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The map label for this gene is menE [H]

Identifier: 229916081

GI number: 229916081

Start: 312759

End: 314303

Strand: Reverse

Name: menE [H]

Synonym: EAT1b_0350

Alternate gene names: 229916081

Gene position: 314303-312759 (Counterclockwise)

Preceding gene: 229916082

Following gene: 229916076

Centisome position: 10.48

GC content: 46.47

Gene sequence:

>1545_bases
ATGGTTAAGTTATTGAAAGTCTTGTCGCAGCTCCATTTTCTATCACCATCGAACCTAGGTCGTCTATTCGTCGCAATCCG
TCAAAATGGAATCAACTTGATGCTTCTTTTTACATTGACTGAAAGAGCAGGGAGTCGAAATACGGCTTTAGTTGATAGCC
GAGAAACAGTGACGTATCAAGAGCTAAAGTCACGCTGTGAACACCTTGCTCATCATTTGGCTGATCAGTATGGGGTTAAA
AAGGGACAAAAAGTGGCCTTTCTTTGTAAGAACCACGCGTCTTTCGTACAAGGGATGTTTGCGGCTTCACGACTCGGTGC
AGACATCTACATGCTTCATTCATCGATGAGTCAACTGCAGTTCAACCGACTAGTAGAAGAACAGCCATTCGATCTTTTGA
TTTACGATGATGAGTTTGAAAACTTAATTGAAAACTCGTCTTACGAACGTTCGACTCTTGTCAGCTATCATGAGGAAAAG
GTATCAATCAATAGTTTATCGGATTCGAAGCGACATAAAAAAGGAAAGCTTCCGGCCGCATCGAGCGGGAAACTCGTGCT
GTTGACCGGCGGTACTACCGGAAAACCGAAACAGGTGACTCATAGACCATCCCTCTTTCACTTTCTAGCTCCATTCACGG
CTTTATTGAACCAGTTGCAGTTGCCGTCATTCAACACTGCCTATATTGCCACCCCGATTTACCATGGCTACGGCATCGCC
ATCCTTTTATCTTTTCTAGCGCTAGGGAAACAGGTCGTCATTCAAGAGAGCTTTGATGTGCAGAGAGCCTGTGACCTCAT
TCGGCGACACAAGGTAGAAGTGGTCACGGTCGTCCCGCTCATGATCCACAAAATGTTACGACACAATCCTACATCACTTC
ATTCTCTCGCTTGTATCGCGTCCGGTGGTGCGAAGCTTCATCCCGTTTTAGTCCGTGAAGTCCATAACGAGTTGGGGAGC
GTCTTATACAATCTCTATGGGACGTCGGAAGCGGGCTTGAATCTGATCGCCACACCAGAAGATTTACATTATGATGCGAA
CACGCTTGGGAGAAGGATTCCGGGAGGGGACTTAAAGGTAGTGAAAGATGGAGCGGAAGTCGAGCCAGGCGCCATCGGGG
AATTTTGCATACGAAATAAGTGGTCCATGCAAAGCAGGAAGAACCGCTGGATTCAAACGGGTGATGTCGGATACCGGGAT
GAGAACGGCTATTATTTTTTGTGTGGACGAACAGACGACTTAATCATCTCGGCTGGAAACAATATTTATCCTGGGGAAGT
CGAGACGGTTTTGCGCGACCATCCGAATGTCGAGGACGCCGCCGTCATCGGGATAGAGGATATTTACGCGGGTCACATTT
TGAAGGCATATGTACAGGTGTATGATGAAGTATTGACAGAAGAAACACTAGTGAGCTGGTTGCGCGAACGAGTGACAACA
TATCAGATTCCTCGAGAAATCGTGTTTGTGAAGGAGTTGCCTTATACAGCGGTTGGAAAATTGGATAGGAAACGACTTGA
GATGATCAATCACTCGTGCAGATGA

Upstream 100 bases:

>100_bases
TGGTGGTTGTTCCTCGGTCAAGTAACGTCACTTGTTTTCAGAAGACCGATAGACTGGATGGTCCCTGCATTACTTAAGAG
AAAGGAGCGTGACAGTAGGA

Downstream 100 bases:

>100_bases
ATAGTTAAGAAAGAATCGAAACGCATTAACCACAGATAAATAGAGTGATTAATGTTTCACTTTGAATTGTCTAATCCGCC
ACATGATTGAAACGATAAAA

Product: AMP-dependent synthetase and ligase

Products: NA

Alternate protein names: OSB-CoA synthetase; o-succinylbenzoyl-CoA synthetase [H]

Number of amino acids: Translated: 514; Mature: 514

Protein sequence:

>514_residues
MVKLLKVLSQLHFLSPSNLGRLFVAIRQNGINLMLLFTLTERAGSRNTALVDSRETVTYQELKSRCEHLAHHLADQYGVK
KGQKVAFLCKNHASFVQGMFAASRLGADIYMLHSSMSQLQFNRLVEEQPFDLLIYDDEFENLIENSSYERSTLVSYHEEK
VSINSLSDSKRHKKGKLPAASSGKLVLLTGGTTGKPKQVTHRPSLFHFLAPFTALLNQLQLPSFNTAYIATPIYHGYGIA
ILLSFLALGKQVVIQESFDVQRACDLIRRHKVEVVTVVPLMIHKMLRHNPTSLHSLACIASGGAKLHPVLVREVHNELGS
VLYNLYGTSEAGLNLIATPEDLHYDANTLGRRIPGGDLKVVKDGAEVEPGAIGEFCIRNKWSMQSRKNRWIQTGDVGYRD
ENGYYFLCGRTDDLIISAGNNIYPGEVETVLRDHPNVEDAAVIGIEDIYAGHILKAYVQVYDEVLTEETLVSWLRERVTT
YQIPREIVFVKELPYTAVGKLDRKRLEMINHSCR

Sequences:

>Translated_514_residues
MVKLLKVLSQLHFLSPSNLGRLFVAIRQNGINLMLLFTLTERAGSRNTALVDSRETVTYQELKSRCEHLAHHLADQYGVK
KGQKVAFLCKNHASFVQGMFAASRLGADIYMLHSSMSQLQFNRLVEEQPFDLLIYDDEFENLIENSSYERSTLVSYHEEK
VSINSLSDSKRHKKGKLPAASSGKLVLLTGGTTGKPKQVTHRPSLFHFLAPFTALLNQLQLPSFNTAYIATPIYHGYGIA
ILLSFLALGKQVVIQESFDVQRACDLIRRHKVEVVTVVPLMIHKMLRHNPTSLHSLACIASGGAKLHPVLVREVHNELGS
VLYNLYGTSEAGLNLIATPEDLHYDANTLGRRIPGGDLKVVKDGAEVEPGAIGEFCIRNKWSMQSRKNRWIQTGDVGYRD
ENGYYFLCGRTDDLIISAGNNIYPGEVETVLRDHPNVEDAAVIGIEDIYAGHILKAYVQVYDEVLTEETLVSWLRERVTT
YQIPREIVFVKELPYTAVGKLDRKRLEMINHSCR
>Mature_514_residues
MVKLLKVLSQLHFLSPSNLGRLFVAIRQNGINLMLLFTLTERAGSRNTALVDSRETVTYQELKSRCEHLAHHLADQYGVK
KGQKVAFLCKNHASFVQGMFAASRLGADIYMLHSSMSQLQFNRLVEEQPFDLLIYDDEFENLIENSSYERSTLVSYHEEK
VSINSLSDSKRHKKGKLPAASSGKLVLLTGGTTGKPKQVTHRPSLFHFLAPFTALLNQLQLPSFNTAYIATPIYHGYGIA
ILLSFLALGKQVVIQESFDVQRACDLIRRHKVEVVTVVPLMIHKMLRHNPTSLHSLACIASGGAKLHPVLVREVHNELGS
VLYNLYGTSEAGLNLIATPEDLHYDANTLGRRIPGGDLKVVKDGAEVEPGAIGEFCIRNKWSMQSRKNRWIQTGDVGYRD
ENGYYFLCGRTDDLIISAGNNIYPGEVETVLRDHPNVEDAAVIGIEDIYAGHILKAYVQVYDEVLTEETLVSWLRERVTT
YQIPREIVFVKELPYTAVGKLDRKRLEMINHSCR

Specific function: Converts 2-succinylbenzoate (OSB) to 2-succinylbenzoyl- CoA (OSB-CoA) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Partially Membrane-Associated [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family. MenE subfamily [H]

Homologues:

