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The map label for this gene is topA [H]

Identifier: 229916207

GI number: 229916207

Start: 433508

End: 435634

Strand: Reverse

Name: topA [H]

Synonym: EAT1b_0476

Alternate gene names: 229916207

Gene position: 435634-433508 (Counterclockwise)

Preceding gene: 229916209

Following gene: 229916205

Centisome position: 14.52

GC content: 49.46

Gene sequence:

>2127_bases
ATGAGACTAATCATTGCGGAAAAGCCATCACAAGCTCAAAAGTTGGCTGCTCCCTATCCATCTAAAAAGCGAAAAGAAGA
GATTGAAATCTCACCTTGTCCCCGTTTCCCTGAGGGGGCGATTGTCGTATGGGCCGTCGGTCACCTTTGTGAACTTCAAG
AACCGTCGCACTATCGTCCGGAATGGAAGTCGTGGAAGTACGAGGCACTTCCGATCGTCCCAGAACGATTTGATTATCGT
ATTTCAAAAGGGAAGTCGAAACCGTTCTATGCCATCAAAAAATGGCTCCATGACCGCTCGATTACCGATGTCATCATTGC
ATCGGATGCCGAACGGGAAGGAGAAGCGATCGTTCGTCTGATCCTCCGACTCGCAGGTAACAAAAAACCGCTTGCTCGAC
TATGGATTTCGAGTCTGACGGAGCAAGCAGTCGATCGGGGATTCTCGCAACTGTTGCCAGGTGAACAGACGATTCCATAC
TACCATGAGGCGATGAGTCGCGCCTGTGCGGACTGGCTCGTTGGGATGAACGCCTCACGTGCCTATACGACATTGTTGAA
GACGATTGGGATTGAAGACGTCTTCTCGCTCGGTCGTGTACAAACCCCGACGCTTGCCCTAATCGTCAATCGCGAACGAG
AAATTAAACAATTCGTACCAGAACCATACTTTGAAGTCGAGGCGACACTTGCAAAAGGTCGAGGCACGTTCAAAGCGAAG
TATACGTTTGGAAAGACAACGAAGCTCAAAACGCGTGAGGAGGCCGATGCGGTCGTCAAACGTGCATCTGGAACCGCGAT
TGTGAAGACAATCGACAAGGAAGAAAAAATCGAGCAACCCCCATTTTGGTTCAGTCTATCCGGACTTCAAGCAGAGGCGG
GGAAACGATTCGGATTCGGGGCGAAAAAGACGCTTGATATCGCTCAAAAGTTATATACGAAAGGGTGGATCAGTTACCCT
CGGACCGATTCAGGATTTGTCACACCCGATGAGGCGACGCTATTTCCACAGACAAAGGCAAGACTGCTAAAGTCCGACGC
CTATGCAGGACTTCGTGACCTGTTGACTGAGGACCCGTCGAAAAATCGACGCTATGTCAATGCCAAGAAAGTCAGTGACC
ACTACGCGATCATCCCGACGGAGGCGTGTGGTGACGTCACTCGACTTGGTGGGGATGAGGCGAAGCTATATGATTTGATC
AATCGACGTTTCCTTGCGGCCTTTGCACCCGCAGCGATGCTCGAGAAGACAACGGTGGATCTGATTGATGGAGGCGACTT
GTATCGCGCAAAAGGAACCGTCGTTGTGAGTCCTGGCTATCGACAAGTCGTGGAGATGAAATCAAAAGATGTCGAGCTTC
CTGCGCTGACGGTCGGTGAAGAATTTAAAGAGAAGAAAGTTGAAGTGTTGTCGAAACAGACAGAGCCACCGAAGCGATAT
ACGGAGGGCGCGCTCATTATGGCGATGAAAGTCGCAGGGAAACAACTCGATGACGAAGAGCTGATTCATATTATGAAAGA
AGTAGAGGGACTCGGGACCGAGGCGACACGTGCCAACATCATCGATGGATTGAAAAAGCGAGGCTATGTCCAACTTCAGA
AAAAAGAGCTCGTTCCTACAGACAAAGGGCGCCTGCTGATCGATGTGCTCGGAGACAGTATTTTGGCCTCGCCCGTCATG
ACAGCGAAATGGGAGAAACGTCTCCATGAAATCGGTCAAGCATCGGCCTCCGCTGCTGAATTCATTGAACAAGCGAAGAA
GATGTCCGTTCATTTGGTTGAAGAAGCGAAATCGCGAGTAGAAGCGGCTAATCCTGAGGGCTATACGATTGAAGCGAAGC
GTTATGGAAAAAAAGGAACGACTCGACCGAAGGCATCATTTGGTATCTGTCCGAATTGTGGAAAAGGGCTCGTCGAACAT
CCGAAGTTCATCGGCTGTAGTGGATATCGTGAAGGATGTCGCTTCACCGTATCCAAACAGGTGCTCGGCGTCGGACTCGT
CAAAGATGAGTTGAAGCAAATGATCAATGGGGGGCAATCGAACGTCCATACGTTTAAAAAAGGAGACAAGACGTTCAATG
CCTCATTGTATTTAGAAAAAGGTACACTCCGATTTGAATTCAAATAA

