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The map label for this gene is frwBC [H]

Identifier: 227081950

GI number: 227081950

Start: 1897940

End: 1899343

Strand: Direct

Name: frwBC [H]

Synonym: VCM66_1744

Alternate gene names: 227081950

Gene position: 1897940-1899343 (Clockwise)

Preceding gene: 227081949

Following gene: 227081952

Centisome position: 65.62

GC content: 49.79

Gene sequence:

>1404_bases
ATGAGTACCCTAACCGCTCAAGCTACCAATAATAGTAGCAATTTCAAAAAACTCCTCAGTACCATGAAAGGCCACCTTTT
ATTTGGCACCTCACATATGTTGCCATTTATTGTTGCTGGCGGCGTATTATTGGCACTTGCGGTAATGGCATCCGGCAAAG
GTGCCGTTCCTGCCGATGGCTTATTAGCGGATATTTCCAATATCGGTATTAAAGGTCTGGTGCTGTTCCCGATTATTTTG
GGTGGTTTCATCGGTTATTCCATTGCAGACAAACCAGCTCTTGCGCCAGCGATGATCTCTTCTGGCATCATGGCGGATAT
GGGCGGCGGTTTCCTTGGTTGTATCGTAGCGGGCTTCATCGCCGGTGGTGTGGTATTCCAACTTAAGAAGATCCCTCTTT
CGGCTAACATGACTGCGTTGGGCGCTTACTTCATCTACCCACTTATCGGTACACTTATCTCCGCAGGCATCGTTTTATGG
GGTATCGGTGAGCCAATCAAACTGTTCATGGCGTCTATGAACGAATTCCTAGCTTCTATGGCTGGCGCTTCTAAAGTGGT
GTTAGGTGCGATTCTTGGTGGTATGACGGCATTCGATATGGGCGGTCCAATCAACAAGGTCGCAACGCTATTCGCTCAAA
CTCAAGTGGATACTCAACCATGGCTAATGGGTGGTGTGGGCATCGCAATCTGTACGCCTCCTCTCGGCATGGCGCTAGCC
ACGTTCCTGTTCAAGAAAAAATTCACCAAACAAGAACAAGAAGCAGGTAAAGCAGCAGCAATCATGGGTTCTATCGGTAT
CTCTGAAGGCGCTATCCCATTCGCAGCAAATGACCCAATGCGCGTGCTTCCTTCCATCGTTGCTGGTGGTATCGTTGGCT
GTGTGTTTGGCTTCCTGACTAACGTTCTGCTTCATGCTCCTTGGGGCGGTCTCATCACAGCACCGGTATCAAGCAACATT
CCAATGTACGTTGTAGGTATCGCACTAGGCTCACTCACCACTGCACTCATCGTTGGCTTCTGGAAACCAGTCGCGGAAGA
AGAAGTGGAAGACGAAATCGCAGAAGCTGCACCAACGCAAGCTCAAGCGGCTCCAGCAGCCGGCGAAGGCGAGTACGATA
TCGTTGCTGTCACCTGTTGCCCATCTGGTGTAGCACACACTTTCATGGCAGCCAAAGCACTTGAGAAAGCAGGCGCAGCC
GCTGGCAGCAAAATCAAAGTCGAAACGCAAGGTCAAAATGGCATTAAAAACCGCATCACTGACCTAGATGTCGCAAACGC
GAAGCTAGTTATCCTCGCTCACGATATCCAAGTGAAAGATGCTCACCGCTTTGCCAACGCGAAAGTCATCGAATGCTCGA
CCAAGGAGGCGATGAAAAAAGCCGCCGAACTTATCCAAGCTTAA

Upstream 100 bases:

>100_bases
TATTCGGAAAATTTAGCGAAGAATTTATTGAAGATATTCATAACGCAAAAAATGAACATCAGATCCTTAATATTTCAATT
CAATCATAAGGCGTGAAATT

Downstream 100 bases:

>100_bases
ACCTTAAGCTCCTCCCCCAGAGCCCTTTCCCCCAAAGGGCTCATTTTCTATCTCAAGTGTAGTGATTCAGAATGTTATCG
CTTTATCTGCACCAATGCCG

Product: PTS system, fructose-specific IIBC component

Products: protein histidine; sugar phosphate

Alternate protein names: PTS system fructose-like EIIC component 2 [H]

Number of amino acids: Translated: 467; Mature: 466

Protein sequence:

>467_residues
MSTLTAQATNNSSNFKKLLSTMKGHLLFGTSHMLPFIVAGGVLLALAVMASGKGAVPADGLLADISNIGIKGLVLFPIIL
GGFIGYSIADKPALAPAMISSGIMADMGGGFLGCIVAGFIAGGVVFQLKKIPLSANMTALGAYFIYPLIGTLISAGIVLW
GIGEPIKLFMASMNEFLASMAGASKVVLGAILGGMTAFDMGGPINKVATLFAQTQVDTQPWLMGGVGIAICTPPLGMALA
TFLFKKKFTKQEQEAGKAAAIMGSIGISEGAIPFAANDPMRVLPSIVAGGIVGCVFGFLTNVLLHAPWGGLITAPVSSNI
PMYVVGIALGSLTTALIVGFWKPVAEEEVEDEIAEAAPTQAQAAPAAGEGEYDIVAVTCCPSGVAHTFMAAKALEKAGAA
AGSKIKVETQGQNGIKNRITDLDVANAKLVILAHDIQVKDAHRFANAKVIECSTKEAMKKAAELIQA

Sequences:

>Translated_467_residues
MSTLTAQATNNSSNFKKLLSTMKGHLLFGTSHMLPFIVAGGVLLALAVMASGKGAVPADGLLADISNIGIKGLVLFPIIL
GGFIGYSIADKPALAPAMISSGIMADMGGGFLGCIVAGFIAGGVVFQLKKIPLSANMTALGAYFIYPLIGTLISAGIVLW
GIGEPIKLFMASMNEFLASMAGASKVVLGAILGGMTAFDMGGPINKVATLFAQTQVDTQPWLMGGVGIAICTPPLGMALA
TFLFKKKFTKQEQEAGKAAAIMGSIGISEGAIPFAANDPMRVLPSIVAGGIVGCVFGFLTNVLLHAPWGGLITAPVSSNI
PMYVVGIALGSLTTALIVGFWKPVAEEEVEDEIAEAAPTQAQAAPAAGEGEYDIVAVTCCPSGVAHTFMAAKALEKAGAA
AGSKIKVETQGQNGIKNRITDLDVANAKLVILAHDIQVKDAHRFANAKVIECSTKEAMKKAAELIQA
>Mature_466_residues
STLTAQATNNSSNFKKLLSTMKGHLLFGTSHMLPFIVAGGVLLALAVMASGKGAVPADGLLADISNIGIKGLVLFPIILG
GFIGYSIADKPALAPAMISSGIMADMGGGFLGCIVAGFIAGGVVFQLKKIPLSANMTALGAYFIYPLIGTLISAGIVLWG
IGEPIKLFMASMNEFLASMAGASKVVLGAILGGMTAFDMGGPINKVATLFAQTQVDTQPWLMGGVGIAICTPPLGMALAT
FLFKKKFTKQEQEAGKAAAIMGSIGISEGAIPFAANDPMRVLPSIVAGGIVGCVFGFLTNVLLHAPWGGLITAPVSSNIP
MYVVGIALGSLTTALIVGFWKPVAEEEVEDEIAEAAPTQAQAAPAAGEGEYDIVAVTCCPSGVAHTFMAAKALEKAGAAA
GSKIKVETQGQNGIKNRITDLDVANAKLVILAHDIQVKDAHRFANAKVIECSTKEAMKKAAELIQA

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitant with their translocation across the cell membrane [H]

COG id: COG1299

COG function: function code G; Phosphotransferase system, fructose-specific IIC component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIC type-2 domain [H]

Homologues:

Organism=Escherichia coli, GI1790386, Length=308, Percent_Identity=46.4285714285714, Blast_Score=266, Evalue=3e-72,
Organism=Escherichia coli, GI1788492, Length=358, Percent_Identity=39.6648044692737, Blast_Score=198, Evalue=5e-52,
Organism=Escherichia coli, GI1786951, Length=338, Percent_Identity=32.5443786982249, Blast_Score=147, Evalue=1e-36,
Organism=Escherichia coli, GI87082348, Length=356, Percent_Identity=31.1797752808989, Blast_Score=140, Evalue=2e-34,
Organism=Escherichia coli, GI1788729, Length=388, Percent_Identity=27.0618556701031, Blast_Score=98, Evalue=1e-21,
Organism=Escherichia coli, GI1790387, Length=88, Percent_Identity=52.2727272727273, Blast_Score=92, Evalue=6e-20,
Organism=Escherichia coli, GI1788730, Length=93, Percent_Identity=44.0860215053763, Blast_Score=86, Evalue=8e-18,
Organism=Escherichia coli, GI1790390, Length=94, Percent_Identity=38.2978723404255, Blast_Score=75, Evalue=6e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013014
- InterPro:   IPR006327 [H]

