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The map label for this gene is cheB-1

Identifier: 227081572

GI number: 227081572

Start: 1448651

End: 1449727

Strand: Reverse

Name: cheB-1

Synonym: VCM66_1356

Alternate gene names: 227081572

Gene position: 1449727-1448651 (Counterclockwise)

Preceding gene: 227081573

Following gene: 227081571

Centisome position: 50.12

GC content: 48.75

Gene sequence:

>1077_bases
ATGAAGATTTTAGTTGTCGATGATTCTGCGTTAATGCGCACCACCATTAGCGACATATTGCAAAACATTCCGAATGCCGA
GATTAAAACAGCACGTGATGGTATGGATGCCATCGACAAAGTGATGAAATGGCAACCGGATGTGATGACGTTAGACATCA
ATATGCCCAACATGGATGGTCTAACTTGCCTTACGCAAATCATGGTTGAACGTCCCTTGCCGATTGTCATGCTCTCATCC
CTCACCCATGAAGGTGCCATTACAACGCTCGAAGCGCTTTATCTTGGTGCGGTAGACTTTGTCGCCAAACCCGGAGGCAC
TATCTGCGGTCGATTACAAGAAGTCGCACCACTGATCCGTGAAAAAGTTCGCGCGGCAGCGAAAATGAGAGTCAAAGTTC
ACACGCAGCTTTCGGTATCACCAGCAGAAGTAGAGCACAAACATAAGTCACCTGTGGTGGAAAATAATGAAGACCACCAG
CTTGCGATCATGGGGGTTTCGACAGGAGGGCCCGGCACTGTTGAAACGATACTGCGCCATCTGCCCGCCGATTTCTCATT
ACCTATTATTGTTAACCAACACATGCCAGAGAGCTTCACTGCCGCCTTTGCACAAAGGCTTAATCGCCATCTGCAACAAC
CCGTGAAAGAGATTAACCGTGCTTTAGTGCTTGAGGCTGGTACCGTTTATGTTTGCAAGGGTGACCGAGACTGCATTGTC
ACGCTACGTGATGGTAAATTGGCTGCCATGCCCACGCCAAATGATAGCCATTTTTCATGGCATCCCAGCGTCTCCAAACT
GGTTGCCAGCGCCATACGCACTTGTGGAGAAGATAACTTATTGTGCGTCATGCTAACGGGAATGGGCGACGACGGTGCCA
CTGAAATGGCTCAGGTTGCTCAAGGAAACGGTATTGTTTTTGCCCAAGAACCCACAACCTGCGTAGTCCCCAGTATGCCG
GAAGCATTACTTAAACGTGTACCCCATATTCCAACAGGAACTCCAGAGCACTTAGCTTATCTGATGACCGAAGTGGTGAG
ACAGTCCAGTAGGGAGCTTCACTATGGCAATCATTAA

Upstream 100 bases:

>100_bases
GAGTACTGTCCGCCGATCAATTGATCAGTAATACCGAGATGTACAAGTTACACATGGCTGCAAACGATATGGATAGCTAA
GTTTTAAGGCTGGAGGGAAC

Downstream 100 bases:

>100_bases
ACGCAGCGCTGCTACAGACAACCCAAATATTGAGATACTTTCTACTCCCGAGCAGATCGATGTGCTGAGCGCAGAAGCGC
TGATGGAAGCTCTACAAGGT

Product: protein-glutamate methylesterase CheB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 358; Mature: 358

Protein sequence:

>358_residues
MKILVVDDSALMRTTISDILQNIPNAEIKTARDGMDAIDKVMKWQPDVMTLDINMPNMDGLTCLTQIMVERPLPIVMLSS
LTHEGAITTLEALYLGAVDFVAKPGGTICGRLQEVAPLIREKVRAAAKMRVKVHTQLSVSPAEVEHKHKSPVVENNEDHQ
LAIMGVSTGGPGTVETILRHLPADFSLPIIVNQHMPESFTAAFAQRLNRHLQQPVKEINRALVLEAGTVYVCKGDRDCIV
TLRDGKLAAMPTPNDSHFSWHPSVSKLVASAIRTCGEDNLLCVMLTGMGDDGATEMAQVAQGNGIVFAQEPTTCVVPSMP
EALLKRVPHIPTGTPEHLAYLMTEVVRQSSRELHYGNH

Sequences:

>Translated_358_residues
MKILVVDDSALMRTTISDILQNIPNAEIKTARDGMDAIDKVMKWQPDVMTLDINMPNMDGLTCLTQIMVERPLPIVMLSS
LTHEGAITTLEALYLGAVDFVAKPGGTICGRLQEVAPLIREKVRAAAKMRVKVHTQLSVSPAEVEHKHKSPVVENNEDHQ
LAIMGVSTGGPGTVETILRHLPADFSLPIIVNQHMPESFTAAFAQRLNRHLQQPVKEINRALVLEAGTVYVCKGDRDCIV
TLRDGKLAAMPTPNDSHFSWHPSVSKLVASAIRTCGEDNLLCVMLTGMGDDGATEMAQVAQGNGIVFAQEPTTCVVPSMP
EALLKRVPHIPTGTPEHLAYLMTEVVRQSSRELHYGNH
>Mature_358_residues
MKILVVDDSALMRTTISDILQNIPNAEIKTARDGMDAIDKVMKWQPDVMTLDINMPNMDGLTCLTQIMVERPLPIVMLSS
LTHEGAITTLEALYLGAVDFVAKPGGTICGRLQEVAPLIREKVRAAAKMRVKVHTQLSVSPAEVEHKHKSPVVENNEDHQ
LAIMGVSTGGPGTVETILRHLPADFSLPIIVNQHMPESFTAAFAQRLNRHLQQPVKEINRALVLEAGTVYVCKGDRDCIV
TLRDGKLAAMPTPNDSHFSWHPSVSKLVASAIRTCGEDNLLCVMLTGMGDDGATEMAQVAQGNGIVFAQEPTTCVVPSMP
EALLKRVPHIPTGTPEHLAYLMTEVVRQSSRELHYGNH

Specific function: Involved in the modulation of the chemotaxis system; catalyzes the demethylation of specific methylglutamate residues introduced into the chemoreceptors (methyl-accepting chemotaxis proteins) by CheR

COG id: COG2201

COG function: function code NT; Chemotaxis response regulator containing a CheY-like receiver domain and a methylesterase domain

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain

Homologues:

Organism=Escherichia coli, GI1788192, Length=323, Percent_Identity=38.6996904024768, Blast_Score=188, Evalue=6e-49,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): CHEB3_VIBCH (Q9KS59)

Other databases:

- EMBL:   AE003852
- PIR:   A82206
- RefSeq:   NP_231044.1
- ProteinModelPortal:   Q9KS59
- SMR:   Q9KS59
- GeneID:   2614033
- GenomeReviews:   AE003852_GR
- KEGG:   vch:VC1401
- TIGR:   VC_1401
- HOGENOM:   HBG705324
- OMA:   PADFPWP
- ProtClustDB:   CLSK794127
- BRENDA:   3.1.1.61
- GO:   GO:0005737
- GO:   GO:0006935
- HAMAP:   MF_00099
- InterPro:   IPR011006
- InterPro:   IPR008248
- InterPro:   IPR000673
- InterPro:   IPR001789
- Gene3D:   G3DSA:3.40.50.180
- PIRSF:   PIRSF000876
- SMART:   SM00448

Pfam domain/function: PF01339 CheB_methylest; PF00072 Response_reg; SSF52738 Chemotax_RR_pGlu_Me-esterase; SSF52172 CheY_like

EC number: =3.1.1.61

Molecular weight: Translated: 39095; Mature: 39095

Theoretical pI: Translated: 6.39; Mature: 6.39

Prosite motif: PS50122 CHEB; PS50110 RESPONSE_REGULATORY; PS00120 LIPASE_SER

Important sites: ACT_SITE 167-167 ACT_SITE 194-194 ACT_SITE 291-291

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
5.0 %Met     (Translated Protein)
7.0 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
5.0 %Met     (Mature Protein)
7.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKILVVDDSALMRTTISDILQNIPNAEIKTARDGMDAIDKVMKWQPDVMTLDINMPNMDG
CEEEEECCHHHHHHHHHHHHHHCCCCCCHHHHCCHHHHHHHHCCCCCEEEEEECCCCCCH
LTCLTQIMVERPLPIVMLSSLTHEGAITTLEALYLGAVDFVAKPGGTICGRLQEVAPLIR
HHHHHHHHHHCCCCEEEHHHCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
EKVRAAAKMRVKVHTQLSVSPAEVEHKHKSPVVENNEDHQLAIMGVSTGGPGTVETILRH
HHHHHHHHEEEEEEEEECCCHHHHHHHCCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHH
LPADFSLPIIVNQHMPESFTAAFAQRLNRHLQQPVKEINRALVLEAGTVYVCKGDRDCIV
CCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCEEEEEECCCCEEE
TLRDGKLAAMPTPNDSHFSWHPSVSKLVASAIRTCGEDNLLCVMLTGMGDDGATEMAQVA
EEECCEEEECCCCCCCCEEECCCHHHHHHHHHHHCCCCCEEEEEEECCCCCCHHHHHHHH
QGNGIVFAQEPTTCVVPSMPEALLKRVPHIPTGTPEHLAYLMTEVVRQSSRELHYGNH
CCCCEEEECCCCEEECCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MKILVVDDSALMRTTISDILQNIPNAEIKTARDGMDAIDKVMKWQPDVMTLDINMPNMDG
CEEEEECCHHHHHHHHHHHHHHCCCCCCHHHHCCHHHHHHHHCCCCCEEEEEECCCCCCH
LTCLTQIMVERPLPIVMLSSLTHEGAITTLEALYLGAVDFVAKPGGTICGRLQEVAPLIR
HHHHHHHHHHCCCCEEEHHHCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
EKVRAAAKMRVKVHTQLSVSPAEVEHKHKSPVVENNEDHQLAIMGVSTGGPGTVETILRH
HHHHHHHHEEEEEEEEECCCHHHHHHHCCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHH
LPADFSLPIIVNQHMPESFTAAFAQRLNRHLQQPVKEINRALVLEAGTVYVCKGDRDCIV
CCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCEEEEEECCCCEEE
TLRDGKLAAMPTPNDSHFSWHPSVSKLVASAIRTCGEDNLLCVMLTGMGDDGATEMAQVA
EEECCEEEECCCCCCCCEEECCCHHHHHHHHHHHCCCCCEEEEEEECCCCCCHHHHHHHH
QGNGIVFAQEPTTCVVPSMPEALLKRVPHIPTGTPEHLAYLMTEVVRQSSRELHYGNH
CCCCEEEECCCCEEECCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10952301