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The map label for this gene is yjmD [H]

Identifier: 227828827

GI number: 227828827

Start: 2422402

End: 2423355

Strand: Direct

Name: yjmD [H]

Synonym: M1425_2601

Alternate gene names: 227828827

Gene position: 2422402-2423355 (Clockwise)

Preceding gene: 227828826

Following gene: 227828833

Centisome position: 92.85

GC content: 32.08

Gene sequence:

>954_bases
TTGTGGAAGGTATTTTTTCTTAATAGAAATTTCATCAAAGAAAGTGTTAGGGATGAACTTCCTAATTCGTATGAAGTAAA
GGTTTTCCCTAAATACGTAGGAATATGTGGAACTGATAAGAATATCTATTTAGGTAAAAAGCCAGTTAAGGAGCCATTGG
TCTTAGGACACGAAATAGCAGGATTAACGGAAAAAGGGGAAAAGGTAGTTGTTTTTCCAAATTATTGGTGTGGTTATTGT
AATAATTGTAGAAGGGGATTTCATAACAGTTGTAGGAATAAAATCTCAATTGGAGTTAATACTGACGGCGGAATGGCAGA
ATATATTAACGTACCTAAAGATTTTGTGTTCAGAATACCCGATGACTTAAATTTGGAATTAGGAGCTTTAATAGAACCCA
CGGCAGTTGCTGTTGCCGCAGTGAATAAGATAGACAAAGAGATAGGGAAAATAATAATACTAGGGGGCGGTAGTACTGGT
ACTCTAATATCGATAGTAGCTCAAATAAACGGGTTAGAAGCATTTATTATAGAGAAGGATAGAAGAAAAGTGGAAATTCT
TAAAGAGAAAGGGTTTGAAGTAACTAGTGAATTAGAGTTTGATGATAGAATTGTAGTGATAGATACCATAAATAATCAAG
AGAGTATAGCAATAGCTCAGACGATTCTTAAAAACTCAATAGCGAGATCTGAGCTAATAATTACTGGATTAGACGAAGAA
CAGATTAACTTAAATAGAGATATTATAGTTAGGAGTGAGGTAACAATTAAAGGTTCGATTATATATGATAGAGACGACTT
TTTAAATTCTATAAAAATTATTAGTGGAAATAGAGAAAAATTCAATAAAATAGTAGATAAGGTATGTTCAATAGATAGTA
TTAATGAATGTTTCAGAAAATGTGTAATAGAACAACCTAACATAAAAGTCCTCGTAAAAATGGATCCTATTTAG

Upstream 100 bases:

>100_bases
AATATCTTTTAGGCATGGAAATTTTAATCGGGATAGGTCTTATATACTTCATCGGAACAGTATTAGTAACCACCTATAGG
CAAATAAAGGTGAACTAAGA

Downstream 100 bases:

>100_bases
TGACACTCTCTTTAGTTTTCCCACTCATAAAAGAGAATATCATAGCAACTACTATTATTATCGAAGATATGAGAAAAGCA
AAATGTAAGCCATTTACAAA

Product: alcohol dehydrogenase GroES domain protein

Products: L-tagatose phosphate; NADH [C]

Alternate protein names: NA

Number of amino acids: Translated: 317; Mature: 317

Protein sequence:

>317_residues
MWKVFFLNRNFIKESVRDELPNSYEVKVFPKYVGICGTDKNIYLGKKPVKEPLVLGHEIAGLTEKGEKVVVFPNYWCGYC
NNCRRGFHNSCRNKISIGVNTDGGMAEYINVPKDFVFRIPDDLNLELGALIEPTAVAVAAVNKIDKEIGKIIILGGGSTG
TLISIVAQINGLEAFIIEKDRRKVEILKEKGFEVTSELEFDDRIVVIDTINNQESIAIAQTILKNSIARSELIITGLDEE
QINLNRDIIVRSEVTIKGSIIYDRDDFLNSIKIISGNREKFNKIVDKVCSIDSINECFRKCVIEQPNIKVLVKMDPI

Sequences:

>Translated_317_residues
MWKVFFLNRNFIKESVRDELPNSYEVKVFPKYVGICGTDKNIYLGKKPVKEPLVLGHEIAGLTEKGEKVVVFPNYWCGYC
NNCRRGFHNSCRNKISIGVNTDGGMAEYINVPKDFVFRIPDDLNLELGALIEPTAVAVAAVNKIDKEIGKIIILGGGSTG
TLISIVAQINGLEAFIIEKDRRKVEILKEKGFEVTSELEFDDRIVVIDTINNQESIAIAQTILKNSIARSELIITGLDEE
QINLNRDIIVRSEVTIKGSIIYDRDDFLNSIKIISGNREKFNKIVDKVCSIDSINECFRKCVIEQPNIKVLVKMDPI
>Mature_317_residues
MWKVFFLNRNFIKESVRDELPNSYEVKVFPKYVGICGTDKNIYLGKKPVKEPLVLGHEIAGLTEKGEKVVVFPNYWCGYC
NNCRRGFHNSCRNKISIGVNTDGGMAEYINVPKDFVFRIPDDLNLELGALIEPTAVAVAAVNKIDKEIGKIIILGGGSTG
TLISIVAQINGLEAFIIEKDRRKVEILKEKGFEVTSELEFDDRIVVIDTINNQESIAIAQTILKNSIARSELIITGLDEE
QINLNRDIIVRSEVTIKGSIIYDRDDFLNSIKIISGNREKFNKIVDKVCSIDSINECFRKCVIEQPNIKVLVKMDPI

Specific function: Converts Galactitol 1-Phosphate To Tagatose 6-Phosphate. [C]

COG id: COG1063

COG function: function code ER; Threonine dehydrogenase and related Zn-dependent dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the zinc-containing alcohol dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI156627571, Length=325, Percent_Identity=24.3076923076923, Blast_Score=72, Evalue=7e-13,
Organism=Escherichia coli, GI1788407, Length=318, Percent_Identity=26.4150943396226, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1787863, Length=186, Percent_Identity=32.258064516129, Blast_Score=92, Evalue=4e-20,
Organism=Escherichia coli, GI1790045, Length=314, Percent_Identity=25.796178343949, Blast_Score=91, Evalue=8e-20,
Organism=Escherichia coli, GI226510992, Length=309, Percent_Identity=23.9482200647249, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI1788073, Length=161, Percent_Identity=33.5403726708075, Blast_Score=86, Evalue=4e-18,
Organism=Escherichia coli, GI1790718, Length=261, Percent_Identity=24.904214559387, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI87082125, Length=194, Percent_Identity=29.3814432989691, Blast_Score=69, Evalue=6e-13,
Organism=Escherichia coli, GI1788075, Length=323, Percent_Identity=22.2910216718266, Blast_Score=65, Evalue=5e-12,
Organism=Caenorhabditis elegans, GI17562876, Length=313, Percent_Identity=25.2396166134185, Blast_Score=98, Evalue=5e-21,
Organism=Caenorhabditis elegans, GI17562878, Length=173, Percent_Identity=31.7919075144509, Blast_Score=92, Evalue=3e-19,
Organism=Saccharomyces cerevisiae, GI6323099, Length=317, Percent_Identity=25.5520504731861, Blast_Score=89, Evalue=1e-18,
Organism=Saccharomyces cerevisiae, GI6319955, Length=166, Percent_Identity=29.5180722891566, Blast_Score=75, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6322619, Length=166, Percent_Identity=29.5180722891566, Blast_Score=75, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6319258, Length=227, Percent_Identity=27.7533039647577, Blast_Score=72, Evalue=2e-13,
Organism=Drosophila melanogaster, GI17737897, Length=194, Percent_Identity=31.4432989690722, Blast_Score=102, Evalue=5e-22,
Organism=Drosophila melanogaster, GI17137530, Length=194, Percent_Identity=30.9278350515464, Blast_Score=96, Evalue=2e-20,
Organism=Drosophila melanogaster, GI45551930, Length=162, Percent_Identity=25.9259259259259, Blast_Score=68, Evalue=1e-11,
Organism=Drosophila melanogaster, GI221457811, Length=162, Percent_Identity=25.9259259259259, Blast_Score=68, Evalue=1e-11,
Organism=Drosophila melanogaster, GI45550770, Length=162, Percent_Identity=25.9259259259259, Blast_Score=67, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013149
- InterPro:   IPR013154
- InterPro:   IPR002085
- InterPro:   IPR002328
- InterPro:   IPR011032
- InterPro:   IPR016040 [H]

Pfam domain/function: PF08240 ADH_N; PF00107 ADH_zinc_N [H]

EC number: 1.1.1.251 [C]

Molecular weight: Translated: 35657; Mature: 35657

Theoretical pI: Translated: 5.98; Mature: 5.98

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MWKVFFLNRNFIKESVRDELPNSYEVKVFPKYVGICGTDKNIYLGKKPVKEPLVLGHEIA
CEEEEEECHHHHHHHHHHHCCCCEEEEEEEEEEEEECCCCCEEECCCCCCCHHHHCHHHC
GLTEKGEKVVVFPNYWCGYCNNCRRGFHNSCRNKISIGVNTDGGMAEYINVPKDFVFRIP
CCCCCCCEEEEECCCCCHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHCCCHHHEEECC
DDLNLELGALIEPTAVAVAAVNKIDKEIGKIIILGGGSTGTLISIVAQINGLEAFIIEKD
CCCCEEECCEECCHHHHHHHHHHHHHHHCEEEEEECCCCCHHHHHHHHHCCCEEEEEECC
RRKVEILKEKGFEVTSELEFDDRIVVIDTINNQESIAIAQTILKNSIARSELIITGLDEE
CHHHHHHHHCCCCEEEECCCCCEEEEEEECCCCCHHHHHHHHHHHHHCCCEEEEEECCHH
QINLNRDIIVRSEVTIKGSIIYDRDDFLNSIKIISGNREKFNKIVDKVCSIDSINECFRK
HCCCCCEEEEEEEEEEEEEEEECCHHHHCEEEEEECCHHHHHHHHHHHHCCCHHHHHHHH
CVIEQPNIKVLVKMDPI
HCCCCCCEEEEEEECCC
>Mature Secondary Structure
MWKVFFLNRNFIKESVRDELPNSYEVKVFPKYVGICGTDKNIYLGKKPVKEPLVLGHEIA
CEEEEEECHHHHHHHHHHHCCCCEEEEEEEEEEEEECCCCCEEECCCCCCCHHHHCHHHC
GLTEKGEKVVVFPNYWCGYCNNCRRGFHNSCRNKISIGVNTDGGMAEYINVPKDFVFRIP
CCCCCCCEEEEECCCCCHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHCCCHHHEEECC
DDLNLELGALIEPTAVAVAAVNKIDKEIGKIIILGGGSTGTLISIVAQINGLEAFIIEKD
CCCCEEECCEECCHHHHHHHHHHHHHHHCEEEEEECCCCCHHHHHHHHHCCCEEEEEECC
RRKVEILKEKGFEVTSELEFDDRIVVIDTINNQESIAIAQTILKNSIARSELIITGLDEE
CHHHHHHHHCCCCEEEECCCCCEEEEEEECCCCCHHHHHHHHHHHHHCCCEEEEEECCHH
QINLNRDIIVRSEVTIKGSIIYDRDDFLNSIKIISGNREKFNKIVDKVCSIDSINECFRK
HCCCCCEEEEEEEEEEEEEEEECCHHHHCEEEEEECCHHHHHHHHHHHHCCCHHHHHHHH
CVIEQPNIKVLVKMDPI
HCCCCCCEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Zn [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Galactitol-phosphate; NAD+ [C]

Specific reaction: Galactitol-phosphate + NAD+ = L-tagatose phosphate + NADH [C]

General reaction: Oxidation; Reduction [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9579062; 9384377 [H]