Pfam domain/function: NA

EC number: 2.7.1.69

Molecular weight: Translated: 47864; Mature: 47733

Theoretical pI: Translated: 7.46; Mature: 7.46

Prosite motif: PS51099 PTS_EIIB_TYPE_2 ; PS51104 PTS_EIIC_TYPE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTLTAQATNNSSNFKKLLSTMKGHLLFGTSHMLPFIVAGGVLLALAVMASGKGAVPADG
CCCEEECCCCCCHHHHHHHHHHCCCEEECCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCH
LLADISNIGIKGLVLFPIILGGFIGYSIADKPALAPAMISSGIMADMGGGFLGCIVAGFI
HHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHCCCHHHCCCHHHHHHHHHHH
AGGVVFQLKKIPLSANMTALGAYFIYPLIGTLISAGIVLWGIGEPIKLFMASMNEFLASM
HCCHHEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCEEEEECCCHHHHHHHHHHHHHHHH
AGASKVVLGAILGGMTAFDMGGPINKVATLFAQTQVDTQPWLMGGVGIAICTPPLGMALA
CCCHHHHHHHHHHCHHHHCCCCCHHHHHHHHHHHCCCCCCEEECCCEEEEECCCHHHHHH
TFLFKKKFTKQEQEAGKAAAIMGSIGISEGAIPFAANDPMRVLPSIVAGGIVGCVFGFLT
HHHHHHHHHHHHHHCCCHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
NVLLHAPWGGLITAPVSSNIPMYVVGIALGSLTTALIVGFWKPVAEEEVEDEIAEAAPTQ
HHHHHCCCCCEEECCCCCCCCEEEHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCCC
AQAAPAAGEGEYDIVAVTCCPSGVAHTFMAAKALEKAGAAAGSKIKVETQGQNGIKNRIT
HHCCCCCCCCCEEEEEEEECCCHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHCC
DLDVANAKLVILAHDIQVKDAHRFANAKVIECSTKEAMKKAAELIQA
CCCCCCEEEEEEEECCEEHHHHHHCCCEEEECCHHHHHHHHHHHHCC
>Mature Secondary Structure 
STLTAQATNNSSNFKKLLSTMKGHLLFGTSHMLPFIVAGGVLLALAVMASGKGAVPADG
CCEEECCCCCCHHHHHHHHHHCCCEEECCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCH
LLADISNIGIKGLVLFPIILGGFIGYSIADKPALAPAMISSGIMADMGGGFLGCIVAGFI
HHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHCCCHHHCCCHHHHHHHHHHH
AGGVVFQLKKIPLSANMTALGAYFIYPLIGTLISAGIVLWGIGEPIKLFMASMNEFLASM
HCCHHEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCEEEEECCCHHHHHHHHHHHHHHHH
AGASKVVLGAILGGMTAFDMGGPINKVATLFAQTQVDTQPWLMGGVGIAICTPPLGMALA
CCCHHHHHHHHHHCHHHHCCCCCHHHHHHHHHHHCCCCCCEEECCCEEEEECCCHHHHHH
TFLFKKKFTKQEQEAGKAAAIMGSIGISEGAIPFAANDPMRVLPSIVAGGIVGCVFGFLT
HHHHHHHHHHHHHHCCCHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
NVLLHAPWGGLITAPVSSNIPMYVVGIALGSLTTALIVGFWKPVAEEEVEDEIAEAAPTQ
HHHHHCCCCCEEECCCCCCCCEEEHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCCC
AQAAPAAGEGEYDIVAVTCCPSGVAHTFMAAKALEKAGAAAGSKIKVETQGQNGIKNRIT
HHCCCCCCCCCEEEEEEEECCCHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHCC
DLDVANAKLVILAHDIQVKDAHRFANAKVIECSTKEAMKKAAELIQA
CCCCCCEEEEEEEECCEEHHHHHHCCCEEEECCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: protein N(pi)-phosphohistidine; sugar

Specific reaction: protein N(pi)-phosphohistidine + sugar = protein histidine + sugar phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8265357; 9278503; 7773398 [H]