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The map label for this gene is spoT2 [H]

Identifier: 229586574

GI number: 229586574

Start: 422516

End: 423223

Strand: Direct

Name: spoT2 [H]

Synonym: RAF_ORF0395

Alternate gene names: 229586574

Gene position: 422516-423223 (Clockwise)

Preceding gene: 229586573

Following gene: 229586581

Centisome position: 33.05

GC content: 28.25

Gene sequence:

>708_bases
ATGAAAGATATAGAATATTGGGAAAAAAATTTTGAAAGCTGTAAATATTCAGATCAATTAATCGAAAGGCTTTTATTTTT
AAATACCCAAGTCAAACAACCTATCGATATTAGAGAAGTTCAAAAAGGTATTTATTATGCTCGTAAATATCATGGTGAGC
AAATGCGTCAATCAGGTGACCCTTATTATTCTCATCCAATCGCAGTAACGATTATGGTAGCGGAATTTGTAGCAAAAGAA
GTGCCTAAACTCTTTACTTTTAGAATGCTACAAGCTGCATTACTTCATGACACTATTGAAGATACTGAACTTACTGAAGA
CATAATTAGCAATATTTTTGATAAAGAAGTAGCAAGGCATGTAGCAGGTCTAACTAGAATTAAACCTTGGGGCAAAATAA
GTAGCGAGAAAAGTCTTATTTTATTAATTAAACAAAAAAGATACGATACTGCTCTTATAAAACTTTTTGATAGAATACAT
AATTTACAAACTTTGGAAGCAAAATCGCCTGAAAAAGCTCATAAAATAATTAAAGAGACATTAAAAAGCTTTTTAGTGTT
AAGTGAAATACTTGAAATACCTTCAGTTTCAGAGCTTATATATGCTGAATGTTATAAAAATAATTTAAAATTTAATATAA
ATTCTACTTTTAATAAAATTATAAATTTCGATTCGTTTCCATTTTCTCAAAATAAGTTACTCCCATAA

Upstream 100 bases:

>100_bases
TTAAATATAGAGATTTTTAATAAATAATAATTGAAAATATAAAAGATAATCATCAAAAAAAACAATAAAATAATATATAA
TATATATATGGGACGATTGT

Downstream 100 bases:

>100_bases
AAAAAGTTATACCTACCGGAATGAAAATTAAAAATAATCCCCAATATCCTAGATAATTGGTAATATAAACTAAACCAAAT
GACGTTATAATATAAGTAAA

Product: Guanosine polyphosphate pyrophosphohydrolase/synthetase

Products: NA

Alternate protein names: (p)ppGpp synthase; ATP:GTP 3'-pyrophosphotransferase; ppGpp synthase I [H]

Number of amino acids: Translated: 235; Mature: 235

Protein sequence:

>235_residues
MKDIEYWEKNFESCKYSDQLIERLLFLNTQVKQPIDIREVQKGIYYARKYHGEQMRQSGDPYYSHPIAVTIMVAEFVAKE
VPKLFTFRMLQAALLHDTIEDTELTEDIISNIFDKEVARHVAGLTRIKPWGKISSEKSLILLIKQKRYDTALIKLFDRIH
NLQTLEAKSPEKAHKIIKETLKSFLVLSEILEIPSVSELIYAECYKNNLKFNINSTFNKIINFDSFPFSQNKLLP

Sequences:

>Translated_235_residues
MKDIEYWEKNFESCKYSDQLIERLLFLNTQVKQPIDIREVQKGIYYARKYHGEQMRQSGDPYYSHPIAVTIMVAEFVAKE
VPKLFTFRMLQAALLHDTIEDTELTEDIISNIFDKEVARHVAGLTRIKPWGKISSEKSLILLIKQKRYDTALIKLFDRIH
NLQTLEAKSPEKAHKIIKETLKSFLVLSEILEIPSVSELIYAECYKNNLKFNINSTFNKIINFDSFPFSQNKLLP
>Mature_235_residues
MKDIEYWEKNFESCKYSDQLIERLLFLNTQVKQPIDIREVQKGIYYARKYHGEQMRQSGDPYYSHPIAVTIMVAEFVAKE
VPKLFTFRMLQAALLHDTIEDTELTEDIISNIFDKEVARHVAGLTRIKPWGKISSEKSLILLIKQKRYDTALIKLFDRIH
NLQTLEAKSPEKAHKIIKETLKSFLVLSEILEIPSVSELIYAECYKNNLKFNINSTFNKIINFDSFPFSQNKLLP

Specific function: In eubacteria ppGpp (guanosine 3'-diphosphate 5-' diphosphate) is a mediator of the stringent response that coordinates a variety of cellular activities in response to changes in nutritional abundance. This enzyme catalyzes the formation of pppGpp which i

COG id: COG0317

COG function: function code TK; Guanosine polyphosphate pyrophosphohydrolases/synthetases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HD domain [H]

Homologues:

Organism=Escherichia coli, GI1790082, Length=185, Percent_Identity=32.4324324324324, Blast_Score=89, Evalue=3e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002912
- InterPro:   IPR012675
- InterPro:   IPR003607
- InterPro:   IPR007685
- InterPro:   IPR004811
- InterPro:   IPR004095
- InterPro:   IPR012676 [H]

Pfam domain/function: PF01842 ACT; PF04607 RelA_SpoT; PF02824 TGS [H]

EC number: =2.7.6.5 [H]

Molecular weight: Translated: 27523; Mature: 27523

Theoretical pI: Translated: 8.59; Mature: 8.59

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKDIEYWEKNFESCKYSDQLIERLLFLNTQVKQPIDIREVQKGIYYARKYHGEQMRQSGD
CCCHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCC
PYYSHPIAVTIMVAEFVAKEVPKLFTFRMLQAALLHDTIEDTELTEDIISNIFDKEVARH
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VAGLTRIKPWGKISSEKSLILLIKQKRYDTALIKLFDRIHNLQTLEAKSPEKAHKIIKET
HHHHHHCCCCCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHCCHHHCCCCHHHHHHHHHHH
LKSFLVLSEILEIPSVSELIYAECYKNNLKFNINSTFNKIINFDSFPFSQNKLLP
HHHHHHHHHHHCCCCHHHHHHHHHHHCCCEEEECHHHHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure
MKDIEYWEKNFESCKYSDQLIERLLFLNTQVKQPIDIREVQKGIYYARKYHGEQMRQSGD
CCCHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCC
PYYSHPIAVTIMVAEFVAKEVPKLFTFRMLQAALLHDTIEDTELTEDIISNIFDKEVARH
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VAGLTRIKPWGKISSEKSLILLIKQKRYDTALIKLFDRIHNLQTLEAKSPEKAHKIIKET
HHHHHHCCCCCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHCCHHHCCCCHHHHHHHHHHH
LKSFLVLSEILEIPSVSELIYAECYKNNLKFNINSTFNKIINFDSFPFSQNKLLP
HHHHHHHHHHHCCCCHHHHHHHHHHHCCCEEEECHHHHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]