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The map label for this gene is rluE [H]

Identifier: 227081313

GI number: 227081313

Start: 1166057

End: 1166929

Strand: Reverse

Name: rluE [H]

Synonym: VCM66_1096

Alternate gene names: 227081313

Gene position: 1166929-1166057 (Counterclockwise)

Preceding gene: 227081315

Following gene: 227081303

Centisome position: 40.34

GC content: 47.65

Gene sequence:

>873_bases
GTGAAGGTATTTATAATTAAACAAATTGAAGACAATAGTCTATTATCTTGTTCGTTAAACCTTTTCCAGGGCGCAAATGA
TAGCCGATCTCGCCTCTACGGAAAACTTTCAAACACAGTTTATTTACATTTTTGCAAGGTGCTTAACATGTCATCTCGCT
TGTCCAGCGATGCCACCTCCTCTTCTTCCGATCAACGAAAGTCGCATTTTAAACAAAGAGCCGCGAAAAACGCTGGCCAT
CCACCAACGAGAGCAAATCGAAAGAGTGTTGCTAACAAGAAAAAAAATGCCACTCAAACGGCTTTGTCACCTAAGCGCCC
CCTTTCTCCTGCTGAGCGTAAAGTGATCCTGTTTAATAAACCTTATGACACTTTAAGCCAATTTACCGATGGGGATGGAA
GAAAAACCTTGGCCGACTACATCCCAATTAAGGATGTTTACGCCGCAGGACGTTTGGATCGCGATAGCGAAGGACTACTG
ATTTTAACCAATGATGGAATTTTGCAGGCCAGATTGACTCAGCCGCAGTCCAAAGCGCCAAAAACTTACTGGGTGCAAGT
TGAAGGTTCGCCACAAGAGAGCGACCTTGAGGCATTGCGCCACGGAGTGACCTTAAAAGATGGCCCGACTTTACCGGCGA
AAGTCGACATCATGCCCGAACCCACGCTTTGGCCACGCAATCCGCCGGTGCGTTTTCGCGCAGCAATTCCGACCACATGG
TTAGCCATTACTTTAATGGAAGGGCGTAATCGCCAAGTACGACGCATGACGGCGCACATCGGTTTCCCGACTCTACGCTT
GATTCGTTACTCCATGGGCGATTGGAACTTGGGAGATTTGCAGCCCGGTGAGTGGCGGGAAGTGACGCTTTAA

Upstream 100 bases:

>100_bases
TTCTCAAGTCGGCGACATCCCCTCAACCTGAGTACGATCTTCTCACTCTTGCAAATCCTGTGTTCGAATGACGTTGAGCC
GCCTTCGAGCTCGTCCTTGA

Downstream 100 bases:

>100_bases
TACTCTGATTAATCAAAAATATATAGCCAAACTACTTGGAGTTGCAGGTAGGCGGCAAATGAGTGAATCCCCATGAGCAT
AGATACACTATGTGATTGGG

Product: pseudouridine synthase family 1 protein

Products: NA

Alternate protein names: rRNA pseudouridylate synthase E; rRNA-uridine isomerase E [H]

Number of amino acids: Translated: 290; Mature: 290

Protein sequence:

>290_residues
MKVFIIKQIEDNSLLSCSLNLFQGANDSRSRLYGKLSNTVYLHFCKVLNMSSRLSSDATSSSSDQRKSHFKQRAAKNAGH
PPTRANRKSVANKKKNATQTALSPKRPLSPAERKVILFNKPYDTLSQFTDGDGRKTLADYIPIKDVYAAGRLDRDSEGLL
ILTNDGILQARLTQPQSKAPKTYWVQVEGSPQESDLEALRHGVTLKDGPTLPAKVDIMPEPTLWPRNPPVRFRAAIPTTW
LAITLMEGRNRQVRRMTAHIGFPTLRLIRYSMGDWNLGDLQPGEWREVTL

Sequences:

