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The map label for this gene is gatA [H]

Identifier: 229916076

GI number: 229916076

Start: 309277

End: 310686

Strand: Reverse

Name: gatA [H]

Synonym: EAT1b_0345

Alternate gene names: 229916076

Gene position: 310686-309277 (Counterclockwise)

Preceding gene: 229916081

Following gene: 229916075

Centisome position: 10.36

GC content: 54.68

Gene sequence:

>1410_bases
ATGATGGAACAAATCCAATTACATGAACTGACGATTGAGCGTGCGCATCGTGGGTATAAAGAAGGAGAATTCACGGCGAG
AGACGTGACCGCCTACTATTTAGGACGGATTGCGACGTATAATGAACGGCTTCACGCCGTCATTCAAGTCAATCCGGACG
CCCTGTTCGAGGCAGAGGCAGCAGACAGGGCCTACCGAAAGGGGGTACGCAAACCGCTACTCGGCATTCCCGTCTTAATC
AAAGACAATATCGAGACGAACGGTCTGATGCGAACGACAGCGGGCGCTGCCGCGATGCGACATCATTTCGCCGCTCGAGA
TGCCGAGCTCGTCAAACGCTTGCGGGTTGACGGGGCCATCATCTTGGGGAAAGCGAACCTGTCCGAGTGGGCGAACTTTT
TAACGGAAGATATGCCGAACGGCTGGAGTGCCGTCGGTGGACAGACGATGAACCCATACGGCGACAAGTTAGATGTAGGG
GGATCAAGCTCAGGGTCGGCTTCAGCGGTCGCGGCAAACCTCACGCTTCTCGCAGTCGGGTCTGAGACGAGCGGTTCAAT
CGTCCATCCGAGTGTCCATAACAGCATCGTCGGGATCAAGCCGACCGTCGGATTGATCAGTCGCTCCGGTATCATCCCGA
TCTCACGTTCTCAAGATACGGCGGGACCGATGGCGCGTACGCTTCGTGATGCGGTCATCGCATTACAGACAATGTGTGGT
GAAGATTCGGCTGACCCTGCGACGTGGGAAACTCCAGTCGGACCGCCTTATGTGACATGTCTCGATGTGAGGCAAGCGGT
CGGGATGCGTGTCGGGTTCGTCAAACCAGACATTTCAGAAGAAGAAGTCGGATTGTATGAAGGGGCGCTCCGATTGCTAC
GGCAAGCCGAGGTCGAACTCGTCGAGGTCACGCTGCCGAAGCATGAGGCGCTAGAGCAGGCGGACATCCTGTTCGACGAA
TTCAAGCTCGGGGTCGAGGCGTATCTTGCCAATACGACCGTGCCGTTTAAGACACTTTCCGACTTGATCGAGTGGAACGC
GGAACATCCTGAATCGATTCCGCACGGCCAGACCATCTTTGAAGAAGCGAACGCCAAATCTGGAAAACTGACTGAAGCAA
ACTATCTCAACCGTCGCATCGAGGATGTCCGACTCGCGAAAACGGAAGGGATTGATGCATTGCTTGCGTCGCATAAACTG
GACGCACTCGTCTTGCCACATGACATGAACTATGACATGGCTGCAAAAGCGGGTCATCCGACGATTACAATACCCTATAA
ACGAAAAGAGAGCGGTGCCCCATTCGGTCTCTCGTTCACGGGGACGAGCTATGCGGAGCGTGATTTGATCCGAGTCGCAT
ATGCGTTCGAACAGTTCGTCACACGACCGACGCCTCCAGATTATTCGTAA

Upstream 100 bases:

>100_bases
TGTTTTTTCTTAAGTGTATCAAGAACACATCGACTTGGCGATACGAAAAGGAAATCAGACGTAGAGCACGAATAAAGGGA
GAGAGGTAAAGGAGGAATGC

Downstream 100 bases:

>100_bases
GTTGCACCCGTCTCTCACGTCCGCTACAATGGTACGTATGCGGATTAGAGAAAGGACAGATTACAATGAAATCATCTATA
TACGGGTTGACGTTCGATCA

Product: Amidase

Products: ADP; phosphate; Gln-tRNA(Gln); L-glutamate

Alternate protein names: Glu-ADT subunit A [H]

Number of amino acids: Translated: 469; Mature: 469

Protein sequence:

>469_residues
MMEQIQLHELTIERAHRGYKEGEFTARDVTAYYLGRIATYNERLHAVIQVNPDALFEAEAADRAYRKGVRKPLLGIPVLI
KDNIETNGLMRTTAGAAAMRHHFAARDAELVKRLRVDGAIILGKANLSEWANFLTEDMPNGWSAVGGQTMNPYGDKLDVG
GSSSGSASAVAANLTLLAVGSETSGSIVHPSVHNSIVGIKPTVGLISRSGIIPISRSQDTAGPMARTLRDAVIALQTMCG
EDSADPATWETPVGPPYVTCLDVRQAVGMRVGFVKPDISEEEVGLYEGALRLLRQAEVELVEVTLPKHEALEQADILFDE
FKLGVEAYLANTTVPFKTLSDLIEWNAEHPESIPHGQTIFEEANAKSGKLTEANYLNRRIEDVRLAKTEGIDALLASHKL
DALVLPHDMNYDMAAKAGHPTITIPYKRKESGAPFGLSFTGTSYAERDLIRVAYAFEQFVTRPTPPDYS

Sequences:

>Translated_469_residues
MMEQIQLHELTIERAHRGYKEGEFTARDVTAYYLGRIATYNERLHAVIQVNPDALFEAEAADRAYRKGVRKPLLGIPVLI
KDNIETNGLMRTTAGAAAMRHHFAARDAELVKRLRVDGAIILGKANLSEWANFLTEDMPNGWSAVGGQTMNPYGDKLDVG
GSSSGSASAVAANLTLLAVGSETSGSIVHPSVHNSIVGIKPTVGLISRSGIIPISRSQDTAGPMARTLRDAVIALQTMCG
EDSADPATWETPVGPPYVTCLDVRQAVGMRVGFVKPDISEEEVGLYEGALRLLRQAEVELVEVTLPKHEALEQADILFDE
FKLGVEAYLANTTVPFKTLSDLIEWNAEHPESIPHGQTIFEEANAKSGKLTEANYLNRRIEDVRLAKTEGIDALLASHKL
DALVLPHDMNYDMAAKAGHPTITIPYKRKESGAPFGLSFTGTSYAERDLIRVAYAFEQFVTRPTPPDYS
>Mature_469_residues
MMEQIQLHELTIERAHRGYKEGEFTARDVTAYYLGRIATYNERLHAVIQVNPDALFEAEAADRAYRKGVRKPLLGIPVLI
KDNIETNGLMRTTAGAAAMRHHFAARDAELVKRLRVDGAIILGKANLSEWANFLTEDMPNGWSAVGGQTMNPYGDKLDVG
GSSSGSASAVAANLTLLAVGSETSGSIVHPSVHNSIVGIKPTVGLISRSGIIPISRSQDTAGPMARTLRDAVIALQTMCG
EDSADPATWETPVGPPYVTCLDVRQAVGMRVGFVKPDISEEEVGLYEGALRLLRQAEVELVEVTLPKHEALEQADILFDE
FKLGVEAYLANTTVPFKTLSDLIEWNAEHPESIPHGQTIFEEANAKSGKLTEANYLNRRIEDVRLAKTEGIDALLASHKL
DALVLPHDMNYDMAAKAGHPTITIPYKRKESGAPFGLSFTGTSYAERDLIRVAYAFEQFVTRPTPPDYS

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI222831590, Length=492, Percent_Identity=27.4390243902439, Blast_Score=141, Evalue=1e-33,
Organism=Homo sapiens, GI195972892, Length=253, Percent_Identity=28.4584980237154, Blast_Score=98, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17537465, Length=488, Percent_Identity=25, Blast_Score=100, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI17543272, Length=436, Percent_Identity=24.3119266055046, Blast_Score=94, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17556264, Length=224, Percent_Identity=28.125, Blast_Score=85, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17538252, Length=255, Percent_Identity=25.8823529411765, Blast_Score=72, Evalue=4e-13,
Organism=Saccharomyces cerevisiae, GI6323950, Length=279, Percent_Identity=29.7491039426523, Blast_Score=91, Evalue=4e-19,
Organism=Saccharomyces cerevisiae, GI6319685, Length=171, Percent_Identity=37.4269005847953, Blast_Score=88, Evalue=3e-18,
Organism=Drosophila melanogaster, GI24648113, Length=504, Percent_Identity=28.3730158730159, Blast_Score=117, Evalue=1e-26,
Organism=Drosophila melanogaster, GI21356731, Length=246, Percent_Identity=31.3008130081301, Blast_Score=102, Evalue=5e-22,
Organism=Drosophila melanogaster, GI24644968, Length=240, Percent_Identity=30, Blast_Score=93, Evalue=4e-19,
Organism=Drosophila melanogaster, GI161078093, Length=501, Percent_Identity=24.750499001996, Blast_Score=92, Evalue=9e-19,
Organism=Drosophila melanogaster, GI24652985, Length=441, Percent_Identity=26.7573696145125, Blast_Score=92, Evalue=1e-18,
Organism=Drosophila melanogaster, GI19922090, Length=441, Percent_Identity=26.7573696145125, Blast_Score=92, Evalue=1e-18,
Organism=Drosophila melanogaster, GI24652981, Length=441, Percent_Identity=26.7573696145125, Blast_Score=92, Evalue=1e-18,
Organism=Drosophila melanogaster, GI24652983, Length=441, Percent_Identity=26.7573696145125, Blast_Score=92, Evalue=1e-18,
Organism=Drosophila melanogaster, GI24648435, Length=246, Percent_Identity=32.1138211382114, Blast_Score=89, Evalue=7e-18,
Organism=Drosophila melanogaster, GI24648437, Length=246, Percent_Identity=32.1138211382114, Blast_Score=89, Evalue=7e-18,
Organism=Drosophila melanogaster, GI45550774, Length=246, Percent_Identity=32.1138211382114, Blast_Score=89, Evalue=8e-18,
Organism=Drosophila melanogaster, GI24648441, Length=188, Percent_Identity=35.6382978723404, Blast_Score=81, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24648439, Length=188, Percent_Identity=35.6382978723404, Blast_Score=81, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: 6.3.5.7

