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The map label for this gene is recJ [H]

Identifier: 229586785

GI number: 229586785

Start: 676966

End: 678729

Strand: Direct

Name: recJ [H]

Synonym: RAF_ORF0643

Alternate gene names: 229586785

Gene position: 676966-678729 (Clockwise)

Preceding gene: 229586782

Following gene: 229586786

Centisome position: 52.95

GC content: 33.28

Gene sequence:

>1764_bases
ATGAAACCACAAGCATCTATAAACGGTAAAATCTGGCAGCAGAAACTGATTAACGAAGATATAGTCACCGAGTTATGTCG
TAGTTTCAAAATTAGTGATTTACTTGCTAGGATAGTATCATTAAGAGTAAATAATCTAGAGGAAGTAGAAAATTTCTTGA
GACCGAAAATCAAAAATTTATTACCTAATCCTTTTCATCTACTTGATATGGAAAAGGCTGTAGATAGAACTGTTACAGCT
ATTTTAAATAACCAAAAAATATGTATCTTTGCCGATTATGATGTAGACGGTGCTACTTCAGCCGCTTTACTTAAAAATAT
TTTTAGAGACTTAAATATACTATGTGATATTTATGTTCCTGATCGTATAGCTGAAGGATACGGTCCAACTCCTTTTGCTA
TGCAAAAAATTAAAGATAACGGTACAGAATTATTAATTACGGTTGATTGCGGTGCTATGGCACATGAAGCCCTAAAATAC
GCAAAAGACGTTAACCTTGATGTTATAGTAATCGATCATCATATAGCTACGGAAATATTACCGGATGCAGTAGCTATAGT
AAATCCAAATCGTATTGATGAAAAAAGCGAATATAAACATTTGGCAGCCGTAGGAGTTGCTTTCTTATTTGCAACTGCAA
TATTATCGAATTTAAAGAAGCAAAATTATTTCACGCAAACAATGCTTCAGCCTAATCTAATGCAGTATCTAGATTTAGTG
GCTCTTGGTACTGTTTGCGATATGATGAAATTAACAGGCTTAAATCGCGCATTTGTTGCAAGCGGATTGAAAGTAATGCA
ACAAAGGCAAAATATAGGGATTAAAACATTATATGATATGGCAGGGCTTCATGAAATACCAAAATGTTATCATTTAGGAT
TTATTCTAGGTCCGCGTATCAATGCAGGCGGTAGAGTAGGAAAGTCAAGTTTAGGTGCTCATCTCCTATCTACTAGTTGT
TCTAATGAAGCAACTAAATTAGCTAAAGAACTTGAAAAACACAATAATGAGCGTAAAGTAATTGAGTTATTAATGATAGA
AGAAGCGATGGAAATTGCTTTGACACAACAAGATAGTAGTTTATTATTTATCGTAAAAGAAGGATGGCATCCAGGCGTTA
TAGGAATCGTTGCCGGCAAATTAAAAGAAAAATTTGATAAGCCGGTGGCAGTTATAGCCTTAAATGACGGGATTGGTAAA
GCTTCATGCCGTTCTATTTTAGGTATTGATTTTGGTGCTGAAATTATCAATGCTAAAAGCAAAGATTTGCTAATAGCAGG
CGGAGGTCATGCTATGGCGGCAGGTTTTACCGTAACGGCTGAAAAATTACAAGAATTGCAGAATTTTTTTAATAATGCTT
TTAGAATTAGTATAACTAAACTAGAAAATTATAAGCATGCGGAATATGATATTGATTTAACGGTTAATAGTGTGAATTTC
CCTTTAATGGAGGAATTAAACACTTTAGAGCCTTTCGGTCAAGGAAATCATGAGCCTATATTTAAATTTGATGATTTATT
TGTATTAAAAGCGGATATCGTTGGAAGTAAGCATATTAAATGTATGCTTGTTCCAAATAAGCGAGGCTTTGGTAATAAAG
CCTTATCTGCTATTGCTTTTAATTCGGTAGGAACTGCATTTGAAGATGTTTTATTAAGCCCTAAAGCAAAAAATATTGCC
GCAATAGGAACGTTAAAAACTAATAATTGGCAAGATAATTATACTATTCAATTAATTATAAAAGATATACTTCTAAAAGA
GTAG

Upstream 100 bases:

>100_bases
CCCGTGGTCAAGCCACGGGGTGAACAGAGGTGAAATTGATCCACGCAACAATGCCTACTCACAATGACGATATACAGCAA
AAATACAGCCTAATAAACAA

Downstream 100 bases:

>100_bases
ATAGACTAAGTATATATAATAACAACTTAAAAAATAATAATTTATGAGAAATTTAAAGAAAACAAAAAATACTACAATTT
CTAAAGACTTAGCAGAAGTA

Product: Single-stranded-DNA-specific exonuclease RecJ

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 587; Mature: 587

Protein sequence:

