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The map label for this gene is gidB [H]

Identifier: 229586297

GI number: 229586297

Start: 78911

End: 79489

Strand: Direct

Name: gidB [H]

Synonym: RAF_ORF0080

Alternate gene names: 229586297

Gene position: 78911-79489 (Clockwise)

Preceding gene: 229586296

Following gene: 229586298

Centisome position: 6.17

GC content: 25.73

Gene sequence:

>579_bases
GTGATGGAAGTTCCACGTGAAATAATTGAAAAGTTAGAAATTTTTCAAAAGTTAGTTAAAAAATGGAATAAATCAATCAA
TCTTGTATCGGATAATACTATACATAATTTTTGGCAACGTCATATATTAGACTCATTACAATTAATACAATATATTGATA
ATAAAGAAATACATTTGGTTGATATAGGAAGCGGTTCCGGTTTGCCTGGTATAGTATTATCTATAGCGGGGGTAGCGCAA
GTAAGTTTAATAGAAGCTGATTTGCGTAAATGTATATTTTTAGAAAAAGCATCAAAGATATCAAATAATAACATACAAAT
AATCAATCAAAGAATAGAAAAAGTAGAAATAGATTGTAGTATATTAACTTGTAGGGCTTTCTCTAACTTAAATACAATAT
TTAATTGCATAAAAAATATTTCAGTACGAGAAAAATTTTTGTTATTAAAAGGAAAAAATTATTTAACTGAAATAGTAGAA
GCTAAAGAGAGATGGTTATTTGGTTATTTAATACATCAAAGTATTACTTGTGAAGAAGGGAAAATTCTAGAAGTTAGTAA
TTTAACAAAAATGATATGA

Upstream 100 bases:

>100_bases
TCTTCCCATAAACCTACTACTATTGGTGTAGCACGTCGAATTCCAGGGATTACACCTGCAGCAATTACGGCTATTATAAT
TTATTTAAAAACTAAATATA

Downstream 100 bases:

>100_bases
AAGTAATTGCTATTGTTAATCAAAAAGGAGGGGTAGCTAAAACTACAACTACTGTGAATTTAGCTACAGCTTTTGCTGCT
GTAAATAAAAAAATTTTAGT

Product: 16S rRNA methyltransferase GidB

Products: NA

Alternate protein names: 16S rRNA 7-methylguanosine methyltransferase; 16S rRNA m7G methyltransferase [H]

Number of amino acids: Translated: 192; Mature: 192

Protein sequence:

>192_residues
MMEVPREIIEKLEIFQKLVKKWNKSINLVSDNTIHNFWQRHILDSLQLIQYIDNKEIHLVDIGSGSGLPGIVLSIAGVAQ
VSLIEADLRKCIFLEKASKISNNNIQIINQRIEKVEIDCSILTCRAFSNLNTIFNCIKNISVREKFLLLKGKNYLTEIVE
AKERWLFGYLIHQSITCEEGKILEVSNLTKMI

Sequences:

>Translated_192_residues
MMEVPREIIEKLEIFQKLVKKWNKSINLVSDNTIHNFWQRHILDSLQLIQYIDNKEIHLVDIGSGSGLPGIVLSIAGVAQ
VSLIEADLRKCIFLEKASKISNNNIQIINQRIEKVEIDCSILTCRAFSNLNTIFNCIKNISVREKFLLLKGKNYLTEIVE
AKERWLFGYLIHQSITCEEGKILEVSNLTKMI
>Mature_192_residues
MMEVPREIIEKLEIFQKLVKKWNKSINLVSDNTIHNFWQRHILDSLQLIQYIDNKEIHLVDIGSGSGLPGIVLSIAGVAQ
VSLIEADLRKCIFLEKASKISNNNIQIINQRIEKVEIDCSILTCRAFSNLNTIFNCIKNISVREKFLLLKGKNYLTEIVE
AKERWLFGYLIHQSITCEEGKILEVSNLTKMI

Specific function: Specifically methylates the N7 position of guanosine in position 527 of 16S rRNA [H]

COG id: COG0357

COG function: function code M; Predicted S-adenosylmethionine-dependent methyltransferase involved in bacterial cell division

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RNA methyltransferase rsmG family [H]

Homologues:

Organism=Escherichia coli, GI1790179, Length=172, Percent_Identity=27.3255813953488, Blast_Score=70, Evalue=9e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003682 [H]

Pfam domain/function: PF02527 GidB [H]

EC number: =2.1.1.170 [H]

Molecular weight: Translated: 22084; Mature: 22084

Theoretical pI: Translated: 8.21; Mature: 8.21

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMEVPREIIEKLEIFQKLVKKWNKSINLVSDNTIHNFWQRHILDSLQLIQYIDNKEIHLV
CCCCHHHHHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHHHHHHHCCCCEEEE
DIGSGSGLPGIVLSIAGVAQVSLIEADLRKCIFLEKASKISNNNIQIINQRIEKVEIDCS
EECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCE
ILTCRAFSNLNTIFNCIKNISVREKFLLLKGKNYLTEIVEAKERWLFGYLIHQSITCEEG
EEEHHHHHHHHHHHHHHHCCCHHHHHEEEECCHHHHHHHHHHHHHHHHHHHHCCCCCCCC
KILEVSNLTKMI
CEEEHHHHHHCC
>Mature Secondary Structure
MMEVPREIIEKLEIFQKLVKKWNKSINLVSDNTIHNFWQRHILDSLQLIQYIDNKEIHLV
CCCCHHHHHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHHHHHHHCCCCEEEE
DIGSGSGLPGIVLSIAGVAQVSLIEADLRKCIFLEKASKISNNNIQIINQRIEKVEIDCS
EECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCE
ILTCRAFSNLNTIFNCIKNISVREKFLLLKGKNYLTEIVEAKERWLFGYLIHQSITCEEG
EEEHHHHHHHHHHHHHHHCCCHHHHHEEEECCHHHHHHHHHHHHHHHHHHHHCCCCCCCC
KILEVSNLTKMI
CEEEHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA