCGTGCCGGGATTGCGATTGCTGTCATCATTTGGTTGATTCTACAGCTTTTCCCGCAAACGCTCATGGGCTG
GTTCTCGACGGATGAGGCGCTCATTGCTGGAGGTGTGACGGCGATTCACATTCTGTTCGCCGTTTCTTTCCTTCCGAGTG
TGCAGATGCTCGTCAATGGAATCTATCAGTCCCTCGGGAAAGCGCGAGTCGCCATGTTCTTGTCTCTGTCACGTCAGACG
CTTTACACGATACCGCTCGTCTTCATTCTCCCATCGTTCCTCGGGGTGTTCGGCGTCTGGTTGGCATTCCCAATCGCTGA
TGCGATGACATTTTTGACGGCAGTCGCCCTGGCATTCTGGGACCGTAAATTATTGTTCCGTCCTTCAGAAGAAGATAAAG
AAGCACATGATGTAAATCCGAAAGCGACAAACTTATAA

Upstream 100 bases:

>100_bases
GCGTGCTCGTGCGCTCGTTTGACACATCCACCGAGCCAACATAGTCGCCCGTTCCGTCCAATCCTGCTACCCTAAGAAGT
AGAGACGGAGGGATGGAAGA

Downstream 100 bases:

>100_bases
TCAAACGTCCGCTCGTGTCGAGCGGGCGTCTTCTTTTAGGAACAAGTATATTCCGCCGAGGAGGACGATCGCACCGACCC
ATTGGAACGGTCCAAGTCGC

Product: MATE efflux family protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 465; Mature: 465

Protein sequence:

>465_residues
MNQNTEQLRTEPIGRLLFKLSLPAMIGMLVTALYNIIDTIFVARGIGASAVAAIGIAFPIQMILMAVSSAVGLGGASISS
RKLGRGDDAGAAVTFLNVLVLVGLFAALAVSSAFFMLDDILKVFGATTEIMDLSRQYLSVVLISSPFFMFTLASSAMVRA
EGQAPYQMKVMLTTVLVNLVTTPLFIFTFDWGMYGAASSTALAQVIGSVLMLRFFFARKPKTELRFKWRQFRIRFDEMKE
IMAIGSSSFIMMVSQSILFVGVNVMLGTYGGTEELAIFAIINKFMALVGMPIMGIVQGMQPIVGYNYGAERFDRMRETIW
TAMRAGIAIAVIIWLILQLFPQTLMGWFSTDEALIAGGVTAIHILFAVSFLPSVQMLVNGIYQSLGKARVAMFLSLSRQT
LYTIPLVFILPSFLGVFGVWLAFPIADAMTFLTAVALAFWDRKLLFRPSEEDKEAHDVNPKATNL

Sequences:

>Translated_465_residues
MNQNTEQLRTEPIGRLLFKLSLPAMIGMLVTALYNIIDTIFVARGIGASAVAAIGIAFPIQMILMAVSSAVGLGGASISS
RKLGRGDDAGAAVTFLNVLVLVGLFAALAVSSAFFMLDDILKVFGATTEIMDLSRQYLSVVLISSPFFMFTLASSAMVRA
EGQAPYQMKVMLTTVLVNLVTTPLFIFTFDWGMYGAASSTALAQVIGSVLMLRFFFARKPKTELRFKWRQFRIRFDEMKE
IMAIGSSSFIMMVSQSILFVGVNVMLGTYGGTEELAIFAIINKFMALVGMPIMGIVQGMQPIVGYNYGAERFDRMRETIW
TAMRAGIAIAVIIWLILQLFPQTLMGWFSTDEALIAGGVTAIHILFAVSFLPSVQMLVNGIYQSLGKARVAMFLSLSRQT
LYTIPLVFILPSFLGVFGVWLAFPIADAMTFLTAVALAFWDRKLLFRPSEEDKEAHDVNPKATNL
>Mature_465_residues
MNQNTEQLRTEPIGRLLFKLSLPAMIGMLVTALYNIIDTIFVARGIGASAVAAIGIAFPIQMILMAVSSAVGLGGASISS
RKLGRGDDAGAAVTFLNVLVLVGLFAALAVSSAFFMLDDILKVFGATTEIMDLSRQYLSVVLISSPFFMFTLASSAMVRA
EGQAPYQMKVMLTTVLVNLVTTPLFIFTFDWGMYGAASSTALAQVIGSVLMLRFFFARKPKTELRFKWRQFRIRFDEMKE
IMAIGSSSFIMMVSQSILFVGVNVMLGTYGGTEELAIFAIINKFMALVGMPIMGIVQGMQPIVGYNYGAERFDRMRETIW
TAMRAGIAIAVIIWLILQLFPQTLMGWFSTDEALIAGGVTAIHILFAVSFLPSVQMLVNGIYQSLGKARVAMFLSLSRQT
LYTIPLVFILPSFLGVFGVWLAFPIADAMTFLTAVALAFWDRKLLFRPSEEDKEAHDVNPKATNL

Specific function: Multidrug resistance efflux protein [H]

COG id: COG0534

COG function: function code V; Na+-driven multidrug efflux pump

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the multi antimicrobial extrusion (MATE) (TC 2.A.66.1) family. MepA subfamily [H]

Homologues:

Organism=Escherichia coli, GI226510954, Length=467, Percent_Identity=24.1970021413276, Blast_Score=69, Evalue=9e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002528
- InterPro:   IPR015522 [H]

Pfam domain/function: PF01554 MatE [H]

EC number: NA

Molecular weight: Translated: 50993; Mature: 50993

Theoretical pI: Translated: 9.78; Mature: 9.78

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
6.0 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
6.0 %Met     (Mature Protein)
6.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHCCCCCCCCC
>Mature Secondary Structure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HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA