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The map label for this gene is megL [H]

Identifier: 226947242

GI number: 226947242

Start: 30101

End: 31303

Strand: Direct

Name: megL [H]

Synonym: CLM_0033

Alternate gene names: 226947242

Gene position: 30101-31303 (Clockwise)

Preceding gene: 226947241

Following gene: 226947243

Centisome position: 0.72

GC content: 33.42

Gene sequence:

>1203_bases
ATGGAAAATATAAAGAAAATGGGATTTGCGACAAAGGCTATACATGGAGGGCACATTGGAGATAAACAGTTTGGTTCATT
GGTAACTCCAATATACCAAACATCTACATTTATATTTGATTCAGCAGAGCAAGGGGGTAGGCGATTTGCAGGAGAAGAAA
GTGGATATATATATTCAAGATTAGGAAATCCTACATCTACAGAAGTAGAGAACAAGTTAGCTTTGTTAGAATGTGGAGAG
GCAGCTGTTGTAGCTGCATCAGGTATGGGTGCTATAGCTGCATCTTTGTGGTCAGCTTTAAAATCAGGAGATCATGTAGT
TGCATCAGATACTTTATATGGATGCACATTTGCACTATTAAATCATGGATTAACAAGATATGGAGTAGAGGTCACATTTG
TAGACGTATCTAATTTAGATGAAGTAAGAAATGCATTGAAAGCAAATACTAAAGTAGTTTATTTAGAAACTCCAGCTAAT
CCAACATTAAAGGTTACTGATATTAAACAAATATCAAATATGGTTCATGAAAATAATAAAGAATGTCTTGTATTTGTTGA
TAATACATTTTGTACACCATACATACAAAGGCCATTACAATTAGGCGCTGATGTAGTTGTACATTCTGCTACTAAATATT
TAAATGGACATGGAGATGTTATAGCTGGGTTTGCAGTAGGAAAAGAGGAATTTATAAATCAAGTTAAATTATTTGGTATA
AAAGACATGACAGGATCAGTTATAGGACCTTTTGAGGCATTTTTAATAATAAGAGGAATGAAAACATTACAATTAAGGAT
GGAAAAACATTGTAAGAATGCTATGAAAGTAGCAAAGTTTTTAGAGTCCCATCCGGCAGTTAAAAAAGTTTATTATCCAG
GATTAGAAAGTTTTGAATACTATGAGCTTGCTAAAAAGCAAATGAGTTTGCCCGGTGCTATGATTTCCTTTGAACTAAAA
GGTGGAGTAGAAGAAGGTAAAGTAGTTATGAATAATGTTAAATTAGCTACTCTTGCAGTAAGTCTTGGGGATGCTGAAAC
ATTAATTCAACATCCAGCATCAATGACACATTCACCTTATACAGCAGAAGAAAGAAAAGAAGCTGGTATAAGTGATGGGC
TAGTAAGACTATCCATAGGACTTGAAGATGTGGATGATATAATTAGTGATTTAAAACAAGCATTAGATTTAATAGTTAAG
TAA

Upstream 100 bases:

>100_bases
TATATCTTCAGGTTAATATAGTTAATTTATATATTTAAGACTATTGGTATATTATTTGCTTTAATAAATTACATATATTT
ATTTGAAAGGGGATTAATAC

Downstream 100 bases:

>100_bases
CTATTTTATAAAAAATATAAATGTTGATTCTTTATGCAAGGAATCAACATTTGCTTTTATAAATAAAAAAAGGTATAAAT
AATATTATATAAATAAATAG

Product: methionine gamma-lyase

Products: NA

Alternate protein names: L-methioninase [H]

Number of amino acids: Translated: 400; Mature: 400

Protein sequence:

>400_residues
MENIKKMGFATKAIHGGHIGDKQFGSLVTPIYQTSTFIFDSAEQGGRRFAGEESGYIYSRLGNPTSTEVENKLALLECGE
AAVVAASGMGAIAASLWSALKSGDHVVASDTLYGCTFALLNHGLTRYGVEVTFVDVSNLDEVRNALKANTKVVYLETPAN
PTLKVTDIKQISNMVHENNKECLVFVDNTFCTPYIQRPLQLGADVVVHSATKYLNGHGDVIAGFAVGKEEFINQVKLFGI
KDMTGSVIGPFEAFLIIRGMKTLQLRMEKHCKNAMKVAKFLESHPAVKKVYYPGLESFEYYELAKKQMSLPGAMISFELK
GGVEEGKVVMNNVKLATLAVSLGDAETLIQHPASMTHSPYTAEERKEAGISDGLVRLSIGLEDVDDIISDLKQALDLIVK

Sequences:

>Translated_400_residues
MENIKKMGFATKAIHGGHIGDKQFGSLVTPIYQTSTFIFDSAEQGGRRFAGEESGYIYSRLGNPTSTEVENKLALLECGE
AAVVAASGMGAIAASLWSALKSGDHVVASDTLYGCTFALLNHGLTRYGVEVTFVDVSNLDEVRNALKANTKVVYLETPAN
PTLKVTDIKQISNMVHENNKECLVFVDNTFCTPYIQRPLQLGADVVVHSATKYLNGHGDVIAGFAVGKEEFINQVKLFGI
KDMTGSVIGPFEAFLIIRGMKTLQLRMEKHCKNAMKVAKFLESHPAVKKVYYPGLESFEYYELAKKQMSLPGAMISFELK
GGVEEGKVVMNNVKLATLAVSLGDAETLIQHPASMTHSPYTAEERKEAGISDGLVRLSIGLEDVDDIISDLKQALDLIVK
>Mature_400_residues
MENIKKMGFATKAIHGGHIGDKQFGSLVTPIYQTSTFIFDSAEQGGRRFAGEESGYIYSRLGNPTSTEVENKLALLECGE
AAVVAASGMGAIAASLWSALKSGDHVVASDTLYGCTFALLNHGLTRYGVEVTFVDVSNLDEVRNALKANTKVVYLETPAN
PTLKVTDIKQISNMVHENNKECLVFVDNTFCTPYIQRPLQLGADVVVHSATKYLNGHGDVIAGFAVGKEEFINQVKLFGI
KDMTGSVIGPFEAFLIIRGMKTLQLRMEKHCKNAMKVAKFLESHPAVKKVYYPGLESFEYYELAKKQMSLPGAMISFELK
GGVEEGKVVMNNVKLATLAVSLGDAETLIQHPASMTHSPYTAEERKEAGISDGLVRLSIGLEDVDDIISDLKQALDLIVK

Specific function: Methionine biosynthesis; second step. [C]

COG id: COG0626

COG function: function code E; Cystathionine beta-lyases/cystathionine gamma-synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=390, Percent_Identity=43.0769230769231, Blast_Score=286, Evalue=3e-77,
Organism=Homo sapiens, GI299473759, Length=390, Percent_Identity=41.2820512820513, Blast_Score=256, Evalue=2e-68,
Organism=Homo sapiens, GI25453487, Length=390, Percent_Identity=37.1794871794872, Blast_Score=220, Evalue=2e-57,
Organism=Escherichia coli, GI1790375, Length=383, Percent_Identity=40.4699738903394, Blast_Score=271, Evalue=5e-74,
Organism=Escherichia coli, GI1789383, Length=392, Percent_Identity=29.8469387755102, Blast_Score=182, Evalue=3e-47,
Organism=Caenorhabditis elegans, GI17538055, Length=399, Percent_Identity=42.8571428571429, Blast_Score=288, Evalue=2e-78,
Organism=Caenorhabditis elegans, GI17560056, Length=394, Percent_Identity=41.1167512690355, Blast_Score=281, Evalue=3e-76,
Organism=Caenorhabditis elegans, GI71980708, Length=374, Percent_Identity=39.572192513369, Blast_Score=265, Evalue=2e-71,
Organism=Saccharomyces cerevisiae, GI6319307, Length=392, Percent_Identity=40.0510204081633, Blast_Score=272, Evalue=8e-74,
Organism=Saccharomyces cerevisiae, GI6323333, Length=437, Percent_Identity=34.0961098398169, Blast_Score=233, Evalue=4e-62,
Organism=Saccharomyces cerevisiae, GI6321254, Length=414, Percent_Identity=33.5748792270531, Blast_Score=184, Evalue=2e-47,
Organism=Saccharomyces cerevisiae, GI6321170, Length=342, Percent_Identity=29.2397660818713, Blast_Score=152, Evalue=9e-38,
Organism=Saccharomyces cerevisiae, GI6321904, Length=403, Percent_Identity=29.5285359801489, Blast_Score=142, Evalue=7e-35,
Organism=Drosophila melanogaster, GI19922554, Length=400, Percent_Identity=42.75, Blast_Score=322, Evalue=2e-88,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR006237
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: =4.4.1.11 [H]

Molecular weight: Translated: 43524; Mature: 43524

Theoretical pI: Translated: 6.29; Mature: 6.29

Prosite motif: PS00868 CYS_MET_METAB_PP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MENIKKMGFATKAIHGGHIGDKQFGSLVTPIYQTSTFIFDSAEQGGRRFAGEESGYIYSR
CCCHHHHCCHHHHCCCCCCCCHHHHHHHHHHHHHHEEEEECHHHCCCCCCCCCCCEEEEE
LGNPTSTEVENKLALLECGEAAVVAASGMGAIAASLWSALKSGDHVVASDTLYGCTFALL
CCCCCCHHHHHCEEEEECCCEEEEEECCHHHHHHHHHHHHHCCCEEEECCHHHHHHHHHH
NHGLTRYGVEVTFVDVSNLDEVRNALKANTKVVYLETPANPTLKVTDIKQISNMVHENNK
HCCHHHHCCEEEEEECCCHHHHHHHHHCCCEEEEEECCCCCCEEEEHHHHHHHHHHCCCC
ECLVFVDNTFCTPYIQRPLQLGADVVVHSATKYLNGHGDVIAGFAVGKEEFINQVKLFGI
CEEEEECCCCCCHHHHHHHHCCCHHHHHHHHHHHCCCCCEEEEEECCHHHHHHHHHHEEE
KDMTGSVIGPFEAFLIIRGMKTLQLRMEKHCKNAMKVAKFLESHPAVKKVYYPGLESFEY
CCCCCCCCCHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHEEECCCCCCHHH
YELAKKQMSLPGAMISFELKGGVEEGKVVMNNVKLATLAVSLGDAETLIQHPASMTHSPY
HHHHHHHHCCCCEEEEEEECCCCCCCCEEECCEEEEEEEEECCCHHHHHHCCHHCCCCCC
TAEERKEAGISDGLVRLSIGLEDVDDIISDLKQALDLIVK
CHHHHHHCCCCCCEEEEEECHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MENIKKMGFATKAIHGGHIGDKQFGSLVTPIYQTSTFIFDSAEQGGRRFAGEESGYIYSR
CCCHHHHCCHHHHCCCCCCCCHHHHHHHHHHHHHHEEEEECHHHCCCCCCCCCCCEEEEE
LGNPTSTEVENKLALLECGEAAVVAASGMGAIAASLWSALKSGDHVVASDTLYGCTFALL
CCCCCCHHHHHCEEEEECCCEEEEEECCHHHHHHHHHHHHHCCCEEEECCHHHHHHHHHH
NHGLTRYGVEVTFVDVSNLDEVRNALKANTKVVYLETPANPTLKVTDIKQISNMVHENNK
HCCHHHHCCEEEEEECCCHHHHHHHHHCCCEEEEEECCCCCCEEEEHHHHHHHHHHCCCC
ECLVFVDNTFCTPYIQRPLQLGADVVVHSATKYLNGHGDVIAGFAVGKEEFINQVKLFGI
CEEEEECCCCCCHHHHHHHHCCCHHHHHHHHHHHCCCCCEEEEEECCHHHHHHHHHHEEE
KDMTGSVIGPFEAFLIIRGMKTLQLRMEKHCKNAMKVAKFLESHPAVKKVYYPGLESFEY
CCCCCCCCCHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHEEECCCCCCHHH
YELAKKQMSLPGAMISFELKGGVEEGKVVMNNVKLATLAVSLGDAETLIQHPASMTHSPY
HHHHHHHHCCCCEEEEEEECCCCCCCCEEECCEEEEEEEEECCCHHHHHHCCHHCCCCCC
TAEERKEAGISDGLVRLSIGLEDVDDIISDLKQALDLIVK
CHHHHHHCCCCCCEEEEEECHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8586629; 9190812; 3365412 [H]