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The map label for this gene is cheW [H]

Identifier: 227081573

GI number: 227081573

Start: 1449748

End: 1451310

Strand: Reverse

Name: cheW [H]

Synonym: VCM66_1357

Alternate gene names: 227081573

Gene position: 1451310-1449748 (Counterclockwise)

Preceding gene: 227081574

Following gene: 227081572

Centisome position: 50.17

GC content: 44.47

Gene sequence:

>1563_bases
ATGACCAGTGAAGCCCTTGTAGACAACGAACTTGAGCATGAAGAGAACCTCACACAGTATGTCAATTTCATGCTGGGTGA
TGAGCTTTTTGCTTTACACATGCAAGAAGTGGAAGAAATTATCCGTCTCCCAACTACGTTTTCAGTACCACTCACTCCCA
GTTATTTGATTGGATTATCCAACTTAAGAGGACAAATTCTTCCCGTTCTGTGTCTACGGACTTTACTGTGCATGGACAGA
ACCTCTGCGACGGAAGCCACCCGAGTGATTGTCATTACAATTGGCAAAACCAAGATTGGATTCACCGTCGATCGTGTTGT
GAATGTGGCAACCCCTGACCCTGAGATGATAAAGCCGACCAATACCGCGGGAGGCAAAATTGACCCAACCTTAATTACCA
ACACCATTCACTCGGATAACCAGATTATCCAAGTACTGAATTGTCATCGTTTGTTAGACAACTCTGTCGAAGAAGAATTG
GAATTAAGCACGCAGCGTTTTAACCACCTCTCTTCTCAGTCTCATTTTGGGGATGTGATCGATGAAGAACGAGATGATGA
TGAAGACATGCGACAGCTCGTGTGCTGCATGGTAGATGGGCAAGAATACGCCTTTCCACTAGAGGACACTCAAGAAATTG
TGCGTATACCCGAGATTATCACCAAGGTCCCTCTTACTGAATCCGCCATTCTTGGAGTGATCAATTTACGTGGCAAAACG
CTCCCATTGGTCAGTCTGAGAACGCTGTTTGGATTACCTAGCATTAGCTTTAGTGATAGTCACCGTGTCTTGGTGGTGAA
TATATCCCTCTCAGAGAAAGAGCGGCTTCCCGTGGGGGTTGTTGTGGATGCCGTACGCGAAGTCATACGTCTGCATGTAG
ACGTGATGGATAGCGTTCCAGAAATCATGAGTAAAATGGGACAAGCCAATGACATTTCGGCCATTTGTAATCTTGATGAT
GGCCGACGCACACTCAGCGTTATTTCTGTAGAGCAACTTTTTGACGCTCAAATCGTAGATCAATTCTCCGAAGCTTACCC
ACAGGAAGGAACTGATATGTCGACGATCGACGATGTAAGTATCCTAGAAGATGACAATACCGACATGCAGTTGGTGATTT
TTAAGTTGGATAAAGAAGAATTTGGCATCTCCATTCATACCGTACAAGAAATTATCCGTATTCCGGAAAACATCAGCCGT
GTTCCTAAAACCGATGATTTTATCGAAGGAGTAATTAATTTACGCGGTAACGTTTTACCTATCGTCGATATGCGTAAGCG
CTTTCATCTACCCGAGATGGCACGCCATGAACGTCAACGTATCTTAGTTGTGAATTTTGAGAAGATTTCAACAGGTTTTA
TCGTCGATTCCGTCAGTGAAGTATTAAGAATACCGGAATCCCAGCTGGAAGATGCGCCCGTGTTGAGCGAAGAGCAAGCA
CAACTAATGCGCCAAATGGTCAATCTCAGCCCTCGAATGATAGGAGTACTGTCCGCCGATCAATTGATCAGTAATACCGA
GATGTACAAGTTACACATGGCTGCAAACGATATGGATAGCTAA

Upstream 100 bases:

>100_bases
GGCTCGTCAGCAAAGCTCAAGTACTTCGGAGCTGGCATCCGCAATTGAAAATATTGCCGCCGTTGCCGATGAACTTCAAT
CAATCTGATTGAGGTGAACT

Downstream 100 bases:

>100_bases
GTTTTAAGGCTGGAGGGAACATGAAGATTTTAGTTGTCGATGATTCTGCGTTAATGCGCACCACCATTAGCGACATATTG
CAAAACATTCCGAATGCCGA

Product: putative purine-binding chemotaxis protein Chew

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 520; Mature: 519

Protein sequence:

>520_residues
MTSEALVDNELEHEENLTQYVNFMLGDELFALHMQEVEEIIRLPTTFSVPLTPSYLIGLSNLRGQILPVLCLRTLLCMDR
TSATEATRVIVITIGKTKIGFTVDRVVNVATPDPEMIKPTNTAGGKIDPTLITNTIHSDNQIIQVLNCHRLLDNSVEEEL
ELSTQRFNHLSSQSHFGDVIDEERDDDEDMRQLVCCMVDGQEYAFPLEDTQEIVRIPEIITKVPLTESAILGVINLRGKT
LPLVSLRTLFGLPSISFSDSHRVLVVNISLSEKERLPVGVVVDAVREVIRLHVDVMDSVPEIMSKMGQANDISAICNLDD
GRRTLSVISVEQLFDAQIVDQFSEAYPQEGTDMSTIDDVSILEDDNTDMQLVIFKLDKEEFGISIHTVQEIIRIPENISR
VPKTDDFIEGVINLRGNVLPIVDMRKRFHLPEMARHERQRILVVNFEKISTGFIVDSVSEVLRIPESQLEDAPVLSEEQA
QLMRQMVNLSPRMIGVLSADQLISNTEMYKLHMAANDMDS

Sequences:

>Translated_520_residues
MTSEALVDNELEHEENLTQYVNFMLGDELFALHMQEVEEIIRLPTTFSVPLTPSYLIGLSNLRGQILPVLCLRTLLCMDR
TSATEATRVIVITIGKTKIGFTVDRVVNVATPDPEMIKPTNTAGGKIDPTLITNTIHSDNQIIQVLNCHRLLDNSVEEEL
ELSTQRFNHLSSQSHFGDVIDEERDDDEDMRQLVCCMVDGQEYAFPLEDTQEIVRIPEIITKVPLTESAILGVINLRGKT
LPLVSLRTLFGLPSISFSDSHRVLVVNISLSEKERLPVGVVVDAVREVIRLHVDVMDSVPEIMSKMGQANDISAICNLDD
GRRTLSVISVEQLFDAQIVDQFSEAYPQEGTDMSTIDDVSILEDDNTDMQLVIFKLDKEEFGISIHTVQEIIRIPENISR
VPKTDDFIEGVINLRGNVLPIVDMRKRFHLPEMARHERQRILVVNFEKISTGFIVDSVSEVLRIPESQLEDAPVLSEEQA
QLMRQMVNLSPRMIGVLSADQLISNTEMYKLHMAANDMDS
>Mature_519_residues
TSEALVDNELEHEENLTQYVNFMLGDELFALHMQEVEEIIRLPTTFSVPLTPSYLIGLSNLRGQILPVLCLRTLLCMDRT
SATEATRVIVITIGKTKIGFTVDRVVNVATPDPEMIKPTNTAGGKIDPTLITNTIHSDNQIIQVLNCHRLLDNSVEEELE
LSTQRFNHLSSQSHFGDVIDEERDDDEDMRQLVCCMVDGQEYAFPLEDTQEIVRIPEIITKVPLTESAILGVINLRGKTL
PLVSLRTLFGLPSISFSDSHRVLVVNISLSEKERLPVGVVVDAVREVIRLHVDVMDSVPEIMSKMGQANDISAICNLDDG
RRTLSVISVEQLFDAQIVDQFSEAYPQEGTDMSTIDDVSILEDDNTDMQLVIFKLDKEEFGISIHTVQEIIRIPENISRV
PKTDDFIEGVINLRGNVLPIVDMRKRFHLPEMARHERQRILVVNFEKISTGFIVDSVSEVLRIPESQLEDAPVLSEEQAQ
LMRQMVNLSPRMIGVLSADQLISNTEMYKLHMAANDMDS

Specific function: Involved in the transmission of sensory signals from the chemoreceptors to the flagellar motors. It physically bridges CheA to the MCPs (methyl-accepting chemotaxis proteins) to allow regulated phosphotransfer to CheY and CheB [H]

COG id: COG0835

COG function: function code NT; Chemotaxis signal transduction protein

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 cheW-like domain [H]

Homologues:

Organism=Escherichia coli, GI1788196, Length=167, Percent_Identity=31.1377245508982, Blast_Score=89, Evalue=6e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002545 [H]

Pfam domain/function: PF01584 CheW [H]

EC number: NA

Molecular weight: Translated: 58516; Mature: 58385

Theoretical pI: Translated: 4.30; Mature: 4.30

Prosite motif: PS50851 CHEW

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSEALVDNELEHEENLTQYVNFMLGDELFALHMQEVEEIIRLPTTFSVPLTPSYLIGLS
CCCCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCHHHHHHHH
NLRGQILPVLCLRTLLCMDRTSATEATRVIVITIGKTKIGFTVDRVVNVATPDPEMIKPT
HCCHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEECCCEECEEHHHEEEECCCCCCCCCCC
NTAGGKIDPTLITNTIHSDNQIIQVLNCHRLLDNSVEEELELSTQRFNHLSSQSHFGDVI
CCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCHHH
DEERDDDEDMRQLVCCMVDGQEYAFPLEDTQEIVRIPEIITKVPLTESAILGVINLRGKT
CCCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHEEEEECCCCC
LPLVSLRTLFGLPSISFSDSHRVLVVNISLSEKERLPVGVVVDAVREVIRLHVDVMDSVP
CCHHHHHHHHCCCCCCCCCCCEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
EIMSKMGQANDISAICNLDDGRRTLSVISVEQLFDAQIVDQFSEAYPQEGTDMSTIDDVS
HHHHHCCCCCCCEEEEECCCCCHHHHHEEHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCE
ILEDDNTDMQLVIFKLDKEEFGISIHTVQEIIRIPENISRVPKTDDFIEGVINLRGNVLP
EEECCCCCEEEEEEEECHHHHCEEHHHHHHHHHCCHHHHCCCCCHHHHHHHHHCCCCEEE
IVDMRKRFHLPEMARHERQRILVVNFEKISTGFIVDSVSEVLRIPESQLEDAPVLSEEQA
HHHHHHHCCCCHHHHHCCCEEEEEEEHHCCCCHHHHHHHHHHHCCHHHCCCCCCCCHHHH
QLMRQMVNLSPRMIGVLSADQLISNTEMYKLHMAANDMDS
HHHHHHHCCCCCEEEEECHHHHHCCCCEEEEEECCCCCCC
>Mature Secondary Structure 
TSEALVDNELEHEENLTQYVNFMLGDELFALHMQEVEEIIRLPTTFSVPLTPSYLIGLS
CCCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCHHHHHHHH
NLRGQILPVLCLRTLLCMDRTSATEATRVIVITIGKTKIGFTVDRVVNVATPDPEMIKPT
HCCHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEECCCEECEEHHHEEEECCCCCCCCCCC
NTAGGKIDPTLITNTIHSDNQIIQVLNCHRLLDNSVEEELELSTQRFNHLSSQSHFGDVI
CCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCHHH
DEERDDDEDMRQLVCCMVDGQEYAFPLEDTQEIVRIPEIITKVPLTESAILGVINLRGKT
CCCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHEEEEECCCCC
LPLVSLRTLFGLPSISFSDSHRVLVVNISLSEKERLPVGVVVDAVREVIRLHVDVMDSVP
CCHHHHHHHHCCCCCCCCCCCEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
EIMSKMGQANDISAICNLDDGRRTLSVISVEQLFDAQIVDQFSEAYPQEGTDMSTIDDVS
HHHHHCCCCCCCEEEEECCCCCHHHHHEEHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCE
ILEDDNTDMQLVIFKLDKEEFGISIHTVQEIIRIPENISRVPKTDDFIEGVINLRGNVLP
EEECCCCCEEEEEEEECHHHHCEEHHHHHHHHHCCHHHHCCCCCHHHHHHHHHCCCCEEE
IVDMRKRFHLPEMARHERQRILVVNFEKISTGFIVDSVSEVLRIPESQLEDAPVLSEEQA
HHHHHHHCCCCHHHHHCCCEEEEEEEHHCCCCHHHHHHHHHHHCCHHHCCCCCCCCHHHH
QLMRQMVNLSPRMIGVLSADQLISNTEMYKLHMAANDMDS
HHHHHHHCCCCCEEEEECHHHHHCCCCEEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]