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The map label for this gene is gidA

Identifier: 229586296

GI number: 229586296

Start: 77062

End: 78930

Strand: Direct

Name: gidA

Synonym: RAF_ORF0079

Alternate gene names: 229586296

Gene position: 77062-78930 (Clockwise)

Preceding gene: 229586295

Following gene: 229586297

Centisome position: 6.03

GC content: 32.74

Gene sequence:

>1869_bases
ATGCTAAAATATGACGTAATAGTTATAGGCGGTGGACATGCAGGCGTAGAAGCAGCCGCTGCTTCTGCGCGTCTTGGAGT
TCCTACTCTCTTAATTACGTTAAAACCGGAAAATTTAGGCGAAATGTCTTGCAATCCAGCAATCGGTGGCATTGCAAAAG
GCACATTAGTTAAAGAAATCGATGCACTTGATGGGTTAATGGGTTATGTTATAGACCAAGCCGGTATACATTATAAAATG
CTGAATGAGACTAGAGGACCAGCAGTTTGGGGACCTAGAGCTCAAGCTGATCGAAAGCTTTATAAAAAAGCAATGTATCA
AATATTAACAAATTATCCTAATTTAGATATATTATATGGAAAAGTGGAAGATATAGAAATTAAATCTTCTAAAATTGAAG
CGGTTATTTTAAATAACGGTAGCAAAATTCTTTGTCAAAAAATTATATTAACTACAGGTACTTTCCTATCAGGTCTTATT
CATATTGGACAAAAAAAAATTCCTGCAGGTAGAGTAGATGAAGAGCCTTCGTATGGATTATCAAATACATTAAAACAAAT
AGGTTTTAAACTGGCTCGTTTAAAAACAGGTACACCGCCTCGCATTGATGGACGTACTATTGATTATAGTAAAACAATTT
TACAACCCGGTGATAAAATTCCTCGTCCTTTTTCTGAATTAACAAATATTGTTAATGTTTCTCAGATAAATTGTTTTATC
ACAAAAACAACCTCAGAAACTCATGATATTATACGTGAGAATCTTGATAAATCCGCAATGTATTCAGGGCAAATAGAAGG
AATAGGACCACGATATTGTCCTTCTATTGAAGATAAAATAGTCAGATTTAGTACAAAATCAGAACATCGTATATTTTTAG
AACCTGAAGGGTTAGATGATTATACGATTTATCCAAACGGTATTTCTACTTCTTTGCCTGAAGATGTGCAACATAAATTA
ATCAAAACAATACCAGGATTAGAAAATGTAAAGGTTTTACGTCCAGGTTATGCTATAGAATATGATTATGTTGATCCACG
TGAAATAAGTGTTACGTTAGAGACAAAAAAAATAGCTGGATTGTATTTGGCCGGGCAAATTAACGGTACTACAGGATATG
AGGAAGCCGCAGGGCAAGGTATAATAGCCGGTATAAATGCTGCTTTAGCAGTAAAAGATCAAGCACCGTTTATGTTAACA
AGAGCAAACAGTTATATTGGAGTAATGATTGACGATTTAACAACATTTGGTACTATAGAACCTTATCGTATGTTTACTTC
TCGCTCAGAATATAGATTATCTTTAAGAGCAGATAACTCTGATTTACGTTTAACGGAATTAGGGATGAACATTGGAGTCG
TATCAGAAAAACGTAAAAAAATATTCACAAAAAAATGTGAAGACATAGAAAAAATAAAATCATTATTAAATACCTTGTCT
TTAACTACTAGTAAACTTGCTAAAATGGGTATACAGGTTGCACAGGATGGTACATATAAAACAGTATTAGACTTATTTAA
AATACCTAATTTTAATGTTGAACAAGCTATTAAAATTTTTCCTATGCTTAAAGAAACACAAAATAAGAACATTTTGCAAT
TACTCTATATTGAAGCAAAATATGCTTCATATTTAACTAGACAACACGCAGATATTAATTTATTTCAAAGTGAAGAGGCG
CAGTTCATACCTAAAAATATTGACTATTTTAAAATACCAAGTATATCATTAGAGATACAGGAAAAACTTTCTTCCCATAA
ACCTACTACTATTGGTGTAGCACGTCGAATTCCAGGGATTACACCTGCAGCAATTACGGCTATTATAATTTATTTAAAAA
CTAAATATAGTGATGGAAGTTCCACGTGA

Upstream 100 bases:

>100_bases
ATCAATTGAGGCTAATAGTATTCATGGTTCAGATAGTGAAAATAGTGCGAAAAGAGAAATCGAATTTTTCTTTAATAAAT
CTGAAATTATAGAATAATTA

Downstream 100 bases:

>100_bases
AATAATTGAAAAGTTAGAAATTTTTCAAAAGTTAGTTAAAAAATGGAATAAATCAATCAATCTTGTATCGGATAATACTA
TACATAATTTTTGGCAACGT

Product: tRNA uridine 5-carboxymethylaminomethyl modification enzyme GidA

Products: NA

Alternate protein names: Glucose-inhibited division protein A

Number of amino acids: Translated: 622; Mature: 622

Protein sequence:

>622_residues
MLKYDVIVIGGGHAGVEAAAASARLGVPTLLITLKPENLGEMSCNPAIGGIAKGTLVKEIDALDGLMGYVIDQAGIHYKM
LNETRGPAVWGPRAQADRKLYKKAMYQILTNYPNLDILYGKVEDIEIKSSKIEAVILNNGSKILCQKIILTTGTFLSGLI
HIGQKKIPAGRVDEEPSYGLSNTLKQIGFKLARLKTGTPPRIDGRTIDYSKTILQPGDKIPRPFSELTNIVNVSQINCFI
TKTTSETHDIIRENLDKSAMYSGQIEGIGPRYCPSIEDKIVRFSTKSEHRIFLEPEGLDDYTIYPNGISTSLPEDVQHKL
IKTIPGLENVKVLRPGYAIEYDYVDPREISVTLETKKIAGLYLAGQINGTTGYEEAAGQGIIAGINAALAVKDQAPFMLT
RANSYIGVMIDDLTTFGTIEPYRMFTSRSEYRLSLRADNSDLRLTELGMNIGVVSEKRKKIFTKKCEDIEKIKSLLNTLS
LTTSKLAKMGIQVAQDGTYKTVLDLFKIPNFNVEQAIKIFPMLKETQNKNILQLLYIEAKYASYLTRQHADINLFQSEEA
QFIPKNIDYFKIPSISLEIQEKLSSHKPTTIGVARRIPGITPAAITAIIIYLKTKYSDGSST

Sequences:

>Translated_622_residues
MLKYDVIVIGGGHAGVEAAAASARLGVPTLLITLKPENLGEMSCNPAIGGIAKGTLVKEIDALDGLMGYVIDQAGIHYKM
LNETRGPAVWGPRAQADRKLYKKAMYQILTNYPNLDILYGKVEDIEIKSSKIEAVILNNGSKILCQKIILTTGTFLSGLI
HIGQKKIPAGRVDEEPSYGLSNTLKQIGFKLARLKTGTPPRIDGRTIDYSKTILQPGDKIPRPFSELTNIVNVSQINCFI
TKTTSETHDIIRENLDKSAMYSGQIEGIGPRYCPSIEDKIVRFSTKSEHRIFLEPEGLDDYTIYPNGISTSLPEDVQHKL
IKTIPGLENVKVLRPGYAIEYDYVDPREISVTLETKKIAGLYLAGQINGTTGYEEAAGQGIIAGINAALAVKDQAPFMLT
RANSYIGVMIDDLTTFGTIEPYRMFTSRSEYRLSLRADNSDLRLTELGMNIGVVSEKRKKIFTKKCEDIEKIKSLLNTLS
LTTSKLAKMGIQVAQDGTYKTVLDLFKIPNFNVEQAIKIFPMLKETQNKNILQLLYIEAKYASYLTRQHADINLFQSEEA
QFIPKNIDYFKIPSISLEIQEKLSSHKPTTIGVARRIPGITPAAITAIIIYLKTKYSDGSST
>Mature_622_residues
MLKYDVIVIGGGHAGVEAAAASARLGVPTLLITLKPENLGEMSCNPAIGGIAKGTLVKEIDALDGLMGYVIDQAGIHYKM
LNETRGPAVWGPRAQADRKLYKKAMYQILTNYPNLDILYGKVEDIEIKSSKIEAVILNNGSKILCQKIILTTGTFLSGLI
HIGQKKIPAGRVDEEPSYGLSNTLKQIGFKLARLKTGTPPRIDGRTIDYSKTILQPGDKIPRPFSELTNIVNVSQINCFI
TKTTSETHDIIRENLDKSAMYSGQIEGIGPRYCPSIEDKIVRFSTKSEHRIFLEPEGLDDYTIYPNGISTSLPEDVQHKL
IKTIPGLENVKVLRPGYAIEYDYVDPREISVTLETKKIAGLYLAGQINGTTGYEEAAGQGIIAGINAALAVKDQAPFMLT
RANSYIGVMIDDLTTFGTIEPYRMFTSRSEYRLSLRADNSDLRLTELGMNIGVVSEKRKKIFTKKCEDIEKIKSLLNTLS
LTTSKLAKMGIQVAQDGTYKTVLDLFKIPNFNVEQAIKIFPMLKETQNKNILQLLYIEAKYASYLTRQHADINLFQSEEA
QFIPKNIDYFKIPSISLEIQEKLSSHKPTTIGVARRIPGITPAAITAIIIYLKTKYSDGSST

Specific function: NAD-binding protein involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA-cmnm(5)s(2)U34

COG id: COG0445

COG function: function code D; NAD/FAD-utilizing enzyme apparently involved in cell division

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MnmG family

Homologues:

Organism=Homo sapiens, GI74024895, Length=631, Percent_Identity=46.4342313787639, Blast_Score=537, Evalue=1e-152,
Organism=Homo sapiens, GI19882217, Length=656, Percent_Identity=44.6646341463415, Blast_Score=523, Evalue=1e-148,
Organism=Homo sapiens, GI183227703, Length=671, Percent_Identity=43.6661698956781, Blast_Score=519, Evalue=1e-147,
Organism=Escherichia coli, GI2367273, Length=617, Percent_Identity=49.1085899513776, Blast_Score=607, Evalue=1e-175,
Organism=Caenorhabditis elegans, GI17534255, Length=616, Percent_Identity=43.1818181818182, Blast_Score=497, Evalue=1e-141,
Organism=Saccharomyces cerevisiae, GI6321202, Length=624, Percent_Identity=47.4358974358974, Blast_Score=583, Evalue=1e-167,
Organism=Drosophila melanogaster, GI24658174, Length=629, Percent_Identity=47.0588235294118, Blast_Score=536, Evalue=1e-152,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MNMG_RICAE (C3PM94)

Other databases:

- EMBL:   CP001612
- RefSeq:   YP_002844797.1
- ProteinModelPortal:   C3PM94
- GeneID:   7815922
- GenomeReviews:   CP001612_GR
- KEGG:   raf:RAF_ORF0079
- OMA:   GIQFRVL
- ProtClustDB:   PRK05192
- GO:   GO:0005737
- HAMAP:   MF_00129
- InterPro:   IPR004416
- InterPro:   IPR002218
- InterPro:   IPR020595
- TIGRFAMs:   TIGR00136

Pfam domain/function: PF01134 GIDA

EC number: NA

Molecular weight: Translated: 68830; Mature: 68830

Theoretical pI: Translated: 9.07; Mature: 9.07

Prosite motif: PS01280 GIDA_1; PS01281 GIDA_2

Important sites: BINDING 122-122 BINDING 177-177 BINDING 366-366

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKYDVIVIGGGHAGVEAAAASARLGVPTLLITLKPENLGEMSCNPAIGGIAKGTLVKEI
CCEEEEEEEECCCCCHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCHHHHHH
DALDGLMGYVIDQAGIHYKMLNETRGPAVWGPRAQADRKLYKKAMYQILTNYPNLDILYG
HHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEE
KVEDIEIKSSKIEAVILNNGSKILCQKIILTTGTFLSGLIHIGQKKIPAGRVDEEPSYGL
CEECEEEECCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCH
SNTLKQIGFKLARLKTGTPPRIDGRTIDYSKTILQPGDKIPRPFSELTNIVNVSQINCFI
HHHHHHHHHHHHHHCCCCCCCCCCCEECCHHHHCCCCCCCCCCHHHHHHHEEEEEEEEEE
TKTTSETHDIIRENLDKSAMYSGQIEGIGPRYCPSIEDKIVRFSTKSEHRIFLEPEGLDD
EECCCHHHHHHHHCCCHHHEECCCCCCCCCCCCCCHHHHEEEEECCCCCEEEECCCCCCC
YTIYPNGISTSLPEDVQHKLIKTIPGLENVKVLRPGYAIEYDYVDPREISVTLETKKIAG
EEEECCCCCCCCCHHHHHHHHHHCCCCCCEEEECCCEEEEECCCCCCEEEEEEEEEEEEE
LYLAGQINGTTGYEEAAGQGIIAGINAALAVKDQAPFMLTRANSYIGVMIDDLTTFGTIE
EEEEEEECCCCCCHHHCCCEEEEECCEEEEEECCCCEEEEECCCEEEEEEECCHHCCCCC
PYRMFTSRSEYRLSLRADNSDLRLTELGMNIGVVSEKRKKIFTKKCEDIEKIKSLLNTLS
HHHHHCCCCCEEEEEEECCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
LTTSKLAKMGIQVAQDGTYKTVLDLFKIPNFNVEQAIKIFPMLKETQNKNILQLLYIEAK
HHHHHHHHHCCEEECCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCEEEEEEEHHH
YASYLTRQHADINLFQSEEAQFIPKNIDYFKIPSISLEIQEKLSSHKPTTIGVARRIPGI
HHHHHHHCCCCCEEEECCCCCCCCCCCCEEECCCCCHHHHHHHHCCCCCEEEHHHHCCCC
TPAAITAIIIYLKTKYSDGSST
CHHHHHEEEEEEEEECCCCCCC
>Mature Secondary Structure
MLKYDVIVIGGGHAGVEAAAASARLGVPTLLITLKPENLGEMSCNPAIGGIAKGTLVKEI
CCEEEEEEEECCCCCHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCHHHHHH
DALDGLMGYVIDQAGIHYKMLNETRGPAVWGPRAQADRKLYKKAMYQILTNYPNLDILYG
HHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEE
KVEDIEIKSSKIEAVILNNGSKILCQKIILTTGTFLSGLIHIGQKKIPAGRVDEEPSYGL
CEECEEEECCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCH
SNTLKQIGFKLARLKTGTPPRIDGRTIDYSKTILQPGDKIPRPFSELTNIVNVSQINCFI
HHHHHHHHHHHHHHCCCCCCCCCCCEECCHHHHCCCCCCCCCCHHHHHHHEEEEEEEEEE
TKTTSETHDIIRENLDKSAMYSGQIEGIGPRYCPSIEDKIVRFSTKSEHRIFLEPEGLDD
EECCCHHHHHHHHCCCHHHEECCCCCCCCCCCCCCHHHHEEEEECCCCCEEEECCCCCCC
YTIYPNGISTSLPEDVQHKLIKTIPGLENVKVLRPGYAIEYDYVDPREISVTLETKKIAG
EEEECCCCCCCCCHHHHHHHHHHCCCCCCEEEECCCEEEEECCCCCCEEEEEEEEEEEEE
LYLAGQINGTTGYEEAAGQGIIAGINAALAVKDQAPFMLTRANSYIGVMIDDLTTFGTIE
EEEEEEECCCCCCHHHCCCEEEEECCEEEEEECCCCEEEEECCCEEEEEEECCHHCCCCC
PYRMFTSRSEYRLSLRADNSDLRLTELGMNIGVVSEKRKKIFTKKCEDIEKIKSLLNTLS
HHHHHCCCCCEEEEEEECCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
LTTSKLAKMGIQVAQDGTYKTVLDLFKIPNFNVEQAIKIFPMLKETQNKNILQLLYIEAK
HHHHHHHHHCCEEECCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCEEEEEEEHHH
YASYLTRQHADINLFQSEEAQFIPKNIDYFKIPSISLEIQEKLSSHKPTTIGVARRIPGI
HHHHHHHCCCCCEEEECCCCCCCCCCCCEEECCCCCHHHHHHHHCCCCCEEEHHHHCCCC
TPAAITAIIIYLKTKYSDGSST
CHHHHHEEEEEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA