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The map label for this gene is potE [H]

Identifier: 229586814

GI number: 229586814

Start: 710528

End: 711811

Strand: Direct

Name: potE [H]

Synonym: RAF_ORF0680

Alternate gene names: 229586814

Gene position: 710528-711811 (Clockwise)

Preceding gene: 229586812

Following gene: 229586815

Centisome position: 55.57

GC content: 35.75

Gene sequence:

>1284_bases
ATGTCACAAAAATTAGGTTTCTGGGCGGTTTTTGCCTTAGTAACCGGCAGTCAAATCGGTACTAGCGTTTTTATATTGCC
GTTAAGTTTAGCACCGTTCGGTATATACAGCATTTGGGGCTGGGTACTTTCATTATTCGGTGCTATGAGTATAGCACTTG
TATTTTCTACCCTTTGTGCAAAATTTCCTAAAACAGGAGGTCCGCACGTTTATGTACGGGAAAATTTCGGGGATAAAATA
GCTTTTTTCACCGGTTGGACTTACTGGGTTATATCCTTTGTCAGTACAAGTATAGTAGTCATTTCGGCAATAGGTTATCT
AACGCCTTTTTTTAAATCACAAGCGATTTTAGACTTAATATTACAGATCATATTATTAGGTGCTATTATGGTTTTAAATT
TAAAAGGTCCTGAAGTAGCAGGAAAAGCAGAGTTTTATTTAACTCTCTTAAAATTTGTTCCATTGCTTATAGTAGGTTTA
TGTGCTTTATCACATTTTAATATAGATAATATAACTATTGCCGAGGAAGTAGAGCATTTAAGCATTCCGAGCATTATGGG
AAGAGTTGTACTTCTTACTTTTTGGGGATTTATCGGCATAGAATGCGCAACTACCACGGCAGGTGCGGTAAAAGATCCGG
CAAAAACCATTCCACGAGCCATAATAGTTGGAACTTTTTGCGTAGCAGTGTTATATATTATTAATAGCATAGGTATAATG
GGATTAATCCCTGCTTCTGAACTTGTCAGTGCTAAAGCTCCTTATGCCGATGCCGCTTCATTATTATTCGGGGGTAAATG
GTCAAGCGTAATTACGGTTATAGCCTCGATTATATGTATAGGTACGCTCAATGCTTGGGTGCTAACTAGCGGACAGATAG
CTCTTGGACTTGCAGAAGACGGTTTATTACCGAAATTTTTTGCTAAGAAAAATAGCAATAATGCTCCAACTCACGGAATT
ATTGTAAGCTGCCTTGGCATTGTGCCGTTATTAGTCTTTACGGTAAATGATAATTTTGCCAAACAGATTACACAAATAAT
AGATTTCTCGGCAATCACATTCTTATTCGTCTATTTAATTTGTAGCTTAGCTTTTTTAAAGGTGATTTTTAGCTCAAAAG
AAAATTTTTCTTATTATTATTTACTTATAGCTATAATATCGATTATCTTTTGTGCATGGGTTATCTATGAAACACCTGTT
AAAACTCTGATTATAGCTAGTTCTTTTACTATCTTTGGTATTCCTTTGTATTATGGATGGTATAAATGTCATTCCCGTCT
ATGA

Upstream 100 bases:

>100_bases
TTGTTTAGAAATCAAGAGTAAAATAATTATAGTAGTTTTCACTATTGCTAAAAATCATTATTTCAGCTATGTTAAAGCTG
TTTCTTTAAATTAGATTAAT

Downstream 100 bases:

>100_bases
GCGTTGCCTAGCTGGATGCTGAGCTGTCATTGCGAGCGACTACAAGCAGCATGGCAATCCAGAAAAAATAATAAAAAAAT
TCTGTAAATCAGAATTTTTT

Product: Putrescine-ornithine antiporter

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 427; Mature: 426

Protein sequence:

>427_residues
MSQKLGFWAVFALVTGSQIGTSVFILPLSLAPFGIYSIWGWVLSLFGAMSIALVFSTLCAKFPKTGGPHVYVRENFGDKI
AFFTGWTYWVISFVSTSIVVISAIGYLTPFFKSQAILDLILQIILLGAIMVLNLKGPEVAGKAEFYLTLLKFVPLLIVGL
CALSHFNIDNITIAEEVEHLSIPSIMGRVVLLTFWGFIGIECATTTAGAVKDPAKTIPRAIIVGTFCVAVLYIINSIGIM
GLIPASELVSAKAPYADAASLLFGGKWSSVITVIASIICIGTLNAWVLTSGQIALGLAEDGLLPKFFAKKNSNNAPTHGI
IVSCLGIVPLLVFTVNDNFAKQITQIIDFSAITFLFVYLICSLAFLKVIFSSKENFSYYYLLIAIISIIFCAWVIYETPV
KTLIIASSFTIFGIPLYYGWYKCHSRL

Sequences:

>Translated_427_residues
MSQKLGFWAVFALVTGSQIGTSVFILPLSLAPFGIYSIWGWVLSLFGAMSIALVFSTLCAKFPKTGGPHVYVRENFGDKI
AFFTGWTYWVISFVSTSIVVISAIGYLTPFFKSQAILDLILQIILLGAIMVLNLKGPEVAGKAEFYLTLLKFVPLLIVGL
CALSHFNIDNITIAEEVEHLSIPSIMGRVVLLTFWGFIGIECATTTAGAVKDPAKTIPRAIIVGTFCVAVLYIINSIGIM
GLIPASELVSAKAPYADAASLLFGGKWSSVITVIASIICIGTLNAWVLTSGQIALGLAEDGLLPKFFAKKNSNNAPTHGI
IVSCLGIVPLLVFTVNDNFAKQITQIIDFSAITFLFVYLICSLAFLKVIFSSKENFSYYYLLIAIISIIFCAWVIYETPV
KTLIIASSFTIFGIPLYYGWYKCHSRL
>Mature_426_residues
SQKLGFWAVFALVTGSQIGTSVFILPLSLAPFGIYSIWGWVLSLFGAMSIALVFSTLCAKFPKTGGPHVYVRENFGDKIA
FFTGWTYWVISFVSTSIVVISAIGYLTPFFKSQAILDLILQIILLGAIMVLNLKGPEVAGKAEFYLTLLKFVPLLIVGLC
ALSHFNIDNITIAEEVEHLSIPSIMGRVVLLTFWGFIGIECATTTAGAVKDPAKTIPRAIIVGTFCVAVLYIINSIGIMG
LIPASELVSAKAPYADAASLLFGGKWSSVITVIASIICIGTLNAWVLTSGQIALGLAEDGLLPKFFAKKNSNNAPTHGII
VSCLGIVPLLVFTVNDNFAKQITQIIDFSAITFLFVYLICSLAFLKVIFSSKENFSYYYLLIAIISIIFCAWVIYETPVK
TLIIASSFTIFGIPLYYGWYKCHSRL

Specific function: Major component of the acid-resistance (AR) system allowing enteric pathogens to survive the acidic environment in the stomach. Exchanges extracellular arginine for its intracellular decarboxylation product agmatine (Agm) thereby expelling intracellular p

COG id: COG0531

COG function: function code E; Amino acid transporters

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amino acid-polyamine-organocation (APC) superfamily [H]

Homologues:

Organism=Homo sapiens, GI7657683, Length=293, Percent_Identity=26.6211604095563, Blast_Score=72, Evalue=7e-13,
Organism=Escherichia coli, GI2367353, Length=405, Percent_Identity=27.9012345679012, Blast_Score=193, Evalue=2e-50,
Organism=Escherichia coli, GI1786908, Length=424, Percent_Identity=24.0566037735849, Blast_Score=137, Evalue=1e-33,
Organism=Escherichia coli, GI1790575, Length=430, Percent_Identity=24.8837209302326, Blast_Score=134, Evalue=9e-33,
Organism=Escherichia coli, GI87082250, Length=454, Percent_Identity=25.5506607929515, Blast_Score=107, Evalue=2e-24,
Organism=Escherichia coli, GI1787890, Length=361, Percent_Identity=24.6537396121884, Blast_Score=105, Evalue=6e-24,
Organism=Escherichia coli, GI1790653, Length=309, Percent_Identity=26.8608414239482, Blast_Score=104, Evalue=1e-23,
Organism=Escherichia coli, GI1790584, Length=372, Percent_Identity=21.7741935483871, Blast_Score=79, Evalue=6e-16,
Organism=Escherichia coli, GI87081915, Length=402, Percent_Identity=23.8805970149254, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI1786602, Length=313, Percent_Identity=23.6421725239617, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI87081708, Length=299, Percent_Identity=24.0802675585284, Blast_Score=63, Evalue=4e-11,
Organism=Escherichia coli, GI1786302, Length=319, Percent_Identity=21.6300940438871, Blast_Score=62, Evalue=8e-11,
Organism=Caenorhabditis elegans, GI32566699, Length=354, Percent_Identity=24.8587570621469, Blast_Score=80, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI71995388, Length=349, Percent_Identity=23.7822349570201, Blast_Score=65, Evalue=5e-11,
Organism=Caenorhabditis elegans, GI71995382, Length=349, Percent_Identity=23.7822349570201, Blast_Score=65, Evalue=6e-11,
Organism=Drosophila melanogaster, GI21357367, Length=450, Percent_Identity=24, Blast_Score=73, Evalue=3e-13,
Organism=Drosophila melanogaster, GI45550968, Length=460, Percent_Identity=22.8260869565217, Blast_Score=73, Evalue=3e-13,
Organism=Drosophila melanogaster, GI19921172, Length=460, Percent_Identity=22.8260869565217, Blast_Score=73, Evalue=3e-13,
Organism=Drosophila melanogaster, GI221331183, Length=454, Percent_Identity=25.5506607929515, Blast_Score=67, Evalue=2e-11,
Organism=Drosophila melanogaster, GI17647653, Length=454, Percent_Identity=25.5506607929515, Blast_Score=67, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24664379, Length=454, Percent_Identity=25.5506607929515, Blast_Score=67, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004841
- InterPro:   IPR002293 [H]

Pfam domain/function: PF00324 AA_permease [H]

EC number: NA

Molecular weight: Translated: 46470; Mature: 46339

Theoretical pI: Translated: 8.45; Mature: 8.45

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQKLGFWAVFALVTGSQIGTSVFILPLSLAPFGIYSIWGWVLSLFGAMSIALVFSTLCA
CCCCHHHHHHHHHHHCCCCCCEEEEEEHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KFPKTGGPHVYVRENFGDKIAFFTGWTYWVISFVSTSIVVISAIGYLTPFFKSQAILDLI
HCCCCCCCEEEEECCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LQIILLGAIMVLNLKGPEVAGKAEFYLTLLKFVPLLIVGLCALSHFNIDNITIAEEVEHL
HHHHHHHHHHHEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEHHHHHHHC
SIPSIMGRVVLLTFWGFIGIECATTTAGAVKDPAKTIPRAIIVGTFCVAVLYIINSIGIM
CHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHH
GLIPASELVSAKAPYADAASLLFGGKWSSVITVIASIICIGTLNAWVLTSGQIALGLAED
EECCHHHHHHCCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHCCCHHEEECCCEEEEECCC
GLLPKFFAKKNSNNAPTHGIIVSCLGIVPLLVFTVNDNFAKQITQIIDFSAITFLFVYLI
CCCHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHH
CSLAFLKVIFSSKENFSYYYLLIAIISIIFCAWVIYETPVKTLIIASSFTIFGIPLYYGW
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHEECCHHHEEHHHHHHH
YKCHSRL
HHHHCCC
>Mature Secondary Structure 
SQKLGFWAVFALVTGSQIGTSVFILPLSLAPFGIYSIWGWVLSLFGAMSIALVFSTLCA
CCCHHHHHHHHHHHCCCCCCEEEEEEHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KFPKTGGPHVYVRENFGDKIAFFTGWTYWVISFVSTSIVVISAIGYLTPFFKSQAILDLI
HCCCCCCCEEEEECCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LQIILLGAIMVLNLKGPEVAGKAEFYLTLLKFVPLLIVGLCALSHFNIDNITIAEEVEHL
HHHHHHHHHHHEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEHHHHHHHC
SIPSIMGRVVLLTFWGFIGIECATTTAGAVKDPAKTIPRAIIVGTFCVAVLYIINSIGIM
CHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHH
GLIPASELVSAKAPYADAASLLFGGKWSSVITVIASIICIGTLNAWVLTSGQIALGLAED
EECCHHHHHHCCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHCCCHHEEECCCEEEEECCC
GLLPKFFAKKNSNNAPTHGIIVSCLGIVPLLVFTVNDNFAKQITQIIDFSAITFLFVYLI
CCCHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHH
CSLAFLKVIFSSKENFSYYYLLIAIISIIFCAWVIYETPVKTLIIASSFTIFGIPLYYGW
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHEECCHHHEEHHHHHHH
YKCHSRL
HHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11586360; 12142430 [H]