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The map label for this gene is fruA-1 [H]

Identifier: 227081955

GI number: 227081955

Start: 1903790

End: 1905691

Strand: Direct

Name: fruA-1 [H]

Synonym: VCM66_1749

Alternate gene names: 227081955

Gene position: 1903790-1905691 (Clockwise)

Preceding gene: 227081953

Following gene: 227081956

Centisome position: 65.82

GC content: 50.37

Gene sequence:

>1902_bases
ATGATCAACCAATTGATTAATGCCGATTTAATTCAGCTTGATCTGCAAGCGAATTCCAAACAAGCCGTATTTGAAGAACT
GATAAATATTCTACATGCCCAAGGGCGAATTTCAGATAAAGCGGCATTCTTAAAAGATATTCAAGCTCGTGAAGAGTTAG
GTAATACCGGATTTGAAGATGGTGTCGCTATTCCGCATGCTAAAAGTGCAGCGGTGACTCAACCAGCTGTTGCAATTGGT
GTCAGCCGAGAGGGTATCGACTATGGCGCAGAAGATGGTTTGCCTTCCAAGCTTTTTTTCATGATCGCCTCCCCCGATGG
CGGCGATAACCACCACATTGAAGTGCTGGCCGAACTCTCTTCCAAACTGATTGAAGAAGGTTTTATCGAGGCTTTTCTCA
ACACCAAAAACTCAGAGCAAGCACTAGAATTACTGCTGAAAAAATCCGCGCCTACACCTCTTGCAAACCAAGCAGAAAGC
AAAGGGCTGATTATCGGTGTTACCGGTTGCCCGGCAGGTATTGCTCACACTTACCTTGCGGCTGAAGCACTGGAAAAAGG
CGCGGCGGCTTTGGGTTTTGAGATCAAAGTGGAAACCAATGGCTCGATTGGGGTGAAAAATAGCCCAAGTGCGGAAGAAA
TTGAACGCGCTGAAGCGATTGTGGTCGCCTGTGATAAACAAGTCGACATGGCACGCTTTGCCGGCAAACGCCTCATCAAA
ACAAATGTGAAAGCGCCAATTCGTGATGCACAAAAGCTGATTAATGAAGCGCTAAGGGCGCCAACTTATCAAGCTGAAAC
GGTTAAGGCTCAATCGGTGGCAAACAAAGCCTCACAAGCTCGCTCGGATTTGTATCGCTTTTTGATGAATGGCGTTTCGC
ACATGATCCCATTTGTGGTAACTGGCGGGCTTTTAATTGCACTGGCTCTCGCGGTGGGTGGTGAGCCAACTGAAGCGGGT
ATGGCCATTCCAGCGGGCAGTATGTGGAACCAAATCCTTGAAGTGGGCGTAGTGGCCTTTACCTTGATGATTCCCATCCT
TGCCGGCTACATCGCCTACGCCATTGCCGATCGCCCTGCGCTCTCTCCCGGTTTGATTGGTGGCTGGATTGCCAATAACG
GCTCTTTTTATGGTGCTGACGCAGGTACAGGGTTTATCGGTGCCATCATTGCGGGTCTCTTGGTCGGTTATTTCGTGAAG
TGGATTACTTCCATTAACTATCACAAGTTTATCCAACCCCTAGTGCCGATCATGATCGCGCCGATCACGGCATCGCTGTT
CATCGCTGGTCTGTTCATTTTCGTTATTGGCGCTCCCATTGCGAGCCTGATGGATGGCCTCACTGCTCTACTGACTAGCA
TGAGTTCCGGCAACGTTATTCTGCTCGGCATCGTACTCGGTGGCATGGCAGGCTTTGATATGGGAGGCCCATTCAACAAA
GTCGCTTTCCTATTCTCTGTAGGCATGATTGCCAGCGGCCAAACTCAATTTATGGGTGCGATGGCCTGTGCGATTCCCGT
TGCACCACTTGGTATGGCACTCGCAACAGCGATGGGTCGTAAGCTCGAACTGTTTGAGTCTTCTGAGCTTGAAGCAGGTA
AAGCCGCAGGCGCAATGGGCTTAGTCGGTATCTCTGAAGGCGCGATTCCTTTTGCGGCGCAAGACCCAATGTCAGTCATT
CCAGCCAACGTGTTGGGCTCTATGGTTGCCGCTGTCATGGCATTCTCTTTTGGCATCACCAACAGTGTGGCTCACGGTGG
CCCCGTAGTCGCCCTGCTTGGCGCAATGAACTACCCACTGCTTGCCCTACTTTGTATGGCGGCTGGTGCGGGTGTCACCG
CCATCACCTGTGTGACGCTCAAAAAAATCCGCTCACAAAAGTTTGAAGCCGCTGCGGCTTAA

Upstream 100 bases:

>100_bases
GTTACAATCCTCTAGTGAATGTTTTATTTCTGCACTAATAAGAAAAATAAAAAAATTAAAGAATAACTCTGACATAAAAA
TAAAATGTACAGAGGTCTCT

Downstream 100 bases:

>100_bases
TTCTCCCTTCCTCGTTATGGCTTGAGGTTCCACTCAAGCCATCTTTCTAACGGTGACGACAATGACTCAGATTCCGACTC
AGGATGCAGCCCTACCAGAG

Product: PTS system, fructose-specific IIABC component

Products: NA

Alternate protein names: EIIBCA-Man; EII-Man; Mannose-specific phosphotransferase enzyme IIB component; PTS system mannose-specific EIIB component; Mannose permease IIC component; PTS system mannose-specific EIIC component; Mannose-specific phosphotransferase enzyme IIA component; PTS system mannose-specific EIIA component [H]

Number of amino acids: Translated: 633; Mature: 633

Protein sequence:

>633_residues
MINQLINADLIQLDLQANSKQAVFEELINILHAQGRISDKAAFLKDIQAREELGNTGFEDGVAIPHAKSAAVTQPAVAIG
VSREGIDYGAEDGLPSKLFFMIASPDGGDNHHIEVLAELSSKLIEEGFIEAFLNTKNSEQALELLLKKSAPTPLANQAES
KGLIIGVTGCPAGIAHTYLAAEALEKGAAALGFEIKVETNGSIGVKNSPSAEEIERAEAIVVACDKQVDMARFAGKRLIK
TNVKAPIRDAQKLINEALRAPTYQAETVKAQSVANKASQARSDLYRFLMNGVSHMIPFVVTGGLLIALALAVGGEPTEAG
MAIPAGSMWNQILEVGVVAFTLMIPILAGYIAYAIADRPALSPGLIGGWIANNGSFYGADAGTGFIGAIIAGLLVGYFVK
WITSINYHKFIQPLVPIMIAPITASLFIAGLFIFVIGAPIASLMDGLTALLTSMSSGNVILLGIVLGGMAGFDMGGPFNK
VAFLFSVGMIASGQTQFMGAMACAIPVAPLGMALATAMGRKLELFESSELEAGKAAGAMGLVGISEGAIPFAAQDPMSVI
PANVLGSMVAAVMAFSFGITNSVAHGGPVVALLGAMNYPLLALLCMAAGAGVTAITCVTLKKIRSQKFEAAAA

Sequences:

>Translated_633_residues
MINQLINADLIQLDLQANSKQAVFEELINILHAQGRISDKAAFLKDIQAREELGNTGFEDGVAIPHAKSAAVTQPAVAIG
VSREGIDYGAEDGLPSKLFFMIASPDGGDNHHIEVLAELSSKLIEEGFIEAFLNTKNSEQALELLLKKSAPTPLANQAES
KGLIIGVTGCPAGIAHTYLAAEALEKGAAALGFEIKVETNGSIGVKNSPSAEEIERAEAIVVACDKQVDMARFAGKRLIK
TNVKAPIRDAQKLINEALRAPTYQAETVKAQSVANKASQARSDLYRFLMNGVSHMIPFVVTGGLLIALALAVGGEPTEAG
MAIPAGSMWNQILEVGVVAFTLMIPILAGYIAYAIADRPALSPGLIGGWIANNGSFYGADAGTGFIGAIIAGLLVGYFVK
WITSINYHKFIQPLVPIMIAPITASLFIAGLFIFVIGAPIASLMDGLTALLTSMSSGNVILLGIVLGGMAGFDMGGPFNK
VAFLFSVGMIASGQTQFMGAMACAIPVAPLGMALATAMGRKLELFESSELEAGKAAGAMGLVGISEGAIPFAAQDPMSVI
PANVLGSMVAAVMAFSFGITNSVAHGGPVVALLGAMNYPLLALLCMAAGAGVTAITCVTLKKIRSQKFEAAAA
>Mature_633_residues
MINQLINADLIQLDLQANSKQAVFEELINILHAQGRISDKAAFLKDIQAREELGNTGFEDGVAIPHAKSAAVTQPAVAIG
VSREGIDYGAEDGLPSKLFFMIASPDGGDNHHIEVLAELSSKLIEEGFIEAFLNTKNSEQALELLLKKSAPTPLANQAES
KGLIIGVTGCPAGIAHTYLAAEALEKGAAALGFEIKVETNGSIGVKNSPSAEEIERAEAIVVACDKQVDMARFAGKRLIK
TNVKAPIRDAQKLINEALRAPTYQAETVKAQSVANKASQARSDLYRFLMNGVSHMIPFVVTGGLLIALALAVGGEPTEAG
MAIPAGSMWNQILEVGVVAFTLMIPILAGYIAYAIADRPALSPGLIGGWIANNGSFYGADAGTGFIGAIIAGLLVGYFVK
WITSINYHKFIQPLVPIMIAPITASLFIAGLFIFVIGAPIASLMDGLTALLTSMSSGNVILLGIVLGGMAGFDMGGPFNK
VAFLFSVGMIASGQTQFMGAMACAIPVAPLGMALATAMGRKLELFESSELEAGKAAGAMGLVGISEGAIPFAAQDPMSVI
PANVLGSMVAAVMAFSFGITNSVAHGGPVVALLGAMNYPLLALLCMAAGAGVTAITCVTLKKIRSQKFEAAAA

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This

COG id: COG1299

COG function: function code G; Phosphotransferase system, fructose-specific IIC component

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIC type-2 domain [H]

Homologues:

Organism=Escherichia coli, GI1788492, Length=462, Percent_Identity=40.4761904761905, Blast_Score=322, Evalue=6e-89,
Organism=Escherichia coli, GI1786951, Length=587, Percent_Identity=32.5383304940375, Blast_Score=293, Evalue=3e-80,
Organism=Escherichia coli, GI87082348, Length=481, Percent_Identity=34.5114345114345, Blast_Score=242, Evalue=5e-65,
Organism=Escherichia coli, GI1790386, Length=319, Percent_Identity=42.3197492163009, Blast_Score=229, Evalue=4e-61,
Organism=Escherichia coli, GI1788729, Length=402, Percent_Identity=27.363184079602, Blast_Score=106, Evalue=4e-24,
Organism=Escherichia coli, GI1790387, Length=94, Percent_Identity=43.6170212765958, Blast_Score=89, Evalue=9e-19,
Organism=Escherichia coli, GI2367327, Length=140, Percent_Identity=33.5714285714286, Blast_Score=87, Evalue=3e-18,
Organism=Escherichia coli, GI1788730, Length=99, Percent_Identity=37.3737373737374, Blast_Score=65, Evalue=1e-11,
Organism=Escherichia coli, GI1790390, Length=102, Percent_Identity=33.3333333333333, Blast_Score=64, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016152
- InterPro:   IPR002178
- InterPro:   IPR013011
- InterPro:   IPR003501
- InterPro:   IPR003352
- InterPro:   IPR013014
- InterPro:   IPR004715
- InterPro:   IPR003353
- InterPro:   IPR006327 [H]

Pfam domain/function: PF00359 PTS_EIIA_2; PF02378 PTS_EIIC; PF02302 PTS_IIB [H]

EC number: =2.7.1.69 [H]