>587_residues
MKPQASINGKIWQQKLINEDIVTELCRSFKISDLLARIVSLRVNNLEEVENFLRPKIKNLLPNPFHLLDMEKAVDRTVTA
ILNNQKICIFADYDVDGATSAALLKNIFRDLNILCDIYVPDRIAEGYGPTPFAMQKIKDNGTELLITVDCGAMAHEALKY
AKDVNLDVIVIDHHIATEILPDAVAIVNPNRIDEKSEYKHLAAVGVAFLFATAILSNLKKQNYFTQTMLQPNLMQYLDLV
ALGTVCDMMKLTGLNRAFVASGLKVMQQRQNIGIKTLYDMAGLHEIPKCYHLGFILGPRINAGGRVGKSSLGAHLLSTSC
SNEATKLAKELEKHNNERKVIELLMIEEAMEIALTQQDSSLLFIVKEGWHPGVIGIVAGKLKEKFDKPVAVIALNDGIGK
ASCRSILGIDFGAEIINAKSKDLLIAGGGHAMAAGFTVTAEKLQELQNFFNNAFRISITKLENYKHAEYDIDLTVNSVNF
PLMEELNTLEPFGQGNHEPIFKFDDLFVLKADIVGSKHIKCMLVPNKRGFGNKALSAIAFNSVGTAFEDVLLSPKAKNIA
AIGTLKTNNWQDNYTIQLIIKDILLKE

Sequences:

>Translated_587_residues
MKPQASINGKIWQQKLINEDIVTELCRSFKISDLLARIVSLRVNNLEEVENFLRPKIKNLLPNPFHLLDMEKAVDRTVTA
ILNNQKICIFADYDVDGATSAALLKNIFRDLNILCDIYVPDRIAEGYGPTPFAMQKIKDNGTELLITVDCGAMAHEALKY
AKDVNLDVIVIDHHIATEILPDAVAIVNPNRIDEKSEYKHLAAVGVAFLFATAILSNLKKQNYFTQTMLQPNLMQYLDLV
ALGTVCDMMKLTGLNRAFVASGLKVMQQRQNIGIKTLYDMAGLHEIPKCYHLGFILGPRINAGGRVGKSSLGAHLLSTSC
SNEATKLAKELEKHNNERKVIELLMIEEAMEIALTQQDSSLLFIVKEGWHPGVIGIVAGKLKEKFDKPVAVIALNDGIGK
ASCRSILGIDFGAEIINAKSKDLLIAGGGHAMAAGFTVTAEKLQELQNFFNNAFRISITKLENYKHAEYDIDLTVNSVNF
PLMEELNTLEPFGQGNHEPIFKFDDLFVLKADIVGSKHIKCMLVPNKRGFGNKALSAIAFNSVGTAFEDVLLSPKAKNIA
AIGTLKTNNWQDNYTIQLIIKDILLKE
>Mature_587_residues
MKPQASINGKIWQQKLINEDIVTELCRSFKISDLLARIVSLRVNNLEEVENFLRPKIKNLLPNPFHLLDMEKAVDRTVTA
ILNNQKICIFADYDVDGATSAALLKNIFRDLNILCDIYVPDRIAEGYGPTPFAMQKIKDNGTELLITVDCGAMAHEALKY
AKDVNLDVIVIDHHIATEILPDAVAIVNPNRIDEKSEYKHLAAVGVAFLFATAILSNLKKQNYFTQTMLQPNLMQYLDLV
ALGTVCDMMKLTGLNRAFVASGLKVMQQRQNIGIKTLYDMAGLHEIPKCYHLGFILGPRINAGGRVGKSSLGAHLLSTSC
SNEATKLAKELEKHNNERKVIELLMIEEAMEIALTQQDSSLLFIVKEGWHPGVIGIVAGKLKEKFDKPVAVIALNDGIGK
ASCRSILGIDFGAEIINAKSKDLLIAGGGHAMAAGFTVTAEKLQELQNFFNNAFRISITKLENYKHAEYDIDLTVNSVNF
PLMEELNTLEPFGQGNHEPIFKFDDLFVLKADIVGSKHIKCMLVPNKRGFGNKALSAIAFNSVGTAFEDVLLSPKAKNIA
AIGTLKTNNWQDNYTIQLIIKDILLKE

Specific function: Putative single-stranded-DNA-specific exonuclease. RecA thread formation during DNA double-strand break repair requires RecJ or AadAB [H]

COG id: COG0608

COG function: function code L; Single-stranded DNA-specific exonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 OB DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789259, Length=570, Percent_Identity=33.6842105263158, Blast_Score=256, Evalue=4e-69,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003156
- InterPro:   IPR001667
- InterPro:   IPR004610
- InterPro:   IPR018779 [H]

Pfam domain/function: PF01368 DHH; PF02272 DHHA1; PF10141 ssDNA-exonuc_C [H]

EC number: 3.1.-.-

Molecular weight: Translated: 64960; Mature: 64960

Theoretical pI: Translated: 7.02; Mature: 7.02

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPQASINGKIWQQKLINEDIVTELCRSFKISDLLARIVSLRVNNLEEVENFLRPKIKNL
CCCCCCCCHHHHHHHHCCHHHHHHHHHHCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
LPNPFHLLDMEKAVDRTVTAILNNQKICIFADYDVDGATSAALLKNIFRDLNILCDIYVP
CCCCCEEHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCHHHHHHHHHHHHHHEEEEEECC
DRIAEGYGPTPFAMQKIKDNGTELLITVDCGAMAHEALKYAKDVNLDVIVIDHHIATEIL
HHHHCCCCCCHHHHHHHHCCCCEEEEEEECCHHHHHHHHHHHCCCEEEEEECCHHHHHHC
PDAVAIVNPNRIDEKSEYKHLAAVGVAFLFATAILSNLKKQNYFTQTMLQPNLMQYLDLV
CCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHH
ALGTVCDMMKLTGLNRAFVASGLKVMQQRQNIGIKTLYDMAGLHEIPKCYHLGFILGPRI
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCHHHCCCEEECCCC
NAGGRVGKSSLGAHLLSTSCSNEATKLAKELEKHNNERKVIELLMIEEAMEIALTQQDSS
CCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHEEECCCC
LLFIVKEGWHPGVIGIVAGKLKEKFDKPVAVIALNDGIGKASCRSILGIDFGAEIINAKS
EEEEEECCCCCCCHHHHHHHHHHHHCCCEEEEEEECCCCHHHHHHHHCCCCCHHHHCCCC
KDLLIAGGGHAMAAGFTVTAEKLQELQNFFNNAFRISITKLENYKHAEYDIDLTVNSVNF
CCEEEECCCCEEECCEEEEHHHHHHHHHHHCCEEEEEEEEECCCCCCCEEEEEEEECCCC
PLMEELNTLEPFGQGNHEPIFKFDDLFVLKADIVGSKHIKCMLVPNKRGFGNKALSAIAF
CHHHHHHHCCCCCCCCCCCCEEECCEEEEEEECCCCCCEEEEEECCCCCCCCHHHHHHHH
NSVGTAFEDVLLSPKAKNIAAIGTLKTNNWQDNYTIQLIIKDILLKE
HHHHHHHHHHHCCCCCCCEEEEEEEECCCCCCCCEEEEEEHHHHHCC
>Mature Secondary Structure
MKPQASINGKIWQQKLINEDIVTELCRSFKISDLLARIVSLRVNNLEEVENFLRPKIKNL
CCCCCCCCHHHHHHHHCCHHHHHHHHHHCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
LPNPFHLLDMEKAVDRTVTAILNNQKICIFADYDVDGATSAALLKNIFRDLNILCDIYVP
CCCCCEEHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCHHHHHHHHHHHHHHEEEEEECC
DRIAEGYGPTPFAMQKIKDNGTELLITVDCGAMAHEALKYAKDVNLDVIVIDHHIATEIL
HHHHCCCCCCHHHHHHHHCCCCEEEEEEECCHHHHHHHHHHHCCCEEEEEECCHHHHHHC
PDAVAIVNPNRIDEKSEYKHLAAVGVAFLFATAILSNLKKQNYFTQTMLQPNLMQYLDLV
CCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHH
ALGTVCDMMKLTGLNRAFVASGLKVMQQRQNIGIKTLYDMAGLHEIPKCYHLGFILGPRI
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCHHHCCCEEECCCC
NAGGRVGKSSLGAHLLSTSCSNEATKLAKELEKHNNERKVIELLMIEEAMEIALTQQDSS
CCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHEEECCCC
LLFIVKEGWHPGVIGIVAGKLKEKFDKPVAVIALNDGIGKASCRSILGIDFGAEIINAKS
EEEEEECCCCCCCHHHHHHHHHHHHCCCEEEEEEECCCCHHHHHHHHCCCCCHHHHCCCC
KDLLIAGGGHAMAAGFTVTAEKLQELQNFFNNAFRISITKLENYKHAEYDIDLTVNSVNF
CCEEEECCCCEEECCEEEEHHHHHHHHHHHCCEEEEEEEEECCCCCCCEEEEEEEECCCC
PLMEELNTLEPFGQGNHEPIFKFDDLFVLKADIVGSKHIKCMLVPNKRGFGNKALSAIAF
CHHHHHHHCCCCCCCCCCCCEEECCEEEEEEECCCCCCEEEEEECCCCCCCCHHHHHHHH
NSVGTAFEDVLLSPKAKNIAAIGTLKTNNWQDNYTIQLIIKDILLKE
HHHHHHHHHHHCCCCCCCEEEEEEEECCCCCCCCEEEEEEHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on ester bonds [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]