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The map label for this gene is queF

Identifier: 229586315

GI number: 229586315

Start: 96993

End: 97814

Strand: Direct

Name: queF

Synonym: RAF_ORF0098

Alternate gene names: 229586315

Gene position: 96993-97814 (Clockwise)

Preceding gene: 229586314

Following gene: 229586319

Centisome position: 7.59

GC content: 29.2

Gene sequence:

>822_bases
ATGCCTTTATCTACTTCTTTACTTGGTAAAAAAAGTACTTATAAAGATAGTTATGATGTAACTTTATTATTTAAAATTCC
ACGTATAAATAATCGAAACGAACTTGGAATAAATAGTAATAATCTTCCGTTTTATGGTGTAGATGTTTGGAATACATATG
AACTATCTTGTCTTAATAAGAACGGTAAGCCATGGGTTGGAGTAGGCACTTTTTATATACCGACGGATTCTGAAAATATA
GTAGAATCAAAATCATTTAAATTATATCTAAATTCTTTTAATAACTTTGTTGTTGAATCAGTAAAAGAACTAGAAAGAAT
TATCTTGCAGGATCTAAGTAATGTTACACACGCTAAAGTAACAGGACGTATATTTTCTATAAATACAAAAGTAGAATTTG
GTGTTCCAAGCGGTAAAAATATCGATGATTTAGATATAGTATGCAATAATTACGGTGCACCCGATAATAGTTTAATTGAG
TATGAAGATGTTTTGGTAGAAGAAGAGATTAATTCTAATTTACTCAAATCAAATTGCCTAGTAACCGGTCAACCGGATTG
GGGTACAATCGTTATAAAATATAAAGGAAAAAAGTTAAAATATGATTCTTTTCTGAAATATTTAATATCTTTTAGAAATT
GTAATGAGTTTGCAGAGCAGTGTGCTGAGCGTATTTTTACAGATATTAAAAATGCTATAAGTCCTGATTTTCTTTCTATT
TATATAGTATATGCTAGGCGTGGAGGCATTGATATTTGTCCCTATCGTTCTACCGATAAAAGTTATACTTTACCTAGCGA
TAAACGATTTATTAGGCAATAA

Upstream 100 bases:

>100_bases
TGGTAGAGATTATATCGACACAGTATGGGGACGTGGTTATATGCTAAAAGAATATGATGAGTTACAACAAAAAGAAATTT
TAGCACAAGGAGCATAATTA

Downstream 100 bases:

>100_bases
TCACTTTTCTAATCCATAAAAAAAATTCGTTTGTGCGAAAAGTATTGTCAGTGTGGATTGAAAAACGCATCCGTTGTCAT
TCCGTGGCTTGACACAGTGG

Product: 7-cyano-7-deazaguanine reductase

Products: NA

Alternate protein names: 7-cyano-7-carbaguanine reductase; NADPH-dependent nitrile oxidoreductase; PreQ(0) reductase

Number of amino acids: Translated: 273; Mature: 272

Protein sequence:

>273_residues
MPLSTSLLGKKSTYKDSYDVTLLFKIPRINNRNELGINSNNLPFYGVDVWNTYELSCLNKNGKPWVGVGTFYIPTDSENI
VESKSFKLYLNSFNNFVVESVKELERIILQDLSNVTHAKVTGRIFSINTKVEFGVPSGKNIDDLDIVCNNYGAPDNSLIE
YEDVLVEEEINSNLLKSNCLVTGQPDWGTIVIKYKGKKLKYDSFLKYLISFRNCNEFAEQCAERIFTDIKNAISPDFLSI
YIVYARRGGIDICPYRSTDKSYTLPSDKRFIRQ

Sequences:

>Translated_273_residues
MPLSTSLLGKKSTYKDSYDVTLLFKIPRINNRNELGINSNNLPFYGVDVWNTYELSCLNKNGKPWVGVGTFYIPTDSENI
VESKSFKLYLNSFNNFVVESVKELERIILQDLSNVTHAKVTGRIFSINTKVEFGVPSGKNIDDLDIVCNNYGAPDNSLIE
YEDVLVEEEINSNLLKSNCLVTGQPDWGTIVIKYKGKKLKYDSFLKYLISFRNCNEFAEQCAERIFTDIKNAISPDFLSI
YIVYARRGGIDICPYRSTDKSYTLPSDKRFIRQ
>Mature_272_residues
PLSTSLLGKKSTYKDSYDVTLLFKIPRINNRNELGINSNNLPFYGVDVWNTYELSCLNKNGKPWVGVGTFYIPTDSENIV
ESKSFKLYLNSFNNFVVESVKELERIILQDLSNVTHAKVTGRIFSINTKVEFGVPSGKNIDDLDIVCNNYGAPDNSLIEY
EDVLVEEEINSNLLKSNCLVTGQPDWGTIVIKYKGKKLKYDSFLKYLISFRNCNEFAEQCAERIFTDIKNAISPDFLSIY
IVYARRGGIDICPYRSTDKSYTLPSDKRFIRQ

Specific function: Catalyzes the NADPH-dependent reduction of 7-cyano-7- deazaguanine (preQ0) to 7-aminomethyl-7-deazaguanine (preQ1)

COG id: COG0780

COG function: function code R; Enzyme related to GTP cyclohydrolase I

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP cyclohydrolase I family. QueF type 2 subfamily

Homologues:

Organism=Escherichia coli, GI1789158, Length=277, Percent_Identity=43.6823104693141, Blast_Score=232, Evalue=2e-62,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): QUEF_RICAE (C3PMB3)

Other databases:

- EMBL:   CP001612
- RefSeq:   YP_002844816.1
- ProteinModelPortal:   C3PMB3
- GeneID:   7815349
- GenomeReviews:   CP001612_GR
- KEGG:   raf:RAF_ORF0098
- OMA:   PFRSNFE
- ProtClustDB:   PRK11792
- GO:   GO:0005737
- HAMAP:   MF_00817
- InterPro:   IPR016428
- InterPro:   IPR020602
- PIRSF:   PIRSF004750
- TIGRFAMs:   TIGR03138

Pfam domain/function: PF01227 GTP_cyclohydroI

EC number: =1.7.1.13

Molecular weight: Translated: 31182; Mature: 31051

Theoretical pI: Translated: 7.05; Mature: 7.05

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
0.4 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPLSTSLLGKKSTYKDSYDVTLLFKIPRINNRNELGINSNNLPFYGVDVWNTYELSCLNK
CCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCEEEEECCCEEEEEEECC
NGKPWVGVGTFYIPTDSENIVESKSFKLYLNSFNNFVVESVKELERIILQDLSNVTHAKV
CCCCEEEEEEEEEECCCCHHHCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHCCCEEEE
TGRIFSINTKVEFGVPSGKNIDDLDIVCNNYGAPDNSLIEYEDVLVEEEINSNLLKSNCL
EEEEEEEEEEEEECCCCCCCCCHHHEEECCCCCCCCCEEEHHHHHHHHHHCCHHHHCCCE
VTGQPDWGTIVIKYKGKKLKYDSFLKYLISFRNCNEFAEQCAERIFTDIKNAISPDFLSI
EECCCCCCEEEEEECCCEECHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCCCCEEE
YIVYARRGGIDICPYRSTDKSYTLPSDKRFIRQ
EEEEEECCCCEECCCCCCCCCCCCCCHHHHHCC
>Mature Secondary Structure 
PLSTSLLGKKSTYKDSYDVTLLFKIPRINNRNELGINSNNLPFYGVDVWNTYELSCLNK
CCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCEEEEECCCEEEEEEECC
NGKPWVGVGTFYIPTDSENIVESKSFKLYLNSFNNFVVESVKELERIILQDLSNVTHAKV
CCCCEEEEEEEEEECCCCHHHCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHCCCEEEE
TGRIFSINTKVEFGVPSGKNIDDLDIVCNNYGAPDNSLIEYEDVLVEEEINSNLLKSNCL
EEEEEEEEEEEEECCCCCCCCCHHHEEECCCCCCCCCEEEHHHHHHHHHHCCHHHHCCCE
VTGQPDWGTIVIKYKGKKLKYDSFLKYLISFRNCNEFAEQCAERIFTDIKNAISPDFLSI
EECCCCCCEEEEEECCCEECHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCCCCEEE
YIVYARRGGIDICPYRSTDKSYTLPSDKRFIRQ
EEEEEECCCCEECCCCCCCCCCCCCCHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA