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The map label for this gene is nreC [H]

Identifier: 227081449

GI number: 227081449

Start: 1309551

End: 1310201

Strand: Direct

Name: nreC [H]

Synonym: VCM66_1232

Alternate gene names: 227081449

Gene position: 1309551-1310201 (Clockwise)

Preceding gene: 227081448

Following gene: 227081451

Centisome position: 45.27

GC content: 47.31

Gene sequence:

>651_bases
ATGGAAGTGTTTATGGATAAACCAATCAGAGTGGTGATGGTGGATGATCATCAGGTGGTGTTGGATGGTTTTATTGCGCG
TTTAGAGCAAGAGCCGGAAATCGAGGTCGTCGCCACCGCGAGTAATGGATTGGAAGCTTTAGAATTGGTCAAACTTCACC
AACCGGATGTGGTGCTGATGGATGTGAGTATGCCGATCATGAACGGTATTGAAGCCACCCGCTTGATCAAAGAAGAAGTT
CCTCACACCAAAGTATTGATGCTGACCATGCATGATAACCGCGAGTACATTATGCAAGTGATGCAAGCCGGCGCAATGGG
CTACATGCTCAAAGAAATCTCAGCACTGAAAATGGTGCAAGCGATCAAAACGGTTCATCAAGGCGCGACGTACTTCTGCG
AATCCACCACCTCGACACTGTTTAGCCAAGAGATTTTACCTGTGGCGAATAAGCCAAACCCGCTGAGCCGCCGTGAAGAG
GCAATTTTACGTATGGTTGCACAAGGTTGCAGCAGTAAAAAAATCGCGACCTTGCTGAATATCAGTTATCGCACGGTCGA
AACCCATAGGCAAAATATTAAGCATAAGCTCGACATCCACTCCACGGCAGAGTTAGCTCAATACGCGCTACAACATGGTT
TACTGGAGTGA

Upstream 100 bases:

>100_bases
TTACGTAACATGCGCGAACGTGTGGAGTTTATTGGTGGGGAATTTGAATTAAGCAGCGAGCCACAATTGGGCACCGAAAT
TACGGTTTTACTGAAGTTGG

Downstream 100 bases:

>100_bases
ACTATTCTGGCTGCGTGATGCTACCAATCGTCAGCATCGGCGCGGCATCCATATCTTGCGCGTTCATCATGGCTGAGATG
CGTTGTACAGAAGAGAGCAG

Product: transcriptional regulator, LuxR family

Products: NA

Alternate protein names: Nitrogen regulation protein C [H]

Number of amino acids: Translated: 216; Mature: 216

Protein sequence:

>216_residues
MEVFMDKPIRVVMVDDHQVVLDGFIARLEQEPEIEVVATASNGLEALELVKLHQPDVVLMDVSMPIMNGIEATRLIKEEV
PHTKVLMLTMHDNREYIMQVMQAGAMGYMLKEISALKMVQAIKTVHQGATYFCESTTSTLFSQEILPVANKPNPLSRREE
AILRMVAQGCSSKKIATLLNISYRTVETHRQNIKHKLDIHSTAELAQYALQHGLLE

Sequences:

>Translated_216_residues
MEVFMDKPIRVVMVDDHQVVLDGFIARLEQEPEIEVVATASNGLEALELVKLHQPDVVLMDVSMPIMNGIEATRLIKEEV
PHTKVLMLTMHDNREYIMQVMQAGAMGYMLKEISALKMVQAIKTVHQGATYFCESTTSTLFSQEILPVANKPNPLSRREE
AILRMVAQGCSSKKIATLLNISYRTVETHRQNIKHKLDIHSTAELAQYALQHGLLE
>Mature_216_residues
MEVFMDKPIRVVMVDDHQVVLDGFIARLEQEPEIEVVATASNGLEALELVKLHQPDVVLMDVSMPIMNGIEATRLIKEEV
PHTKVLMLTMHDNREYIMQVMQAGAMGYMLKEISALKMVQAIKTVHQGATYFCESTTSTLFSQEILPVANKPNPLSRREE
AILRMVAQGCSSKKIATLLNISYRTVETHRQNIKHKLDIHSTAELAQYALQHGLLE

Specific function: Member of the two-component regulatory system nreB/nreC involved in the control of dissimilatory nitrate/nitrite reduction in response to oxygen. Phosphorylated nreC binds to a GC-rich palindromic sequence at the promoters of the nitrate (narGHJI) and nit

COG id: COG2197

COG function: function code TK; Response regulator containing a CheY-like receiver domain and an HTH DNA-binding domain

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1788222, Length=208, Percent_Identity=37.5, Blast_Score=132, Evalue=2e-32,
Organism=Escherichia coli, GI1788521, Length=195, Percent_Identity=33.3333333333333, Blast_Score=117, Evalue=5e-28,
Organism=Escherichia coli, GI1790102, Length=199, Percent_Identity=33.1658291457286, Blast_Score=113, Evalue=8e-27,
Organism=Escherichia coli, GI1786747, Length=203, Percent_Identity=29.064039408867, Blast_Score=98, Evalue=5e-22,
Organism=Escherichia coli, GI1787473, Length=189, Percent_Identity=30.1587301587302, Blast_Score=87, Evalue=9e-19,
Organism=Escherichia coli, GI1788712, Length=198, Percent_Identity=25.7575757575758, Blast_Score=82, Evalue=4e-17,
Organism=Escherichia coli, GI1788546, Length=203, Percent_Identity=26.1083743842365, Blast_Score=74, Evalue=8e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR016032
- InterPro:   IPR001789
- InterPro:   IPR000792
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00196 GerE; PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 24351; Mature: 24351

Theoretical pI: Translated: 6.23; Mature: 6.23

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00622 HTH_LUXR_1 ; PS50043 HTH_LUXR_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
6.5 %Met     (Translated Protein)
7.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
6.5 %Met     (Mature Protein)
7.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEVFMDKPIRVVMVDDHQVVLDGFIARLEQEPEIEVVATASNGLEALELVKLHQPDVVLM
CCCCCCCCEEEEEECCCHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHCCCCEEEE
DVSMPIMNGIEATRLIKEEVPHTKVLMLTMHDNREYIMQVMQAGAMGYMLKEISALKMVQ
ECCCHHHCCHHHHHHHHHHCCCEEEEEEEEECCHHHHHHHHHHCHHHHHHHHHHHHHHHH
AIKTVHQGATYFCESTTSTLFSQEILPVANKPNPLSRREEAILRMVAQGCSSKKIATLLN
HHHHHHCCCCEEEHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHH
ISYRTVETHRQNIKHKLDIHSTAELAQYALQHGLLE
HHHHHHHHHHHCCHHHHCCCHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MEVFMDKPIRVVMVDDHQVVLDGFIARLEQEPEIEVVATASNGLEALELVKLHQPDVVLM
CCCCCCCCEEEEEECCCHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHCCCCEEEE
DVSMPIMNGIEATRLIKEEVPHTKVLMLTMHDNREYIMQVMQAGAMGYMLKEISALKMVQ
ECCCHHHCCHHHHHHHHHHCCCEEEEEEEEECCHHHHHHHHHHCHHHHHHHHHHHHHHHH
AIKTVHQGATYFCESTTSTLFSQEILPVANKPNPLSRREEAILRMVAQGCSSKKIATLLN
HHHHHHCCCCEEEHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHH
ISYRTVETHRQNIKHKLDIHSTAELAQYALQHGLLE
HHHHHHHHHHHCCHHHHCCCHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12426351 [H]