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The map label for this gene is rfbB [H]

Identifier: 226950131

GI number: 226950131

Start: 3083548

End: 3084612

Strand: Reverse

Name: rfbB [H]

Synonym: CLM_3085

Alternate gene names: 226950131

Gene position: 3084612-3083548 (Counterclockwise)

Preceding gene: 226950132

Following gene: 226950130

Centisome position: 74.23

GC content: 25.73

Gene sequence:

>1065_bases
ATGAAAATATACTTAGTAACAGGAGGAGCAGGGTTTATAGGTTCTAACTTTATAATCTATATGCTTAATAAATATAATGA
AGAGCAAATAAAAATAATAAATTTAGATAAATTAACTTATGCAGGTAGCTTAGAAAATCTTAAATGCGTAGAAAATAAAA
ATAATTATATGTTTATAAAAGGTGATATATCCGATAAAAACTTAGTAGATAATGTTTTTAAACAATATGATATAGATTAT
ATAATAAATTTTGCGGCGGAATCCCATGTGGATAGAAGTATACAGGAACCACAAATTTTTGTAGAAACTAATGTTTTAGG
TACAGTAAATTTATTAAATACAGCTAAAAAATATTGGATATTTAATGATGTATTTAGAGAAGGCAAAAAATATTTACAGG
TTTCAACAGATGAAGTATATGGTTCTTTGGGCAAAAGTGGATATTTCACAGAAGATACTCCATTAGATCCTCATAGTCCA
TATTCAGCTAGTAAGGCTTCTGCTGATTTAATAGTAAGATCTTATTATGATACTTATAAAATGCCTATAAATATAACAAG
ATGTTCAAATAATTATGGGCCATATCAATTTCCAGAAAAATTAATTCCTCTTATAATAAATAATTATTTAAACAACAAAC
CTATTCCGATATATGGAGATGGTAAAAATATAAGGGATTGGTTATATGTAGAGGAACATTGTAGAGCTATAGATATGATT
ATACAGAACGGAAGAATGGGGGAAATATACAATATAGGTGGATATAATGAAAGAGAAAATGTATACATAATAAAATATAT
AATAGAAGTTTTAAACAAGATAACAAGAAATAATAAGAGACCTAATAAACTAATAAAGTATGTAAAAGATAGAAAAGGAC
ATGATAAAAGATATGCCATAGATCCATCAAAAATAGAAAATAAAATTAAATGGGAATCTCAAATTTCATTTGAAGATGGA
ATTTTAAAAACAGTAAATTGGTATTTAAATAATAAGCAGTGGCTTAATAAAGTAACTTCGGAAGAGTACCAAAGATACTA
TGAGAATATGTACAAAGGTAAATAA

Upstream 100 bases:

>100_bases
TAAATAAGGGAACTCCTTTGGAGTGGGAAATAATTGATTAATATGAAGTAGTTAGACAAATTTAGATATATAAATTATTT
TAAGGAGAATATTTTAATAT

Downstream 100 bases:

>100_bases
AAAATTGTAGGTTGGTGAATTAATAATGAAAGGAATAATACTTGCAGGAGGAAGTGGTACTCGATTGTATCCTTGCACTA
AGGCTATAAGTAAACAAATA

Product: dTDP-glucose 4,6-dehydratase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 354; Mature: 354

Protein sequence:

>354_residues
MKIYLVTGGAGFIGSNFIIYMLNKYNEEQIKIINLDKLTYAGSLENLKCVENKNNYMFIKGDISDKNLVDNVFKQYDIDY
IINFAAESHVDRSIQEPQIFVETNVLGTVNLLNTAKKYWIFNDVFREGKKYLQVSTDEVYGSLGKSGYFTEDTPLDPHSP
YSASKASADLIVRSYYDTYKMPINITRCSNNYGPYQFPEKLIPLIINNYLNNKPIPIYGDGKNIRDWLYVEEHCRAIDMI
IQNGRMGEIYNIGGYNERENVYIIKYIIEVLNKITRNNKRPNKLIKYVKDRKGHDKRYAIDPSKIENKIKWESQISFEDG
ILKTVNWYLNNKQWLNKVTSEEYQRYYENMYKGK

