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The map label for this gene is recA [H]

Identifier: 227080726

GI number: 227080726

Start: 531969

End: 533207

Strand: Direct

Name: recA [H]

Synonym: VCM66_0501

Alternate gene names: 227080726

Gene position: 531969-533207 (Clockwise)

Preceding gene: 227080725

Following gene: 227080727

Centisome position: 18.39

GC content: 47.13

Gene sequence:

>1239_bases
TTGAGCATCTCGCAGCAGATCAGGTTCAAAACGTTAACTGAGCCAAACTTTTTTCTATACAGGGGTGGACACTGTACAAA
TCAACAGTATAATGAACCCCAACATGACAAATCAGCGTCGACCCGATTTCGACACAATTACCTATCACCTGAGAGTGACT
ATCCGGAGAGAGTAATGGACGAGAATAAACAGAAGGCACTTGCGGCCGCCCTCGGTCAAATTGAAAAGCAATTTGGTAAA
GGCTCCATCATGCGCTTAGGCGACAACCGCGCGATGGATGTAGAAACCATTTCGACCGGTTCTCTGTCTCTGGATATCGC
GTTGGGTGCTGGTGGTCTACCAATGGGACGTATCGTTGAGATCTTCGGCCCTGAATCTTCGGGTAAAACGACACTGACTC
TGGAACTGATTGCTGCTGCACAACGTGAAGGTAAAACCTGTGCGTTTATCGATGCCGAGCACGCACTGGATCCGGTTTAT
GCCAAAAAATTGGGCGTGAATATCGATGAGCTACTGGTTTCTCAGCCAGATACCGGCGAGCAAGCACTGGAAATTTGTGA
TGCACTGGCTCGCTCTGGTGCTGTGGATGTCATCGTTGTTGACTCAGTAGCGGCTTTGACACCAAAAGCGGAAATTGAAG
GCGAAATGGGCGATAGCCACATGGGTCTGCAAGCGCGTATGTTGTCGCAAGCAATGCGTAAACTGACGGGTAACCTCAAG
CAATCCAACTGTATGTGTATCTTCATCAACCAAATTCGTATGAAGATTGGTGTGATGTTTGGTAACCCAGAAACTACCAC
TGGCGGTAACGCACTGAAATTCTACGCTTCTGTTCGTTTGGATATTCGCCGTACTGGCGCAATCAAAGAAGGCGAAGAAG
TGGTGGGTAACGAAACCCGTATCAAGGTTGTGAAGAACAAGATTGCTGCGCCGTTTAAAGAAGCGAACACTCAGATCATG
TATGGCCAAGGTTTCAATCGTGAAGGTGAATTGATCGATCTTGGTGTGAAACACAAAATGGTTGAGAAGTCAGGCGCTTG
GTACAGCTATAACGGCGATAAAATCGGCCAAGGTAAAGCGAATGCCTGCAAGTATCTGAAAGAAAACCCAGAAATTGCGA
AAACTCTAGATAAAAAACTGCGTGAAATGCTGTTAAACCCAGAAAACATGCAGCTGATCGCTGAAACATCTTCTGCGGCG
GATGACGTCGAATTTGGCGCGGTGCCAGAAGAGTTTTAA

Upstream 100 bases:

>100_bases
GCTAGTGTTCAAGGTTGGCTCAAAGTGGAAACGTGCCACTTTACGGGTGATCGCGAACAAGTGCGTCAGCAAGCCATAGC
GCATGTGTTGCAAAGCTTGA

Downstream 100 bases:

>100_bases
TCGGCAGGCTGAATGCAAAGAGGTTCAGCCTCTCAATCTCGATGTTGATGAAGCCCTGCAATGCAGGGCTTCTTTTTGGC
TGCCAATTGAGTGAGTGACT

Product: recombinase A

Products: NA

Alternate protein names: Recombinase A [H]

Number of amino acids: Translated: 412; Mature: 411

Protein sequence:

>412_residues
MSISQQIRFKTLTEPNFFLYRGGHCTNQQYNEPQHDKSASTRFRHNYLSPESDYPERVMDENKQKALAAALGQIEKQFGK
GSIMRLGDNRAMDVETISTGSLSLDIALGAGGLPMGRIVEIFGPESSGKTTLTLELIAAAQREGKTCAFIDAEHALDPVY
AKKLGVNIDELLVSQPDTGEQALEICDALARSGAVDVIVVDSVAALTPKAEIEGEMGDSHMGLQARMLSQAMRKLTGNLK
QSNCMCIFINQIRMKIGVMFGNPETTTGGNALKFYASVRLDIRRTGAIKEGEEVVGNETRIKVVKNKIAAPFKEANTQIM
YGQGFNREGELIDLGVKHKMVEKSGAWYSYNGDKIGQGKANACKYLKENPEIAKTLDKKLREMLLNPENMQLIAETSSAA
DDVEFGAVPEEF

Sequences:

>Translated_412_residues
MSISQQIRFKTLTEPNFFLYRGGHCTNQQYNEPQHDKSASTRFRHNYLSPESDYPERVMDENKQKALAAALGQIEKQFGK
GSIMRLGDNRAMDVETISTGSLSLDIALGAGGLPMGRIVEIFGPESSGKTTLTLELIAAAQREGKTCAFIDAEHALDPVY
AKKLGVNIDELLVSQPDTGEQALEICDALARSGAVDVIVVDSVAALTPKAEIEGEMGDSHMGLQARMLSQAMRKLTGNLK
QSNCMCIFINQIRMKIGVMFGNPETTTGGNALKFYASVRLDIRRTGAIKEGEEVVGNETRIKVVKNKIAAPFKEANTQIM
YGQGFNREGELIDLGVKHKMVEKSGAWYSYNGDKIGQGKANACKYLKENPEIAKTLDKKLREMLLNPENMQLIAETSSAA
DDVEFGAVPEEF
>Mature_411_residues
SISQQIRFKTLTEPNFFLYRGGHCTNQQYNEPQHDKSASTRFRHNYLSPESDYPERVMDENKQKALAAALGQIEKQFGKG
SIMRLGDNRAMDVETISTGSLSLDIALGAGGLPMGRIVEIFGPESSGKTTLTLELIAAAQREGKTCAFIDAEHALDPVYA
KKLGVNIDELLVSQPDTGEQALEICDALARSGAVDVIVVDSVAALTPKAEIEGEMGDSHMGLQARMLSQAMRKLTGNLKQ
SNCMCIFINQIRMKIGVMFGNPETTTGGNALKFYASVRLDIRRTGAIKEGEEVVGNETRIKVVKNKIAAPFKEANTQIMY
GQGFNREGELIDLGVKHKMVEKSGAWYSYNGDKIGQGKANACKYLKENPEIAKTLDKKLREMLLNPENMQLIAETSSAAD
DVEFGAVPEEF

Specific function: Can catalyze the hydrolysis of ATP in the presence of single-stranded DNA, the ATP-dependent uptake of single-stranded DNA by duplex DNA, and the ATP-dependent hybridization of homologous single-stranded DNAs. It interacts with lexA causing its activation

COG id: COG0468

COG function: function code L; RecA/RadA recombinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the recA family [H]

Homologues:

Organism=Escherichia coli, GI1789051, Length=331, Percent_Identity=84.2900302114804, Blast_Score=581, Evalue=1e-167,

Paralogues:

None

Copy number: 1548 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 800-1200 (L-broth) 40,000-60,000 (L-broth + Nalidixate) 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR013765
- InterPro:   IPR020584
- InterPro:   IPR020588
- InterPro:   IPR020587 [H]

Pfam domain/function: PF00154 RecA [H]

EC number: NA

Molecular weight: Translated: 45184; Mature: 45053

Theoretical pI: Translated: 5.78; Mature: 5.78

Prosite motif: PS00321 RECA_1 ; PS50162 RECA_2 ; PS50163 RECA_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSISQQIRFKTLTEPNFFLYRGGHCTNQQYNEPQHDKSASTRFRHNYLSPESDYPERVMD
CCCCCCCEEEEECCCCEEEEECCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCHHHHC
ENKQKALAAALGQIEKQFGKGSIMRLGDNRAMDVETISTGSLSLDIALGAGGLPMGRIVE
CHHHHHHHHHHHHHHHHHCCCCEEEECCCCCEEEEEECCCCEEEEEEECCCCCCHHHEEE
IFGPESSGKTTLTLELIAAAQREGKTCAFIDAEHALDPVYAKKLGVNIDELLVSQPDTGE
EECCCCCCCEEEEEEEEHHHHCCCCEEEEEEHHHCCCHHHHHHHCCCHHHHHCCCCCCHH
QALEICDALARSGAVDVIVVDSVAALTPKAEIEGEMGDSHMGLQARMLSQAMRKLTGNLK
HHHHHHHHHHHCCCEEEEEECCHHHHCCCHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCC
QSNCMCIFINQIRMKIGVMFGNPETTTGGNALKFYASVRLDIRRTGAIKEGEEVVGNETR
CCCEEEEEEEEEEEEEEEEECCCCCCCCCCEEEEEEEEEEEEEECCCCCCCHHHHCCCCE
IKVVKNKIAAPFKEANTQIMYGQGFNREGELIDLGVKHKMVEKSGAWYSYNGDKIGQGKA
EEEEHHHHCCCHHHCCCEEEECCCCCCCCCEEEECCHHHHHHCCCCEEEECCCCCCCCCH
NACKYLKENPEIAKTLDKKLREMLLNPENMQLIAETSSAADDVEFGAVPEEF
HHHHHHHCCCHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SISQQIRFKTLTEPNFFLYRGGHCTNQQYNEPQHDKSASTRFRHNYLSPESDYPERVMD
CCCCCCEEEEECCCCEEEEECCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCHHHHC
ENKQKALAAALGQIEKQFGKGSIMRLGDNRAMDVETISTGSLSLDIALGAGGLPMGRIVE
CHHHHHHHHHHHHHHHHHCCCCEEEECCCCCEEEEEECCCCEEEEEEECCCCCCHHHEEE
IFGPESSGKTTLTLELIAAAQREGKTCAFIDAEHALDPVYAKKLGVNIDELLVSQPDTGE
EECCCCCCCEEEEEEEEHHHHCCCCEEEEEEHHHCCCHHHHHHHCCCHHHHHCCCCCCHH
QALEICDALARSGAVDVIVVDSVAALTPKAEIEGEMGDSHMGLQARMLSQAMRKLTGNLK
HHHHHHHHHHHCCCEEEEEECCHHHHCCCHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCC
QSNCMCIFINQIRMKIGVMFGNPETTTGGNALKFYASVRLDIRRTGAIKEGEEVVGNETR
CCCEEEEEEEEEEEEEEEEECCCCCCCCCCEEEEEEEEEEEEEECCCCCCCHHHHCCCCE
IKVVKNKIAAPFKEANTQIMYGQGFNREGELIDLGVKHKMVEKSGAWYSYNGDKIGQGKA
EEEEHHHHCCCHHHCCCEEEECCCCCCCCCEEEECCHHHHHHCCCCEEEECCCCCCCCCH
NACKYLKENPEIAKTLDKKLREMLLNPENMQLIAETSSAADDVEFGAVPEEF
HHHHHHHCCCHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA