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The map label for this gene is oprF [H]

Identifier: 227081775

GI number: 227081775

Start: 1690231

End: 1690869

Strand: Direct

Name: oprF [H]

Synonym: VCM66_1562

Alternate gene names: 227081775

Gene position: 1690231-1690869 (Clockwise)

Preceding gene: 227081774

Following gene: 227081784

Centisome position: 58.43

GC content: 46.64

Gene sequence:

>639_bases
ATGTCAATCAAATCATGGGCTCTGCCACTATTTCTTTGCACTGTAACCACGTCGGCATTGGCAAATCAAGGTCAATATAC
AAAGCACGAAGGTGATGAGTACGACTATCGTCCAACTCCACTCGCGAATCAGATTGCGGATCTGCAAGATGACGATAGAG
ACGGTGTTATCAATGCTCGCGATCTTTGCCCTGATACACCTAAGGGCTCTGAAATCGATAATGATGGCTGTGGCACCTTT
GTCAAATCATCACAGATGCAGCAATTACATATTCTGTTTGCTAATGACTCGAGCTCGATTGAGCCTGCGTTTTTAACGCA
AATCCGTCAAATGGCTGAGTTTTTGCAAACCTATCCTTCAACATCTATTGAGTTACAAGGCTACGCGAGCAAAGTAGGCA
GCGCGGAACATAACCTTGCCCTCTCTAAGCAGCGTTCAGAAGCGGTTCGTGACCAATTACTGCGTTATGGCGTAGCGCCA
GAGCGCGTGAACATTGTCGGATACGGGGATAGCGTTTTGGAAGTAGAAGGTACCGATGATGTGAGCCACGCTCGTAACCG
TCGTGTTACTGCAACAGTTGTTGGCTACAAAGGTGAAGTGGTCAAAGAGTGGACGATTTTTACGACACTTCCTATGTAA

Upstream 100 bases:

>100_bases
CAATATGCAAAATATCGTGTCATGAATGCATCTGGTAACTTGTTAGATGCCTTAAGAGTCGATATCCCGCAGGAATGGAC
AGCTAAGGTGGAGTACTAAT

Downstream 100 bases:

>100_bases
TCACTATCTTCACACATCAAAAAGCCGTGATTACTCACGGCTTTTTTGTTGTTTTTTATTGGCTATCGTCATCAAGGGTG
AGTAATCCTAATCGCTTAAC

Product: putative outer membrane protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 212; Mature: 211

Protein sequence:

>212_residues
MSIKSWALPLFLCTVTTSALANQGQYTKHEGDEYDYRPTPLANQIADLQDDDRDGVINARDLCPDTPKGSEIDNDGCGTF
VKSSQMQQLHILFANDSSSIEPAFLTQIRQMAEFLQTYPSTSIELQGYASKVGSAEHNLALSKQRSEAVRDQLLRYGVAP
ERVNIVGYGDSVLEVEGTDDVSHARNRRVTATVVGYKGEVVKEWTIFTTLPM

Sequences:

>Translated_212_residues
MSIKSWALPLFLCTVTTSALANQGQYTKHEGDEYDYRPTPLANQIADLQDDDRDGVINARDLCPDTPKGSEIDNDGCGTF
VKSSQMQQLHILFANDSSSIEPAFLTQIRQMAEFLQTYPSTSIELQGYASKVGSAEHNLALSKQRSEAVRDQLLRYGVAP
ERVNIVGYGDSVLEVEGTDDVSHARNRRVTATVVGYKGEVVKEWTIFTTLPM
>Mature_211_residues
SIKSWALPLFLCTVTTSALANQGQYTKHEGDEYDYRPTPLANQIADLQDDDRDGVINARDLCPDTPKGSEIDNDGCGTFV
KSSQMQQLHILFANDSSSIEPAFLTQIRQMAEFLQTYPSTSIELQGYASKVGSAEHNLALSKQRSEAVRDQLLRYGVAPE
RVNIVGYGDSVLEVEGTDDVSHARNRRVTATVVGYKGEVVKEWTIFTTLPM

Specific function: Has porin activity, forming small water-filled channels. Also has a structural role in determining cell shape and ability to grow in low-osmolarity medium [H]

COG id: COG2885

COG function: function code M; Outer membrane protein and related peptidoglycan-associated (lipo)proteins

Gene ontology:

Cell location: Cell outer membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 OmpA-like domain [H]

Homologues:

Organism=Escherichia coli, GI1786962, Length=100, Percent_Identity=31, Blast_Score=62, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011250
- InterPro:   IPR006664
- InterPro:   IPR006690
- InterPro:   IPR006665
- InterPro:   IPR008722 [H]

Pfam domain/function: PF00691 OmpA; PF05736 OprF [H]

EC number: NA

Molecular weight: Translated: 23466; Mature: 23335

Theoretical pI: Translated: 4.58; Mature: 4.58

Prosite motif: PS00018 EF_HAND_1 ; PS51123 OMPA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIKSWALPLFLCTVTTSALANQGQYTKHEGDEYDYRPTPLANQIADLQDDDRDGVINAR
CCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCCCEEHH
DLCPDTPKGSEIDNDGCGTFVKSSQMQQLHILFANDSSSIEPAFLTQIRQMAEFLQTYPS
HCCCCCCCCCCCCCCCCCHHHHCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCC
TSIELQGYASKVGSAEHNLALSKQRSEAVRDQLLRYGVAPERVNIVGYGDSVLEVEGTDD
CCEEEECHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCEEEEECCCC
VSHARNRRVTATVVGYKGEVVKEWTIFTTLPM
HHHHCCCEEEEEEECCCCCEEEEEEEEEECCC
>Mature Secondary Structure 
SIKSWALPLFLCTVTTSALANQGQYTKHEGDEYDYRPTPLANQIADLQDDDRDGVINAR
CCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCCCEEHH
DLCPDTPKGSEIDNDGCGTFVKSSQMQQLHILFANDSSSIEPAFLTQIRQMAEFLQTYPS
HCCCCCCCCCCCCCCCCCHHHHCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCC
TSIELQGYASKVGSAEHNLALSKQRSEAVRDQLLRYGVAPERVNIVGYGDSVLEVEGTDD
CCEEEECHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCEEEEECCCC
VSHARNRRVTATVVGYKGEVVKEWTIFTTLPM
HHHHCCCEEEEEEECCCCCEEEEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2447060; 10984043 [H]