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The map label for this gene is ppx [H]

Identifier: 227080901

GI number: 227080901

Start: 732771

End: 734342

Strand: Reverse

Name: ppx [H]

Synonym: VCM66_0680

Alternate gene names: 227080901

Gene position: 734342-732771 (Counterclockwise)

Preceding gene: 227080902

Following gene: 227080897

Centisome position: 25.39

GC content: 47.39

Gene sequence:

>1572_bases
TTGCCATTTACGACTATCTTAAGAATGTCGAGAAGCAGACTCGTAAAGCAAAAGGGCAACAAGAAACGAATGACAACAGT
AGTCAGTAACGCAAGAGAAATCGCTGCCATTGATCTGGGTTCGAACAGTTTTCATATGGTGGTCGCCAAGGTGGTCGATC
AAGATCTCCAATTGATTAGCCGCCATAAGCAGCGTGTTCGTCTTGCTGCGGGCTTGGATGAACAAAAAAATCTCGATGAA
GAGTCGATCCAACGTGGCTTAGAGTGTTTGGCGATGTTCGCAGAACGATTGCAAGGCTTTGAGCCACGTAATGTCCGTAT
TGCTGCAACCCACACTCTGCGCCAAGCCAGAAATGCCAATCTGTTTATTCAACGCGCTCTCGACGTGTTGCCTTTTCCAA
TTGAAATCATTCCGGGCTCTGAAGAAGCCCGGTTGATTTATCTGGGGGTTGCCCATACCCAACCCCAAGCGGATTCTATG
CTGGTAGTGGATATTGGCGGCGGCAGCACGGAAATGATCATCGGCAAAGGCTTTGAAGCAGAACTGCTCAACAGTAAGCA
GATGGGCTGCGTCAACTTTACCGAGCGTTACTTTGCCAACGGCAAGCTATCACGTAAAAACTTTGCCCAAGCGATTGTCG
CCAGTGAGCAAAAATTAGAATCCATTGCCAGCAAATACCGTAAGAAAGGTTGGCAAATGGCGTTTGGTTCTTCCGGTACC
ATCAAAGCGATTCATGAAGTGTTAATCGGGCAAGGTCATGAAGATGGACTCATCACCTTTGAGCGCCTGAGCAAGTTAAT
TGAAAAACTCTGCGAATGGGACAGCATTGATGATTTACAACTGCCCGGCTTAACCGATGATCGAAAACCCGTGTTTGCGG
CGGGCGTTGCCATTCTTTCCGCGATCTTTCATGGTTTAAACATCAAAGAGATGCATTTTTCGGATGGCGCTTTGCGCGAA
GGTCTGCTGTATGAGATGGAAGATCGCTTCAAGTATTCCGATATCCGTTTGCGCACCACTGAAAATCTGGCCGCCAAACA
TCTGGTCGATTTAGAGCATGCCGCCAAAGTCAAAGGTCACGCGCGTGAATTCTTGGCGCAAGTCGCCAATGAGTTGGGAT
TACCAGAAGGCAGTGAGCTGTGCGATCTGCTCGAATGGGGCGCTTTACTACATGAAGTGGGGCTGAGCATTAACTTACAA
GGCTTTCATCGTCACTCCGCGTATATTTTGCGTCATAACAACATGGCAGGTTTCAACAGCGAACAACAACTCGTGCTCTC
CAATTTGGCACGTTTCCAACGTAAATCGCTGAAATTGAATGAGCTGGATGACTTTAGTCTGTTTAAGAAAAAACACATTA
TTGGCTTAATTCGAGTCCTACGACTGGCGATTGTGGTTAATGGGCAACGCAACGACGACCCTTTACCACCATTGACCCTA
TCCGCCAAAGATGATGAGTGGCGATTGGAGTGTGAACAGCCAGATTGGCTGGAAAACAATAAGTTGTTGCATGCTGACTT
ACAAACAGAGCAAGAATACTGGCGTGAAGTCGGCTGGCAACTGCTGTTTTAA

Upstream 100 bases:

>100_bases
AATATTCAGTTCATTGATACGGTCAAAGCGCGTCGCATCGACAAAGAGATGAGCAATCAGTACGTAGAGCGCGGTAATCG
CAGAAAAGTACGCTCTCAGA

Downstream 100 bases:

>100_bases
GCCAGACTAGTCACTGCAATTCGATGAAAAACGCCCAACTCAAGTTGGGCGTTTTTCATTTCATCACTCAGTCGATGATG
GGCTTAATGCCAACTTTGGC

Product: exopolyphosphatase

Products: NA

Alternate protein names: ExopolyPase; Metaphosphatase [H]

Number of amino acids: Translated: 523; Mature: 522

Protein sequence:

>523_residues
MPFTTILRMSRSRLVKQKGNKKRMTTVVSNAREIAAIDLGSNSFHMVVAKVVDQDLQLISRHKQRVRLAAGLDEQKNLDE
ESIQRGLECLAMFAERLQGFEPRNVRIAATHTLRQARNANLFIQRALDVLPFPIEIIPGSEEARLIYLGVAHTQPQADSM
LVVDIGGGSTEMIIGKGFEAELLNSKQMGCVNFTERYFANGKLSRKNFAQAIVASEQKLESIASKYRKKGWQMAFGSSGT
IKAIHEVLIGQGHEDGLITFERLSKLIEKLCEWDSIDDLQLPGLTDDRKPVFAAGVAILSAIFHGLNIKEMHFSDGALRE
GLLYEMEDRFKYSDIRLRTTENLAAKHLVDLEHAAKVKGHAREFLAQVANELGLPEGSELCDLLEWGALLHEVGLSINLQ
GFHRHSAYILRHNNMAGFNSEQQLVLSNLARFQRKSLKLNELDDFSLFKKKHIIGLIRVLRLAIVVNGQRNDDPLPPLTL
SAKDDEWRLECEQPDWLENNKLLHADLQTEQEYWREVGWQLLF

Sequences:

>Translated_523_residues
MPFTTILRMSRSRLVKQKGNKKRMTTVVSNAREIAAIDLGSNSFHMVVAKVVDQDLQLISRHKQRVRLAAGLDEQKNLDE
ESIQRGLECLAMFAERLQGFEPRNVRIAATHTLRQARNANLFIQRALDVLPFPIEIIPGSEEARLIYLGVAHTQPQADSM
LVVDIGGGSTEMIIGKGFEAELLNSKQMGCVNFTERYFANGKLSRKNFAQAIVASEQKLESIASKYRKKGWQMAFGSSGT
IKAIHEVLIGQGHEDGLITFERLSKLIEKLCEWDSIDDLQLPGLTDDRKPVFAAGVAILSAIFHGLNIKEMHFSDGALRE
GLLYEMEDRFKYSDIRLRTTENLAAKHLVDLEHAAKVKGHAREFLAQVANELGLPEGSELCDLLEWGALLHEVGLSINLQ
GFHRHSAYILRHNNMAGFNSEQQLVLSNLARFQRKSLKLNELDDFSLFKKKHIIGLIRVLRLAIVVNGQRNDDPLPPLTL
SAKDDEWRLECEQPDWLENNKLLHADLQTEQEYWREVGWQLLF
>Mature_522_residues
PFTTILRMSRSRLVKQKGNKKRMTTVVSNAREIAAIDLGSNSFHMVVAKVVDQDLQLISRHKQRVRLAAGLDEQKNLDEE
SIQRGLECLAMFAERLQGFEPRNVRIAATHTLRQARNANLFIQRALDVLPFPIEIIPGSEEARLIYLGVAHTQPQADSML
VVDIGGGSTEMIIGKGFEAELLNSKQMGCVNFTERYFANGKLSRKNFAQAIVASEQKLESIASKYRKKGWQMAFGSSGTI
KAIHEVLIGQGHEDGLITFERLSKLIEKLCEWDSIDDLQLPGLTDDRKPVFAAGVAILSAIFHGLNIKEMHFSDGALREG
LLYEMEDRFKYSDIRLRTTENLAAKHLVDLEHAAKVKGHAREFLAQVANELGLPEGSELCDLLEWGALLHEVGLSINLQG
FHRHSAYILRHNNMAGFNSEQQLVLSNLARFQRKSLKLNELDDFSLFKKKHIIGLIRVLRLAIVVNGQRNDDPLPPLTLS
AKDDEWRLECEQPDWLENNKLLHADLQTEQEYWREVGWQLLF

Specific function: Degradation of inorganic polyphosphates. Orthophosphate is released progressively from the ends of polyphosphate [H]

COG id: COG0248

COG function: function code FP; Exopolyphosphatase

Gene ontology:

Cell location: Membrane-Associated [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gppA/ppx family [H]

Homologues:

Organism=Escherichia coli, GI1788848, Length=492, Percent_Identity=50.8130081300813, Blast_Score=495, Evalue=1e-141,
Organism=Escherichia coli, GI48994966, Length=493, Percent_Identity=41.1764705882353, Blast_Score=355, Evalue=5e-99,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022371
- InterPro:   IPR003695 [H]

Pfam domain/function: PF02541 Ppx-GppA [H]

EC number: =3.6.1.11 [H]

Molecular weight: Translated: 59204; Mature: 59073

Theoretical pI: Translated: 7.13; Mature: 7.13

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPFTTILRMSRSRLVKQKGNKKRMTTVVSNAREIAAIDLGSNSFHMVVAKVVDQDLQLIS
CCHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCEEEEEEECCCCHHHHHHHHHHHHHHHHH
RHKQRVRLAAGLDEQKNLDEESIQRGLECLAMFAERLQGFEPRNVRIAATHTLRQARNAN
HHHHHHHHHCCCCHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEHHHHHHHCCCC
LFIQRALDVLPFPIEIIPGSEEARLIYLGVAHTQPQADSMLVVDIGGGSTEMIIGKGFEA
HHHHHHHHHCCCCEEEECCCCCCEEEEEEEECCCCCCCCEEEEEECCCCEEEEEECCCCH
ELLNSKQMGCVNFTERYFANGKLSRKNFAQAIVASEQKLESIASKYRKKGWQMAFGSSGT
HHCCCCCCCCCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCH
IKAIHEVLIGQGHEDGLITFERLSKLIEKLCEWDSIDDLQLPGLTDDRKPVFAAGVAILS
HHHHHHHHHCCCCCCCCEEHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHH
AIFHGLNIKEMHFSDGALREGLLYEMEDRFKYSDIRLRTTENLAAKHLVDLEHAAKVKGH
HHHHCCCEEEEECCCCHHHCCCHHHHHHCCEECCEEEEECHHHHHHHHHHHHHHHHHHHH
AREFLAQVANELGLPEGSELCDLLEWGALLHEVGLSINLQGFHRHSAYILRHNNMAGFNS
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCEEEEEEEECCCEEEEEECCCCCCCC
EQQLVLSNLARFQRKSLKLNELDDFSLFKKKHIIGLIRVLRLAIVVNGQRNDDPLPPLTL
HHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEE
SAKDDEWRLECEQPDWLENNKLLHADLQTEQEYWREVGWQLLF
ECCCCCEEEECCCCCCCCCCCEEEECCHHHHHHHHHHCCEECC
>Mature Secondary Structure 
PFTTILRMSRSRLVKQKGNKKRMTTVVSNAREIAAIDLGSNSFHMVVAKVVDQDLQLIS
CHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCEEEEEEECCCCHHHHHHHHHHHHHHHHH
RHKQRVRLAAGLDEQKNLDEESIQRGLECLAMFAERLQGFEPRNVRIAATHTLRQARNAN
HHHHHHHHHCCCCHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEHHHHHHHCCCC
LFIQRALDVLPFPIEIIPGSEEARLIYLGVAHTQPQADSMLVVDIGGGSTEMIIGKGFEA
HHHHHHHHHCCCCEEEECCCCCCEEEEEEEECCCCCCCCEEEEEECCCCEEEEEECCCCH
ELLNSKQMGCVNFTERYFANGKLSRKNFAQAIVASEQKLESIASKYRKKGWQMAFGSSGT
HHCCCCCCCCCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCH
IKAIHEVLIGQGHEDGLITFERLSKLIEKLCEWDSIDDLQLPGLTDDRKPVFAAGVAILS
HHHHHHHHHCCCCCCCCEEHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHH
AIFHGLNIKEMHFSDGALREGLLYEMEDRFKYSDIRLRTTENLAAKHLVDLEHAAKVKGH
HHHHCCCEEEEECCCCHHHCCCHHHHHHCCEECCEEEEECHHHHHHHHHHHHHHHHHHHH
AREFLAQVANELGLPEGSELCDLLEWGALLHEVGLSINLQGFHRHSAYILRHNNMAGFNS
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCEEEEEEEECCCEEEEEECCCCCCCC
EQQLVLSNLARFQRKSLKLNELDDFSLFKKKHIIGLIRVLRLAIVVNGQRNDDPLPPLTL
HHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEE
SAKDDEWRLECEQPDWLENNKLLHADLQTEQEYWREVGWQLLF
ECCCCCEEEECCCCCCCCCCCEEEECCHHHHHHHHHHCCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]