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The map label for this gene is cspD

Identifier: 227081315

GI number: 227081315

Start: 1169414

End: 1169644

Strand: Reverse

Name: cspD

Synonym: VCM66_1098

Alternate gene names: 227081315

Gene position: 1169644-1169414 (Counterclockwise)

Preceding gene: 227081318

Following gene: 227081313

Centisome position: 40.44

GC content: 45.89

Gene sequence:

>231_bases
ATGTATAGCATGGCTACAGGTACAGTGAAATGGTTTAATAATGCCAAAGGCTTCGGTTTTATTTGCCCGGAAGGTGAAGA
CGGAGATATTTTCGCTCATTACTCAACCATTCAGATGGACGGATACCGTACACTCAAAGCCGGTCAACAGGTTTCTTATC
AGGTGGAGCAAGGTCCTAAGGGATACCACGCGAGCTGTGTTGTGCCAATCGAGGGACAATCAGCAAAATAG

Upstream 100 bases:

>100_bases
TTAAGCATAGGCTGAATCGCTATTCACTTTTGAGAAGTCAGGAAGTCCTAGGGACAAATTGACGTTTCTCTTAAAACATC
AGTTATAAAATGCATGAGGG

Downstream 100 bases:

>100_bases
TGGATTGATTGCCACACTAGCGTGCTTTGGCAATTTGCAAAGCACAAAAATAGAAACCCGCCCAATTGGGCGGGTTTTGT
TTTGGTGTCTAAACTAGATT

Product: cold shock-like protein CspD

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 76; Mature: 76

Protein sequence:

>76_residues
MYSMATGTVKWFNNAKGFGFICPEGEDGDIFAHYSTIQMDGYRTLKAGQQVSYQVEQGPKGYHASCVVPIEGQSAK

Sequences:

>Translated_76_residues
MYSMATGTVKWFNNAKGFGFICPEGEDGDIFAHYSTIQMDGYRTLKAGQQVSYQVEQGPKGYHASCVVPIEGQSAK
>Mature_76_residues
MYSMATGTVKWFNNAKGFGFICPEGEDGDIFAHYSTIQMDGYRTLKAGQQVSYQVEQGPKGYHASCVVPIEGQSAK

Specific function: Inhibits DNA Replication At Both Initiation And Elongation Steps, Most Probably By Binding To The Opened, Single- Stranded Regions At Replication Forks. Plays A Regulatory Role In Chromosomal Replication In Nutrient-Depleted Cells. [C]

COG id: COG1278

COG function: function code K; Cold shock proteins

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CSD (cold-shock) domain

Homologues:

Organism=Escherichia coli, GI1787107, Length=72, Percent_Identity=76.3888888888889, Blast_Score=119, Evalue=4e-29,
Organism=Escherichia coli, GI1789979, Length=70, Percent_Identity=50, Blast_Score=77, Evalue=3e-16,
Organism=Escherichia coli, GI1786841, Length=68, Percent_Identity=47.0588235294118, Blast_Score=76, Evalue=4e-16,
Organism=Escherichia coli, GI1787839, Length=68, Percent_Identity=51.4705882352941, Blast_Score=76, Evalue=5e-16,
Organism=Escherichia coli, GI2367114, Length=70, Percent_Identity=45.7142857142857, Blast_Score=74, Evalue=2e-15,
Organism=Escherichia coli, GI1787834, Length=70, Percent_Identity=47.1428571428571, Blast_Score=73, Evalue=3e-15,
Organism=Escherichia coli, GI1788126, Length=64, Percent_Identity=48.4375, Blast_Score=70, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): CSPD_VIBCH (Q9KSW4)

Other databases:

- EMBL:   AE003852
- PIR:   C82236
- RefSeq:   NP_230787.1
- ProteinModelPortal:   Q9KSW4
- SMR:   Q9KSW4
- GeneID:   2614575
- GenomeReviews:   AE003852_GR
- KEGG:   vch:VC1142
- TIGR:   VC_1142
- HOGENOM:   HBG717773
- OMA:   QVVRFDV
- ProtClustDB:   CLSK793973
- GO:   GO:0005737
- GO:   GO:0006350
- InterPro:   IPR019844
- InterPro:   IPR012156
- InterPro:   IPR011129
- InterPro:   IPR002059
- InterPro:   IPR012751
- InterPro:   IPR012340
- InterPro:   IPR016027
- Gene3D:   G3DSA:2.40.50.140
- PIRSF:   PIRSF002599
- PRINTS:   PR00050
- SMART:   SM00357
- TIGRFAMs:   TIGR02381

Pfam domain/function: PF00313 CSD; SSF50249 Nucleic_acid_OB

EC number: NA

Molecular weight: Translated: 8328; Mature: 8328

Theoretical pI: Translated: 6.50; Mature: 6.50

Prosite motif: PS00352 COLD_SHOCK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
6.6 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
6.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYSMATGTVKWFNNAKGFGFICPEGEDGDIFAHYSTIQMDGYRTLKAGQQVSYQVEQGPK
CCCCCCCEEEECCCCCCCEEECCCCCCCCEEEEEEEEEECCEEEECCCCEEEEEECCCCC
GYHASCVVPIEGQSAK
CCCCEEEEEECCCCCC
>Mature Secondary Structure
MYSMATGTVKWFNNAKGFGFICPEGEDGDIFAHYSTIQMDGYRTLKAGQQVSYQVEQGPK
CCCCCCCEEEECCCCCCCEEECCCCCCCCEEEEEEEEEECCEEEECCCCEEEEEECCCCC
GYHASCVVPIEGQSAK
CCCCEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10952301