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The map label for this gene is gutB [H]

Identifier: 227828387

GI number: 227828387

Start: 1948365

End: 1949402

Strand: Direct

Name: gutB [H]

Synonym: M1425_2130

Alternate gene names: 227828387

Gene position: 1948365-1949402 (Clockwise)

Preceding gene: 227828382

Following gene: 227828390

Centisome position: 74.68

GC content: 38.63

Gene sequence:

>1038_bases
ATGGTGGAGGAATTCTCACATATGAAAAGCGTAGTTATTGAAAATGGAAAGGTAGCAATAAGAGAGCTGCCATTACCTAA
ATTACAGCAAGGTGACGTATTAGTTAAAATGAAGGCTTGTGGATTGTGTGGTACTGACATAGAGAAGATTTGTGGACAAT
ACACTGCATCGCAACCAATATTAGGCCATGAACCAGCTGGAATAATCCAAGAATCCACGGTTGATTGGTTAAAGCCTGGA
GATAGGGTTTTTGCACACCATCACGTTCCATGTTATGAATGCTACTATTGTAAAAAGGGAAGTCCAACCATGTGTCCATA
TTATAGAAAAACCAACTTAGATCCTGGTGGATTTTCGGAGTACTTCAGAGTTCCAGCCTGGAATGTTGTACGAGGCGGAA
TATTAAAGCTACCAGACAATGTGAGCTTTGAAGAAGGGGCATTTATAGAACCCTTGGCTACCGTTATTAGAGCGCAAAGG
AGAGTAATGATTGATAAGGATGATATAGTTTTTATCGTTGGAGTAGGTCCTATGGGATTATTACACGCAATGATGGCTAA
GGTAAATAATGCGGGAGTCGTTATAGCATCAGATGTAAGTGAGTTCAGAGTCGAATTTGGATATAAGGTAGGTATTGATT
ATTCGCTTAATGTGAAGAAAGTTAGTGTGGTTGATGAAGTTAAGAAATTAACTGATGGAAGAGGTGCTGATGTTACTATA
ATTGCCTCTGGATCTCCCTCTGCAATAATCTCTGGTCTTAATGCTACTAGAAAGGGTGGTAGAGTTTTGTTATTTGGAGT
ACCATATAAGGGGACGATACTTAATTATGACATTAGTGATCTCTTAAATAACGAAATTTCAATAATTCCCAGTAATGCTG
CTGTAGAGGAGGACACAAAGGAAGCACTGTCTATAATAGCTAACAAGAAGATTGATGTAACTAAGCTGATAACACATAGA
TTCCCGTTAGATGAGTTCAATGAGGCCGTAAGGGTAGCTAAGGAGGGAAATGCCATAAAGGTAATAATATATAGTTAG

Upstream 100 bases:

>100_bases
ACGACGAATTCAAGCAAAATAACCAGTGTGAGTAAACCGAAAGTAATAATTAAATCCACAATTAATATAACGTAAGTATT
ACCATATATTCCTTTTTAAA

Downstream 100 bases:

>100_bases
GATGAGGAGAAAGTACAAGATTAAACTTAATTTAAATAATTTTAGCGCATTTTTAATATCTTTTTCGTCTGGCAAATTTC
CATTGCCTATTCTATAGTGA

Product: alcohol dehydrogenase GroES domain protein

Products: NA

Alternate protein names: Glucitol dehydrogenase; L-iditol 2-dehydrogenase [H]

Number of amino acids: Translated: 345; Mature: 345

Protein sequence:

>345_residues
MVEEFSHMKSVVIENGKVAIRELPLPKLQQGDVLVKMKACGLCGTDIEKICGQYTASQPILGHEPAGIIQESTVDWLKPG
DRVFAHHHVPCYECYYCKKGSPTMCPYYRKTNLDPGGFSEYFRVPAWNVVRGGILKLPDNVSFEEGAFIEPLATVIRAQR
RVMIDKDDIVFIVGVGPMGLLHAMMAKVNNAGVVIASDVSEFRVEFGYKVGIDYSLNVKKVSVVDEVKKLTDGRGADVTI
IASGSPSAIISGLNATRKGGRVLLFGVPYKGTILNYDISDLLNNEISIIPSNAAVEEDTKEALSIIANKKIDVTKLITHR
FPLDEFNEAVRVAKEGNAIKVIIYS

Sequences:

>Translated_345_residues
MVEEFSHMKSVVIENGKVAIRELPLPKLQQGDVLVKMKACGLCGTDIEKICGQYTASQPILGHEPAGIIQESTVDWLKPG
DRVFAHHHVPCYECYYCKKGSPTMCPYYRKTNLDPGGFSEYFRVPAWNVVRGGILKLPDNVSFEEGAFIEPLATVIRAQR
RVMIDKDDIVFIVGVGPMGLLHAMMAKVNNAGVVIASDVSEFRVEFGYKVGIDYSLNVKKVSVVDEVKKLTDGRGADVTI
IASGSPSAIISGLNATRKGGRVLLFGVPYKGTILNYDISDLLNNEISIIPSNAAVEEDTKEALSIIANKKIDVTKLITHR
FPLDEFNEAVRVAKEGNAIKVIIYS
>Mature_345_residues
MVEEFSHMKSVVIENGKVAIRELPLPKLQQGDVLVKMKACGLCGTDIEKICGQYTASQPILGHEPAGIIQESTVDWLKPG
DRVFAHHHVPCYECYYCKKGSPTMCPYYRKTNLDPGGFSEYFRVPAWNVVRGGILKLPDNVSFEEGAFIEPLATVIRAQR
RVMIDKDDIVFIVGVGPMGLLHAMMAKVNNAGVVIASDVSEFRVEFGYKVGIDYSLNVKKVSVVDEVKKLTDGRGADVTI
IASGSPSAIISGLNATRKGGRVLLFGVPYKGTILNYDISDLLNNEISIIPSNAAVEEDTKEALSIIANKKIDVTKLITHR
FPLDEFNEAVRVAKEGNAIKVIIYS

Specific function: Reduces glucitol to fructose [H]

COG id: COG1063

COG function: function code ER; Threonine dehydrogenase and related Zn-dependent dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the zinc-containing alcohol dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI156627571, Length=361, Percent_Identity=31.3019390581717, Blast_Score=162, Evalue=4e-40,
Organism=Homo sapiens, GI156523966, Length=356, Percent_Identity=23.5955056179775, Blast_Score=68, Evalue=1e-11,
Organism=Escherichia coli, GI1790045, Length=336, Percent_Identity=29.4642857142857, Blast_Score=152, Evalue=2e-38,
Organism=Escherichia coli, GI87082125, Length=347, Percent_Identity=29.971181556196, Blast_Score=131, Evalue=5e-32,
Organism=Escherichia coli, GI1788407, Length=349, Percent_Identity=28.6532951289398, Blast_Score=117, Evalue=1e-27,
Organism=Escherichia coli, GI226510992, Length=316, Percent_Identity=32.5949367088608, Blast_Score=116, Evalue=2e-27,
Organism=Escherichia coli, GI1788075, Length=340, Percent_Identity=27.9411764705882, Blast_Score=114, Evalue=8e-27,
Organism=Escherichia coli, GI1788073, Length=326, Percent_Identity=26.9938650306748, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI1790718, Length=335, Percent_Identity=28.3582089552239, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1787863, Length=338, Percent_Identity=27.2189349112426, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1786825, Length=367, Percent_Identity=25.0681198910082, Blast_Score=89, Evalue=5e-19,
Organism=Escherichia coli, GI87081918, Length=333, Percent_Identity=28.8288288288288, Blast_Score=87, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17562876, Length=345, Percent_Identity=32.1739130434783, Blast_Score=151, Evalue=5e-37,
Organism=Caenorhabditis elegans, GI17562878, Length=349, Percent_Identity=29.512893982808, Blast_Score=127, Evalue=8e-30,
Organism=Caenorhabditis elegans, GI71988145, Length=306, Percent_Identity=23.8562091503268, Blast_Score=72, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI17562584, Length=308, Percent_Identity=24.6753246753247, Blast_Score=69, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6319955, Length=354, Percent_Identity=30.225988700565, Blast_Score=135, Evalue=9e-33,
Organism=Saccharomyces cerevisiae, GI6322619, Length=354, Percent_Identity=29.9435028248588, Blast_Score=133, Evalue=3e-32,
Organism=Saccharomyces cerevisiae, GI6323099, Length=360, Percent_Identity=28.0555555555556, Blast_Score=130, Evalue=2e-31,
Organism=Saccharomyces cerevisiae, GI6319621, Length=309, Percent_Identity=26.8608414239482, Blast_Score=80, Evalue=3e-16,
Organism=Saccharomyces cerevisiae, GI6323961, Length=306, Percent_Identity=28.4313725490196, Blast_Score=80, Evalue=6e-16,
Organism=Saccharomyces cerevisiae, GI6324486, Length=307, Percent_Identity=28.3387622149837, Blast_Score=75, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6319258, Length=226, Percent_Identity=24.7787610619469, Blast_Score=74, Evalue=4e-14,
Organism=Saccharomyces cerevisiae, GI6319257, Length=340, Percent_Identity=24.7058823529412, Blast_Score=66, Evalue=8e-12,
Organism=Drosophila melanogaster, GI17137530, Length=339, Percent_Identity=29.2035398230088, Blast_Score=141, Evalue=7e-34,
Organism=Drosophila melanogaster, GI17737897, Length=341, Percent_Identity=29.3255131964809, Blast_Score=136, Evalue=2e-32,
Organism=Drosophila melanogaster, GI45550770, Length=330, Percent_Identity=24.5454545454545, Blast_Score=68, Evalue=7e-12,
Organism=Drosophila melanogaster, GI45551930, Length=330, Percent_Identity=24.5454545454545, Blast_Score=68, Evalue=7e-12,
Organism=Drosophila melanogaster, GI221457811, Length=330, Percent_Identity=24.5454545454545, Blast_Score=68, Evalue=9e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013149
- InterPro:   IPR013154
- InterPro:   IPR002085
- InterPro:   IPR002328
- InterPro:   IPR011032
- InterPro:   IPR016040 [H]

