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The map label for this gene is pspF [H]

Identifier: 227081830

GI number: 227081830

Start: 1759819

End: 1760835

Strand: Direct

Name: pspF [H]

Synonym: VCM66_1619

Alternate gene names: 227081830

Gene position: 1759819-1760835 (Clockwise)

Preceding gene: 227081822

Following gene: 227081831

Centisome position: 60.84

GC content: 50.44

Gene sequence:

>1017_bases
ATGCAACAGAGCTTGCTCGGAGAGTCACCTGCGTTTTTAGCGGTGTTAGACAAAGTCTCACGTTTAGCGCCGATCGATCG
TCCTGTGCTGGTCATTGGCGAGCGCGGAACGGGTAAAGAGTTGATCGCCCAGCGCTTGCACTATTTATCAAAACGTTGGG
AACAACCGCTGGTGTCGCTAAACTGCTCGACGCTCAGTGAAGGACTGATCGATTCAGAGCTGTTTGGCCATGAGTCAGGC
TCGTTTACTGGAGCCAAAGGACGCCATCAAGGACGTTTTGAACGTGCCGAAGGCGGTACGCTGTTTTTAGATGAATTGGC
CACTGCACCACTCTCAGTGCAAGAAAAACTGCTACGCGTGATCGAATACGGCCAATATGAACGTGTCGGTGGTAACCAAG
TATTAAACGCGAATGTGCGCTTGGTATGTGCAACCAACGCGAATTTACCGCAAATGGCTCAGCAAGGCACATTTCGCGCA
GACTTACTGGACCGACTGGCGTTTGACGTGATTCATTTGCCCGCACTTCGCCATCGGCCAGAAGATATTGCGCTGCTTGC
CGAACATTTTGCTATTCGCATGTGCCGTGAACTACAACTGCCACTCTTTGTTGGCTTTAGCCCCAATGCATTACACCAAC
TGCAAGCCTACTCTTGGCCGGGCAATGTGCGTGAGCTGAAAAACGTGGTGGAGCGCGCGGTGTATCAACATGGGTTGAAT
AGCCAACCAATTGATCAGTTAGTCTTTAACCCGTTCCAATCCAGTGACATTGTCACCGATGAGTCTGAGCAGGTTGACGT
TCGCCATACCACTCGTACCGACTTCCATTTGCCTCTTGACTACAAGGCATGGCAGCAACAGTGTGATATTGAGTTGTTAC
AAGCGGCACTCACGCAAGCGAAATTTAATCAAAAGCACGCCGCGCAGTTGCTGGGACTCAGCTATCATCAATTTCGAGGC
ATGATGAGAAAGTACCCACAAATGAACAGTGAAAACGCCTCGCCTCACGTTGAGTGA

Upstream 100 bases:

>100_bases
ATTTTTCAAACAATTACAAATATACCGACTTTTTCACTTAGCATGCTATGATGAAATTCGCCAACAGTTGGTCAATTTCA
CCACTTTGAGGAAATAGAGA

Downstream 100 bases:

>100_bases
TATGCGGTAAATGCGCCTTTTCACTTGGTTTCGTTCGAAAAACAATGGTAAATTAGCGGGATTTTGAGGCTTGCCAGACT
CTGGCCAACGCCTCGTGACT

Product: psp operon transcriptional activator

Products: NA

Alternate protein names: Phage shock protein F [H]

Number of amino acids: Translated: 338; Mature: 338

Protein sequence:

>338_residues
MQQSLLGESPAFLAVLDKVSRLAPIDRPVLVIGERGTGKELIAQRLHYLSKRWEQPLVSLNCSTLSEGLIDSELFGHESG
SFTGAKGRHQGRFERAEGGTLFLDELATAPLSVQEKLLRVIEYGQYERVGGNQVLNANVRLVCATNANLPQMAQQGTFRA
DLLDRLAFDVIHLPALRHRPEDIALLAEHFAIRMCRELQLPLFVGFSPNALHQLQAYSWPGNVRELKNVVERAVYQHGLN
SQPIDQLVFNPFQSSDIVTDESEQVDVRHTTRTDFHLPLDYKAWQQQCDIELLQAALTQAKFNQKHAAQLLGLSYHQFRG
MMRKYPQMNSENASPHVE

Sequences:

>Translated_338_residues
MQQSLLGESPAFLAVLDKVSRLAPIDRPVLVIGERGTGKELIAQRLHYLSKRWEQPLVSLNCSTLSEGLIDSELFGHESG
SFTGAKGRHQGRFERAEGGTLFLDELATAPLSVQEKLLRVIEYGQYERVGGNQVLNANVRLVCATNANLPQMAQQGTFRA
DLLDRLAFDVIHLPALRHRPEDIALLAEHFAIRMCRELQLPLFVGFSPNALHQLQAYSWPGNVRELKNVVERAVYQHGLN
SQPIDQLVFNPFQSSDIVTDESEQVDVRHTTRTDFHLPLDYKAWQQQCDIELLQAALTQAKFNQKHAAQLLGLSYHQFRG
MMRKYPQMNSENASPHVE
>Mature_338_residues
MQQSLLGESPAFLAVLDKVSRLAPIDRPVLVIGERGTGKELIAQRLHYLSKRWEQPLVSLNCSTLSEGLIDSELFGHESG
SFTGAKGRHQGRFERAEGGTLFLDELATAPLSVQEKLLRVIEYGQYERVGGNQVLNANVRLVCATNANLPQMAQQGTFRA
DLLDRLAFDVIHLPALRHRPEDIALLAEHFAIRMCRELQLPLFVGFSPNALHQLQAYSWPGNVRELKNVVERAVYQHGLN
SQPIDQLVFNPFQSSDIVTDESEQVDVRHTTRTDFHLPLDYKAWQQQCDIELLQAALTQAKFNQKHAAQLLGLSYHQFRG
MMRKYPQMNSENASPHVE

Specific function: Transcriptional activator for the phage shock protein (psp) operon (pspABCDE) and pspG gene [H]

COG id: COG1221

COG function: function code KT; Transcriptional regulators containing an AAA-type ATPase domain and a DNA-binding domain

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI87081872, Length=324, Percent_Identity=62.962962962963, Blast_Score=427, Evalue=1e-121,
Organism=Escherichia coli, GI1788550, Length=326, Percent_Identity=41.1042944785276, Blast_Score=231, Evalue=5e-62,
Organism=Escherichia coli, GI87082117, Length=230, Percent_Identity=49.1304347826087, Blast_Score=224, Evalue=7e-60,
Organism=Escherichia coli, GI1790437, Length=233, Percent_Identity=48.4978540772532, Blast_Score=220, Evalue=8e-59,
Organism=Escherichia coli, GI1789087, Length=230, Percent_Identity=47.3913043478261, Blast_Score=215, Evalue=3e-57,
Organism=Escherichia coli, GI1790299, Length=335, Percent_Identity=39.1044776119403, Blast_Score=205, Evalue=4e-54,
Organism=Escherichia coli, GI1788905, Length=326, Percent_Identity=36.8098159509202, Blast_Score=204, Evalue=8e-54,
Organism=Escherichia coli, GI87082152, Length=233, Percent_Identity=45.4935622317597, Blast_Score=194, Evalue=8e-51,
Organism=Escherichia coli, GI1789233, Length=332, Percent_Identity=34.9397590361446, Blast_Score=193, Evalue=2e-50,
Organism=Escherichia coli, GI1786524, Length=244, Percent_Identity=43.0327868852459, Blast_Score=175, Evalue=3e-45,
Organism=Escherichia coli, GI1787583, Length=245, Percent_Identity=37.9591836734694, Blast_Score=164, Evalue=8e-42,
Organism=Escherichia coli, GI1789828, Length=249, Percent_Identity=30.9236947791165, Blast_Score=108, Evalue=4e-25,
Organism=Escherichia coli, GI87081858, Length=204, Percent_Identity=30.8823529411765, Blast_Score=98, Evalue=8e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR014317 [H]

Pfam domain/function: PF02954 HTH_8; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 38156; Mature: 38156

Theoretical pI: Translated: 6.70; Mature: 6.70

Prosite motif: PS00676 SIGMA54_INTERACT_2 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQQSLLGESPAFLAVLDKVSRLAPIDRPVLVIGERGTGKELIAQRLHYLSKRWEQPLVSL
CCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHCCCCEEE
NCSTLSEGLIDSELFGHESGSFTGAKGRHQGRFERAEGGTLFLDELATAPLSVQEKLLRV
CHHHHHHCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCEEEHHHHCCCCHHHHHHHHHH
IEYGQYERVGGNQVLNANVRLVCATNANLPQMAQQGTFRADLLDRLAFDVIHLPALRHRP
HHCCCCEECCCCEEEECCEEEEEECCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCC
EDIALLAEHFAIRMCRELQLPLFVGFSPNALHQLQAYSWPGNVRELKNVVERAVYQHGLN
HHHHHHHHHHHHHHHHHCCCCEEECCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCC
SQPIDQLVFNPFQSSDIVTDESEQVDVRHTTRTDFHLPLDYKAWQQQCDIELLQAALTQA
CCCHHHHHCCCCCCCCCCCCCCCCEEEEECCCCCEECCCCHHHHHHHHHHHHHHHHHHHH
KFNQKHAAQLLGLSYHQFRGMMRKYPQMNSENASPHVE
HHCHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure
MQQSLLGESPAFLAVLDKVSRLAPIDRPVLVIGERGTGKELIAQRLHYLSKRWEQPLVSL
CCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHCCCCEEE
NCSTLSEGLIDSELFGHESGSFTGAKGRHQGRFERAEGGTLFLDELATAPLSVQEKLLRV
CHHHHHHCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCEEEHHHHCCCCHHHHHHHHHH
IEYGQYERVGGNQVLNANVRLVCATNANLPQMAQQGTFRADLLDRLAFDVIHLPALRHRP
HHCCCCEECCCCEEEECCEEEEEECCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCC
EDIALLAEHFAIRMCRELQLPLFVGFSPNALHQLQAYSWPGNVRELKNVVERAVYQHGLN
HHHHHHHHHHHHHHHHHCCCCEEECCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCC
SQPIDQLVFNPFQSSDIVTDESEQVDVRHTTRTDFHLPLDYKAWQQQCDIELLQAALTQA
CCCHHHHHCCCCCCCCCCCCCCCCEEEEECCCCCEECCCCHHHHHHHHHHHHHHHHHHHH
KFNQKHAAQLLGLSYHQFRGMMRKYPQMNSENASPHVE
HHCHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8606168; 9097039; 9278503; 1712397; 7984428; 7920643 [H]