>Translated_290_residues
MKVFIIKQIEDNSLLSCSLNLFQGANDSRSRLYGKLSNTVYLHFCKVLNMSSRLSSDATSSSSDQRKSHFKQRAAKNAGH
PPTRANRKSVANKKKNATQTALSPKRPLSPAERKVILFNKPYDTLSQFTDGDGRKTLADYIPIKDVYAAGRLDRDSEGLL
ILTNDGILQARLTQPQSKAPKTYWVQVEGSPQESDLEALRHGVTLKDGPTLPAKVDIMPEPTLWPRNPPVRFRAAIPTTW
LAITLMEGRNRQVRRMTAHIGFPTLRLIRYSMGDWNLGDLQPGEWREVTL
>Mature_290_residues
MKVFIIKQIEDNSLLSCSLNLFQGANDSRSRLYGKLSNTVYLHFCKVLNMSSRLSSDATSSSSDQRKSHFKQRAAKNAGH
PPTRANRKSVANKKKNATQTALSPKRPLSPAERKVILFNKPYDTLSQFTDGDGRKTLADYIPIKDVYAAGRLDRDSEGLL
ILTNDGILQARLTQPQSKAPKTYWVQVEGSPQESDLEALRHGVTLKDGPTLPAKVDIMPEPTLWPRNPPVRFRAAIPTTW
LAITLMEGRNRQVRRMTAHIGFPTLRLIRYSMGDWNLGDLQPGEWREVTL

Specific function: Responsible for synthesis of pseudouridine from uracil- 2457 in 23S ribosomal RNA [H]

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pseudouridine synthase rsuA family [H]

Homologues:

Organism=Escherichia coli, GI87081838, Length=177, Percent_Identity=71.1864406779661, Blast_Score=266, Evalue=1e-72,
Organism=Escherichia coli, GI1787524, Length=181, Percent_Identity=33.7016574585635, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1790453, Length=176, Percent_Identity=30.6818181818182, Blast_Score=79, Evalue=2e-16,
Organism=Escherichia coli, GI1788510, Length=147, Percent_Identity=34.0136054421769, Blast_Score=74, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496 [H]

Pfam domain/function: PF00849 PseudoU_synth_2 [H]

EC number: NA

Molecular weight: Translated: 32612; Mature: 32612

Theoretical pI: Translated: 10.65; Mature: 10.65

Prosite motif: PS01149 PSI_RSU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVFIIKQIEDNSLLSCSLNLFQGANDSRSRLYGKLSNTVYLHFCKVLNMSSRLSSDATS
CEEEEEEEECCCCEEEEEEHHHCCCCCHHHHHHHHHCCEEEEEHHHHHHHHHHHCCCCCC
SSSDQRKSHFKQRAAKNAGHPPTRANRKSVANKKKNATQTALSPKRPLSPAERKVILFNK
CCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCHHHHCCCCCCCCCCCCEEEEEEC
PYDTLSQFTDGDGRKTLADYIPIKDVYAAGRLDRDSEGLLILTNDGILQARLTQPQSKAP
CHHHHHHCCCCCCCHHHHHHCCHHHHHHHCCCCCCCCCEEEEECCCEEEEEECCCCCCCC
KTYWVQVEGSPQESDLEALRHGVTLKDGPTLPAKVDIMPEPTLWPRNPPVRFRAAIPTTW
CEEEEEEECCCCHHHHHHHHCCCEECCCCCCCEEEEECCCCCCCCCCCCEEEEEECCCEE
LAITLMEGRNRQVRRMTAHIGFPTLRLIRYSMGDWNLGDLQPGEWREVTL
EEEEEECCCCCHHHHHHHHCCCCHHHHHEECCCCCCCCCCCCCCEEECCC
>Mature Secondary Structure
MKVFIIKQIEDNSLLSCSLNLFQGANDSRSRLYGKLSNTVYLHFCKVLNMSSRLSSDATS
CEEEEEEEECCCCEEEEEEHHHCCCCCHHHHHHHHHCCEEEEEHHHHHHHHHHHCCCCCC
SSSDQRKSHFKQRAAKNAGHPPTRANRKSVANKKKNATQTALSPKRPLSPAERKVILFNK
CCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCHHHHCCCCCCCCCCCCEEEEEEC
PYDTLSQFTDGDGRKTLADYIPIKDVYAAGRLDRDSEGLLILTNDGILQARLTQPQSKAP
CHHHHHHCCCCCCCHHHHHHCCHHHHHHHCCCCCCCCCEEEEECCCEEEEEECCCCCCCC
KTYWVQVEGSPQESDLEALRHGVTLKDGPTLPAKVDIMPEPTLWPRNPPVRFRAAIPTTW
CEEEEEEECCCCHHHHHHHHCCCEECCCCCCCEEEEECCCCCCCCCCCCEEEEEECCCEE
LAITLMEGRNRQVRRMTAHIGFPTLRLIRYSMGDWNLGDLQPGEWREVTL
EEEEEECCCCCHHHHHHHHCCCCHHHHHEECCCCCCCCCCCCCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]