Sequences:

>Translated_354_residues
MKIYLVTGGAGFIGSNFIIYMLNKYNEEQIKIINLDKLTYAGSLENLKCVENKNNYMFIKGDISDKNLVDNVFKQYDIDY
IINFAAESHVDRSIQEPQIFVETNVLGTVNLLNTAKKYWIFNDVFREGKKYLQVSTDEVYGSLGKSGYFTEDTPLDPHSP
YSASKASADLIVRSYYDTYKMPINITRCSNNYGPYQFPEKLIPLIINNYLNNKPIPIYGDGKNIRDWLYVEEHCRAIDMI
IQNGRMGEIYNIGGYNERENVYIIKYIIEVLNKITRNNKRPNKLIKYVKDRKGHDKRYAIDPSKIENKIKWESQISFEDG
ILKTVNWYLNNKQWLNKVTSEEYQRYYENMYKGK
>Mature_354_residues
MKIYLVTGGAGFIGSNFIIYMLNKYNEEQIKIINLDKLTYAGSLENLKCVENKNNYMFIKGDISDKNLVDNVFKQYDIDY
IINFAAESHVDRSIQEPQIFVETNVLGTVNLLNTAKKYWIFNDVFREGKKYLQVSTDEVYGSLGKSGYFTEDTPLDPHSP
YSASKASADLIVRSYYDTYKMPINITRCSNNYGPYQFPEKLIPLIINNYLNNKPIPIYGDGKNIRDWLYVEEHCRAIDMI
IQNGRMGEIYNIGGYNERENVYIIKYIIEVLNKITRNNKRPNKLIKYVKDRKGHDKRYAIDPSKIENKIKWESQISFEDG
ILKTVNWYLNNKQWLNKVTSEEYQRYYENMYKGK

Specific function: Catalyzes the dehydration of dTDP-D-glucose to form dTDP-6-deoxy-D-xylo-4-hexulose via a three-step process involving oxidation, dehydration and reduction [H]

COG id: COG1088

COG function: function code M; dTDP-D-glucose 4,6-dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family. dTDP-glucose dehydratase subfamily [H]

Homologues:

Organism=Homo sapiens, GI7657641, Length=328, Percent_Identity=40.5487804878049, Blast_Score=235, Evalue=5e-62,
Organism=Homo sapiens, GI42516563, Length=333, Percent_Identity=21.6216216216216, Blast_Score=83, Evalue=4e-16,
Organism=Homo sapiens, GI56237023, Length=339, Percent_Identity=24.4837758112094, Blast_Score=73, Evalue=3e-13,
Organism=Homo sapiens, GI56118217, Length=339, Percent_Identity=24.4837758112094, Blast_Score=73, Evalue=3e-13,
Organism=Homo sapiens, GI189083684, Length=339, Percent_Identity=24.4837758112094, Blast_Score=73, Evalue=3e-13,
Organism=Homo sapiens, GI4504031, Length=350, Percent_Identity=23.4285714285714, Blast_Score=73, Evalue=4e-13,
Organism=Escherichia coli, GI1788353, Length=364, Percent_Identity=47.8021978021978, Blast_Score=347, Evalue=9e-97,
Organism=Escherichia coli, GI48994969, Length=350, Percent_Identity=47.7142857142857, Blast_Score=343, Evalue=9e-96,
Organism=Escherichia coli, GI1786974, Length=353, Percent_Identity=24.0793201133144, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI1788366, Length=363, Percent_Identity=22.5895316804408, Blast_Score=67, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17568069, Length=329, Percent_Identity=37.0820668693009, Blast_Score=217, Evalue=9e-57,
Organism=Caenorhabditis elegans, GI115532424, Length=331, Percent_Identity=33.5347432024169, Blast_Score=172, Evalue=3e-43,
Organism=Caenorhabditis elegans, GI17539532, Length=333, Percent_Identity=24.3243243243243, Blast_Score=86, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI17507723, Length=259, Percent_Identity=25.0965250965251, Blast_Score=67, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI133901786, Length=259, Percent_Identity=23.9382239382239, Blast_Score=66, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17539422, Length=259, Percent_Identity=23.9382239382239, Blast_Score=66, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI133901788, Length=259, Percent_Identity=23.9382239382239, Blast_Score=66, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI133901790, Length=259, Percent_Identity=23.9382239382239, Blast_Score=66, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI17539424, Length=259, Percent_Identity=23.9382239382239, Blast_Score=65, Evalue=4e-11,
Organism=Saccharomyces cerevisiae, GI6319493, Length=355, Percent_Identity=25.9154929577465, Blast_Score=94, Evalue=4e-20,
Organism=Drosophila melanogaster, GI21356223, Length=335, Percent_Identity=24.4776119402985, Blast_Score=93, Evalue=3e-19,
Organism=Drosophila melanogaster, GI19923002, Length=362, Percent_Identity=26.5193370165746, Blast_Score=79, Evalue=4e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005888
- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =4.2.1.46 [H]

