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The map label for this gene is frwB [H]

Identifier: 227081952

GI number: 227081952

Start: 1901500

End: 1901799

Strand: Direct

Name: frwB [H]

Synonym: VCM66_1746

Alternate gene names: 227081952

Gene position: 1901500-1901799 (Clockwise)

Preceding gene: 227081950

Following gene: 227081953

Centisome position: 65.74

GC content: 49.33

Gene sequence:

>300_bases
ATGAAAATTGTTGCGGTCACCGCCTGCCCAACGGGCATTGCTCATACTTATATGGCGGCCGATGCTTTGACCAAAGCCGC
ACCAAAGTACAATGTGCAGATCAAAGTGGAAACTCAAGGTGCGATGGGTATCGAGAATCCTCTCACACCTTATGACCTCT
CCCATGCCGATAAGGTGTTGATCGTCTCCGATATCGACATCGAACAGCCTGCACGATTTGAAGGGATGAAGGTGGTGAAG
CTCTCGATCGAAGAAGTGCTACTCAATGTCGATAAAGTCTTTCTCGTCCACTGTCGCTGA

Upstream 100 bases:

>100_bases
TAACATTCGATTTGAACTGTGAACTGGGTCTTGGCCGAAAACGGAGTATTTGCCAAGCGCTATGTTACTATCGCCGCAAA
AATAACAATGAGGGATTTCC

Downstream 100 bases:

>100_bases
TCCTTGGCATGATGCATGAATTCCAAGTCACCTTCTTGGTGAATGATGTGAATGCCAGCGTCCATGTTGCCCAACCGCTG
TCTCGCGTTGCGCGTAAGTT

Product: PTS system, fructose-specific IIB component

Products: NA

Alternate protein names: PTS system fructose-like EIIB component 2 [H]

Number of amino acids: Translated: 99; Mature: 99

Protein sequence:

>99_residues
MKIVAVTACPTGIAHTYMAADALTKAAPKYNVQIKVETQGAMGIENPLTPYDLSHADKVLIVSDIDIEQPARFEGMKVVK
LSIEEVLLNVDKVFLVHCR

Sequences:

>Translated_99_residues
MKIVAVTACPTGIAHTYMAADALTKAAPKYNVQIKVETQGAMGIENPLTPYDLSHADKVLIVSDIDIEQPARFEGMKVVK
LSIEEVLLNVDKVFLVHCR
>Mature_99_residues
MKIVAVTACPTGIAHTYMAADALTKAAPKYNVQIKVETQGAMGIENPLTPYDLSHADKVLIVSDIDIEQPARFEGMKVVK
LSIEEVLLNVDKVFLVHCR

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane [H]

COG id: COG1445

COG function: function code G; Phosphotransferase system fructose-specific component IIB

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIB type-2 domain [H]

Homologues:

Organism=Escherichia coli, GI1790387, Length=93, Percent_Identity=47.3118279569892, Blast_Score=97, Evalue=2e-22,
Organism=Escherichia coli, GI87082348, Length=96, Percent_Identity=43.75, Blast_Score=85, Evalue=8e-19,
Organism=Escherichia coli, GI1790390, Length=92, Percent_Identity=42.3913043478261, Blast_Score=78, Evalue=1e-16,
Organism=Escherichia coli, GI1788492, Length=81, Percent_Identity=44.4444444444444, Blast_Score=77, Evalue=2e-16,
Organism=Escherichia coli, GI1786951, Length=83, Percent_Identity=45.7831325301205, Blast_Score=76, Evalue=3e-16,
Organism=Escherichia coli, GI1788730, Length=88, Percent_Identity=39.7727272727273, Blast_Score=74, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013011
- InterPro:   IPR003501
- InterPro:   IPR003353 [H]

Pfam domain/function: PF02302 PTS_IIB [H]

EC number: =2.7.1.69 [H]

Molecular weight: Translated: 10838; Mature: 10838

Theoretical pI: Translated: 6.24; Mature: 6.24

Prosite motif: PS51099 PTS_EIIB_TYPE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
6.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIVAVTACPTGIAHTYMAADALTKAAPKYNVQIKVETQGAMGIENPLTPYDLSHADKVL
CEEEEEEECCCCHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCEEE
IVSDIDIEQPARFEGMKVVKLSIEEVLLNVDKVFLVHCR
EEECCCCCCCCCCCCEEEEEEEHHHHHHCCCEEEEEEEC
>Mature Secondary Structure
MKIVAVTACPTGIAHTYMAADALTKAAPKYNVQIKVETQGAMGIENPLTPYDLSHADKVL
CEEEEEEECCCCHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCEEE
IVSDIDIEQPARFEGMKVVKLSIEEVLLNVDKVFLVHCR
EEECCCCCCCCCCCCEEEEEEEHHHHHHCCCEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]