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The map label for this gene is yqjC [H]

Identifier: 227826625

GI number: 227826625

Start: 189123

End: 189551

Strand: Direct

Name: yqjC [H]

Synonym: M1425_0216

Alternate gene names: 227826625

Gene position: 189123-189551 (Clockwise)

Preceding gene: 227826623

Following gene: 227826626

Centisome position: 7.25

GC content: 37.06

Gene sequence:

>429_bases
ATGCTGGTTGAAAATCTAGACCATATTGGAATAGTAGTTGAGGATATAGACCAAGCAATTAAGTTCTATCAAAATACTTT
TGGAATGAAATTGATACATCAAGAACTCATTCCAGAGAGAGGGATAAAAGTGGCGTTTTTAGTTGGGGAAGAAGAAAACG
AAACTTCAATAGAGTTATTGGAACCAATCGATCATAACGACATGAATAATACTGTGGCTAAGTTTCTGAAAAATAGGGGC
CAAGGTTTACATCATTTGGCTGTAAAAGTTAGTGATGTTAATAAGGCATTAGAGGAATTGACAGCTAAAGGACTTCAATT
AGTAGATTCCGCCCCGAGAAAGGGGGCAAGAGGACACCTAGTAGCTTTTGTACATCCAAAGAGCGTTATGGGAGTACTAC
TTGAATTGGTACAACCAAAGGAATTATAA

Upstream 100 bases:

>100_bases
AATTCTATCTTCAATATCCATATTATGTTTAAATCCTATAAACAGTATATAACTTTTTCTTTAGGACAAAACTTTTAAGA
TTAAACAATATGAATACGTT

Downstream 100 bases:

>100_bases
ATATCGAGATACACTTGTCGAGGAATGTAAGAAACTTTAAAACTATATTTTCATAATATATAATGATAATCTATGCCCAA
GAGGGAAGAAAAGGATATTT

Product: methylmalonyl-CoA epimerase

Products: glutathione; methylglyoxal

Alternate protein names: NA

Number of amino acids: Translated: 142; Mature: 142

Protein sequence:

>142_residues
MLVENLDHIGIVVEDIDQAIKFYQNTFGMKLIHQELIPERGIKVAFLVGEEENETSIELLEPIDHNDMNNTVAKFLKNRG
QGLHHLAVKVSDVNKALEELTAKGLQLVDSAPRKGARGHLVAFVHPKSVMGVLLELVQPKEL

Sequences:

>Translated_142_residues
MLVENLDHIGIVVEDIDQAIKFYQNTFGMKLIHQELIPERGIKVAFLVGEEENETSIELLEPIDHNDMNNTVAKFLKNRG
QGLHHLAVKVSDVNKALEELTAKGLQLVDSAPRKGARGHLVAFVHPKSVMGVLLELVQPKEL
>Mature_142_residues
MLVENLDHIGIVVEDIDQAIKFYQNTFGMKLIHQELIPERGIKVAFLVGEEENETSIELLEPIDHNDMNNTVAKFLKNRG
QGLHHLAVKVSDVNKALEELTAKGLQLVDSAPRKGARGHLVAFVHPKSVMGVLLELVQPKEL

Specific function: Catalyzes The Conversion Of Hemimercaptal, Formed From Methylglyoxal And Glutathione, To S-Lactoylglutathione. [C]

COG id: COG0346

COG function: function code E; Lactoylglutathione lyase and related lyases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glyoxalase I family [H]

Homologues:

Organism=Homo sapiens, GI188035928, Length=134, Percent_Identity=35.0746268656716, Blast_Score=85, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17506387, Length=138, Percent_Identity=33.3333333333333, Blast_Score=78, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004360
- InterPro:   IPR018146
- InterPro:   IPR017515 [H]

Pfam domain/function: PF00903 Glyoxalase [H]

EC number: 4.4.1.5

Molecular weight: Translated: 15836; Mature: 15836

Theoretical pI: Translated: 5.86; Mature: 5.86

Prosite motif: PS00934 GLYOXALASE_I_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLVENLDHIGIVVEDIDQAIKFYQNTFGMKLIHQELIPERGIKVAFLVGEEENETSIELL
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCHHEEE
EPIDHNDMNNTVAKFLKNRGQGLHHLAVKVSDVNKALEELTAKGLQLVDSAPRKGARGHL
CCCCCCCCHHHHHHHHHHCCCCEEHHHEEHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCE
VAFVHPKSVMGVLLELVQPKEL
EEEECHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MLVENLDHIGIVVEDIDQAIKFYQNTFGMKLIHQELIPERGIKVAFLVGEEENETSIELL
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCHHEEE
EPIDHNDMNNTVAKFLKNRGQGLHHLAVKVSDVNKALEELTAKGLQLVDSAPRKGARGHL
CCCCCCCCHHHHHHHHHHCCCCEEHHHEEHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCE
VAFVHPKSVMGVLLELVQPKEL
EEEECHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): 20280 [C]

Specific activity: NA

Km value (mM): 0.0272 {methylglyoxal/glutathione} [C]

Substrates: (R)-S-lactoylglutathione

Specific reaction: (R)-S-lactoylglutathione = glutathione + methylglyoxal

General reaction: addition of RSH [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969508; 9384377 [H]