Click here to switch to the map view.

The map label for this gene is nuoF [H]

Identifier: 229586353

GI number: 229586353

Start: 155834

End: 157099

Strand: Direct

Name: nuoF [H]

Synonym: RAF_ORF0144

Alternate gene names: 229586353

Gene position: 155834-157099 (Clockwise)

Preceding gene: 229586352

Following gene: 229586354

Centisome position: 12.19

GC content: 41.15

Gene sequence:

>1266_bases
ATGCTAAAAGAAGAAGACAAAATTTTTACTAACCTGCATGGGCAGCAAAGCCATGATTTGAAATCTAGCAAGAAGCGAGG
CGATTGGGAGAATACTAAAGCTTTGCTTGATAAAGGTAGAGATTTCATCGTTGAAGAGGTTAAGAAATCAGGGCTTAGAG
GGCGAGGAGGTGCCGGATTTTCTACCGGTATGAAATGGTCATTTATGCCTAAAAATTTAGAGCAGTCTTGCTATTTAGTA
GTAAATGCCGACGAGTCTGAGCCTGGTACTTGTAAGGATCGTGATATATTAAGGTTTGAGCCGCACAAATTAATAGAAGG
TTGCTTGCTTGCAAGCTTTGCTATAGGAGCAAATAATTGTTATATCTATATTAGAGGTGAATTTTATAATGAAGCCTCTA
ATATTCAGCGTGCCTTAGATGAAGCCTATAAAGAAGGGTTAATAGGAAAGAATTCTTGCGGTTCGGGCTTTGATTGTAAT
ATTTATTTACATCGTGGGGCGGGTGCTTATATTTGTGGCGAAGAAACGGCATTGCTTGAAAGCTTAGAGGGCAAAAAAGG
TATGCCTCGTTTAAAGCCGCCTTTTCCTGCCGGCTTTGGGTTATATGGCTGTCCGACTACTATAAATAATGTTGAGTCTA
TCGCAGTAGTGCCAACCATTTTAAGACGAGGAGCTAGCTGGTTTGCTGGTATAGGTAAACCTAACAATACGGGCACTAAG
ATTTTCTGCATATCAGGGCATGTTAACAAGCCTTGTAATGTTGAGGAAGCAATGGGCATTTCGTTAAAAGAATTGATTGA
GAAATATGCGGGTGGTGTTCGTGGTGGATGGGATAATCTTAAAGCCATAATCCCGGGCGGTTCTTCCGTGCCTTTACTTC
CTAAGTCATTATGTGAAGTGGATATGGATTTTGATAGTTTAAGAACAGCAGGTTCTGGACTTGGTACAGGAGGTATTATA
GTTATGGATCAATCTACCGATATTATTTATGCAATAGCAAGGCTTAGTAAATTTTACATGCATGAATCTTGCGGGCAATG
TACTCCTTGCCGTGAGGGTACGGGCTGGATGTGGCGAGTTATGATGCGACTAGTTAAAGGTAATGTCAAAAAATCTGAGA
TAGACGAACTCCTTAACGTTACGAAAGAAATAGAGGGGCATACTATCTGTGCTTTAGGTGATGCGGCAGCTTGGCCGATT
CAAGGGCTGATACGCCATTTTAGAAGCGAGATTGAGGCAAGGATAAAGAGTGATAGTGTGGTTTGA

Upstream 100 bases:

>100_bases
AGCCTGCATGACAATCTCAGGATTTTGGCACGAGGTTGCTTCGTCAAAATTTTCAATTTTTCCTCGCAATGACGTTTTTA
AGCCATAACGAGAATAGTTT

Downstream 100 bases:

>100_bases
CTTTTTATCGTCATTGCGAGGAGATATTGCCTGCGTGGATACTAAGCCGTCATTGCGAACGACTGAAAGGAGAGAGGAGC
GTGGTAATCTCATGAAATAA

Product: NADH dehydrogenase I subunit F

Products: NA

Alternate protein names: NADH dehydrogenase I subunit F; NDH-1 subunit F [H]

Number of amino acids: Translated: 421; Mature: 421

Protein sequence:

