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The map label for this gene is manA-2 [H]

Identifier: 227081956

GI number: 227081956

Start: 1905807

End: 1906973

Strand: Direct

Name: manA-2 [H]

Synonym: VCM66_1750

Alternate gene names: 227081956

Gene position: 1905807-1906973 (Clockwise)

Preceding gene: 227081955

Following gene: 227081959

Centisome position: 65.89

GC content: 46.1

Gene sequence:

>1167_bases
ATGACCAACCCTATCCAAAACTACGCATGGGGCAGTAAAACCGCTTTGCAGCAGTTGTTTGAACTCGAAAACCCAAACGA
TGACCCCCAAGCAGAGCTATGGATGGGCGCTCATCCTAATGGCTGCTCCGGTATAGCCATTAACGGCAAAATCGTTCAAC
TTTCCGACTTTATTGCGCAAAATCCAAACTTGATTTTGGGTGAATATACCGCGCGGCAGTTCGGCGAACTCCCTTATTTG
TTTAAAATTTTGGCGGCGGAAAAAGCCCTCTCCATTCAGGTCCATCCGAACAAACAGCAAGCGGAACGAGGTTTCGCCCA
AGAGGAACAATTAGGCATTGCGCTCACGGCTGCTCAACGAAATTACAAAGACCCCAATCACAAACCCGAATTGGTTTATG
CGCTCACCGAGTACCAAGCAATGAACGGCTTTCGTGCCAATCAGGAAATCCTTAACTACTTTATTGAGTTATCGATAGAT
GAGATCCAGCCTCTCGTTAACGTATTCCAATCAAATCCGACAGAACAAGGGCTCAGAGACTTTTTCTCAGGGTTACTCTC
ATTACAGGGAGAAGCCAAAAACCGTGCGCTTGAAGCTTTGATGGCTCAAGCTAAGCAGATTGATCTGCCTCTTTTCCAGC
TGATCGTCGAATTGGAAAACCAATATCCCAATGACATTGGTTTATTCGCTCCTCTGATGCTCAATGTGATTACCTTACAA
CCAGGTGAAGCGATGTTTTTAGACGCAGAAACGCCGCACGCGTATTTACACGGAACAGGCCTTGAGATTATGGCCAACTC
CGACAACGTGTTGCGCGCGGGTTTAACACCAAAATACATGGATATTGATGAATTGGTGGCATGTACTCAGTTCAAACATA
AGCCACTTGAACAACTACGCCTTAAGCCTGAAGTCATTGAAGGGTGTGAACAATATCCGATCCCAGTTGCCGATTTTAAA
TTTGCCATCATCCCACCTACGACTCAGCAGACTATCGGCGTTAAGAGTGCAGAAGTTGTGCTGCCTCTCGATTCTTCAAT
GATATTGCGACATGCAAATGGTGAGCAATGCCTTGTACGAAAAGGACAATCTGTCTTTATTCCGGCTTATGCTGAGCAGT
ACACCATTGAGTGTAATGGTCGTGTGGCAAGAGCTTTTAGCGCCTAG

Upstream 100 bases:

>100_bases
TATGGCTTGAGGTTCCACTCAAGCCATCTTTCTAACGGTGACGACAATGACTCAGATTCCGACTCAGGATGCAGCCCTAC
CAGAGGCAGTATTCTTCCCG

Downstream 100 bases:

>100_bases
CATTAGCCCAAAAGGCCAGTTTTACTTATTCCCAAGATTCGATTGCTCGATTTAGGGTAATAACTGAAATTGGCCAAATT
AAATGGAGGGGCAGTTAACA

Product: mannose-6-phosphate isomerase

Products: NA

Alternate protein names: Phosphohexomutase; Phosphomannose isomerase; PMI [H]

Number of amino acids: Translated: 388; Mature: 387

Protein sequence:

