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The map label for this gene is nreB [H]

Identifier: 227081448

GI number: 227081448

Start: 1308215

End: 1309570

Strand: Direct

Name: nreB [H]

Synonym: VCM66_1231

Alternate gene names: 227081448

Gene position: 1308215-1309570 (Clockwise)

Preceding gene: 227081447

Following gene: 227081449

Centisome position: 45.23

GC content: 46.61

Gene sequence:

>1356_bases
ATGCCTCTCAAAGCCAAATTGATTTTGCTGGCTTTACTGCCTCTCTTAATTGCCACAGCAAGCATCAGTTGGATTTCGAT
CCATCAGGCTAAAACCTTAGGCCAGCGTGAAATTGAGATCTTCCGAGCTAAGCTGATGGAAGAGCGCGAAACCTCACTCA
AAGACAGCGTCGAGGTGGCGTTTGATGCGGTGAGGTTGATTGCCAATAATCCCAACTTAAGTGAAGCGGAAGCCAAACAG
CAGGTCAAAGCAGTATTAACCAATATTCGTTATGGATCTGATGGCTATTTTTTCGCTTATGATGCTCAGGGCACGAACTT
GGTGCATCCGATCCAGCCTGAGCTGGTAGGGCAAAACTTATGGCAGTTGCAAGATGAACGCGGTGACTTTCTCATCCAAG
CGCTACTTTTCCAAGCCCAATCGGGTGGTGGGTATCACCAATATTTGTGGCGCAAACCCTCAAGTGGAGAAACTGTCCCT
AAACTCAGCTATTCGGCTTGGCTTGACGACTGGGAATGGATGATCGGCACTGGCTTGTACATTGAAGATCTCAGTCATGA
AGTCGAGAAGATGCAAGCGGCAGTGAACGCAAATATTGATACCACCTTCTTTTCTGTTGTGGTGATTTTGATAGTCACTG
TAGCGGTGATCATTGTGTTGACCTTAGCCATCAACCTGCATGAACACCGTTTGGCGGATAAGAATCTCAAAGATTTGGTA
CACAAAACCGTGTTGTTTCAGGAAGATGAAAAGAAGCATCTCGCCCGCGAACTGCATGACGGAATTAATCAGTTATTGGT
GTCTAGCAAGTGTCATCTTGAGTTACTGGCCAACCAACTGAACGACCCTAAACAGATCGTGCATCTGGAAAAATCCCAGC
ACTCGTTGATGACTGCAATTAATGAAGTTCGGCGAATTTCACACCATTTGCGCCCCAGTGCGCTGGATGATCTCGGTTTA
CAAGCGGCACTCACGACGCTGCTCGAGGATTTCAAACTGCATTCACAAATGGAGATCGAGATGTTGTTTGACACCCAACC
CGGCAAGCTAAAATCGGAAGTGGCCACCACGTTGTATCGAGTGGTGCAAGAGTCACTCAATAATATTGAAAAACACGCCA
AAGCCGAGAAAGTCACGGTGTTACTGCAGCAAATGGGTGATATGTTGCAGCTTATGGTGCGTGATGATGGGGTAGGATTT
TCCAGCAAAGAAGCGCTGCAAAAACGCGGGATTGGTTTACGTAACATGCGCGAACGTGTGGAGTTTATTGGTGGGGAATT
TGAATTAAGCAGCGAGCCACAATTGGGCACCGAAATTACGGTTTTACTGAAGTTGGATGGAAGTGTTTATGGATAA

Upstream 100 bases:

>100_bases
TCAAGAGTGAGCGTATCGAAATTCAATCCACAACAACAAGTTGTTAGAGTGAAACTGCTTTTCGAAGGGTCGGAAAGCAG
TTTTTTTATGGGGAAATGGA

Downstream 100 bases:

>100_bases
ACCAATCAGAGTGGTGATGGTGGATGATCATCAGGTGGTGTTGGATGGTTTTATTGCGCGTTTAGAGCAAGAGCCGGAAA
TCGAGGTCGTCGCCACCGCG

Product: sensor histidine kinase

Products: NA

Alternate protein names: Nitrogen regulation protein B [H]

Number of amino acids: Translated: 451; Mature: 450

Protein sequence:

>451_residues
MPLKAKLILLALLPLLIATASISWISIHQAKTLGQREIEIFRAKLMEERETSLKDSVEVAFDAVRLIANNPNLSEAEAKQ
QVKAVLTNIRYGSDGYFFAYDAQGTNLVHPIQPELVGQNLWQLQDERGDFLIQALLFQAQSGGGYHQYLWRKPSSGETVP
KLSYSAWLDDWEWMIGTGLYIEDLSHEVEKMQAAVNANIDTTFFSVVVILIVTVAVIIVLTLAINLHEHRLADKNLKDLV
HKTVLFQEDEKKHLARELHDGINQLLVSSKCHLELLANQLNDPKQIVHLEKSQHSLMTAINEVRRISHHLRPSALDDLGL
QAALTTLLEDFKLHSQMEIEMLFDTQPGKLKSEVATTLYRVVQESLNNIEKHAKAEKVTVLLQQMGDMLQLMVRDDGVGF
SSKEALQKRGIGLRNMRERVEFIGGEFELSSEPQLGTEITVLLKLDGSVYG

Sequences:

>Translated_451_residues
MPLKAKLILLALLPLLIATASISWISIHQAKTLGQREIEIFRAKLMEERETSLKDSVEVAFDAVRLIANNPNLSEAEAKQ
QVKAVLTNIRYGSDGYFFAYDAQGTNLVHPIQPELVGQNLWQLQDERGDFLIQALLFQAQSGGGYHQYLWRKPSSGETVP
KLSYSAWLDDWEWMIGTGLYIEDLSHEVEKMQAAVNANIDTTFFSVVVILIVTVAVIIVLTLAINLHEHRLADKNLKDLV
HKTVLFQEDEKKHLARELHDGINQLLVSSKCHLELLANQLNDPKQIVHLEKSQHSLMTAINEVRRISHHLRPSALDDLGL
QAALTTLLEDFKLHSQMEIEMLFDTQPGKLKSEVATTLYRVVQESLNNIEKHAKAEKVTVLLQQMGDMLQLMVRDDGVGF
SSKEALQKRGIGLRNMRERVEFIGGEFELSSEPQLGTEITVLLKLDGSVYG
>Mature_450_residues
PLKAKLILLALLPLLIATASISWISIHQAKTLGQREIEIFRAKLMEERETSLKDSVEVAFDAVRLIANNPNLSEAEAKQQ
VKAVLTNIRYGSDGYFFAYDAQGTNLVHPIQPELVGQNLWQLQDERGDFLIQALLFQAQSGGGYHQYLWRKPSSGETVPK
LSYSAWLDDWEWMIGTGLYIEDLSHEVEKMQAAVNANIDTTFFSVVVILIVTVAVIIVLTLAINLHEHRLADKNLKDLVH
KTVLFQEDEKKHLARELHDGINQLLVSSKCHLELLANQLNDPKQIVHLEKSQHSLMTAINEVRRISHHLRPSALDDLGLQ
AALTTLLEDFKLHSQMEIEMLFDTQPGKLKSEVATTLYRVVQESLNNIEKHAKAEKVTVLLQQMGDMLQLMVRDDGVGFS
SKEALQKRGIGLRNMRERVEFIGGEFELSSEPQLGTEITVLLKLDGSVYG

Specific function: Member of the two-component regulatory system nreB/nreC involved in the control of dissimilatory nitrate/nitrite reduction in response to oxygen. NreB functions as a direct oxygen sensor histidine kinase which is autophosphorylated, in the absence of oxyg

COG id: COG4564

COG function: function code T; Signal transduction histidine kinase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI87082311, Length=200, Percent_Identity=32.5, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI1787474, Length=239, Percent_Identity=26.3598326359833, Blast_Score=69, Evalue=9e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR004358
- InterPro:   IPR011712
- InterPro:   IPR005467
- InterPro:   IPR017203 [H]

Pfam domain/function: PF02518 HATPase_c; PF07730 HisKA_3 [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 50824; Mature: 50693

Theoretical pI: Translated: 5.84; Mature: 5.84

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPLKAKLILLALLPLLIATASISWISIHQAKTLGQREIEIFRAKLMEERETSLKDSVEVA
CCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHH
FDAVRLIANNPNLSEAEAKQQVKAVLTNIRYGSDGYFFAYDAQGTNLVHPIQPELVGQNL
HHHHHHHHCCCCCCHHHHHHHHHHHHHHCEECCCCEEEEEECCCCCCCCCCCHHHHCCHH
WQLQDERGDFLIQALLFQAQSGGGYHQYLWRKPSSGETVPKLSYSAWLDDWEWMIGTGLY
HHHHHCCHHHHHHHHHHHHCCCCCHHHHHEECCCCCCCCCCCHHHHHHCCHHHHHCCCHH
IEDLSHEVEKMQAAVNANIDTTFFSVVVILIVTVAVIIVLTLAINLHEHRLADKNLKDLV
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHH
HKTVLFQEDEKKHLARELHDGINQLLVSSKCHLELLANQLNDPKQIVHLEKSQHSLMTAI
HHHHHHCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
NEVRRISHHLRPSALDDLGLQAALTTLLEDFKLHSQMEIEMLFDTQPGKLKSEVATTLYR
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEEEEECCCCCHHHHHHHHHHHH
VVQESLNNIEKHAKAEKVTVLLQQMGDMLQLMVRDDGVGFSSKEALQKRGIGLRNMRERV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCHHHHHHHH
EFIGGEFELSSEPQLGTEITVLLKLDGSVYG
HHCCCCEEECCCCCCCCEEEEEEEECCCCCC
>Mature Secondary Structure 
PLKAKLILLALLPLLIATASISWISIHQAKTLGQREIEIFRAKLMEERETSLKDSVEVA
CCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHH
FDAVRLIANNPNLSEAEAKQQVKAVLTNIRYGSDGYFFAYDAQGTNLVHPIQPELVGQNL
HHHHHHHHCCCCCCHHHHHHHHHHHHHHCEECCCCEEEEEECCCCCCCCCCCHHHHCCHH
WQLQDERGDFLIQALLFQAQSGGGYHQYLWRKPSSGETVPKLSYSAWLDDWEWMIGTGLY
HHHHHCCHHHHHHHHHHHHCCCCCHHHHHEECCCCCCCCCCCHHHHHHCCHHHHHCCCHH
IEDLSHEVEKMQAAVNANIDTTFFSVVVILIVTVAVIIVLTLAINLHEHRLADKNLKDLV
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHH
HKTVLFQEDEKKHLARELHDGINQLLVSSKCHLELLANQLNDPKQIVHLEKSQHSLMTAI
HHHHHHCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
NEVRRISHHLRPSALDDLGLQAALTTLLEDFKLHSQMEIEMLFDTQPGKLKSEVATTLYR
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEEEEECCCCCHHHHHHHHHHHH
VVQESLNNIEKHAKAEKVTVLLQQMGDMLQLMVRDDGVGFSSKEALQKRGIGLRNMRERV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCHHHHHHHH
EFIGGEFELSSEPQLGTEITVLLKLDGSVYG
HHCCCCEEECCCCCCCCEEEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12426351 [H]