Molecular weight: Translated: 51180; Mature: 51180

Theoretical pI: Translated: 5.35; Mature: 5.35

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMEQIQLHELTIERAHRGYKEGEFTARDVTAYYLGRIATYNERLHAVIQVNPDALFEAEA
CCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEEEECCHHHEECHH
ADRAYRKGVRKPLLGIPVLIKDNIETNGLMRTTAGAAAMRHHFAARDAELVKRLRVDGAI
HHHHHHHHHCCCHHCCCEEEECCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHCCCEE
ILGKANLSEWANFLTEDMPNGWSAVGGQTMNPYGDKLDVGGSSSGSASAVAANLTLLAVG
EEECCCHHHHHHHHHHHCCCCCHHCCCCCCCCCCCEEECCCCCCCCCCEEEEEEEEEEEC
SETSGSIVHPSVHNSIVGIKPTVGLISRSGIIPISRSQDTAGPMARTLRDAVIALQTMCG
CCCCCCEECCHHHCCEEECCCCHHHHCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHC
EDSADPATWETPVGPPYVTCLDVRQAVGMRVGFVKPDISEEEVGLYEGALRLLRQAEVEL
CCCCCCCCCCCCCCCCEEHHHHHHHHHCCEEEEECCCCCHHHHHHHHHHHHHHHHHCEEE
VEVTLPKHEALEQADILFDEFKLGVEAYLANTTVPFKTLSDLIEWNAEHPESIPHGQTIF
EEEECCHHHHHHHHHHHHHHHHHCHHHEEECCCCCHHHHHHHHHCCCCCCCCCCCCHHHH
EEANAKSGKLTEANYLNRRIEDVRLAKTEGIDALLASHKLDALVLPHDMNYDMAAKAGHP
HHCCCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCEEEECCCCCCCCHHCCCCC
TITIPYKRKESGAPFGLSFTGTSYAERDLIRVAYAFEQFVTRPTPPDYS
EEEEEECCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MMEQIQLHELTIERAHRGYKEGEFTARDVTAYYLGRIATYNERLHAVIQVNPDALFEAEA
CCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEEEECCHHHEECHH
ADRAYRKGVRKPLLGIPVLIKDNIETNGLMRTTAGAAAMRHHFAARDAELVKRLRVDGAI
HHHHHHHHHCCCHHCCCEEEECCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHCCCEE
ILGKANLSEWANFLTEDMPNGWSAVGGQTMNPYGDKLDVGGSSSGSASAVAANLTLLAVG
EEECCCHHHHHHHHHHHCCCCCHHCCCCCCCCCCCEEECCCCCCCCCCEEEEEEEEEEEC
SETSGSIVHPSVHNSIVGIKPTVGLISRSGIIPISRSQDTAGPMARTLRDAVIALQTMCG
CCCCCCEECCHHHCCEEECCCCHHHHCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHC
EDSADPATWETPVGPPYVTCLDVRQAVGMRVGFVKPDISEEEVGLYEGALRLLRQAEVEL
CCCCCCCCCCCCCCCCEEHHHHHHHHHCCEEEEECCCCCHHHHHHHHHHHHHHHHHCEEE
VEVTLPKHEALEQADILFDEFKLGVEAYLANTTVPFKTLSDLIEWNAEHPESIPHGQTIF
EEEECCHHHHHHHHHHHHHHHHHCHHHEEECCCCCHHHHHHHHHCCCCCCCCCCCCHHHH
EEANAKSGKLTEANYLNRRIEDVRLAKTEGIDALLASHKLDALVLPHDMNYDMAAKAGHP
HHCCCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCEEEECCCCCCCCHHCCCCC
TITIPYKRKESGAPFGLSFTGTSYAERDLIRVAYAFEQFVTRPTPPDYS
EEEEEECCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; Glu-tRNA(Gln); L-glutamine

Specific reaction: ATP + Glu-tRNA(Gln) + L-glutamine = ADP + phosphate + Gln-tRNA(Gln) + L-glutamate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA