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The map label for this gene is prfA [H]

Identifier: 229586782

GI number: 229586782

Start: 673843

End: 674910

Strand: Direct

Name: prfA [H]

Synonym: RAF_ORF0640

Alternate gene names: 229586782

Gene position: 673843-674910 (Clockwise)

Preceding gene: 229586781

Following gene: 229586785

Centisome position: 52.7

GC content: 37.27

Gene sequence:

>1068_bases
ATGCGTTTTTCAGATAATTTAGCAAAAATTTTAGACAAATACGAAAATTTAGGCAACAAACTATCTTCCGGCATTATGGG
AGACGAGTTTGTTAAGGCATCTAAAGAATATGCAGAACTTGAAGATGTTGTAGCAAAAATTAAAGAATATAATAAGGCTA
AATCTGAGCTTGAAGAAGCAAATAATTTTAAGCTAGAAGTGGGACTTGATAATGCCACTTTAGAAATGATTGAAGACGAA
ATATACACTCTTGAAAATTCGTTACCAAAACTTGAAAGAGCCGTAAAAATTGCTTTATTACCAAAAGATGATGCGGATAG
TAAAAGTGCTATTATTGAAGTTAGAGCAGGAAGCGGCGGAGAAGAAGCGGCACTTTTTGCTGCCGTACTTTTTAATATGT
ATCAAAGATATGCTGAGTTAAAAGGATGGCGTTTTGAGATATTAGCAATTTCCGATACGGGAATAGGCGGTTATAAAGAA
GCATCGGCGTCAATAAAAGGCAAAGATGTATTCTCTAAGCTCAAATTTGAGTCAGGCGTTCATAGAGTACAGAGAGTACC
GGAAACGGAATCACAAGGACGTATTCATACTTCGGCAGCAACGGTTGCAGTACTTCCTGAGGCTGAAGAGGTTGATATTA
AAATTGAAGATAAAGACTTAAGAATCGATACTTATAGAGCTTCAGGGGCTGGTGGACAGCACGTTAATACTACTGATTCT
GCCGTACGTATAACTCATATCCCCACCGGTATTACCGTAGCCTTGCAAGATGAGAAGTCACAGCATAAAAATAAAGCAAA
GGCGTTAAAAATTCTACGTGCTAGAATTTATGAAGAAGAACGACGTAAAAAGGAACAAGAAAGAGCCGATAGCAGAAGAG
GGCAGGTAGGTTCGGGAAATCGTTCGGAACGGATACGTACTTATAATTTCCCACAGGGAAGAGTATCCGACCATAGAATA
AACTTAACACTTTATAAGATAGACGAAGTAGTTAAAAACGGACAATTAGATGAGTTTGTTGAAGCTTTAATTGCAGATGA
TGAAGCTAAAAAACTTTTGGAGATATAA

Upstream 100 bases:

>100_bases
CAATCTCGTCAAATATCCTGAGATTGCTTCATCGCAGCTTTCAGTTGCTTCTCGCAATAACGTTGAAATCTCACCCATAA
ACAAATCACAATAAAATAAC

Downstream 100 bases:

>100_bases
GTGTATATCGTCATTGTGAGTAGGCATTGTTGCGTGGATCAATTTCACCTCTGTTCACCCCGTGGCTTGACCACGGGATC
CAGTTAAAAATACTAACATT

Product: peptide chain release factor 1

Products: NA

Alternate protein names: RF-1 [H]

Number of amino acids: Translated: 355; Mature: 355

Protein sequence:

>355_residues
MRFSDNLAKILDKYENLGNKLSSGIMGDEFVKASKEYAELEDVVAKIKEYNKAKSELEEANNFKLEVGLDNATLEMIEDE
IYTLENSLPKLERAVKIALLPKDDADSKSAIIEVRAGSGGEEAALFAAVLFNMYQRYAELKGWRFEILAISDTGIGGYKE
ASASIKGKDVFSKLKFESGVHRVQRVPETESQGRIHTSAATVAVLPEAEEVDIKIEDKDLRIDTYRASGAGGQHVNTTDS
AVRITHIPTGITVALQDEKSQHKNKAKALKILRARIYEEERRKKEQERADSRRGQVGSGNRSERIRTYNFPQGRVSDHRI
NLTLYKIDEVVKNGQLDEFVEALIADDEAKKLLEI

Sequences:

>Translated_355_residues
MRFSDNLAKILDKYENLGNKLSSGIMGDEFVKASKEYAELEDVVAKIKEYNKAKSELEEANNFKLEVGLDNATLEMIEDE
IYTLENSLPKLERAVKIALLPKDDADSKSAIIEVRAGSGGEEAALFAAVLFNMYQRYAELKGWRFEILAISDTGIGGYKE
ASASIKGKDVFSKLKFESGVHRVQRVPETESQGRIHTSAATVAVLPEAEEVDIKIEDKDLRIDTYRASGAGGQHVNTTDS
AVRITHIPTGITVALQDEKSQHKNKAKALKILRARIYEEERRKKEQERADSRRGQVGSGNRSERIRTYNFPQGRVSDHRI
NLTLYKIDEVVKNGQLDEFVEALIADDEAKKLLEI
>Mature_355_residues
MRFSDNLAKILDKYENLGNKLSSGIMGDEFVKASKEYAELEDVVAKIKEYNKAKSELEEANNFKLEVGLDNATLEMIEDE
IYTLENSLPKLERAVKIALLPKDDADSKSAIIEVRAGSGGEEAALFAAVLFNMYQRYAELKGWRFEILAISDTGIGGYKE
ASASIKGKDVFSKLKFESGVHRVQRVPETESQGRIHTSAATVAVLPEAEEVDIKIEDKDLRIDTYRASGAGGQHVNTTDS
AVRITHIPTGITVALQDEKSQHKNKAKALKILRARIYEEERRKKEQERADSRRGQVGSGNRSERIRTYNFPQGRVSDHRI
NLTLYKIDEVVKNGQLDEFVEALIADDEAKKLLEI

Specific function: Peptide chain release factor 1 directs the termination of translation in response to the peptide chain termination codons UAG and UAA [H]

COG id: COG0216

COG function: function code J; Protein chain release factor A

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family [H]

Homologues:

Organism=Homo sapiens, GI166795303, Length=316, Percent_Identity=46.8354430379747, Blast_Score=289, Evalue=2e-78,
Organism=Homo sapiens, GI34577120, Length=359, Percent_Identity=40.3899721448468, Blast_Score=256, Evalue=3e-68,
Organism=Homo sapiens, GI166795305, Length=209, Percent_Identity=48.8038277511962, Blast_Score=206, Evalue=2e-53,
Organism=Escherichia coli, GI1787462, Length=325, Percent_Identity=55.0769230769231, Blast_Score=345, Evalue=3e-96,
Organism=Escherichia coli, GI2367172, Length=314, Percent_Identity=38.8535031847134, Blast_Score=194, Evalue=7e-51,
Organism=Caenorhabditis elegans, GI17542784, Length=261, Percent_Identity=42.1455938697318, Blast_Score=198, Evalue=4e-51,
Organism=Saccharomyces cerevisiae, GI6321295, Length=372, Percent_Identity=43.2795698924731, Blast_Score=247, Evalue=2e-66,
Organism=Drosophila melanogaster, GI19921226, Length=328, Percent_Identity=42.9878048780488, Blast_Score=251, Evalue=6e-67,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR004373 [H]

Pfam domain/function: PF03462 PCRF; PF00472 RF-1 [H]

EC number: NA

Molecular weight: Translated: 39764; Mature: 39764

Theoretical pI: Translated: 5.60; Mature: 5.60

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRFSDNLAKILDKYENLGNKLSSGIMGDEFVKASKEYAELEDVVAKIKEYNKAKSELEEA
CCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
NNFKLEVGLDNATLEMIEDEIYTLENSLPKLERAVKIALLPKDDADSKSAIIEVRAGSGG
CCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCCCCCEEEEEECCCCC
EEAALFAAVLFNMYQRYAELKGWRFEILAISDTGIGGYKEASASIKGKDVFSKLKFESGV
HHHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCCCHHCCCCCCHHHHHHHHHHHHHH
HRVQRVPETESQGRIHTSAATVAVLPEAEEVDIKIEDKDLRIDTYRASGAGGQHVNTTDS
HHHHCCCCCCCCCEEEECCEEEEECCCCCCEEEEEECCCEEEEEEECCCCCCCCCCCCCC
AVRITHIPTGITVALQDEKSQHKNKAKALKILRARIYEEERRKKEQERADSRRGQVGSGN
EEEEEECCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
RSERIRTYNFPQGRVSDHRINLTLYKIDEVVKNGQLDEFVEALIADDEAKKLLEI
CCCCEEEECCCCCCCCCCEEEEEEEEHHHHHHCCCHHHHHHHHHCCHHHHHHHCC
>Mature Secondary Structure
MRFSDNLAKILDKYENLGNKLSSGIMGDEFVKASKEYAELEDVVAKIKEYNKAKSELEEA
CCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
NNFKLEVGLDNATLEMIEDEIYTLENSLPKLERAVKIALLPKDDADSKSAIIEVRAGSGG
CCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCCCCCEEEEEECCCCC
EEAALFAAVLFNMYQRYAELKGWRFEILAISDTGIGGYKEASASIKGKDVFSKLKFESGV
HHHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCCCHHCCCCCCHHHHHHHHHHHHHH
HRVQRVPETESQGRIHTSAATVAVLPEAEEVDIKIEDKDLRIDTYRASGAGGQHVNTTDS
HHHHCCCCCCCCCEEEECCEEEEECCCCCCEEEEEECCCEEEEEEECCCCCCCCCCCCCC
AVRITHIPTGITVALQDEKSQHKNKAKALKILRARIYEEERRKKEQERADSRRGQVGSGN
EEEEEECCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
RSERIRTYNFPQGRVSDHRINLTLYKIDEVVKNGQLDEFVEALIADDEAKKLLEI
CCCCEEEECCCCCCCCCCEEEEEEEEHHHHHHCCCHHHHHHHHHCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA