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The map label for this gene is rfbA [H]

Identifier: 226950130

GI number: 226950130

Start: 3082665

End: 3083522

Strand: Reverse

Name: rfbA [H]

Synonym: CLM_3084

Alternate gene names: 226950130

Gene position: 3083522-3082665 (Counterclockwise)

Preceding gene: 226950131

Following gene: 226950129

Centisome position: 74.21

GC content: 30.89

Gene sequence:

>858_bases
ATGAAAGGAATAATACTTGCAGGAGGAAGTGGTACTCGATTGTATCCTTGCACTAAGGCTATAAGTAAACAAATAATACC
TGTATATGATAAACCTATGATATATTATCCACTTTCTGTACTTATGCTTGCAGGTATAAGAGAAGTTTTAATAATATCCA
CAGAAAGAGATATAAGTCTATTTAAAGAATTATTAGGCAATGGAAATCAACTTGGAATGAATTTTAGCTATAATATTCAA
AAAGAGCCTAGAGGTATAGGAGAAGCATTTATTATAGGAAGAGATTTCATAGATAATGATTCCTGCTCATTAATATTAGG
TGATAATATTTTTTATGGACAAGGCTTCACGCCAATATTAGAGAAAGCAGCAAGTATAAATACTGGAGCAGGTATATTTG
CTTATTATGTAAATAACCCTAAAGATTATGGTATAGTGGAGTTTGATAAGAACTTCAATGTAGTTTCTATAGAGGAAAAA
CCGAAAGAGCCAAGATCTAATTATGCTATACCAGGATTATATTTTTATGATAATGATGTAGTGAATATTGCTAAAGAAAT
AAGACCATCTCCTAGGGGGGAATTAGAGATAACGGATATAAATAAAGTTTATTTAAGAAATAAAAATTTAAAAGTACAGG
TTTTAAGTAGAGGTTTTGCATGGCTTGATACAGGTACTCATGACGCTCTGTTAGAAGCTAGCAACTTTGTAAGAACAGTT
CAAAAAAGGCAAGGGCTGTATGTAGCATGCATTGAAGAGATAGCTTATAGACGAGGTTTTATAAATAAAGATCAGTTGTA
TGAATTATCAAATAGTCTTTTGAAAACAGATTATGGCAGGTATTTAAGAACTATTTAA

Upstream 100 bases:

>100_bases
AATAAGCAGTGGCTTAATAAAGTAACTTCGGAAGAGTACCAAAGATACTATGAGAATATGTACAAAGGTAAATAAAAAAT
TGTAGGTTGGTGAATTAATA

Downstream 100 bases:

>100_bases
ATATTTAAAAGAAAAATATGGAGGAATAGTAATGAGCAGTCTATATAAATTTAAAGGTAGGTTAAAAGAAGAGTTCCCAT
CTCAGATAATAGTGGATGTT

Product: glucose-1-phosphate thymidylyltransferase

Products: NA

Alternate protein names: G1P-TT 1; dTDP-glucose pyrophosphorylase 1; dTDP-glucose synthase 1 [H]

Number of amino acids: Translated: 285; Mature: 285

Protein sequence:

>285_residues
MKGIILAGGSGTRLYPCTKAISKQIIPVYDKPMIYYPLSVLMLAGIREVLIISTERDISLFKELLGNGNQLGMNFSYNIQ
KEPRGIGEAFIIGRDFIDNDSCSLILGDNIFYGQGFTPILEKAASINTGAGIFAYYVNNPKDYGIVEFDKNFNVVSIEEK
PKEPRSNYAIPGLYFYDNDVVNIAKEIRPSPRGELEITDINKVYLRNKNLKVQVLSRGFAWLDTGTHDALLEASNFVRTV
QKRQGLYVACIEEIAYRRGFINKDQLYELSNSLLKTDYGRYLRTI

Sequences:

>Translated_285_residues
MKGIILAGGSGTRLYPCTKAISKQIIPVYDKPMIYYPLSVLMLAGIREVLIISTERDISLFKELLGNGNQLGMNFSYNIQ
KEPRGIGEAFIIGRDFIDNDSCSLILGDNIFYGQGFTPILEKAASINTGAGIFAYYVNNPKDYGIVEFDKNFNVVSIEEK
PKEPRSNYAIPGLYFYDNDVVNIAKEIRPSPRGELEITDINKVYLRNKNLKVQVLSRGFAWLDTGTHDALLEASNFVRTV
QKRQGLYVACIEEIAYRRGFINKDQLYELSNSLLKTDYGRYLRTI
>Mature_285_residues
MKGIILAGGSGTRLYPCTKAISKQIIPVYDKPMIYYPLSVLMLAGIREVLIISTERDISLFKELLGNGNQLGMNFSYNIQ
KEPRGIGEAFIIGRDFIDNDSCSLILGDNIFYGQGFTPILEKAASINTGAGIFAYYVNNPKDYGIVEFDKNFNVVSIEEK
PKEPRSNYAIPGLYFYDNDVVNIAKEIRPSPRGELEITDINKVYLRNKNLKVQVLSRGFAWLDTGTHDALLEASNFVRTV
QKRQGLYVACIEEIAYRRGFINKDQLYELSNSLLKTDYGRYLRTI

Specific function: Catalyzes the formation of dTDP-glucose, from dTTP and glucose 1-phosphate, as well as its pyrophosphorolysis [H]

COG id: COG1209

COG function: function code M; dTDP-glucose pyrophosphorylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glucose-1-phosphate thymidylyltransferase family [H]

Homologues:

Organism=Homo sapiens, GI11761621, Length=251, Percent_Identity=24.7011952191235, Blast_Score=73, Evalue=3e-13,
Organism=Homo sapiens, GI11761619, Length=251, Percent_Identity=24.7011952191235, Blast_Score=73, Evalue=4e-13,
Organism=Escherichia coli, GI1788351, Length=281, Percent_Identity=60.8540925266904, Blast_Score=382, Evalue=1e-107,
Organism=Escherichia coli, GI1790224, Length=282, Percent_Identity=58.5106382978723, Blast_Score=367, Evalue=1e-103,
Organism=Caenorhabditis elegans, GI133931050, Length=198, Percent_Identity=26.7676767676768, Blast_Score=83, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6320148, Length=252, Percent_Identity=26.1904761904762, Blast_Score=79, Evalue=8e-16,
Organism=Drosophila melanogaster, GI21355443, Length=253, Percent_Identity=24.901185770751, Blast_Score=80, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24644084, Length=253, Percent_Identity=24.901185770751, Blast_Score=80, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005907
- InterPro:   IPR005835 [H]

Pfam domain/function: PF00483 NTP_transferase [H]

EC number: =2.7.7.24 [H]

Molecular weight: Translated: 32217; Mature: 32217

Theoretical pI: Translated: 8.36; Mature: 8.36

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKGIILAGGSGTRLYPCTKAISKQIIPVYDKPMIYYPLSVLMLAGIREVLIISTERDISL
CCCEEEECCCCCEEEHHHHHHHCCCCCCCCCCEEEHHHHHHHHHCCCEEEEEECCCHHHH
FKELLGNGNQLGMNFSYNIQKEPRGIGEAFIIGRDFIDNDSCSLILGDNIFYGQGFTPIL
HHHHHCCCCEEECEEEEECCCCCCCCCCEEEEECCCCCCCCCEEEECCEEEECCCCHHHH
EKAASINTGAGIFAYYVNNPKDYGIVEFDKNFNVVSIEEKPKEPRSNYAIPGLYFYDNDV
HHHHCCCCCCEEEEEEECCCCCCCEEEECCCCCEEEECCCCCCCCCCCCCCEEEEECCHH
VNIAKEIRPSPRGELEITDINKVYLRNKNLKVQVLSRGFAWLDTGTHDALLEASNFVRTV
HHHHHHCCCCCCCCEEEEECEEEEEECCCCEEEEEECCCEEEECCCHHHHHHHHHHHHHH
QKRQGLYVACIEEIAYRRGFINKDQLYELSNSLLKTDYGRYLRTI
HHHCCEEEEHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKGIILAGGSGTRLYPCTKAISKQIIPVYDKPMIYYPLSVLMLAGIREVLIISTERDISL
CCCEEEECCCCCEEEHHHHHHHCCCCCCCCCCEEEHHHHHHHHHCCCEEEEEECCCHHHH
FKELLGNGNQLGMNFSYNIQKEPRGIGEAFIIGRDFIDNDSCSLILGDNIFYGQGFTPIL
HHHHHCCCCEEECEEEEECCCCCCCCCCEEEEECCCCCCCCCEEEECCEEEECCCCHHHH
EKAASINTGAGIFAYYVNNPKDYGIVEFDKNFNVVSIEEKPKEPRSNYAIPGLYFYDNDV
HHHHCCCCCCEEEEEEECCCCCCCEEEECCCCCEEEECCCCCCCCCCCCCCEEEEECCHH
VNIAKEIRPSPRGELEITDINKVYLRNKNLKVQVLSRGFAWLDTGTHDALLEASNFVRTV
HHHHHHCCCCCCCCEEEEECEEEEEECCCCEEEEEECCCEEEECCCHHHHHHHHHHHHHH
QKRQGLYVACIEEIAYRRGFINKDQLYELSNSLLKTDYGRYLRTI
HHHCCEEEEHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7517391; 9097040; 9278503; 7517390 [H]