Click here to switch to the map view.

The map label for this gene is mdh [H]

Identifier: 227080643

GI number: 227080643

Start: 446844

End: 447905

Strand: Direct

Name: mdh [H]

Synonym: VCM66_0417

Alternate gene names: 227080643

Gene position: 446844-447905 (Clockwise)

Preceding gene: 227080638

Following gene: 227080644

Centisome position: 15.45

GC content: 49.44

Gene sequence:

>1062_bases
TTGCGCAAAGTCGCAAAGCTGACGTTAATTGATTGTAATCAGATTGTGATTACTATAGCGTTTGACGGTCGGATACACCT
AATCACCCTACAAACATATAAGAGATTACTCTCAAGGAGATTTACGATGAAAGTCGCTGTTATTGGTGCCGCTGGCGGCA
TTGGTCAAGCCCTTGCTCTGTTACTCAAAAACCGTCTTCCTGCTGGTTCTGATCTCGCGCTGTATGATATTGCGCCTGTC
ACACCGGGTGTCGCTGCGGATCTGAGTCATATCCCAACACCAGTTACCATCAAAGGCTATGCGGGTGAAGACCCAACGCC
TGCGCTGGAAGGTGCGGATGTGGTTCTGGTTTCTGCGGGTGTTGCACGTAAGCCGGGTATGGATCGTGCGGATCTGTTCA
ATGTGAACGCTGGCATTGTGAAAGCATTGGCAGAAAAAATTGCTGTGGTGTGTCCGAAGGCCTGTGTTGGCATTATTACT
AACCCAGTGAACACAACGGTCCCTATCGCAGCCGAAGTGCTGAAAAAAGCCGGTGTGTATGACAAGCGTAAGCTATTTGG
TGTCACCACACTGGATGTGATCCGCTCTGAAACCTTCGTTGCCGCGCTGAAAGATAAAGATCCGGGCCAAGTTCGTGTGC
CTGTGATTGGTGGTCACTCAGGGGTTACTATTCTGCCTCTGTTGTCGCAAGTAGAAGGCGTAAGCTTTACCGATGAAGAA
GTTGCCGCTTTGACTAAACGTATCCAAAACGCGGGTACCGAGGTGGTTGAAGCTAAGGCGGGTGGCGGTTCAGCGACTCT
TTCTATGGGCCAAGCGGCATGTCGTTTTGGTCTTGCACTGGTTAAAGCACTGCAAGGCGAATCTGATGTTGTTGAGTACG
CGTATGTAGAAGGTGAGGGGGAGTACGCTCCATTCTTTGCCCAACCTATCAAGCTAGGTAAAAATGGCGTTGAAGCGTTG
CTGGATATCGGCAAACTGAGCGCTTATGAGCAAGCCGCGCTGGATGGCATGTTAGATACACTCAAAGGCGACATTCAAAT
CGGTGTTGAGTTTGTAAAATAA

Upstream 100 bases:

>100_bases
AAATGTTTTTGTTATCAGGTTAGATCGCTGTATAACTCTTTGTCTCAATTGAACATTAACCGATAATCGTCTTTGATGCT
GTAACGCCTTCTCAGCAGTA

Downstream 100 bases:

>100_bases
TCGACAATCGATTCAAGCATTGGAAAATACCGCTCTTGAAGAGCGGTATTTTTTTATCTCAATGATATCCCCTTCCTACT
TGAAGCTGCAGCGGTGTTGA

Product: malate dehydrogenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 353; Mature: 353

Protein sequence:

>353_residues
MRKVAKLTLIDCNQIVITIAFDGRIHLITLQTYKRLLSRRFTMKVAVIGAAGGIGQALALLLKNRLPAGSDLALYDIAPV
TPGVAADLSHIPTPVTIKGYAGEDPTPALEGADVVLVSAGVARKPGMDRADLFNVNAGIVKALAEKIAVVCPKACVGIIT
NPVNTTVPIAAEVLKKAGVYDKRKLFGVTTLDVIRSETFVAALKDKDPGQVRVPVIGGHSGVTILPLLSQVEGVSFTDEE
VAALTKRIQNAGTEVVEAKAGGGSATLSMGQAACRFGLALVKALQGESDVVEYAYVEGEGEYAPFFAQPIKLGKNGVEAL
LDIGKLSAYEQAALDGMLDTLKGDIQIGVEFVK

Sequences:

>Translated_353_residues
MRKVAKLTLIDCNQIVITIAFDGRIHLITLQTYKRLLSRRFTMKVAVIGAAGGIGQALALLLKNRLPAGSDLALYDIAPV
TPGVAADLSHIPTPVTIKGYAGEDPTPALEGADVVLVSAGVARKPGMDRADLFNVNAGIVKALAEKIAVVCPKACVGIIT
NPVNTTVPIAAEVLKKAGVYDKRKLFGVTTLDVIRSETFVAALKDKDPGQVRVPVIGGHSGVTILPLLSQVEGVSFTDEE
VAALTKRIQNAGTEVVEAKAGGGSATLSMGQAACRFGLALVKALQGESDVVEYAYVEGEGEYAPFFAQPIKLGKNGVEAL
LDIGKLSAYEQAALDGMLDTLKGDIQIGVEFVK
>Mature_353_residues
MRKVAKLTLIDCNQIVITIAFDGRIHLITLQTYKRLLSRRFTMKVAVIGAAGGIGQALALLLKNRLPAGSDLALYDIAPV
TPGVAADLSHIPTPVTIKGYAGEDPTPALEGADVVLVSAGVARKPGMDRADLFNVNAGIVKALAEKIAVVCPKACVGIIT
NPVNTTVPIAAEVLKKAGVYDKRKLFGVTTLDVIRSETFVAALKDKDPGQVRVPVIGGHSGVTILPLLSQVEGVSFTDEE
VAALTKRIQNAGTEVVEAKAGGGSATLSMGQAACRFGLALVKALQGESDVVEYAYVEGEGEYAPFFAQPIKLGKNGVEAL
LDIGKLSAYEQAALDGMLDTLKGDIQIGVEFVK

Specific function: Catalyzes the reversible oxidation of malate to oxaloacetate [H]

COG id: COG0039

COG function: function code C; Malate/lactate dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the LDH/MDH superfamily. MDH type 1 family [H]

Homologues:

Organism=Homo sapiens, GI21735621, Length=325, Percent_Identity=54.1538461538462, Blast_Score=322, Evalue=4e-88,
Organism=Escherichia coli, GI1789632, Length=311, Percent_Identity=80.7073954983923, Blast_Score=501, Evalue=1e-143,
Organism=Caenorhabditis elegans, GI17554310, Length=325, Percent_Identity=55.3846153846154, Blast_Score=324, Evalue=5e-89,
Organism=Saccharomyces cerevisiae, GI6322765, Length=330, Percent_Identity=48.4848484848485, Blast_Score=277, Evalue=2e-75,
Organism=Saccharomyces cerevisiae, GI6320125, Length=339, Percent_Identity=38.6430678466077, Blast_Score=193, Evalue=3e-50,
Organism=Saccharomyces cerevisiae, GI6324446, Length=366, Percent_Identity=37.1584699453552, Blast_Score=191, Evalue=2e-49,
Organism=Drosophila melanogaster, GI24647881, Length=315, Percent_Identity=56.1904761904762, Blast_Score=323, Evalue=1e-88,
Organism=Drosophila melanogaster, GI24663599, Length=313, Percent_Identity=50.1597444089457, Blast_Score=273, Evalue=2e-73,
Organism=Drosophila melanogaster, GI24663595, Length=314, Percent_Identity=46.8152866242038, Blast_Score=250, Evalue=1e-66,

Paralogues:

None

Copy number: 2640 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2380 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 260 Molecules/Cell In: Stationary Phase

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001557
- InterPro:   IPR022383
- InterPro:   IPR001236
- InterPro:   IPR015955
- InterPro:   IPR001252
- InterPro:   IPR010097
- InterPro:   IPR016040 [H]

Pfam domain/function: PF02866 Ldh_1_C; PF00056 Ldh_1_N [H]

EC number: =1.1.1.37 [H]

Molecular weight: Translated: 36959; Mature: 36959

Theoretical pI: Translated: 7.38; Mature: 7.38

Prosite motif: PS00068 MDH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKVAKLTLIDCNQIVITIAFDGRIHLITLQTYKRLLSRRFTMKVAVIGAAGGIGQALAL
CCCCEEEEEEECCEEEEEEEECCEEEEEEHHHHHHHHHHHHEEEEEEEECCCCHHHHHHH
LLKNRLPAGSDLALYDIAPVTPGVAADLSHIPTPVTIKGYAGEDPTPALEGADVVLVSAG
HHHHCCCCCCCEEEEEECCCCCCCHHHHHHCCCCEEEEECCCCCCCCCCCCCCEEEEECC
VARKPGMDRADLFNVNAGIVKALAEKIAVVCPKACVGIITNPVNTTVPIAAEVLKKAGVY
CCCCCCCCHHHEEECCHHHHHHHHHHHHHHCCHHHHHHCCCCCCCCHHHHHHHHHHCCCC
DKRKLFGVTTLDVIRSETFVAALKDKDPGQVRVPVIGGHSGVTILPLLSQVEGVSFTDEE
CCHHHCCCHHHHHHHCCCEEEEECCCCCCEEEEEEEECCCCCCHHHHHHHHCCCCCCHHH
VAALTKRIQNAGTEVVEAKAGGGSATLSMGQAACRFGLALVKALQGESDVVEYAYVEGEG
HHHHHHHHHHCCCEEEEECCCCCCEEHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCC
EYAPFFAQPIKLGKNGVEALLDIGKLSAYEQAALDGMLDTLKGDIQIGVEFVK
CCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECC
>Mature Secondary Structure
MRKVAKLTLIDCNQIVITIAFDGRIHLITLQTYKRLLSRRFTMKVAVIGAAGGIGQALAL
CCCCEEEEEEECCEEEEEEEECCEEEEEEHHHHHHHHHHHHEEEEEEEECCCCHHHHHHH
LLKNRLPAGSDLALYDIAPVTPGVAADLSHIPTPVTIKGYAGEDPTPALEGADVVLVSAG
HHHHCCCCCCCEEEEEECCCCCCCHHHHHHCCCCEEEEECCCCCCCCCCCCCCEEEEECC
VARKPGMDRADLFNVNAGIVKALAEKIAVVCPKACVGIITNPVNTTVPIAAEVLKKAGVY
CCCCCCCCHHHEEECCHHHHHHHHHHHHHHCCHHHHHHCCCCCCCCHHHHHHHHHHCCCC
DKRKLFGVTTLDVIRSETFVAALKDKDPGQVRVPVIGGHSGVTILPLLSQVEGVSFTDEE
CCHHHCCCHHHHHHHCCCEEEEECCCCCCEEEEEEEECCCCCCHHHHHHHHCCCCCCHHH
VAALTKRIQNAGTEVVEAKAGGGSATLSMGQAACRFGLALVKALQGESDVVEYAYVEGEG
HHHHHHHHHHCCCEEEEECCCCCCEEHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCC
EYAPFFAQPIKLGKNGVEALLDIGKLSAYEQAALDGMLDTLKGDIQIGVEFVK
CCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10024551; 10952301; 10986258 [H]