Molecular weight: Translated: 41564; Mature: 41564

Theoretical pI: Translated: 8.89; Mature: 8.89

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIYLVTGGAGFIGSNFIIYMLNKYNEEQIKIINLDKLTYAGSLENLKCVENKNNYMFIK
CEEEEEECCCCCCCCCEEEEEEECCCCCEEEEEEEECEEECCCCCCEEEEECCCCEEEEE
GDISDKNLVDNVFKQYDIDYIINFAAESHVDRSIQEPQIFVETNVLGTVNLLNTAKKYWI
ECCCCHHHHHHHHHHCCHHHEEEHHHHHHHHHCCCCCEEEEEECCCEEHHHHHHHHHHHH
FNDVFREGKKYLQVSTDEVYGSLGKSGYFTEDTPLDPHSPYSASKASADLIVRSYYDTYK
HHHHHHCCHHEEEEEHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCEE
MPINITRCSNNYGPYQFPEKLIPLIINNYLNNKPIPIYGDGKNIRDWLYVEEHCRAIDMI
CCEEEEEECCCCCCCCCHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHHHHH
IQNGRMGEIYNIGGYNERENVYIIKYIIEVLNKITRNNKRPNKLIKYVKDRKGHDKRYAI
HCCCCCCCEEECCCCCCCCCEEEHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCEEEE
DPSKIENKIKWESQISFEDGILKTVNWYLNNKQWLNKVTSEEYQRYYENMYKGK
CHHHHHHHCCCCEECCCCCCHHEEHHEEECCHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKIYLVTGGAGFIGSNFIIYMLNKYNEEQIKIINLDKLTYAGSLENLKCVENKNNYMFIK
CEEEEEECCCCCCCCCEEEEEEECCCCCEEEEEEEECEEECCCCCCEEEEECCCCEEEEE
GDISDKNLVDNVFKQYDIDYIINFAAESHVDRSIQEPQIFVETNVLGTVNLLNTAKKYWI
ECCCCHHHHHHHHHHCCHHHEEEHHHHHHHHHCCCCCEEEEEECCCEEHHHHHHHHHHHH
FNDVFREGKKYLQVSTDEVYGSLGKSGYFTEDTPLDPHSPYSASKASADLIVRSYYDTYK
HHHHHHCCHHEEEEEHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCEE
MPINITRCSNNYGPYQFPEKLIPLIINNYLNNKPIPIYGDGKNIRDWLYVEEHCRAIDMI
CCEEEEEECCCCCCCCCHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHHHHH
IQNGRMGEIYNIGGYNERENVYIIKYIIEVLNKITRNNKRPNKLIKYVKDRKGHDKRYAI
HCCCCCCCEEECCCCCCCCCEEEHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCEEEE
DPSKIENKIKWESQISFEDGILKTVNWYLNNKQWLNKVTSEEYQRYYENMYKGK
CHHHHHHHCCCCEECCCCCCHHEEHHEEECCHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8253667 [H]