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The map label for this gene is pdhC [H]

Identifier: 229586781

GI number: 229586781

Start: 672472

End: 673710

Strand: Direct

Name: pdhC [H]

Synonym: RAF_ORF0639

Alternate gene names: 229586781

Gene position: 672472-673710 (Clockwise)

Preceding gene: 229586780

Following gene: 229586782

Centisome position: 52.6

GC content: 35.51

Gene sequence:

>1239_bases
ATGCCGATTAAAATTTTAATGCCTGCTTTATCTCCAACTATGACGGAAGGAAATCTAGCTAGGTGGTTAAAAAAAGAAGG
GGACAAAGTTAATCCTGGAGAAGTAATTGCTGAAATTGAAACCGATAAAGCAACGATGGAAGTAGAAGCAGTAGATGAAG
GTATACTTGCCAAAATAGTTATCCCACAAAATAGTCAAAATGTACCTGTTAACTCTTTAATTGCCGTGTTAAGTGAAGAG
GGAGAAGAAAAAACAGACATTGATGCGTTTATTGCAAAAAATAATAGTGTATCACCGTCACCGAAAACAGATGCTAATCT
TCCGAAACCCCACGAGAATATAGCTAATGTAGAAGAACAGGTAACAGTTATAAAACATGATGCTAGTAAAATATTCGCCT
CACCGCTTGCAAAAAGACTTGCAAAAATGAGAAATATTAGATTTGAGAGCGTGAAAGGTAGCGGTCCGCACGGTAGAATA
GTCAAACAAGATATCTTATCATATACTCCTAGCACTGCTCATAATAAAATAGTTAGCAGGAATCCTGAAGAATATCGTTT
AGTACCAAATAATAATATCCGCAAGATTATAGCCAAGCGTCTGCTTGAATCTAAACAAACAGTTCCACATTTTTATTTAT
CTATAGAATGTAATGTCGATAAATTGTTAGATATAAGAGAAGACATTAATAAATTTTTTTCTGAAGATAAATCAACAAGG
ATATCGGTTAATGATTTTATTATTTTAGCAGTAGCCAAAGCTTTACAAGAAGTGCCAAATGCCAATGCTAGCTGGGGAGA
AGATGCAATTAGATATTACAATAATGTCGATATTTCAGTAGCGGTAGCGATTGAGAACGGGCTTGTTACACCGATAGTTA
AAAATGCCAATCAAAAAAATATTCTAGAACTATCTCGTGAAATGAAAGCATTAATAAAGAAAGCAAAAGATAATAAATTA
ACCCCTGAAGAATTTCAGGGCGGAGGGTTTACCATTTCTAACCTCGGTATGTACGGTATTAAGAATTTCAATGCTATAAT
TAACCCACCACAAAGTTGTATAATGGGCGTCGGAGCTAGTGCAAAACGTGCTATAGTCAAGAATGATCAAATAACTATAG
CAACAATTATGGACGTAACTCTTTCTGCCGATCACCGAGTAGTAGACGGAGCAGTCGGTGCTGAGTTCTTGGCAGCATTT
AAAAAGTTTATAGAGAGTCCCGTTTTGATGTTGATATAA

Upstream 100 bases:

>100_bases
GTGTGGCAATCTCAGGAATTTTACTGTTTCATGAGGTTGCCGCGTCGAGGCTTGGCCTCTCCTCGCAATGACATGTAATA
ACAACAATAAAAAGTCAAAT

Downstream 100 bases:

>100_bases
GTCGTCATTACGAGCAGCCATAGGCTGCGTGACAATCTCGTCAAATATCCTGAGATTGCTTCATCGCAGCTTTCAGTTGC
TTCTCGCAATAACGTTGAAA

Product: branched-chain alpha-keto acid dehydrogenase subunit E2

Products: NA

Alternate protein names: Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex; E2 [H]

Number of amino acids: Translated: 412; Mature: 411

Protein sequence:

>412_residues
MPIKILMPALSPTMTEGNLARWLKKEGDKVNPGEVIAEIETDKATMEVEAVDEGILAKIVIPQNSQNVPVNSLIAVLSEE
GEEKTDIDAFIAKNNSVSPSPKTDANLPKPHENIANVEEQVTVIKHDASKIFASPLAKRLAKMRNIRFESVKGSGPHGRI
VKQDILSYTPSTAHNKIVSRNPEEYRLVPNNNIRKIIAKRLLESKQTVPHFYLSIECNVDKLLDIREDINKFFSEDKSTR
ISVNDFIILAVAKALQEVPNANASWGEDAIRYYNNVDISVAVAIENGLVTPIVKNANQKNILELSREMKALIKKAKDNKL
TPEEFQGGGFTISNLGMYGIKNFNAIINPPQSCIMGVGASAKRAIVKNDQITIATIMDVTLSADHRVVDGAVGAEFLAAF
KKFIESPVLMLI

Sequences:

>Translated_412_residues
MPIKILMPALSPTMTEGNLARWLKKEGDKVNPGEVIAEIETDKATMEVEAVDEGILAKIVIPQNSQNVPVNSLIAVLSEE
GEEKTDIDAFIAKNNSVSPSPKTDANLPKPHENIANVEEQVTVIKHDASKIFASPLAKRLAKMRNIRFESVKGSGPHGRI
VKQDILSYTPSTAHNKIVSRNPEEYRLVPNNNIRKIIAKRLLESKQTVPHFYLSIECNVDKLLDIREDINKFFSEDKSTR
ISVNDFIILAVAKALQEVPNANASWGEDAIRYYNNVDISVAVAIENGLVTPIVKNANQKNILELSREMKALIKKAKDNKL
TPEEFQGGGFTISNLGMYGIKNFNAIINPPQSCIMGVGASAKRAIVKNDQITIATIMDVTLSADHRVVDGAVGAEFLAAF
KKFIESPVLMLI
>Mature_411_residues
PIKILMPALSPTMTEGNLARWLKKEGDKVNPGEVIAEIETDKATMEVEAVDEGILAKIVIPQNSQNVPVNSLIAVLSEEG
EEKTDIDAFIAKNNSVSPSPKTDANLPKPHENIANVEEQVTVIKHDASKIFASPLAKRLAKMRNIRFESVKGSGPHGRIV
KQDILSYTPSTAHNKIVSRNPEEYRLVPNNNIRKIIAKRLLESKQTVPHFYLSIECNVDKLLDIREDINKFFSEDKSTRI
SVNDFIILAVAKALQEVPNANASWGEDAIRYYNNVDISVAVAIENGLVTPIVKNANQKNILELSREMKALIKKAKDNKLT
PEEFQGGGFTISNLGMYGIKNFNAIINPPQSCIMGVGASAKRAIVKNDQITIATIMDVTLSADHRVVDGAVGAEFLAAFK
KFIESPVLMLI

Specific function: The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components:pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydroge

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI31711992, Length=441, Percent_Identity=42.8571428571429, Blast_Score=348, Evalue=5e-96,
Organism=Homo sapiens, GI203098816, Length=460, Percent_Identity=38.695652173913, Blast_Score=292, Evalue=3e-79,
Organism=Homo sapiens, GI203098753, Length=460, Percent_Identity=38.695652173913, Blast_Score=292, Evalue=5e-79,
Organism=Homo sapiens, GI260898739, Length=167, Percent_Identity=45.5089820359281, Blast_Score=145, Evalue=5e-35,
Organism=Homo sapiens, GI110671329, Length=428, Percent_Identity=27.803738317757, Blast_Score=143, Evalue=3e-34,
Organism=Homo sapiens, GI19923748, Length=224, Percent_Identity=33.0357142857143, Blast_Score=126, Evalue=3e-29,
Organism=Escherichia coli, GI1786946, Length=425, Percent_Identity=32, Blast_Score=204, Evalue=7e-54,
Organism=Escherichia coli, GI1786305, Length=395, Percent_Identity=30.8860759493671, Blast_Score=163, Evalue=2e-41,
Organism=Caenorhabditis elegans, GI17560088, Length=434, Percent_Identity=41.0138248847926, Blast_Score=312, Evalue=2e-85,
Organism=Caenorhabditis elegans, GI17538894, Length=307, Percent_Identity=34.8534201954397, Blast_Score=180, Evalue=1e-45,
Organism=Caenorhabditis elegans, GI25146366, Length=424, Percent_Identity=31.6037735849057, Blast_Score=171, Evalue=9e-43,
Organism=Caenorhabditis elegans, GI17537937, Length=434, Percent_Identity=27.1889400921659, Blast_Score=157, Evalue=7e-39,
Organism=Saccharomyces cerevisiae, GI6324258, Length=457, Percent_Identity=39.8249452954048, Blast_Score=304, Evalue=2e-83,
Organism=Saccharomyces cerevisiae, GI6320352, Length=409, Percent_Identity=30.8068459657702, Blast_Score=162, Evalue=9e-41,
Organism=Saccharomyces cerevisiae, GI6321632, Length=190, Percent_Identity=31.5789473684211, Blast_Score=72, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24582497, Length=292, Percent_Identity=46.2328767123288, Blast_Score=275, Evalue=3e-74,
Organism=Drosophila melanogaster, GI20129315, Length=292, Percent_Identity=46.2328767123288, Blast_Score=275, Evalue=4e-74,
Organism=Drosophila melanogaster, GI18859875, Length=424, Percent_Identity=26.6509433962264, Blast_Score=150, Evalue=2e-36,
Organism=Drosophila melanogaster, GI24645909, Length=242, Percent_Identity=31.8181818181818, Blast_Score=124, Evalue=2e-28,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3096 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR006257
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.12 [H]

Molecular weight: Translated: 45343; Mature: 45212

Theoretical pI: Translated: 7.66; Mature: 7.66

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPIKILMPALSPTMTEGNLARWLKKEGDKVNPGEVIAEIETDKATMEVEAVDEGILAKIV
CCCEEEECCCCCCCCCCHHHHHHHHCCCCCCCCCEEEEEECCCCEEEEEECCCCCEEEEE
IPQNSQNVPVNSLIAVLSEEGEEKTDIDAFIAKNNSVSPSPKTDANLPKPHENIANVEEQ
ECCCCCCCCHHHHHHHHHHCCCCCCCCHHEEECCCCCCCCCCCCCCCCCCCHHHHHHHHH
VTVIKHDASKIFASPLAKRLAKMRNIRFESVKGSGPHGRIVKQDILSYTPSTAHNKIVSR
HHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCCCCHHHHHHHHCCCCHHHCHHHCC
NPEEYRLVPNNNIRKIIAKRLLESKQTVPHFYLSIECNVDKLLDIREDINKFFSEDKSTR
CCCCEEECCCCCHHHHHHHHHHHHCCCCCEEEEEEEECHHHHHHHHHHHHHHHCCCCCCE
ISVNDFIILAVAKALQEVPNANASWGEDAIRYYNNVDISVAVAIENGLVTPIVKNANQKN
EEHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHCCCCEEEEEEECCCCCCHHHCCCCHHH
ILELSREMKALIKKAKDNKLTPEEFQGGGFTISNLGMYGIKNFNAIINPPQSCIMGVGAS
HHHHHHHHHHHHHHHCCCCCCHHHHCCCCEEEECCCCCCCCCCCCCCCCHHHHHHCCCCC
AKRAIVKNDQITIATIMDVTLSADHRVVDGAVGAEFLAAFKKFIESPVLMLI
CCHHEECCCCEEEEEEEEEEECCCCEEECHHHHHHHHHHHHHHHCCCCEEEC
>Mature Secondary Structure 
PIKILMPALSPTMTEGNLARWLKKEGDKVNPGEVIAEIETDKATMEVEAVDEGILAKIV
CCEEEECCCCCCCCCCHHHHHHHHCCCCCCCCCEEEEEECCCCEEEEEECCCCCEEEEE
IPQNSQNVPVNSLIAVLSEEGEEKTDIDAFIAKNNSVSPSPKTDANLPKPHENIANVEEQ
ECCCCCCCCHHHHHHHHHHCCCCCCCCHHEEECCCCCCCCCCCCCCCCCCCHHHHHHHHH
VTVIKHDASKIFASPLAKRLAKMRNIRFESVKGSGPHGRIVKQDILSYTPSTAHNKIVSR
HHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCCCCHHHHHHHHCCCCHHHCHHHCC
NPEEYRLVPNNNIRKIIAKRLLESKQTVPHFYLSIECNVDKLLDIREDINKFFSEDKSTR
CCCCEEECCCCCHHHHHHHHHHHHCCCCCEEEEEEEECHHHHHHHHHHHHHHHCCCCCCE
ISVNDFIILAVAKALQEVPNANASWGEDAIRYYNNVDISVAVAIENGLVTPIVKNANQKN
EEHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHCCCCEEEEEEECCCCCCHHHCCCCHHH
ILELSREMKALIKKAKDNKLTPEEFQGGGFTISNLGMYGIKNFNAIINPPQSCIMGVGAS
HHHHHHHHHHHHHHHCCCCCCHHHHCCCCEEEECCCCCCCCCCCCCCCCHHHHHHCCCCC
AKRAIVKNDQITIATIMDVTLSADHRVVDGAVGAEFLAAFKKFIESPVLMLI
CCHHEECCCCEEEEEEEEEEECCCCEEECHHHHHHHHHHHHHHHCCCCEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11557893 [H]