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The map label for this gene is cobQ [H]

Identifier: 227828390

GI number: 227828390

Start: 1951018

End: 1952415

Strand: Direct

Name: cobQ [H]

Synonym: M1425_2133

Alternate gene names: 227828390

Gene position: 1951018-1952415 (Clockwise)

Preceding gene: 227828387

Following gene: 227828391

Centisome position: 74.79

GC content: 35.77

Gene sequence:

>1398_bases
GTGTCAATAATAGTAGCCTCAACCATGAGTGATTCAGGTAAGTCATTTGTAACCACTGGACTAGTTAGAATTCTAAATGC
AAAACCACTTAAAATTCAGAATATGTCTTTAAATAGCATTTCAACTTGGGATGGTGGAGAAATAGCCTTCATACAAGCTT
ACCAGGCATGGGGGGCTGGAATAAGACCAGAAAGGCATATGAACCCAATTTTGCTCAAGCCAATGGGTAAGGGCCTTGAA
GTAATCTTTATGGGAAACTCACTTGGTATTATGAGTGGCAATGAGTATTATAATTTAGCAACCAACACGTTGTGGCAAAA
AATCTCACAATTCCTATCAAGTAATGTTGTAATAGAAGCAGCTGGAGGCATAGGAGAACCAAATTTTATTGATAAGGATA
TTACAGCAATAAAAGTAATGAAAGATAAGGGAATTCCAGCAATTCTAGTATTAGATATTGATAGGGGAGGTGCATTTGCG
TCTGGTTATGGCACTTATATGATGCTTCCCCAATCTATCAGAGAGAAATTGAAAGGATTCATTATTAATAAATTCAGAGG
AGATGAAAAGTTTCTGTATGATGCTATAAAATGGCTTGAAGAGAAAACAGGTATGAAATACTTAGGTTATTTGCCTTTTC
TCAGCGAAGTCCCGATAATGCCAGAGGACTCCATGAATATTTACAATTTTGGTGAAGGTGATGTTGAAGTTGCCATAATA
GCCTATCCTTATATGAGCAATTTTAACGAATTGTATGCCTTAGCTAAGTCTAATGCTCATGTATCCTTTGTGAAAAAGCC
TAAAGAAATTGTGAAGGCTGATCTGGTAATTTTACCGGGTAGTAGGAATACTCTAGAATCGTTAAAATGGTTGGTAGAGA
ATGGGTTCGTGGAATATCTAAGGCAAAAGACTAGGGGAATTTTAGGTATTTGCGGTGGTTTTCAAATTTTAGGTAAAAAG
CTTATAGATCCTTATGGAATTGAGGCGGGATATCCTATAGAATATGAGGGTTTAGGAGTATTTGATGTTAACATACACTA
TGGCAAGGCTAAGGTAGTTTCCATTAGTTCTGGTGTTTCAGAATATGGCAAAATAGATGGTTATGAAATTAGAAGGGGAG
TAGTAAAGTATGTGGACGAAAAGCCCTTACTTAAAATAACCGTGAGGAATGGTAAAAAAGTAAGTGTTGAGGATGGGGCA
AGAAAGGATAGTACTATTGGCTTAAGTGTACACGGAAGCTTATTTTCTGAAGGAATGAAAAAATTACTGAAGGAGTTTAA
TATTAATATTTACTCACTATCAATGGTAGACGAGATCCAACAACAAGCAATTCGTGTAGAGCAATTTTTAAGGAAATATT
TACATATTGACGAAATAGTCGAAATTTATAAGCAATGA

Upstream 100 bases:

>100_bases
GCATTGCTCTTTATTGGTATTGCGGTAAAAAACGAGAATAATGCAAGTACTCCTTTTAATCGCATGTGGATTTATTTATT
GGCCAAGTTGATATGATTAA

Downstream 100 bases:

>100_bases
ATTTTTAGTCTTAACTAATGTTTGATCCTGCTTCGTTCGTTAAGGAAATAGGACCACAGCTGAAACAGAAGGTAGGTAAT
GAGAGAGTTTTAGCTGCAGT

Product: cobyric acid synthase CobQ

Products: ADP; phosphate; adenosylcobyric acid; L-glutamate

Alternate protein names: NA

Number of amino acids: Translated: 465; Mature: 464

Protein sequence:

>465_residues
MSIIVASTMSDSGKSFVTTGLVRILNAKPLKIQNMSLNSISTWDGGEIAFIQAYQAWGAGIRPERHMNPILLKPMGKGLE
VIFMGNSLGIMSGNEYYNLATNTLWQKISQFLSSNVVIEAAGGIGEPNFIDKDITAIKVMKDKGIPAILVLDIDRGGAFA
SGYGTYMMLPQSIREKLKGFIINKFRGDEKFLYDAIKWLEEKTGMKYLGYLPFLSEVPIMPEDSMNIYNFGEGDVEVAII
AYPYMSNFNELYALAKSNAHVSFVKKPKEIVKADLVILPGSRNTLESLKWLVENGFVEYLRQKTRGILGICGGFQILGKK
LIDPYGIEAGYPIEYEGLGVFDVNIHYGKAKVVSISSGVSEYGKIDGYEIRRGVVKYVDEKPLLKITVRNGKKVSVEDGA
RKDSTIGLSVHGSLFSEGMKKLLKEFNINIYSLSMVDEIQQQAIRVEQFLRKYLHIDEIVEIYKQ

Sequences:

>Translated_465_residues
MSIIVASTMSDSGKSFVTTGLVRILNAKPLKIQNMSLNSISTWDGGEIAFIQAYQAWGAGIRPERHMNPILLKPMGKGLE
VIFMGNSLGIMSGNEYYNLATNTLWQKISQFLSSNVVIEAAGGIGEPNFIDKDITAIKVMKDKGIPAILVLDIDRGGAFA
SGYGTYMMLPQSIREKLKGFIINKFRGDEKFLYDAIKWLEEKTGMKYLGYLPFLSEVPIMPEDSMNIYNFGEGDVEVAII
AYPYMSNFNELYALAKSNAHVSFVKKPKEIVKADLVILPGSRNTLESLKWLVENGFVEYLRQKTRGILGICGGFQILGKK
LIDPYGIEAGYPIEYEGLGVFDVNIHYGKAKVVSISSGVSEYGKIDGYEIRRGVVKYVDEKPLLKITVRNGKKVSVEDGA
RKDSTIGLSVHGSLFSEGMKKLLKEFNINIYSLSMVDEIQQQAIRVEQFLRKYLHIDEIVEIYKQ
>Mature_464_residues
SIIVASTMSDSGKSFVTTGLVRILNAKPLKIQNMSLNSISTWDGGEIAFIQAYQAWGAGIRPERHMNPILLKPMGKGLEV
IFMGNSLGIMSGNEYYNLATNTLWQKISQFLSSNVVIEAAGGIGEPNFIDKDITAIKVMKDKGIPAILVLDIDRGGAFAS
GYGTYMMLPQSIREKLKGFIINKFRGDEKFLYDAIKWLEEKTGMKYLGYLPFLSEVPIMPEDSMNIYNFGEGDVEVAIIA
YPYMSNFNELYALAKSNAHVSFVKKPKEIVKADLVILPGSRNTLESLKWLVENGFVEYLRQKTRGILGICGGFQILGKKL
IDPYGIEAGYPIEYEGLGVFDVNIHYGKAKVVSISSGVSEYGKIDGYEIRRGVVKYVDEKPLLKITVRNGKKVSVEDGAR
KDSTIGLSVHGSLFSEGMKKLLKEFNINIYSLSMVDEIQQQAIRVEQFLRKYLHIDEIVEIYKQ

Specific function: Catalyzes amidations at positions B, D, E, and G on adenosylcobyrinic A,C-diamide. NH(2) groups are provided by glutamine, and one molecule of ATP is hydrogenolyzed for each amidation [H]

COG id: COG1492

COG function: function code H; Cobyric acid synthase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GATase cobBQ-type domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002586
- InterPro:   IPR017929
- InterPro:   IPR004459
- InterPro:   IPR011698 [H]

Pfam domain/function: PF01656 CbiA; PF07685 GATase_3 [H]

EC number: 6.3.5.10

Molecular weight: Translated: 51895; Mature: 51764

Theoretical pI: Translated: 8.85; Mature: 8.85

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIIVASTMSDSGKSFVTTGLVRILNAKPLKIQNMSLNSISTWDGGEIAFIQAYQAWGAG
CEEEEEECCCCCCCHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCEEEEEEEHHHCCC
IRPERHMNPILLKPMGKGLEVIFMGNSLGIMSGNEYYNLATNTLWQKISQFLSSNVVIEA
CCCCCCCCCEEEEECCCCEEEEEECCEEEECCCCCEEEHHHHHHHHHHHHHHCCCEEEEE
AGGIGEPNFIDKDITAIKVMKDKGIPAILVLDIDRGGAFASGYGTYMMLPQSIREKLKGF
CCCCCCCCCCCCCCEEEEEEECCCCCEEEEEEECCCCCEECCCCEEEECCHHHHHHHHHH
IINKFRGDEKFLYDAIKWLEEKTGMKYLGYLPFLSEVPIMPEDSMNIYNFGEGDVEVAII
EEHCCCCCHHHHHHHHHHHHHHCCCEEEECCCHHHCCCCCCCCCCCEEECCCCCEEEEEE
AYPYMSNFNELYALAKSNAHVSFVKKPKEIVKADLVILPGSRNTLESLKWLVENGFVEYL
EECCCCCHHHHHHHHCCCCEEEECCCCHHHHEECEEEECCCCCHHHHHHHHHHHHHHHHH
RQKTRGILGICGGFQILGKKLIDPYGIEAGYPIEYEGLGVFDVNIHYGKAKVVSISSGVS
HHHCCCEEEECCCHHHHHHHHCCCCCCCCCCCEEECCEEEEEEEEEECCEEEEEECCCHH
EYGKIDGYEIRRGVVKYVDEKPLLKITVRNGKKVSVEDGARKDSTIGLSVHGSLFSEGMK
HHHCCCCHHHHHHHHHHCCCCCEEEEEECCCCEEEECCCCCCCCEEEEEECHHHHHHHHH
KLLKEFNINIYSLSMVDEIQQQAIRVEQFLRKYLHIDEIVEIYKQ
HHHHHHCCCEEEEHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHCC
>Mature Secondary Structure 
SIIVASTMSDSGKSFVTTGLVRILNAKPLKIQNMSLNSISTWDGGEIAFIQAYQAWGAG
EEEEEECCCCCCCHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCEEEEEEEHHHCCC
IRPERHMNPILLKPMGKGLEVIFMGNSLGIMSGNEYYNLATNTLWQKISQFLSSNVVIEA
CCCCCCCCCEEEEECCCCEEEEEECCEEEECCCCCEEEHHHHHHHHHHHHHHCCCEEEEE
AGGIGEPNFIDKDITAIKVMKDKGIPAILVLDIDRGGAFASGYGTYMMLPQSIREKLKGF
CCCCCCCCCCCCCCEEEEEEECCCCCEEEEEEECCCCCEECCCCEEEECCHHHHHHHHHH
IINKFRGDEKFLYDAIKWLEEKTGMKYLGYLPFLSEVPIMPEDSMNIYNFGEGDVEVAII
EEHCCCCCHHHHHHHHHHHHHHCCCEEEECCCHHHCCCCCCCCCCCEEECCCCCEEEEEE
AYPYMSNFNELYALAKSNAHVSFVKKPKEIVKADLVILPGSRNTLESLKWLVENGFVEYL
EECCCCCHHHHHHHHCCCCEEEECCCCHHHHEECEEEECCCCCHHHHHHHHHHHHHHHHH
RQKTRGILGICGGFQILGKKLIDPYGIEAGYPIEYEGLGVFDVNIHYGKAKVVSISSGVS
HHHCCCEEEECCCHHHHHHHHCCCCCCCCCCCEEECCEEEEEEEEEECCEEEEEECCCHH
EYGKIDGYEIRRGVVKYVDEKPLLKITVRNGKKVSVEDGARKDSTIGLSVHGSLFSEGMK
HHHCCCCHHHHHHHHHHCCCCCEEEEEECCCCEEEECCCCCCCCEEEEEECHHHHHHHHH
KLLKEFNINIYSLSMVDEIQQQAIRVEQFLRKYLHIDEIVEIYKQ
HHHHHHCCCEEEEHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; adenosylcobyrinic acid a,c-diamide; L-glutamine; H2O

Specific reaction: 4 ATP + adenosylcobyrinic acid a,c-diamide + 4 L-glutamine + 4 H2O = 4 ADP + 4 phosphate + adenosylcobyric acid + 4 L-glutamate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12552129 [H]