>421_residues
MLKEEDKIFTNLHGQQSHDLKSSKKRGDWENTKALLDKGRDFIVEEVKKSGLRGRGGAGFSTGMKWSFMPKNLEQSCYLV
VNADESEPGTCKDRDILRFEPHKLIEGCLLASFAIGANNCYIYIRGEFYNEASNIQRALDEAYKEGLIGKNSCGSGFDCN
IYLHRGAGAYICGEETALLESLEGKKGMPRLKPPFPAGFGLYGCPTTINNVESIAVVPTILRRGASWFAGIGKPNNTGTK
IFCISGHVNKPCNVEEAMGISLKELIEKYAGGVRGGWDNLKAIIPGGSSVPLLPKSLCEVDMDFDSLRTAGSGLGTGGII
VMDQSTDIIYAIARLSKFYMHESCGQCTPCREGTGWMWRVMMRLVKGNVKKSEIDELLNVTKEIEGHTICALGDAAAWPI
QGLIRHFRSEIEARIKSDSVV

Sequences:

>Translated_421_residues
MLKEEDKIFTNLHGQQSHDLKSSKKRGDWENTKALLDKGRDFIVEEVKKSGLRGRGGAGFSTGMKWSFMPKNLEQSCYLV
VNADESEPGTCKDRDILRFEPHKLIEGCLLASFAIGANNCYIYIRGEFYNEASNIQRALDEAYKEGLIGKNSCGSGFDCN
IYLHRGAGAYICGEETALLESLEGKKGMPRLKPPFPAGFGLYGCPTTINNVESIAVVPTILRRGASWFAGIGKPNNTGTK
IFCISGHVNKPCNVEEAMGISLKELIEKYAGGVRGGWDNLKAIIPGGSSVPLLPKSLCEVDMDFDSLRTAGSGLGTGGII
VMDQSTDIIYAIARLSKFYMHESCGQCTPCREGTGWMWRVMMRLVKGNVKKSEIDELLNVTKEIEGHTICALGDAAAWPI
QGLIRHFRSEIEARIKSDSVV
>Mature_421_residues
MLKEEDKIFTNLHGQQSHDLKSSKKRGDWENTKALLDKGRDFIVEEVKKSGLRGRGGAGFSTGMKWSFMPKNLEQSCYLV
VNADESEPGTCKDRDILRFEPHKLIEGCLLASFAIGANNCYIYIRGEFYNEASNIQRALDEAYKEGLIGKNSCGSGFDCN
IYLHRGAGAYICGEETALLESLEGKKGMPRLKPPFPAGFGLYGCPTTINNVESIAVVPTILRRGASWFAGIGKPNNTGTK
IFCISGHVNKPCNVEEAMGISLKELIEKYAGGVRGGWDNLKAIIPGGSSVPLLPKSLCEVDMDFDSLRTAGSGLGTGGII
VMDQSTDIIYAIARLSKFYMHESCGQCTPCREGTGWMWRVMMRLVKGNVKKSEIDELLNVTKEIEGHTICALGDAAAWPI
QGLIRHFRSEIEARIKSDSVV

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cyto

COG id: COG1894

COG function: function code C; NADH:ubiquinone oxidoreductase, NADH-binding (51 kD) subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 51 kDa subunit family [H]

Homologues:

Organism=Homo sapiens, GI260656005, Length=417, Percent_Identity=67.3860911270983, Blast_Score=594, Evalue=1e-170,
Organism=Homo sapiens, GI20149568, Length=417, Percent_Identity=67.3860911270983, Blast_Score=593, Evalue=1e-170,
Organism=Escherichia coli, GI1788620, Length=387, Percent_Identity=44.9612403100775, Blast_Score=339, Evalue=2e-94,
Organism=Caenorhabditis elegans, GI17531727, Length=416, Percent_Identity=64.4230769230769, Blast_Score=573, Evalue=1e-164,
Organism=Drosophila melanogaster, GI19920794, Length=417, Percent_Identity=66.4268585131894, Blast_Score=592, Evalue=1e-169,
Organism=Drosophila melanogaster, GI45550452, Length=417, Percent_Identity=62.3501199040767, Blast_Score=544, Evalue=1e-155,
Organism=Drosophila melanogaster, GI24653819, Length=418, Percent_Identity=48.5645933014354, Blast_Score=405, Evalue=1e-113,
Organism=Drosophila melanogaster, GI24653817, Length=418, Percent_Identity=44.9760765550239, Blast_Score=365, Evalue=1e-101,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001949
- InterPro:   IPR019575
- InterPro:   IPR011537
- InterPro:   IPR011538
- InterPro:   IPR019554 [H]

Pfam domain/function: PF01512 Complex1_51K; PF10589 NADH_4Fe-4S; PF10531 SLBB [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 46140; Mature: 46140

Theoretical pI: Translated: 7.56; Mature: 7.56

Prosite motif: PS00644 COMPLEX1_51K_1 ; PS00645 COMPLEX1_51K_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.6 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
3.6 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
6.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKEEDKIFTNLHGQQSHDLKSSKKRGDWENTKALLDKGRDFIVEEVKKSGLRGRGGAGF
CCCCHHHHHHHCCCCCCCCHHHHHCCCCHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCC
STGMKWSFMPKNLEQSCYLVVNADESEPGTCKDRDILRFEPHKLIEGCLLASFAIGANNC
CCCCEEEECCCCCCCCEEEEEECCCCCCCCCCCCCCEEECHHHHHHHHHHHHHHCCCCCE
YIYIRGEFYNEASNIQRALDEAYKEGLIGKNSCGSGFDCNIYLHRGAGAYICGEETALLE
EEEEEECHHCHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEECCCCEEEECCHHHHHH
SLEGKKGMPRLKPPFPAGFGLYGCPTTINNVESIAVVPTILRRGASWFAGIGKPNNTGTK
HHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCHHEECCCCCCCCCCE
IFCISGHVNKPCNVEEAMGISLKELIEKYAGGVRGGWDNLKAIIPGGSSVPLLPKSLCEV
EEEEECCCCCCCCHHHHHCCCHHHHHHHHCCCCCCCCCCCEEEECCCCCCCCCCHHHHHH
DMDFDSLRTAGSGLGTGGIIVMDQSTDIIYAIARLSKFYMHESCGQCTPCREGTGWMWRV
CCCHHHHHHCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHH
MMRLVKGNVKKSEIDELLNVTKEIEGHTICALGDAAAWPIQGLIRHFRSEIEARIKSDSV
HHHHHHCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCC
V
C
>Mature Secondary Structure
MLKEEDKIFTNLHGQQSHDLKSSKKRGDWENTKALLDKGRDFIVEEVKKSGLRGRGGAGF
CCCCHHHHHHHCCCCCCCCHHHHHCCCCHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCC
STGMKWSFMPKNLEQSCYLVVNADESEPGTCKDRDILRFEPHKLIEGCLLASFAIGANNC
CCCCEEEECCCCCCCCEEEEEECCCCCCCCCCCCCCEEECHHHHHHHHHHHHHHCCCCCE
YIYIRGEFYNEASNIQRALDEAYKEGLIGKNSCGSGFDCNIYLHRGAGAYICGEETALLE
EEEEEECHHCHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEECCCCEEEECCHHHHHH
SLEGKKGMPRLKPPFPAGFGLYGCPTTINNVESIAVVPTILRRGASWFAGIGKPNNTGTK
HHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCHHEECCCCCCCCCCE
IFCISGHVNKPCNVEEAMGISLKELIEKYAGGVRGGWDNLKAIIPGGSSVPLLPKSLCEV
EEEEECCCCCCCCHHHHHCCCHHHHHHHHCCCCCCCCCCCEEEECCCCCCCCCCHHHHHH
DMDFDSLRTAGSGLGTGGIIVMDQSTDIIYAIARLSKFYMHESCGQCTPCREGTGWMWRV
CCCHHHHHHCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHH
MMRLVKGNVKKSEIDELLNVTKEIEGHTICALGDAAAWPIQGLIRHFRSEIEARIKSDSV
HHHHHHCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCC
V
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA