Click here to switch to the map view.

The map label for this gene is lepB [H]

Identifier: 229586354

GI number: 229586354

Start: 157283

End: 158083

Strand: Direct

Name: lepB [H]

Synonym: RAF_ORF0145

Alternate gene names: 229586354

Gene position: 157283-158083 (Clockwise)

Preceding gene: 229586353

Following gene: 229586355

Centisome position: 12.3

GC content: 31.59

Gene sequence:

>801_bases
ATGCAAACAGATAATACAAAATCAAACACTAATAAAACAGCTAAGCAAGAGTGGGGGTCTTTTGCTTTTGTAATATGTAT
CGCTTTACTTATTAGAATACTTATTATGGAGCCGTTTACCGTTCCGACCGGGTCTATGAAAGCAACCATACTTGAGAATG
ATTATATATTTTCTACGAAATATAGTTATGGTTATAGTAATTATTCTTTATCTTTTTTTGATTTTATTCCTCTTTTTAAA
GGGCGCATATTTGCTCGTGAACCGGATCGTGGCGATATTGTAGTTTTTCGTCCGCCTAACGATATGAGTGTTAGATATAT
AAAGCGTTTAATAGGGTTACCCGGTGATAAAATTCAATTGATTGATGACGTAATATATATTAACGACAAAAAAATAGAAC
GCACGGAAGTTGGAACTTATATAAGCGAAGAGGGAATAAAATATTTAAAGTTTAAAGAAACACTGCCAAACGGTAGAACA
TATTTTTCTTATAAGCTTGCTCCTATTTATGGTGTTATATATAATGATAGATACGGTAATACGGATGTTTTTTATGTACC
TGAAGGGCAATATTTCTTTTTAGGGGATAATAGAGATCAGTCAAATGATAGTAGAGTAAATCTTGGATTTGTACCGTTTG
AAAATTTTATTGCTAAAGCACAATTTATTTGGTTCTCAACAAAAATAACTTGGTGGGATAATGATATAGGAGTTATTAAT
CTAGTACTAAAGTTAAAGCCATGGATTGAATCTGTTAGGTTAAATCGAATTTTCAGAAATCTTTATAACACGGATGCGTA
A

Upstream 100 bases:

>100_bases
GTAATCTCATGAAATAATAACAAACTCCTGAGATTGCTTCGTCAATTACTTTATAATTTCCTCGCAATGACGATTCCAAT
TTATAAATAATTAAGAGATT

Downstream 100 bases:

>100_bases
TGGAATCATTTGAGAAGTTAGAAAAGCTACTTAGCTATAGTTTCAAAAATAAAGAGCTTTTAATAGAGGCGTTGAGTCAC
CCGTCTTTAAGGCAACATCA

Product: Signal peptidase I

Products: NA

Alternate protein names: SPase I; Leader peptidase I [H]

Number of amino acids: Translated: 266; Mature: 266

Protein sequence:

>266_residues
MQTDNTKSNTNKTAKQEWGSFAFVICIALLIRILIMEPFTVPTGSMKATILENDYIFSTKYSYGYSNYSLSFFDFIPLFK
GRIFAREPDRGDIVVFRPPNDMSVRYIKRLIGLPGDKIQLIDDVIYINDKKIERTEVGTYISEEGIKYLKFKETLPNGRT
YFSYKLAPIYGVIYNDRYGNTDVFYVPEGQYFFLGDNRDQSNDSRVNLGFVPFENFIAKAQFIWFSTKITWWDNDIGVIN
LVLKLKPWIESVRLNRIFRNLYNTDA

Sequences:

>Translated_266_residues
MQTDNTKSNTNKTAKQEWGSFAFVICIALLIRILIMEPFTVPTGSMKATILENDYIFSTKYSYGYSNYSLSFFDFIPLFK
GRIFAREPDRGDIVVFRPPNDMSVRYIKRLIGLPGDKIQLIDDVIYINDKKIERTEVGTYISEEGIKYLKFKETLPNGRT
YFSYKLAPIYGVIYNDRYGNTDVFYVPEGQYFFLGDNRDQSNDSRVNLGFVPFENFIAKAQFIWFSTKITWWDNDIGVIN
LVLKLKPWIESVRLNRIFRNLYNTDA
>Mature_266_residues
MQTDNTKSNTNKTAKQEWGSFAFVICIALLIRILIMEPFTVPTGSMKATILENDYIFSTKYSYGYSNYSLSFFDFIPLFK
GRIFAREPDRGDIVVFRPPNDMSVRYIKRLIGLPGDKIQLIDDVIYINDKKIERTEVGTYISEEGIKYLKFKETLPNGRT
YFSYKLAPIYGVIYNDRYGNTDVFYVPEGQYFFLGDNRDQSNDSRVNLGFVPFENFIAKAQFIWFSTKITWWDNDIGVIN
LVLKLKPWIESVRLNRIFRNLYNTDA

Specific function: Unknown

COG id: COG0681

COG function: function code U; Signal peptidase I

Gene ontology:

Cell location: Cell inner membrane; Single-pass type II membrane protein (Potential) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S26 family [H]

Homologues:

Organism=Escherichia coli, GI1788921, Length=285, Percent_Identity=32.280701754386, Blast_Score=113, Evalue=1e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000223
- InterPro:   IPR019758
- InterPro:   IPR019757
- InterPro:   IPR019759
- InterPro:   IPR015927
- InterPro:   IPR011056 [H]

Pfam domain/function: PF00717 Peptidase_S24 [H]

EC number: =3.4.21.89 [H]

Molecular weight: Translated: 31097; Mature: 31097

Theoretical pI: Translated: 9.00; Mature: 9.00

Prosite motif: PS00760 SPASE_I_2 ; PS00761 SPASE_I_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQTDNTKSNTNKTAKQEWGSFAFVICIALLIRILIMEPFTVPTGSMKATILENDYIFSTK
CCCCCCCCCCCCHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCEEEEEE
YSYGYSNYSLSFFDFIPLFKGRIFAREPDRGDIVVFRPPNDMSVRYIKRLIGLPGDKIQL
ECCCCCCCCCHHHHHHHHHCCEEEEECCCCCCEEEEECCCCCHHHHHHHHHCCCCCCEEE
IDDVIYINDKKIERTEVGTYISEEGIKYLKFKETLPNGRTYFSYKLAPIYGVIYNDRYGN
EEEEEEECCCEEEHHHHCCEECCCCCEEEEEECCCCCCCEEEEEEEEEEEEEEEECCCCC
TDVFYVPEGQYFFLGDNRDQSNDSRVNLGFVPFENFIAKAQFIWFSTKITWWDNDIGVIN
CEEEEEECCCEEEEECCCCCCCCCEEEEEEECHHHHHHEEEEEEEEEEEEEECCCCCEEE
LVLKLKPWIESVRLNRIFRNLYNTDA
EEEEEHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MQTDNTKSNTNKTAKQEWGSFAFVICIALLIRILIMEPFTVPTGSMKATILENDYIFSTK
CCCCCCCCCCCCHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCEEEEEE
YSYGYSNYSLSFFDFIPLFKGRIFAREPDRGDIVVFRPPNDMSVRYIKRLIGLPGDKIQL
ECCCCCCCCCHHHHHHHHHCCEEEEECCCCCCEEEEECCCCCHHHHHHHHHCCCCCCEEE
IDDVIYINDKKIERTEVGTYISEEGIKYLKFKETLPNGRTYFSYKLAPIYGVIYNDRYGN
EEEEEEECCCEEEHHHHCCEECCCCCEEEEEECCCCCCCEEEEEEEEEEEEEEEECCCCC
TDVFYVPEGQYFFLGDNRDQSNDSRVNLGFVPFENFIAKAQFIWFSTKITWWDNDIGVIN
CEEEEEECCCEEEEECCCCCCCCCEEEEEEECHHHHHHEEEEEEEEEEEEEECCCCCEEE
LVLKLKPWIESVRLNRIFRNLYNTDA
EEEEEHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA