Click here to switch to the map view.

The map label for this gene is sapA [H]

Identifier: 227081831

GI number: 227081831

Start: 1760949

End: 1762571

Strand: Direct

Name: sapA [H]

Synonym: VCM66_1620

Alternate gene names: 227081831

Gene position: 1760949-1762571 (Clockwise)

Preceding gene: 227081830

Following gene: 227081832

Centisome position: 60.88

GC content: 49.17

Gene sequence:

>1623_bases
ATGAATGCATTATTACGATTTACGCTAGGGCTGTGCAGCATGACTCTGCTGGCAGGCTGCGGAGAAAACATCGACCACAG
CAAGATCCGCAGTAGCGGCTTTGTCTACTGTGGTGAAAGTGCGCCGACCACCTTTAATCCGCAGTTGGCGGATAACGATA
TCACCTCTGATGCACTCGGTCCGCAGATCTACAACACCTTGCTGACAATAGACCCCGAAACACACTTACCCGTTGCCAGT
TTAGCCAGTAGCTGGACCGTCAATAGCACCGGACTTGAGTATGTGTTTAAGCTGCGTCCCGATGTCGCGTTTCAAACCAC
TCCCTGGTTTAGCCCGACACGTCCCCTCAATGCCGATGACGTCGTGTTCAGTTTTCGACGCATTATTGACCCGACCAATC
CCTACCACAAAGTAGGCCAAACTCAATATCCTTGGTTTAAAGGCATTGATTTTCAGAATCTATTGGTTGATGTCAGCGCG
CTCGATGACTTAACCGTAAAGTTTGTCCTTAGCCGGCCTGATAACAGTTTTTTATCCAACATCGCCACTAGCCACGCGGT
GATTCTGTCGTTGGAATACGCCAATCAGCTCATCATTGATGATGAAAAAGAGAAGCTGGATAACCTTCCTCTGGGTACTG
GGCCTTTTTACCTTGCCGAATACCATCCGCGCGATTTAATTCGCTTGAAACGTCATCCCCAATATTGGGAAGGTGCGGCA
AAAGTCGAGCAAGTGGTGTTTGATATTTCACAACGTGGGACTGGCATGCTGGCCAAATTGCTGCGCAATGAATGTGATGT
GCTCAATGCACCGATTTCCAGCCAACTGCCTGCGATTGAAAAGAACCCAGATATTGTGCTGCAAACCACACCTGCGATGA
ATGTGGCCTTTATCGCGGTGAACACTCAACATCCTGCGTTAAATGACAATCGCGTGCGTAAAGCACTCAATTTCGCGATT
AATCGACAAAATATCTTGGATTCTGTCTATTACGGTACCGGAAGCATCGCTTTTACCATCTTGCCCCCCACCTCTTGGGC
CTATCAGCAAGATACGGCGCAGATTCGCTACGATCGTAACTACGCGCAAGCTTTGCTGCGTGAAGCTGGATTTGCAACGG
GTCTGGAGCTGACCATGTCAGTACCTGTAGAGCCGAAAGCGTACAATCCTAGCCCGCGCAAAACGGCAGAGCTGATCCAA
GCGAATCTCGCCGACATTGGGGTGACATTACGCCTCATCAGTGAAGATCGCTCAGAGCGCCAAGAGCTGAGTATACGCAA
CAATATTGACCTGTACTTAAGTGGCTGGACGGGAGATACGGGTGACCCAGATAACTTCTTACGCCCCTTACTCTCTTGTG
ATTCCAACCGCGCAGGTCTCAACGTTTCCATGTGGTGCGACAGTGATTTTGATTTTCTGTTGGACTTGGCGCTAGAAGCC
AATAAACCGCGCTATCGCCTCAATCTGTATCATCAGGCGCAAAATATCCTCAATCAGGAGTTTCCGGTGATTCCTCTCGC
CCACGGCATACAGTTTACCGCTTATAGCAAATCATTAACGGGCATTCGCATTAGTCCATTTAATGTCCAACCGTTTAATA
CTGTCGAGAGGGTGGCCGAGTAA

Upstream 100 bases:

>100_bases
CGCCTTTTCACTTGGTTTCGTTCGAAAAACAATGGTAAATTAGCGGGATTTTGAGGCTTGCCAGACTCTGGCCAACGCCT
CGTGACTGACTCAGATCACT

Downstream 100 bases:

>100_bases
TGTTCGTCTATACGGTCCGTAAATTTAACTTATTTATCATCACCTTGCTTATCTTGACCATGGTCGGCTACAGCTTAGCC
AGATTTGACCCGCACTCGCC

Product: peptide ABC transporter, periplasmic peptide-binding protein

Products: ADP; phosphate; peptides [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 540; Mature: 540

Protein sequence:

>540_residues
MNALLRFTLGLCSMTLLAGCGENIDHSKIRSSGFVYCGESAPTTFNPQLADNDITSDALGPQIYNTLLTIDPETHLPVAS
LASSWTVNSTGLEYVFKLRPDVAFQTTPWFSPTRPLNADDVVFSFRRIIDPTNPYHKVGQTQYPWFKGIDFQNLLVDVSA
LDDLTVKFVLSRPDNSFLSNIATSHAVILSLEYANQLIIDDEKEKLDNLPLGTGPFYLAEYHPRDLIRLKRHPQYWEGAA
KVEQVVFDISQRGTGMLAKLLRNECDVLNAPISSQLPAIEKNPDIVLQTTPAMNVAFIAVNTQHPALNDNRVRKALNFAI
NRQNILDSVYYGTGSIAFTILPPTSWAYQQDTAQIRYDRNYAQALLREAGFATGLELTMSVPVEPKAYNPSPRKTAELIQ
ANLADIGVTLRLISEDRSERQELSIRNNIDLYLSGWTGDTGDPDNFLRPLLSCDSNRAGLNVSMWCDSDFDFLLDLALEA
NKPRYRLNLYHQAQNILNQEFPVIPLAHGIQFTAYSKSLTGIRISPFNVQPFNTVERVAE

Sequences:

>Translated_540_residues
MNALLRFTLGLCSMTLLAGCGENIDHSKIRSSGFVYCGESAPTTFNPQLADNDITSDALGPQIYNTLLTIDPETHLPVAS
LASSWTVNSTGLEYVFKLRPDVAFQTTPWFSPTRPLNADDVVFSFRRIIDPTNPYHKVGQTQYPWFKGIDFQNLLVDVSA
LDDLTVKFVLSRPDNSFLSNIATSHAVILSLEYANQLIIDDEKEKLDNLPLGTGPFYLAEYHPRDLIRLKRHPQYWEGAA
KVEQVVFDISQRGTGMLAKLLRNECDVLNAPISSQLPAIEKNPDIVLQTTPAMNVAFIAVNTQHPALNDNRVRKALNFAI
NRQNILDSVYYGTGSIAFTILPPTSWAYQQDTAQIRYDRNYAQALLREAGFATGLELTMSVPVEPKAYNPSPRKTAELIQ
ANLADIGVTLRLISEDRSERQELSIRNNIDLYLSGWTGDTGDPDNFLRPLLSCDSNRAGLNVSMWCDSDFDFLLDLALEA
NKPRYRLNLYHQAQNILNQEFPVIPLAHGIQFTAYSKSLTGIRISPFNVQPFNTVERVAE
>Mature_540_residues
MNALLRFTLGLCSMTLLAGCGENIDHSKIRSSGFVYCGESAPTTFNPQLADNDITSDALGPQIYNTLLTIDPETHLPVAS
LASSWTVNSTGLEYVFKLRPDVAFQTTPWFSPTRPLNADDVVFSFRRIIDPTNPYHKVGQTQYPWFKGIDFQNLLVDVSA
LDDLTVKFVLSRPDNSFLSNIATSHAVILSLEYANQLIIDDEKEKLDNLPLGTGPFYLAEYHPRDLIRLKRHPQYWEGAA
KVEQVVFDISQRGTGMLAKLLRNECDVLNAPISSQLPAIEKNPDIVLQTTPAMNVAFIAVNTQHPALNDNRVRKALNFAI
NRQNILDSVYYGTGSIAFTILPPTSWAYQQDTAQIRYDRNYAQALLREAGFATGLELTMSVPVEPKAYNPSPRKTAELIQ
ANLADIGVTLRLISEDRSERQELSIRNNIDLYLSGWTGDTGDPDNFLRPLLSCDSNRAGLNVSMWCDSDFDFLLDLALEA
NKPRYRLNLYHQAQNILNQEFPVIPLAHGIQFTAYSKSLTGIRISPFNVQPFNTVERVAE

Specific function: Involved in a peptide intake transport system that plays a role in the resistance to antimicrobial peptides [H]

COG id: COG4166

COG function: function code E; ABC-type oligopeptide transport system, periplasmic component

Gene ontology:

Cell location: Periplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1787551, Length=528, Percent_Identity=43.3712121212121, Blast_Score=435, Evalue=1e-123,
Organism=Escherichia coli, GI1789966, Length=535, Percent_Identity=36.4485981308411, Blast_Score=372, Evalue=1e-104,
Organism=Escherichia coli, GI1787052, Length=526, Percent_Identity=28.1368821292776, Blast_Score=179, Evalue=5e-46,
Organism=Escherichia coli, GI1787762, Length=456, Percent_Identity=24.1228070175439, Blast_Score=121, Evalue=1e-28,
Organism=Escherichia coli, GI1789397, Length=510, Percent_Identity=27.0588235294118, Blast_Score=109, Evalue=4e-25,
Organism=Escherichia coli, GI1787495, Length=473, Percent_Identity=25.5813953488372, Blast_Score=107, Evalue=3e-24,
Organism=Escherichia coli, GI1789887, Length=568, Percent_Identity=23.2394366197183, Blast_Score=105, Evalue=1e-23,
Organism=Escherichia coli, GI87081878, Length=456, Percent_Identity=24.780701754386, Blast_Score=91, Evalue=2e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 60453; Mature: 60453