Upstream 100 bases:

>100_bases
CGATGTGTCCATCTTCGACTTGATATAGTAAAATACATAGTAGAAGCTAGCCAATCATGTACGTCTTGCCATTATGTAGA
AGGCGAAGTAAGGAGTTCTT

Downstream 100 bases:

>100_bases
AAAAGGGTCACGCATTAGAGCGGCCCTTCTGTCTTGTCAGTCTTTTCTCTTTGCTTGTCGTCTTCAATGCGACGATTGAT
TTCGTCATAGTCGAGGACGA

Product: DNA topoisomerase III

Products: NA

Alternate protein names: DNA topoisomerase I; Omega-protein; Relaxing enzyme; Swivelase; Untwisting enzyme [H]

Number of amino acids: Translated: 708; Mature: 708

Protein sequence:

>708_residues
MRLIIAEKPSQAQKLAAPYPSKKRKEEIEISPCPRFPEGAIVVWAVGHLCELQEPSHYRPEWKSWKYEALPIVPERFDYR
ISKGKSKPFYAIKKWLHDRSITDVIIASDAEREGEAIVRLILRLAGNKKPLARLWISSLTEQAVDRGFSQLLPGEQTIPY
YHEAMSRACADWLVGMNASRAYTTLLKTIGIEDVFSLGRVQTPTLALIVNREREIKQFVPEPYFEVEATLAKGRGTFKAK
YTFGKTTKLKTREEADAVVKRASGTAIVKTIDKEEKIEQPPFWFSLSGLQAEAGKRFGFGAKKTLDIAQKLYTKGWISYP
RTDSGFVTPDEATLFPQTKARLLKSDAYAGLRDLLTEDPSKNRRYVNAKKVSDHYAIIPTEACGDVTRLGGDEAKLYDLI
NRRFLAAFAPAAMLEKTTVDLIDGGDLYRAKGTVVVSPGYRQVVEMKSKDVELPALTVGEEFKEKKVEVLSKQTEPPKRY
TEGALIMAMKVAGKQLDDEELIHIMKEVEGLGTEATRANIIDGLKKRGYVQLQKKELVPTDKGRLLIDVLGDSILASPVM
TAKWEKRLHEIGQASASAAEFIEQAKKMSVHLVEEAKSRVEAANPEGYTIEAKRYGKKGTTRPKASFGICPNCGKGLVEH
PKFIGCSGYREGCRFTVSKQVLGVGLVKDELKQMINGGQSNVHTFKKGDKTFNASLYLEKGTLRFEFK

Sequences:

>Translated_708_residues
MRLIIAEKPSQAQKLAAPYPSKKRKEEIEISPCPRFPEGAIVVWAVGHLCELQEPSHYRPEWKSWKYEALPIVPERFDYR
ISKGKSKPFYAIKKWLHDRSITDVIIASDAEREGEAIVRLILRLAGNKKPLARLWISSLTEQAVDRGFSQLLPGEQTIPY
YHEAMSRACADWLVGMNASRAYTTLLKTIGIEDVFSLGRVQTPTLALIVNREREIKQFVPEPYFEVEATLAKGRGTFKAK
YTFGKTTKLKTREEADAVVKRASGTAIVKTIDKEEKIEQPPFWFSLSGLQAEAGKRFGFGAKKTLDIAQKLYTKGWISYP
RTDSGFVTPDEATLFPQTKARLLKSDAYAGLRDLLTEDPSKNRRYVNAKKVSDHYAIIPTEACGDVTRLGGDEAKLYDLI
NRRFLAAFAPAAMLEKTTVDLIDGGDLYRAKGTVVVSPGYRQVVEMKSKDVELPALTVGEEFKEKKVEVLSKQTEPPKRY
TEGALIMAMKVAGKQLDDEELIHIMKEVEGLGTEATRANIIDGLKKRGYVQLQKKELVPTDKGRLLIDVLGDSILASPVM
TAKWEKRLHEIGQASASAAEFIEQAKKMSVHLVEEAKSRVEAANPEGYTIEAKRYGKKGTTRPKASFGICPNCGKGLVEH
PKFIGCSGYREGCRFTVSKQVLGVGLVKDELKQMINGGQSNVHTFKKGDKTFNASLYLEKGTLRFEFK
>Mature_708_residues
MRLIIAEKPSQAQKLAAPYPSKKRKEEIEISPCPRFPEGAIVVWAVGHLCELQEPSHYRPEWKSWKYEALPIVPERFDYR
ISKGKSKPFYAIKKWLHDRSITDVIIASDAEREGEAIVRLILRLAGNKKPLARLWISSLTEQAVDRGFSQLLPGEQTIPY
YHEAMSRACADWLVGMNASRAYTTLLKTIGIEDVFSLGRVQTPTLALIVNREREIKQFVPEPYFEVEATLAKGRGTFKAK
YTFGKTTKLKTREEADAVVKRASGTAIVKTIDKEEKIEQPPFWFSLSGLQAEAGKRFGFGAKKTLDIAQKLYTKGWISYP
RTDSGFVTPDEATLFPQTKARLLKSDAYAGLRDLLTEDPSKNRRYVNAKKVSDHYAIIPTEACGDVTRLGGDEAKLYDLI
NRRFLAAFAPAAMLEKTTVDLIDGGDLYRAKGTVVVSPGYRQVVEMKSKDVELPALTVGEEFKEKKVEVLSKQTEPPKRY
TEGALIMAMKVAGKQLDDEELIHIMKEVEGLGTEATRANIIDGLKKRGYVQLQKKELVPTDKGRLLIDVLGDSILASPVM
TAKWEKRLHEIGQASASAAEFIEQAKKMSVHLVEEAKSRVEAANPEGYTIEAKRYGKKGTTRPKASFGICPNCGKGLVEH
PKFIGCSGYREGCRFTVSKQVLGVGLVKDELKQMINGGQSNVHTFKKGDKTFNASLYLEKGTLRFEFK

Specific function: The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another [H]

COG id: COG0550

COG function: function code L; Topoisomerase IA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI4507635, Length=524, Percent_Identity=27.2900763358779, Blast_Score=149, Evalue=1e-35,
Organism=Homo sapiens, GI10835218, Length=648, Percent_Identity=27.3148148148148, Blast_Score=141, Evalue=2e-33,
Organism=Escherichia coli, GI1788061, Length=622, Percent_Identity=36.1736334405145, Blast_Score=337, Evalue=2e-93,
Organism=Escherichia coli, GI1787529, Length=636, Percent_Identity=27.0440251572327, Blast_Score=172, Evalue=6e-44,
Organism=Caenorhabditis elegans, GI17555378, Length=513, Percent_Identity=26.5107212475634, Blast_Score=134, Evalue=2e-31,
Organism=Caenorhabditis elegans, GI32563869, Length=675, Percent_Identity=24.1481481481481, Blast_Score=128, Evalue=9e-30,
Organism=Saccharomyces cerevisiae, GI6323263, Length=539, Percent_Identity=25.0463821892393, Blast_Score=104, Evalue=4e-23,
Organism=Drosophila melanogaster, GI24585251, Length=655, Percent_Identity=25.9541984732824, Blast_Score=133, Evalue=4e-31,
Organism=Drosophila melanogaster, GI24640096, Length=624, Percent_Identity=24.8397435897436, Blast_Score=123, Evalue=4e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003601
- InterPro:   IPR013497
- InterPro:   IPR013824
- InterPro:   IPR013825
- InterPro:   IPR000380
- InterPro:   IPR003602
- InterPro:   IPR013498
- InterPro:   IPR006171 [H]

Pfam domain/function: PF01131 Topoisom_bac; PF01751 Toprim; PF01396 zf-C4_Topoisom [H]

EC number: =5.99.1.2 [H]

Molecular weight: Translated: 79182; Mature: 79182

Theoretical pI: Translated: 9.79; Mature: 9.79

Prosite motif: PS00396 TOPOISOMERASE_I_PROK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLIIAEKPSQAQKLAAPYPSKKRKEEIEISPCPRFPEGAIVVWAVGHLCELQEPSHYRP
CEEEEECCCCHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEHHHHHCCCCCCCCCC
EWKSWKYEALPIVPERFDYRISKGKSKPFYAIKKWLHDRSITDVIIASDAEREGEAIVRL
CCCCCCEEEECCCCHHHCHHCCCCCCCCHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHH
ILRLAGNKKPLARLWISSLTEQAVDRGFSQLLPGEQTIPYYHEAMSRACADWLVGMNASR
HHHHHCCCCHHHHHHHHHHHHHHHHCCHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCHH
AYTTLLKTIGIEDVFSLGRVQTPTLALIVNREREIKQFVPEPYFEVEATLAKGRGTFKAK
HHHHHHHHHCHHHHHHCCCCCCCEEEEEECCHHHHHHHCCCCCHHHHHHHHCCCCCEEEE
YTFGKTTKLKTREEADAVVKRASGTAIVKTIDKEEKIEQPPFWFSLSGLQAEAGKRFGFG
EEECCCCCCCCHHHHHHHHHHCCCCEEEEECCHHHHCCCCCCEEEECCCCHHCCCCCCCC
AKKTLDIAQKLYTKGWISYPRTDSGFVTPDEATLFPQTKARLLKSDAYAGLRDLLTEDPS
CHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEECCHHHHHHHHHHHHHHHHHHHCCCCC
KNRRYVNAKKVSDHYAIIPTEACGDVTRLGGDEAKLYDLINRRFLAAFAPAAMLEKTTVD
CCCCEEEHEECCCCEEEEECHHCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCEEE
LIDGGDLYRAKGTVVVSPGYRQVVEMKSKDVELPALTVGEEFKEKKVEVLSKQTEPPKRY
EECCCCEEEECCCEEECCCHHHHHHHHCCCCCCCEEECCHHHHHHHHHHHHHCCCCCHHH
TEGALIMAMKVAGKQLDDEELIHIMKEVEGLGTEATRANIIDGLKKRGYVQLQKKELVPT
CCCCEEEEEEHHCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCEEEEHHHCCCC
DKGRLLIDVLGDSILASPVMTAKWEKRLHEIGQASASAAEFIEQAKKMSVHLVEEAKSRV
CCCCEEEEECCCHHHHCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHH
EAANPEGYTIEAKRYGKKGTTRPKASFGICPNCGKGLVEHPKFIGCSGYREGCRFTVSKQ
HCCCCCCEEEEHHHCCCCCCCCCCCCCCCCCCCCCCHHCCCCEECCCCCCCCCEEEEHHH
VLGVGLVKDELKQMINGGQSNVHTFKKGDKTFNASLYLEKGTLRFEFK
HHHHHHHHHHHHHHHCCCCCCCHHHHCCCCEEEEEEEEECCEEEEEEC
>Mature Secondary Structure
MRLIIAEKPSQAQKLAAPYPSKKRKEEIEISPCPRFPEGAIVVWAVGHLCELQEPSHYRP
CEEEEECCCCHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEHHHHHCCCCCCCCCC
EWKSWKYEALPIVPERFDYRISKGKSKPFYAIKKWLHDRSITDVIIASDAEREGEAIVRL
CCCCCCEEEECCCCHHHCHHCCCCCCCCHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHH
ILRLAGNKKPLARLWISSLTEQAVDRGFSQLLPGEQTIPYYHEAMSRACADWLVGMNASR
HHHHHCCCCHHHHHHHHHHHHHHHHCCHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCHH
AYTTLLKTIGIEDVFSLGRVQTPTLALIVNREREIKQFVPEPYFEVEATLAKGRGTFKAK
HHHHHHHHHCHHHHHHCCCCCCCEEEEEECCHHHHHHHCCCCCHHHHHHHHCCCCCEEEE
YTFGKTTKLKTREEADAVVKRASGTAIVKTIDKEEKIEQPPFWFSLSGLQAEAGKRFGFG
EEECCCCCCCCHHHHHHHHHHCCCCEEEEECCHHHHCCCCCCEEEECCCCHHCCCCCCCC
AKKTLDIAQKLYTKGWISYPRTDSGFVTPDEATLFPQTKARLLKSDAYAGLRDLLTEDPS
CHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEECCHHHHHHHHHHHHHHHHHHHCCCCC
KNRRYVNAKKVSDHYAIIPTEACGDVTRLGGDEAKLYDLINRRFLAAFAPAAMLEKTTVD
CCCCEEEHEECCCCEEEEECHHCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCEEE
LIDGGDLYRAKGTVVVSPGYRQVVEMKSKDVELPALTVGEEFKEKKVEVLSKQTEPPKRY
EECCCCEEEECCCEEECCCHHHHHHHHCCCCCCCEEECCHHHHHHHHHHHHHCCCCCHHH
TEGALIMAMKVAGKQLDDEELIHIMKEVEGLGTEATRANIIDGLKKRGYVQLQKKELVPT
CCCCEEEEEEHHCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCEEEEHHHCCCC
DKGRLLIDVLGDSILASPVMTAKWEKRLHEIGQASASAAEFIEQAKKMSVHLVEEAKSRV
CCCCEEEEECCCHHHHCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHH
EAANPEGYTIEAKRYGKKGTTRPKASFGICPNCGKGLVEHPKFIGCSGYREGCRFTVSKQ
HCCCCCCEEEEHHHCCCCCCCCCCCCCCCCCCCCCCHHCCCCEECCCCCCCCCEEEEHHH
VLGVGLVKDELKQMINGGQSNVHTFKKGDKTFNASLYLEKGTLRFEFK
HHHHHHHHHHHHHHHCCCCCCCHHHHCCCCEEEEEEEEECCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1329032 [H]