Pfam domain/function: PF08240 ADH_N; PF00107 ADH_zinc_N [H]

EC number: =1.1.1.14 [H]

Molecular weight: Translated: 37832; Mature: 37832

Theoretical pI: Translated: 7.14; Mature: 7.14

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVEEFSHMKSVVIENGKVAIRELPLPKLQQGDVLVKMKACGLCGTDIEKICGQYTASQPI
CCCHHHHHHHHEECCCCEEEEECCCCCCCCCCEEEEEEECCCCCCCHHHHHCCCCCCCCC
LGHEPAGIIQESTVDWLKPGDRVFAHHHVPCYECYYCKKGSPTMCPYYRKTNLDPGGFSE
CCCCCCCCHHHCCHHHHCCCCEEEEECCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCHH
YFRVPAWNVVRGGILKLPDNVSFEEGAFIEPLATVIRAQRRVMIDKDDIVFIVGVGPMGL
HHCCCCHHHHCCCEEECCCCCCCCCCCCHHHHHHHHHHHHHEEECCCCEEEEEECCHHHH
LHAMMAKVNNAGVVIASDVSEFRVEFGYKVGIDYSLNVKKVSVVDEVKKLTDGRGADVTI
HHHHHHHCCCCCEEEECCHHHHHHHCCEEECCCEECCEEEEHHHHHHHHHHCCCCCCEEE
IASGSPSAIISGLNATRKGGRVLLFGVPYKGTILNYDISDLLNNEISIIPSNAAVEEDTK
EECCCCHHHHCCCCCCCCCCEEEEEECCCCCEEEECCHHHHCCCCEEEEECCCCCCHHHH
EALSIIANKKIDVTKLITHRFPLDEFNEAVRVAKEGNAIKVIIYS
HHHHHHHCCCCCHHHHHHHCCCHHHHHHHHHHHCCCCEEEEEEEC
>Mature Secondary Structure
MVEEFSHMKSVVIENGKVAIRELPLPKLQQGDVLVKMKACGLCGTDIEKICGQYTASQPI
CCCHHHHHHHHEECCCCEEEEECCCCCCCCCCEEEEEEECCCCCCCHHHHHCCCCCCCCC
LGHEPAGIIQESTVDWLKPGDRVFAHHHVPCYECYYCKKGSPTMCPYYRKTNLDPGGFSE
CCCCCCCCHHHCCHHHHCCCCEEEEECCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCHH
YFRVPAWNVVRGGILKLPDNVSFEEGAFIEPLATVIRAQRRVMIDKDDIVFIVGVGPMGL
HHCCCCHHHHCCCEEECCCCCCCCCCCCHHHHHHHHHHHHHEEECCCCEEEEEECCHHHH
LHAMMAKVNNAGVVIASDVSEFRVEFGYKVGIDYSLNVKKVSVVDEVKKLTDGRGADVTI
HHHHHHHCCCCCEEEECCHHHHHHHCCEEECCCEECCEEEEHHHHHHHHHHCCCCCCEEE
IASGSPSAIISGLNATRKGGRVLLFGVPYKGTILNYDISDLLNNEISIIPSNAAVEEDTK
EECCCCHHHHCCCCCCCCCCEEEEEECCCCCEEEECCHHHHCCCCEEEEECCCCCCHHHH
EALSIIANKKIDVTKLITHRFPLDEFNEAVRVAKEGNAIKVIIYS
HHHHHHHCCCCCHHHHHHHCCCHHHHHHHHHHHCCCCEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1460002; 9384377; 8195086 [H]