Organism=Homo sapiens, GI187761345, Length=532, Percent_Identity=24.812030075188, Blast_Score=129, Evalue=8e-30,
Organism=Homo sapiens, GI187761343, Length=532, Percent_Identity=24.812030075188, Blast_Score=129, Evalue=8e-30,
Organism=Homo sapiens, GI156151445, Length=348, Percent_Identity=27.2988505747126, Blast_Score=125, Evalue=8e-29,
Organism=Homo sapiens, GI115511026, Length=353, Percent_Identity=28.6118980169972, Blast_Score=118, Evalue=1e-26,
Organism=Homo sapiens, GI38505220, Length=348, Percent_Identity=27.5862068965517, Blast_Score=116, Evalue=4e-26,
Organism=Homo sapiens, GI42544132, Length=494, Percent_Identity=22.0647773279352, Blast_Score=115, Evalue=1e-25,
Organism=Homo sapiens, GI157311624, Length=353, Percent_Identity=28.328611898017, Blast_Score=112, Evalue=9e-25,
Organism=Homo sapiens, GI157311622, Length=353, Percent_Identity=28.328611898017, Blast_Score=112, Evalue=9e-25,
Organism=Homo sapiens, GI58082049, Length=353, Percent_Identity=27.4787535410765, Blast_Score=109, Evalue=5e-24,
Organism=Homo sapiens, GI122937307, Length=255, Percent_Identity=30.9803921568627, Blast_Score=106, Evalue=5e-23,
Organism=Homo sapiens, GI28416953, Length=536, Percent_Identity=24.4402985074627, Blast_Score=97, Evalue=3e-20,
Organism=Homo sapiens, GI13375727, Length=541, Percent_Identity=22.181146025878, Blast_Score=84, Evalue=3e-16,
Organism=Homo sapiens, GI8923896, Length=556, Percent_Identity=22.3021582733813, Blast_Score=73, Evalue=5e-13,
Organism=Homo sapiens, GI253970489, Length=379, Percent_Identity=24.0105540897098, Blast_Score=70, Evalue=6e-12,
Organism=Escherichia coli, GI1788107, Length=514, Percent_Identity=27.2373540856031, Blast_Score=168, Evalue=7e-43,
Organism=Escherichia coli, GI1786810, Length=513, Percent_Identity=27.0955165692008, Blast_Score=125, Evalue=5e-30,
Organism=Escherichia coli, GI145693145, Length=529, Percent_Identity=24.952741020794, Blast_Score=112, Evalue=5e-26,
Organism=Escherichia coli, GI221142682, Length=469, Percent_Identity=21.3219616204691, Blast_Score=104, Evalue=1e-23,
Organism=Escherichia coli, GI1790505, Length=516, Percent_Identity=23.4496124031008, Blast_Score=102, Evalue=5e-23,
Organism=Escherichia coli, GI1786529, Length=386, Percent_Identity=24.8704663212435, Blast_Score=83, Evalue=5e-17,
Organism=Escherichia coli, GI1788595, Length=323, Percent_Identity=26.3157894736842, Blast_Score=82, Evalue=8e-17,
Organism=Escherichia coli, GI1786801, Length=512, Percent_Identity=23.6328125, Blast_Score=68, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17558820, Length=475, Percent_Identity=24.4210526315789, Blast_Score=131, Evalue=9e-31,
Organism=Caenorhabditis elegans, GI32563687, Length=476, Percent_Identity=25, Blast_Score=125, Evalue=6e-29,
Organism=Caenorhabditis elegans, GI17559526, Length=382, Percent_Identity=26.7015706806283, Blast_Score=122, Evalue=3e-28,
Organism=Caenorhabditis elegans, GI17557194, Length=509, Percent_Identity=23.3791748526523, Blast_Score=122, Evalue=3e-28,
Organism=Caenorhabditis elegans, GI17538037, Length=361, Percent_Identity=28.808864265928, Blast_Score=118, Evalue=6e-27,
Organism=Caenorhabditis elegans, GI71994703, Length=535, Percent_Identity=24.2990654205607, Blast_Score=118, Evalue=8e-27,
Organism=Caenorhabditis elegans, GI71994694, Length=535, Percent_Identity=24.2990654205607, Blast_Score=118, Evalue=9e-27,
Organism=Caenorhabditis elegans, GI71994690, Length=535, Percent_Identity=24.2990654205607, Blast_Score=117, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI71996755, Length=478, Percent_Identity=23.0125523012552, Blast_Score=110, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI17531443, Length=518, Percent_Identity=22.5868725868726, Blast_Score=109, Evalue=4e-24,
Organism=Caenorhabditis elegans, GI17560308, Length=354, Percent_Identity=27.1186440677966, Blast_Score=103, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI17560140, Length=301, Percent_Identity=26.2458471760797, Blast_Score=92, Evalue=7e-19,
Organism=Caenorhabditis elegans, GI25141359, Length=442, Percent_Identity=22.1719457013575, Blast_Score=89, Evalue=4e-18,
Organism=Caenorhabditis elegans, GI71985884, Length=354, Percent_Identity=23.728813559322, Blast_Score=77, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6319699, Length=421, Percent_Identity=26.8408551068884, Blast_Score=131, Evalue=3e-31,
Organism=Saccharomyces cerevisiae, GI6323182, Length=532, Percent_Identity=24.2481203007519, Blast_Score=101, Evalue=2e-22,
Organism=Saccharomyces cerevisiae, GI6319264, Length=535, Percent_Identity=23.7383177570093, Blast_Score=87, Evalue=6e-18,
Organism=Drosophila melanogaster, GI18859661, Length=387, Percent_Identity=28.6821705426357, Blast_Score=114, Evalue=2e-25,
Organism=Drosophila melanogaster, GI24581924, Length=363, Percent_Identity=26.1707988980716, Blast_Score=113, Evalue=3e-25,
Organism=Drosophila melanogaster, GI21355181, Length=506, Percent_Identity=24.703557312253, Blast_Score=110, Evalue=2e-24,
Organism=Drosophila melanogaster, GI21358303, Length=477, Percent_Identity=24.1090146750524, Blast_Score=107, Evalue=2e-23,
Organism=Drosophila melanogaster, GI281365686, Length=316, Percent_Identity=28.1645569620253, Blast_Score=99, Evalue=6e-21,
Organism=Drosophila melanogaster, GI24648253, Length=340, Percent_Identity=26.7647058823529, Blast_Score=95, Evalue=1e-19,
Organism=Drosophila melanogaster, GI24648255, Length=340, Percent_Identity=27.0588235294118, Blast_Score=94, Evalue=2e-19,
Organism=Drosophila melanogaster, GI24648260, Length=346, Percent_Identity=26.878612716763, Blast_Score=92, Evalue=8e-19,
Organism=Drosophila melanogaster, GI20130357, Length=395, Percent_Identity=25.3164556962025, Blast_Score=89, Evalue=7e-18,
Organism=Drosophila melanogaster, GI21356441, Length=483, Percent_Identity=22.7743271221532, Blast_Score=89, Evalue=7e-18,
Organism=Drosophila melanogaster, GI24656500, Length=503, Percent_Identity=23.6580516898608, Blast_Score=86, Evalue=6e-17,
Organism=Drosophila melanogaster, GI19922652, Length=353, Percent_Identity=25.4957507082153, Blast_Score=86, Evalue=7e-17,
Organism=Drosophila melanogaster, GI21356947, Length=341, Percent_Identity=24.633431085044, Blast_Score=85, Evalue=1e-16,
Organism=Drosophila melanogaster, GI21355225, Length=534, Percent_Identity=23.7827715355805, Blast_Score=83, Evalue=4e-16,
Organism=Drosophila melanogaster, GI62472339, Length=565, Percent_Identity=23.1858407079646, Blast_Score=82, Evalue=8e-16,
Organism=Drosophila melanogaster, GI24667955, Length=544, Percent_Identity=23.3455882352941, Blast_Score=80, Evalue=3e-15,
Organism=Drosophila melanogaster, GI24653035, Length=340, Percent_Identity=21.7647058823529, Blast_Score=78, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24648257, Length=347, Percent_Identity=25.0720461095101, Blast_Score=77, Evalue=3e-14,
Organism=Drosophila melanogaster, GI24667959, Length=369, Percent_Identity=24.1192411924119, Blast_Score=74, Evalue=3e-13,
Organism=Drosophila melanogaster, GI161076582, Length=276, Percent_Identity=20.6521739130435, Blast_Score=66, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873
- InterPro:   IPR010192
- InterPro:   IPR023015 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.26 [H]

Molecular weight: Translated: 57789; Mature: 57789

Theoretical pI: Translated: 8.10; Mature: 8.10

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVKLLKVLSQLHFLSPSNLGRLFVAIRQNGINLMLLFTLTERAGSRNTALVDSRETVTYQ
CHHHHHHHHHHHCCCCCCCCCEEEEEECCCCEEEEEEEEHHCCCCCCCEEECCCCHHHHH
ELKSRCEHLAHHLADQYGVKKGQKVAFLCKNHASFVQGMFAASRLGADIYMLHSSMSQLQ
HHHHHHHHHHHHHHHHHCCCCCCEEEEEEHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
FNRLVEEQPFDLLIYDDEFENLIENSSYERSTLVSYHEEKVSINSLSDSKRHKKGKLPAA
HHHHHHCCCCEEEEECCHHHHHHCCCCCCHHHHHHHHHHHEEECCCCCHHHHHCCCCCCC
SSGKLVLLTGGTTGKPKQVTHRPSLFHFLAPFTALLNQLQLPSFNTAYIATPIYHGYGIA
CCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEECCHHCCHHHH
ILLSFLALGKQVVIQESFDVQRACDLIRRHKVEVVTVVPLMIHKMLRHNPTSLHSLACIA
HHHHHHHCCCCEEEECCCCHHHHHHHHHHCCCCEEEHHHHHHHHHHHCCCCHHHHHHHHC
SGGAKLHPVLVREVHNELGSVLYNLYGTSEAGLNLIATPEDLHYDANTLGRRIPGGDLKV
CCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCHHCCCCHHHHCCCCCCCCEEE
VKDGAEVEPGAIGEFCIRNKWSMQSRKNRWIQTGDVGYRDENGYYFLCGRTDDLIISAGN
EECCCCCCCCCCHHHHHCCCCCHHHHHCCCEEECCCCEEECCCEEEEEECCCCEEEECCC
NIYPGEVETVLRDHPNVEDAAVIGIEDIYAGHILKAYVQVYDEVLTEETLVSWLRERVTT
CCCCCHHHHHHHCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YQIPREIVFVKELPYTAVGKLDRKRLEMINHSCR
HCCCHHEEEEECCCCHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MVKLLKVLSQLHFLSPSNLGRLFVAIRQNGINLMLLFTLTERAGSRNTALVDSRETVTYQ
CHHHHHHHHHHHCCCCCCCCCEEEEEECCCCEEEEEEEEHHCCCCCCCEEECCCCHHHHH
ELKSRCEHLAHHLADQYGVKKGQKVAFLCKNHASFVQGMFAASRLGADIYMLHSSMSQLQ
HHHHHHHHHHHHHHHHHCCCCCCEEEEEEHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
FNRLVEEQPFDLLIYDDEFENLIENSSYERSTLVSYHEEKVSINSLSDSKRHKKGKLPAA
HHHHHHCCCCEEEEECCHHHHHHCCCCCCHHHHHHHHHHHEEECCCCCHHHHHCCCCCCC
SSGKLVLLTGGTTGKPKQVTHRPSLFHFLAPFTALLNQLQLPSFNTAYIATPIYHGYGIA
CCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEECCHHCCHHHH
ILLSFLALGKQVVIQESFDVQRACDLIRRHKVEVVTVVPLMIHKMLRHNPTSLHSLACIA
HHHHHHHCCCCEEEECCCCHHHHHHHHHHCCCCEEEHHHHHHHHHHHCCCCHHHHHHHHC
SGGAKLHPVLVREVHNELGSVLYNLYGTSEAGLNLIATPEDLHYDANTLGRRIPGGDLKV
CCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCHHCCCCHHHHCCCCCCCCEEE
VKDGAEVEPGAIGEFCIRNKWSMQSRKNRWIQTGDVGYRDENGYYFLCGRTDDLIISAGN
EECCCCCCCCCCHHHHHCCCCCHHHHHCCCEEECCCCEEECCCEEEEEECCCCEEEECCC
NIYPGEVETVLRDHPNVEDAAVIGIEDIYAGHILKAYVQVYDEVLTEETLVSWLRERVTT
CCCCCHHHHHHHCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YQIPREIVFVKELPYTAVGKLDRKRLEMINHSCR
HCCCHHEEEEECCCCHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA