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The map label for this gene is ppk

Identifier: 227080902

GI number: 227080902

Start: 734254

End: 736359

Strand: Reverse

Name: ppk

Synonym: VCM66_0681

Alternate gene names: 227080902

Gene position: 736359-734254 (Counterclockwise)

Preceding gene: 227080907

Following gene: 227080901

Centisome position: 25.46

GC content: 46.87

Gene sequence:

>2106_bases
ATGAGTGCGGATAAGCTGTACATCGACAAGGAACTCAGTTGGCTCTCATTTAACGAGCGCGTATTACAAGAAGCGGCAGA
TAAAACCGTCCCGCTGATTGAGCGGATTCGTTTTCTGGGGATATTTTCCAATAACCTCGATGAGTTTTATAAGGTGAGAT
TTGCTGACGTAAAACGACAAATCTTGATCAATCGAGAACGGGGGGGCAATGATATCTCCAAACACCTCCTCTCACGCATG
CAAAGCAAAGCCCTCAAACTCAACCAAGACTTCGATAACCTCTACAACGAATTGATTTTAGAAATGGCCAGACGGCGCAT
TTTCTTAGTCAACGAAACGCAACTCGATGAAATCCAACTCAAGTGGGTGAAAAAGTATTTCCACAAAGAAGTGCTGCCGC
ATGTCACCCCTATCATGCTGCGTGATGACATTGATGTCATGCAGTTTTTAAAAGACGAATACGCTTACATTGCGGTCGAA
ATGCGCAGTGGCGATGAATTTAAATACGCGCTGATCGAGATTCCAACCGACCAACTGCCACGCTTTGTGATGCTGCCTGA
GCAAAAAGGCAAACGGCGCAAAACCATTATTCTGCTCGATAACATTATTCGTCTCTGTCTGGATGAGATTTTCCGCGGTT
TTTACGACTACGATACACTCAACGGTTACGCGATGAAAATGACCCGCGATGCCGAGTATGATTTGCGCCACGAAGTCGAA
TACAGCTTGCTTGAGCAGATGTCAGAAGGCTTAAGCCAGCGCTTAACCGCCCTTCCTGTGCGCTTTGTGTATGAGCGTGA
AATGCCTGAAGCCATGCTCAAATTCTTATGCTATAAGCTGAAAATTTCCCACTATGACAGCTTGATCCCCGGTGGACGTT
ACCACAATTTTAAAGACTTTATTTCTTTCCCCAATGTCGGCCGAGATTATTTAGAAAACAAACCCTTGCCTCCGATGACT
TGTGCTGACTTTGAAGGCTATGCCAATGCTTTTGATGCGATTCGCGCGCAAGATATTTTACTGCACTACCCTTATCACAG
CTTTGAGCACATGACGGAGCTTGTGCGCCAAGCCTCTTTTGATCCGAAAGTCGTCAGTATCAAAATCAACATTTACCGCG
TAGCCAAAGACTCCAAATTGATGAACTCGCTGGTCGATGCGGTGCACAACGGCAAACGTGTGGTGGTGGTGGTTGAGCTG
CAAGCTCGTTTTGATGAAGAGGCCAATATCGAGTGGTCACGCATTCTGACCGATGCGGGCGTACACGTTATTTTCGGTGT
GCCGGGAATGAAGATTCACGCCAAGCTCCTGCTTATTACCCGTAAAGAAGGGGATGAATTCGTTCGTTACGCGCACATTG
GTACCGGCAACTTCCACGAACGTACGGCGCGCATTTACACCGACTTTGCCTTGCTCACCGCCAACCAAGAGCTCGCCGCC
GAAGTCCGTGCCGTATTTGGTTACATCGAAAATCCATTCCGCCCCGTCAAGTTTAACCACCTGATCGTATCGCCGCGTAA
CTCACGTACCCAAATTTATCGTTTACTGGATAGTGAAATCGCCAACGCCAAGGCGGGCAAAAAAGCCGCCATCACCCTTA
AAGTGAACAATCTCGTTGATAAGGGATTGATCAATAAGCTGTATGGTGCAAGTGCCGCTGGAGTCAAAATCCGCATGATC
ATCCGCGGTATGTGCTCTTTAGTCCCCGGAGTGGAAGGTGTCAGTGACAATATTGAGATCATCAGTATTATTGACCGCTT
CCTCGAACACCCTCGCGTGTTAGTGGTACATAATGATGGCAATCCACAGGTCTTTATCTCATCGGCAGACTGGATGGAGC
GCAATATAGATCACCGAATTGAGGTCATGGCTCCAATTCGTGACGAACGACTCAAACAGCGTATCATTGATATCCTTAAT
ATTCAGTTCATTGATACGGTCAAAGCGCGTCGCATCGACAAAGAGATGAGCAATCAGTACGTAGAGCGCGGTAATCGCAG
AAAAGTACGCTCTCAGATTGCCATTTACGACTATCTTAAGAATGTCGAGAAGCAGACTCGTAAAGCAAAAGGGCAACAAG
AAACGAATGACAACAGTAGTCAGTAA

Upstream 100 bases:

>100_bases
TATGACACTAAAGGCACTGCAATAAAAGTGTCACAAAAATCACTTAGTTTGCAGCTCTTTAATGATGAAAACGTCAACTC
AAAACGGCATAAGGTAAAGT

Downstream 100 bases:

>100_bases
CGCAAGAGAAATCGCTGCCATTGATCTGGGTTCGAACAGTTTTCATATGGTGGTCGCCAAGGTGGTCGATCAAGATCTCC
AATTGATTAGCCGCCATAAG

Product: polyphosphate kinase

Products: NA

Alternate protein names: ATP-polyphosphate phosphotransferase; Polyphosphoric acid kinase

Number of amino acids: Translated: 701; Mature: 700

Protein sequence:

>701_residues
MSADKLYIDKELSWLSFNERVLQEAADKTVPLIERIRFLGIFSNNLDEFYKVRFADVKRQILINRERGGNDISKHLLSRM
QSKALKLNQDFDNLYNELILEMARRRIFLVNETQLDEIQLKWVKKYFHKEVLPHVTPIMLRDDIDVMQFLKDEYAYIAVE
MRSGDEFKYALIEIPTDQLPRFVMLPEQKGKRRKTIILLDNIIRLCLDEIFRGFYDYDTLNGYAMKMTRDAEYDLRHEVE
YSLLEQMSEGLSQRLTALPVRFVYEREMPEAMLKFLCYKLKISHYDSLIPGGRYHNFKDFISFPNVGRDYLENKPLPPMT
CADFEGYANAFDAIRAQDILLHYPYHSFEHMTELVRQASFDPKVVSIKINIYRVAKDSKLMNSLVDAVHNGKRVVVVVEL
QARFDEEANIEWSRILTDAGVHVIFGVPGMKIHAKLLLITRKEGDEFVRYAHIGTGNFHERTARIYTDFALLTANQELAA
EVRAVFGYIENPFRPVKFNHLIVSPRNSRTQIYRLLDSEIANAKAGKKAAITLKVNNLVDKGLINKLYGASAAGVKIRMI
IRGMCSLVPGVEGVSDNIEIISIIDRFLEHPRVLVVHNDGNPQVFISSADWMERNIDHRIEVMAPIRDERLKQRIIDILN
IQFIDTVKARRIDKEMSNQYVERGNRRKVRSQIAIYDYLKNVEKQTRKAKGQQETNDNSSQ

Sequences:

>Translated_701_residues
MSADKLYIDKELSWLSFNERVLQEAADKTVPLIERIRFLGIFSNNLDEFYKVRFADVKRQILINRERGGNDISKHLLSRM
QSKALKLNQDFDNLYNELILEMARRRIFLVNETQLDEIQLKWVKKYFHKEVLPHVTPIMLRDDIDVMQFLKDEYAYIAVE
MRSGDEFKYALIEIPTDQLPRFVMLPEQKGKRRKTIILLDNIIRLCLDEIFRGFYDYDTLNGYAMKMTRDAEYDLRHEVE
YSLLEQMSEGLSQRLTALPVRFVYEREMPEAMLKFLCYKLKISHYDSLIPGGRYHNFKDFISFPNVGRDYLENKPLPPMT
CADFEGYANAFDAIRAQDILLHYPYHSFEHMTELVRQASFDPKVVSIKINIYRVAKDSKLMNSLVDAVHNGKRVVVVVEL
QARFDEEANIEWSRILTDAGVHVIFGVPGMKIHAKLLLITRKEGDEFVRYAHIGTGNFHERTARIYTDFALLTANQELAA
EVRAVFGYIENPFRPVKFNHLIVSPRNSRTQIYRLLDSEIANAKAGKKAAITLKVNNLVDKGLINKLYGASAAGVKIRMI
IRGMCSLVPGVEGVSDNIEIISIIDRFLEHPRVLVVHNDGNPQVFISSADWMERNIDHRIEVMAPIRDERLKQRIIDILN
IQFIDTVKARRIDKEMSNQYVERGNRRKVRSQIAIYDYLKNVEKQTRKAKGQQETNDNSSQ
>Mature_700_residues
SADKLYIDKELSWLSFNERVLQEAADKTVPLIERIRFLGIFSNNLDEFYKVRFADVKRQILINRERGGNDISKHLLSRMQ
SKALKLNQDFDNLYNELILEMARRRIFLVNETQLDEIQLKWVKKYFHKEVLPHVTPIMLRDDIDVMQFLKDEYAYIAVEM
RSGDEFKYALIEIPTDQLPRFVMLPEQKGKRRKTIILLDNIIRLCLDEIFRGFYDYDTLNGYAMKMTRDAEYDLRHEVEY
SLLEQMSEGLSQRLTALPVRFVYEREMPEAMLKFLCYKLKISHYDSLIPGGRYHNFKDFISFPNVGRDYLENKPLPPMTC
ADFEGYANAFDAIRAQDILLHYPYHSFEHMTELVRQASFDPKVVSIKINIYRVAKDSKLMNSLVDAVHNGKRVVVVVELQ
ARFDEEANIEWSRILTDAGVHVIFGVPGMKIHAKLLLITRKEGDEFVRYAHIGTGNFHERTARIYTDFALLTANQELAAE
VRAVFGYIENPFRPVKFNHLIVSPRNSRTQIYRLLDSEIANAKAGKKAAITLKVNNLVDKGLINKLYGASAAGVKIRMII
RGMCSLVPGVEGVSDNIEIISIIDRFLEHPRVLVVHNDGNPQVFISSADWMERNIDHRIEVMAPIRDERLKQRIIDILNI
QFIDTVKARRIDKEMSNQYVERGNRRKVRSQIAIYDYLKNVEKQTRKAKGQQETNDNSSQ

Specific function: Catalyzes the reversible transfer of the terminal phosphate of ATP to form a long-chain polyphosphate (polyP)

COG id: COG0855

COG function: function code P; Polyphosphate kinase

Gene ontology:

Cell location: Membrane [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 PLD phosphodiesterase domains

Homologues:

Organism=Escherichia coli, GI1788847, Length=686, Percent_Identity=64.1399416909621, Blast_Score=947, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PPK_VIBCH (Q9KU07)

Other databases:

- EMBL:   AF083928
- EMBL:   AE003852
- PIR:   D82289
- RefSeq:   NP_230372.1
- ProteinModelPortal:   Q9KU07
- SMR:   Q9KU07
- GeneID:   2615732
- GenomeReviews:   AE003852_GR
- KEGG:   vch:VC0723
- TIGR:   VC_0723
- HOGENOM:   HBG341268
- OMA:   FLISRRE
- ProtClustDB:   PRK05443
- BRENDA:   2.7.4.1
- HAMAP:   MF_00347
- InterPro:   IPR001736
- InterPro:   IPR003414
- PIRSF:   PIRSF015589
- TIGRFAMs:   TIGR03705