Molecular weight: Translated: 65618; Mature: 65618

Theoretical pI: Translated: 5.20; Mature: 5.20

Prosite motif: PS51094 PTS_EIIA_TYPE_2 ; PS51099 PTS_EIIB_TYPE_2 ; PS51104 PTS_EIIC_TYPE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MINQLINADLIQLDLQANSKQAVFEELINILHAQGRISDKAAFLKDIQAREELGNTGFED
CCCCHHCCCEEEEEECCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCC
GVAIPHAKSAAVTQPAVAIGVSREGIDYGAEDGLPSKLFFMIASPDGGDNHHIEVLAELS
CCCCCCCCCCCCCCCHHEEECCCCCCCCCCCCCCCCEEEEEEECCCCCCCHHHHHHHHHH
SKLIEEGFIEAFLNTKNSEQALELLLKKSAPTPLANQAESKGLIIGVTGCPAGIAHTYLA
HHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCCCCCEEEEECCCCHHHHHHHHH
AEALEKGAAALGFEIKVETNGSIGVKNSPSAEEIERAEAIVVACDKQVDMARFAGKRLIK
HHHHHCCCHHCCEEEEEECCCCCCCCCCCCHHHHHHHCEEEEEECCCHHHHHHHCCHHHH
TNVKAPIRDAQKLINEALRAPTYQAETVKAQSVANKASQARSDLYRFLMNGVSHMIPFVV
HCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TGGLLIALALAVGGEPTEAGMAIPAGSMWNQILEVGVVAFTLMIPILAGYIAYAIADRPA
HHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
LSPGLIGGWIANNGSFYGADAGTGFIGAIIAGLLVGYFVKWITSINYHKFIQPLVPIMIA
CCCCCEECEEECCCCEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
PITASLFIAGLFIFVIGAPIASLMDGLTALLTSMSSGNVILLGIVLGGMAGFDMGGPFNK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEHHHHHCCCCCCCCCCHHH
VAFLFSVGMIASGQTQFMGAMACAIPVAPLGMALATAMGRKLELFESSELEAGKAAGAMG
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEHHCCCCCCCCHHCCCEE
LVGISEGAIPFAAQDPMSVIPANVLGSMVAAVMAFSFGITNSVAHGGPVVALLGAMNYPL
EEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCHHH
LALLCMAAGAGVTAITCVTLKKIRSQKFEAAAA
HHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MINQLINADLIQLDLQANSKQAVFEELINILHAQGRISDKAAFLKDIQAREELGNTGFED
CCCCHHCCCEEEEEECCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCC
GVAIPHAKSAAVTQPAVAIGVSREGIDYGAEDGLPSKLFFMIASPDGGDNHHIEVLAELS
CCCCCCCCCCCCCCCHHEEECCCCCCCCCCCCCCCCEEEEEEECCCCCCCHHHHHHHHHH
SKLIEEGFIEAFLNTKNSEQALELLLKKSAPTPLANQAESKGLIIGVTGCPAGIAHTYLA
HHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCCCCCEEEEECCCCHHHHHHHHH
AEALEKGAAALGFEIKVETNGSIGVKNSPSAEEIERAEAIVVACDKQVDMARFAGKRLIK
HHHHHCCCHHCCEEEEEECCCCCCCCCCCCHHHHHHHCEEEEEECCCHHHHHHHCCHHHH
TNVKAPIRDAQKLINEALRAPTYQAETVKAQSVANKASQARSDLYRFLMNGVSHMIPFVV
HCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TGGLLIALALAVGGEPTEAGMAIPAGSMWNQILEVGVVAFTLMIPILAGYIAYAIADRPA
HHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
LSPGLIGGWIANNGSFYGADAGTGFIGAIIAGLLVGYFVKWITSINYHKFIQPLVPIMIA
CCCCCEECEEECCCCEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
PITASLFIAGLFIFVIGAPIASLMDGLTALLTSMSSGNVILLGIVLGGMAGFDMGGPFNK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEHHHHHCCCCCCCCCCHHH
VAFLFSVGMIASGQTQFMGAMACAIPVAPLGMALATAMGRKLELFESSELEAGKAAGAMG
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEHHCCCCCCCCHHCCCEE
LVGISEGAIPFAAQDPMSVIPANVLGSMVAAVMAFSFGITNSVAHGGPVVALLGAMNYPL
EEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCHHH
LALLCMAAGAGVTAITCVTLKKIRSQKFEAAAA
HHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]