Theoretical pI: Translated: 5.00; Mature: 5.00

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNALLRFTLGLCSMTLLAGCGENIDHSKIRSSGFVYCGESAPTTFNPQLADNDITSDALG
CCHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCCEEECCCCCCCCCCCCCCCCCCCHHCC
PQIYNTLLTIDPETHLPVASLASSWTVNSTGLEYVFKLRPDVAFQTTPWFSPTRPLNADD
HHHHHEEEEECCCCCCCHHHHHCCCEECCCCCEEEEEECCCCEEEECCCCCCCCCCCHHH
VVFSFRRIIDPTNPYHKVGQTQYPWFKGIDFQNLLVDVSALDDLTVKFVLSRPDNSFLSN
HHHHHHHHCCCCCCHHHCCCCCCCCCCCCCHHHHHEEHHHHCCEEEEEEEECCCHHHHHH
IATSHAVILSLEYANQLIIDDEKEKLDNLPLGTGPFYLAEYHPRDLIRLKRHPQYWEGAA
HHCCCEEEEEEECCCEEEECCCHHHHCCCCCCCCCEEEEECCCHHHHHHHCCCCHHHHHH
KVEQVVFDISQRGTGMLAKLLRNECDVLNAPISSQLPAIEKNPDIVLQTTPAMNVAFIAV
HHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCHHHCCCCCCCCCCEEEEECCCCEEEEEEE
NTQHPALNDNRVRKALNFAINRQNILDSVYYGTGSIAFTILPPTSWAYQQDTAQIRYDRN
ECCCCCCCHHHHHHHHHHHHCHHHHHHHHEECCCCEEEEEECCCCCCCCCCCEEEEECCH
YAQALLREAGFATGLELTMSVPVEPKAYNPSPRKTAELIQANLADIGVTLRLISEDRSER
HHHHHHHHCCCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHCEEEEEECCCHHHH
QELSIRNNIDLYLSGWTGDTGDPDNFLRPLLSCDSNRAGLNVSMWCDSDFDFLLDLALEA
HHHHHCCCEEEEEECCCCCCCCHHHHHHHHHHCCCCCCCCEEEEEECCCHHHHHHHHHCC
NKPRYRLNLYHQAQNILNQEFPVIPLAHGIQFTAYSKSLTGIRISPFNVQPFNTVERVAE
CCCEEEEEHHHHHHHHHCCCCCEEEECCCEEEEEECCCCCEEEECCCCCCCCHHHHHHCC
>Mature Secondary Structure
MNALLRFTLGLCSMTLLAGCGENIDHSKIRSSGFVYCGESAPTTFNPQLADNDITSDALG
CCHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCCEEECCCCCCCCCCCCCCCCCCCHHCC
PQIYNTLLTIDPETHLPVASLASSWTVNSTGLEYVFKLRPDVAFQTTPWFSPTRPLNADD
HHHHHEEEEECCCCCCCHHHHHCCCEECCCCCEEEEEECCCCEEEECCCCCCCCCCCHHH
VVFSFRRIIDPTNPYHKVGQTQYPWFKGIDFQNLLVDVSALDDLTVKFVLSRPDNSFLSN
HHHHHHHHCCCCCCHHHCCCCCCCCCCCCCHHHHHEEHHHHCCEEEEEEEECCCHHHHHH
IATSHAVILSLEYANQLIIDDEKEKLDNLPLGTGPFYLAEYHPRDLIRLKRHPQYWEGAA
HHCCCEEEEEEECCCEEEECCCHHHHCCCCCCCCCEEEEECCCHHHHHHHCCCCHHHHHH
KVEQVVFDISQRGTGMLAKLLRNECDVLNAPISSQLPAIEKNPDIVLQTTPAMNVAFIAV
HHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCHHHCCCCCCCCCCEEEEECCCCEEEEEEE
NTQHPALNDNRVRKALNFAINRQNILDSVYYGTGSIAFTILPPTSWAYQQDTAQIRYDRN
ECCCCCCCHHHHHHHHHHHHCHHHHHHHHEECCCCEEEEEECCCCCCCCCCCEEEEECCH
YAQALLREAGFATGLELTMSVPVEPKAYNPSPRKTAELIQANLADIGVTLRLISEDRSER
HHHHHHHHCCCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHCEEEEEECCCHHHH
QELSIRNNIDLYLSGWTGDTGDPDNFLRPLLSCDSNRAGLNVSMWCDSDFDFLLDLALEA
HHHHHCCCEEEEEECCCCCCCCHHHHHHHHHHCCCCCCCCEEEEEECCCHHHHHHHHHCC
NKPRYRLNLYHQAQNILNQEFPVIPLAHGIQFTAYSKSLTGIRISPFNVQPFNTVERVAE
CCCEEEEEHHHHHHHHHCCCCCEEEECCCEEEEEECCCCCEEEECCCCCCCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; peptides [Periplasm]; H2O [C]

Specific reaction: ATP + peptides [Periplasm] + H2O = ADP + phosphate + peptides [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8223423; 11677609 [H]