Pfam domain/function: PF02503 PP_kinase

EC number: =2.7.4.1

Molecular weight: Translated: 81646; Mature: 81515

Theoretical pI: Translated: 8.93; Mature: 8.93

Prosite motif: PS50035 PLD

Important sites: ACT_SITE 433-433 ACT_SITE 452-452

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSADKLYIDKELSWLSFNERVLQEAADKTVPLIERIRFLGIFSNNLDEFYKVRFADVKRQ
CCCCCEEEECCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
ILINRERGGNDISKHLLSRMQSKALKLNQDFDNLYNELILEMARRRIFLVNETQLDEIQL
HEEECCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCEEEEEECCCHHHHHH
KWVKKYFHKEVLPHVTPIMLRDDIDVMQFLKDEYAYIAVEMRSGDEFKYALIEIPTDQLP
HHHHHHHHHHHHHCCCCEEEECCHHHHHHHHCCCEEEEEEECCCCCEEEEEEECCHHHCC
RFVMLPEQKGKRRKTIILLDNIIRLCLDEIFRGFYDYDTLNGYAMKMTRDAEYDLRHEVE
CEEECCCCCCCCCCEEEEHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCHHHHHH
YSLLEQMSEGLSQRLTALPVRFVYEREMPEAMLKFLCYKLKISHYDSLIPGGRYHNFKDF
HHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHH
ISFPNVGRDYLENKPLPPMTCADFEGYANAFDAIRAQDILLHYPYHSFEHMTELVRQASF
HCCCCCCHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHEEEECCCHHHHHHHHHHHHCCC
DPKVVSIKINIYRVAKDSKLMNSLVDAVHNGKRVVVVVELQARFDEEANIEWSRILTDAG
CCEEEEEEEEEEEECCHHHHHHHHHHHHHCCCEEEEEEEECCCCCCCCCCCHHHHHHHCC
VHVIFGVPGMKIHAKLLLITRKEGDEFVRYAHIGTGNFHERTARIYTDFALLTANQELAA
CEEEECCCCCEEEEEEEEEEECCCCCEEEEEEECCCCHHHHHHHHHEEEEEEECCHHHHH
EVRAVFGYIENPFRPVKFNHLIVSPRNSRTQIYRLLDSEIANAKAGKKAAITLKVNNLVD
HHHHHHHHHCCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHCCCCCCEEEEEEEEHHHHH
KGLINKLYGASAAGVKIRMIIRGMCSLVPGVEGVSDNIEIISIIDRFLEHPRVLVVHNDG
HHHHHHHHCCCCCCEEHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCEEEEEEECC
NPQVFISSADWMERNIDHRIEVMAPIRDERLKQRIIDILNIQFIDTVKARRIDKEMSNQY
CCEEEEECCHHHHCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VERGNRRKVRSQIAIYDYLKNVEKQTRKAKGQQETNDNSSQ
HHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure 
SADKLYIDKELSWLSFNERVLQEAADKTVPLIERIRFLGIFSNNLDEFYKVRFADVKRQ
CCCCEEEECCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
ILINRERGGNDISKHLLSRMQSKALKLNQDFDNLYNELILEMARRRIFLVNETQLDEIQL
HEEECCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCEEEEEECCCHHHHHH
KWVKKYFHKEVLPHVTPIMLRDDIDVMQFLKDEYAYIAVEMRSGDEFKYALIEIPTDQLP
HHHHHHHHHHHHHCCCCEEEECCHHHHHHHHCCCEEEEEEECCCCCEEEEEEECCHHHCC
RFVMLPEQKGKRRKTIILLDNIIRLCLDEIFRGFYDYDTLNGYAMKMTRDAEYDLRHEVE
CEEECCCCCCCCCCEEEEHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCHHHHHH
YSLLEQMSEGLSQRLTALPVRFVYEREMPEAMLKFLCYKLKISHYDSLIPGGRYHNFKDF
HHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHH
ISFPNVGRDYLENKPLPPMTCADFEGYANAFDAIRAQDILLHYPYHSFEHMTELVRQASF
HCCCCCCHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHEEEECCCHHHHHHHHHHHHCCC
DPKVVSIKINIYRVAKDSKLMNSLVDAVHNGKRVVVVVELQARFDEEANIEWSRILTDAG
CCEEEEEEEEEEEECCHHHHHHHHHHHHHCCCEEEEEEEECCCCCCCCCCCHHHHHHHCC
VHVIFGVPGMKIHAKLLLITRKEGDEFVRYAHIGTGNFHERTARIYTDFALLTANQELAA
CEEEECCCCCEEEEEEEEEEECCCCCEEEEEEECCCCHHHHHHHHHEEEEEEECCHHHHH
EVRAVFGYIENPFRPVKFNHLIVSPRNSRTQIYRLLDSEIANAKAGKKAAITLKVNNLVD
HHHHHHHHHCCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHCCCCCCEEEEEEEEHHHHH
KGLINKLYGASAAGVKIRMIIRGMCSLVPGVEGVSDNIEIISIIDRFLEHPRVLVVHNDG
HHHHHHHHCCCCCCEEHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCEEEEEEECC
NPQVFISSADWMERNIDHRIEVMAPIRDERLKQRIIDILNIQFIDTVKARRIDKEMSNQY
CCEEEEECCHHHHCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VERGNRRKVRSQIAIYDYLKNVEKQTRKAKGQQETNDNSSQ
HHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 10952301