>388_residues
MTNPIQNYAWGSKTALQQLFELENPNDDPQAELWMGAHPNGCSGIAINGKIVQLSDFIAQNPNLILGEYTARQFGELPYL
FKILAAEKALSIQVHPNKQQAERGFAQEEQLGIALTAAQRNYKDPNHKPELVYALTEYQAMNGFRANQEILNYFIELSID
EIQPLVNVFQSNPTEQGLRDFFSGLLSLQGEAKNRALEALMAQAKQIDLPLFQLIVELENQYPNDIGLFAPLMLNVITLQ
PGEAMFLDAETPHAYLHGTGLEIMANSDNVLRAGLTPKYMDIDELVACTQFKHKPLEQLRLKPEVIEGCEQYPIPVADFK
FAIIPPTTQQTIGVKSAEVVLPLDSSMILRHANGEQCLVRKGQSVFIPAYAEQYTIECNGRVARAFSA

Sequences:

>Translated_388_residues
MTNPIQNYAWGSKTALQQLFELENPNDDPQAELWMGAHPNGCSGIAINGKIVQLSDFIAQNPNLILGEYTARQFGELPYL
FKILAAEKALSIQVHPNKQQAERGFAQEEQLGIALTAAQRNYKDPNHKPELVYALTEYQAMNGFRANQEILNYFIELSID
EIQPLVNVFQSNPTEQGLRDFFSGLLSLQGEAKNRALEALMAQAKQIDLPLFQLIVELENQYPNDIGLFAPLMLNVITLQ
PGEAMFLDAETPHAYLHGTGLEIMANSDNVLRAGLTPKYMDIDELVACTQFKHKPLEQLRLKPEVIEGCEQYPIPVADFK
FAIIPPTTQQTIGVKSAEVVLPLDSSMILRHANGEQCLVRKGQSVFIPAYAEQYTIECNGRVARAFSA
>Mature_387_residues
TNPIQNYAWGSKTALQQLFELENPNDDPQAELWMGAHPNGCSGIAINGKIVQLSDFIAQNPNLILGEYTARQFGELPYLF
KILAAEKALSIQVHPNKQQAERGFAQEEQLGIALTAAQRNYKDPNHKPELVYALTEYQAMNGFRANQEILNYFIELSIDE
IQPLVNVFQSNPTEQGLRDFFSGLLSLQGEAKNRALEALMAQAKQIDLPLFQLIVELENQYPNDIGLFAPLMLNVITLQP
GEAMFLDAETPHAYLHGTGLEIMANSDNVLRAGLTPKYMDIDELVACTQFKHKPLEQLRLKPEVIEGCEQYPIPVADFKF
AIIPPTTQQTIGVKSAEVVLPLDSSMILRHANGEQCLVRKGQSVFIPAYAEQYTIECNGRVARAFSA

Specific function: Involved in the conversion of glucose to GDP-L-fucose, which can be converted to L-fucose, a capsular polysaccharide [H]

COG id: COG1482

COG function: function code G; Phosphomannose isomerase

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mannose-6-phosphate isomerase type 1 family [H]

Homologues:

Organism=Homo sapiens, GI4505235, Length=388, Percent_Identity=35.8247422680412, Blast_Score=192, Evalue=6e-49,
Organism=Escherichia coli, GI1787899, Length=390, Percent_Identity=48.4615384615385, Blast_Score=353, Evalue=1e-98,
Organism=Caenorhabditis elegans, GI71997620, Length=305, Percent_Identity=36.0655737704918, Blast_Score=172, Evalue=3e-43,
Organism=Caenorhabditis elegans, GI17557650, Length=417, Percent_Identity=29.2565947242206, Blast_Score=161, Evalue=5e-40,
Organism=Saccharomyces cerevisiae, GI6320839, Length=404, Percent_Identity=33.1683168316832, Blast_Score=172, Evalue=1e-43,
Organism=Drosophila melanogaster, GI21356061, Length=406, Percent_Identity=33.2512315270936, Blast_Score=182, Evalue=2e-46,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011051
- InterPro:   IPR001250
- InterPro:   IPR016305
- InterPro:   IPR018050
- InterPro:   IPR014710 [H]

Pfam domain/function: PF01238 PMI_typeI [H]

EC number: =5.3.1.8 [H]

Molecular weight: Translated: 43276; Mature: 43145

Theoretical pI: Translated: 4.59; Mature: 4.59

Prosite motif: PS00965 PMI_I_1 ; PS00966 PMI_I_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNPIQNYAWGSKTALQQLFELENPNDDPQAELWMGAHPNGCSGIAINGKIVQLSDFIAQ
CCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCEEEECCCCCCCCEEEECCEEEEEHHHHHC
NPNLILGEYTARQFGELPYLFKILAAEKALSIQVHPNKQQAERGFAQEEQLGIALTAAQR
CCCEEEECCHHHHHCCCHHHHHHHHHCCEEEEEECCCHHHHHCCCCHHHHCCEEEEECCC
NYKDPNHKPELVYALTEYQAMNGFRANQEILNYFIELSIDEIQPLVNVFQSNPTEQGLRD
CCCCCCCCCCEEEEEHHHHHHCCCCCCHHHHHHHHHCCHHHHHHHHHHHHCCCHHHHHHH
FFSGLLSLQGEAKNRALEALMAQAKQIDLPLFQLIVELENQYPNDIGLFAPLMLNVITLQ
HHHHHHHCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCHHHHHHHHHEEEEEC
PGEAMFLDAETPHAYLHGTGLEIMANSDNVLRAGLTPKYMDIDELVACTQFKHKPLEQLR
CCCEEEEECCCCCEEEECCCEEEEECCCCEEEECCCCCCCCHHHHHHHHHHCCCCHHHHC
LKPEVIEGCEQYPIPVADFKFAIIPPTTQQTIGVKSAEVVLPLDSSMILRHANGEQCLVR
CCCHHHHCHHHCCCCHHCCEEEEECCCCCHHCCCCCCEEEEECCCCEEEEECCCCCHHHC
KGQSVFIPAYAEQYTIECNGRVARAFSA
CCCEEEEEECCCEEEEEECCEEEEECCC
>Mature Secondary Structure 
TNPIQNYAWGSKTALQQLFELENPNDDPQAELWMGAHPNGCSGIAINGKIVQLSDFIAQ
CCCCCCCCCCCHHHHHHHHHHCCCCCCCCCEEEECCCCCCCCEEEECCEEEEEHHHHHC
NPNLILGEYTARQFGELPYLFKILAAEKALSIQVHPNKQQAERGFAQEEQLGIALTAAQR
CCCEEEECCHHHHHCCCHHHHHHHHHCCEEEEEECCCHHHHHCCCCHHHHCCEEEEECCC
NYKDPNHKPELVYALTEYQAMNGFRANQEILNYFIELSIDEIQPLVNVFQSNPTEQGLRD
CCCCCCCCCCEEEEEHHHHHHCCCCCCHHHHHHHHHCCHHHHHHHHHHHHCCCHHHHHHH
FFSGLLSLQGEAKNRALEALMAQAKQIDLPLFQLIVELENQYPNDIGLFAPLMLNVITLQ
HHHHHHHCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCHHHHHHHHHEEEEEC
PGEAMFLDAETPHAYLHGTGLEIMANSDNVLRAGLTPKYMDIDELVACTQFKHKPLEQLR
CCCEEEEECCCCCEEEECCCEEEEECCCCEEEECCCCCCCCHHHHHHHHHHCCCCHHHHC
LKPEVIEGCEQYPIPVADFKFAIIPPTTQQTIGVKSAEVVLPLDSSMILRHANGEQCLVR
CCCHHHHCHHHCCCCHHCCEEEEECCCCCHHCCCCCCEEEEECCCCEEEEECCCCCHHHC
KGQSVFIPAYAEQYTIECNGRVARAFSA
CCCEEEEEECCCEEEEEECCEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 6397402; 9097039; 9278503 [H]