Click here to switch to the map view.

The map label for this gene is ywkE [H]

Identifier: 226225090

GI number: 226225090

Start: 2591317

End: 2592168

Strand: Reverse

Name: ywkE [H]

Synonym: Lm4b_02511

Alternate gene names: 226225090

Gene position: 2592168-2591317 (Counterclockwise)

Preceding gene: 226225091

Following gene: 226225089

Centisome position: 89.0

GC content: 37.79

Gene sequence:

>852_bases
ATGACGCAAATTAGTCAGTTGCTAAAAAATGCTGAAGCCATCCTTCTTGAAAAAGGGCTAGACCAAAATGCAGCAGAAAT
TTTACTAGAAACCAGAATGGGACTTTCGCGTTCTGAGCTTTGGATGGAAATTAATCGCGAACTTGAACCGAATCATGAAA
AGCAATTCGAGAAAGATTTTGCTAGATACTTAGCTGGCGAGCCAGTGCAGTATATTTTAAAAACAGCACCTTTTTATGGC
TATGATTTTTTAGTGACAGAAGATGTTCTTATTCCGCGGCCTGAAACAGAAGAGTTAGTCGCTTGTGCGGAGGATTTTTT
GAAAAAACATTCAGTTAAAAACGTGCTAGATGTTTGTACTGGAAGCGGGATTATCGCGATTGCACTCAAAAAAGCATTTC
CTGATATTTCGATGACGGCTTCTGATATTTCTGCTCCAGCACTTGCCGTGGCGAAAAAGAATGCATTGTTATTAAATGCG
GATGTGCGTTTTGTCGAAACAGATTTACTTGAAGCATTTAAACAAAACGAAGAACGATTCGATATGATTGTCGCCAATCC
GCCCTATATTTCTGAAGCAGAAAAAGCAGAAATGTCGGATTATGTGCTAAAAAACGAACCTTCATTAGCATTATTTGCTG
AAAATGATGGACTCGCTATTTATGAACGATTTGTGGATAACTTACAATATGTCCTAAATTCCTCTTTTTGGGTAGGGGTG
GAGATAGGTTATACACAAGGCGAGCGTGTAAAACAACTATTCGAAAAAAGTTATCCACATTCCACAGTTATTATCCACAA
AGATATCAATTCAAAAGATCGCTACGTAACATGCTCTAATATATTGGTATAA

Upstream 100 bases:

>100_bases
TTAACTATTCAAAAGCTAGATCAAATTATGGAAGGTAAACTCGATGAAATCATTGATGCGCTTATCCTAGAAGATCAAAC
AAGTAAATTGGAGCATTTAA

Downstream 100 bases:

>100_bases
AGGCGTTTCTATAAAAGAAGCGTCTTTTTCTTTGGAAAGTTATCCACAAAACTGTGGATAAAATGTTGTGCAAAAAAATT
TTTTAATCAAATCTTAATAA

Product: protoporphyrinogen oxidase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 283; Mature: 282

Protein sequence:

>283_residues
MTQISQLLKNAEAILLEKGLDQNAAEILLETRMGLSRSELWMEINRELEPNHEKQFEKDFARYLAGEPVQYILKTAPFYG
YDFLVTEDVLIPRPETEELVACAEDFLKKHSVKNVLDVCTGSGIIAIALKKAFPDISMTASDISAPALAVAKKNALLLNA
DVRFVETDLLEAFKQNEERFDMIVANPPYISEAEKAEMSDYVLKNEPSLALFAENDGLAIYERFVDNLQYVLNSSFWVGV
EIGYTQGERVKQLFEKSYPHSTVIIHKDINSKDRYVTCSNILV

Sequences:

>Translated_283_residues
MTQISQLLKNAEAILLEKGLDQNAAEILLETRMGLSRSELWMEINRELEPNHEKQFEKDFARYLAGEPVQYILKTAPFYG
YDFLVTEDVLIPRPETEELVACAEDFLKKHSVKNVLDVCTGSGIIAIALKKAFPDISMTASDISAPALAVAKKNALLLNA
DVRFVETDLLEAFKQNEERFDMIVANPPYISEAEKAEMSDYVLKNEPSLALFAENDGLAIYERFVDNLQYVLNSSFWVGV
EIGYTQGERVKQLFEKSYPHSTVIIHKDINSKDRYVTCSNILV
>Mature_282_residues
TQISQLLKNAEAILLEKGLDQNAAEILLETRMGLSRSELWMEINRELEPNHEKQFEKDFARYLAGEPVQYILKTAPFYGY
DFLVTEDVLIPRPETEELVACAEDFLKKHSVKNVLDVCTGSGIIAIALKKAFPDISMTASDISAPALAVAKKNALLLNAD
VRFVETDLLEAFKQNEERFDMIVANPPYISEAEKAEMSDYVLKNEPSLALFAENDGLAIYERFVDNLQYVLNSSFWVGVE
IGYTQGERVKQLFEKSYPHSTVIIHKDINSKDRYVTCSNILV

Specific function: Probable protein methyltransferase. May methylate a Gln residue in target proteins [H]

COG id: COG2890

COG function: function code J; Methylase of polypeptide chain release factors

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hemK family [H]

Homologues:

Organism=Escherichia coli, GI1787463, Length=231, Percent_Identity=32.034632034632, Blast_Score=108, Evalue=4e-25,
Organism=Escherichia coli, GI87082085, Length=164, Percent_Identity=37.8048780487805, Blast_Score=100, Evalue=9e-23,
Organism=Saccharomyces cerevisiae, GI6324266, Length=158, Percent_Identity=34.1772151898734, Blast_Score=83, Evalue=4e-17,
Organism=Drosophila melanogaster, GI24582226, Length=245, Percent_Identity=27.3469387755102, Blast_Score=79, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002052
- InterPro:   IPR004556
- InterPro:   IPR019874
- InterPro:   IPR007848 [H]

Pfam domain/function: PF05175 MTS [H]

EC number: 2.1.1.-

Molecular weight: Translated: 32025; Mature: 31894

Theoretical pI: Translated: 4.48; Mature: 4.48

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQISQLLKNAEAILLEKGLDQNAAEILLETRMGLSRSELWMEINRELEPNHEKQFEKDF
CCHHHHHHHHHHHEEHHCCCCCHHHHHHHHHHCCCCHHHHHHHHCCCCCCCHHHHHHHHH
ARYLAGEPVQYILKTAPFYGYDFLVTEDVLIPRPETEELVACAEDFLKKHSVKNVLDVCT
HHHHCCCHHHHHHHCCCCCCEEEEEECCEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHC
GSGIIAIALKKAFPDISMTASDISAPALAVAKKNALLLNADVRFVETDLLEAFKQNEERF
CCCEEEEEEHHHCCCCCCCHHHCCCCHHHEECCCEEEEECCCHHHHHHHHHHHHCCCCCE
DMIVANPPYISEAEKAEMSDYVLKNEPSLALFAENDGLAIYERFVDNLQYVLNSSFWVGV
EEEEECCCCCCCHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHHHCCCEEEEE
EIGYTQGERVKQLFEKSYPHSTVIIHKDINSKDRYVTCSNILV
EECCCCCHHHHHHHHHCCCCCEEEEECCCCCCCCEEECCCCCC
>Mature Secondary Structure 
TQISQLLKNAEAILLEKGLDQNAAEILLETRMGLSRSELWMEINRELEPNHEKQFEKDF
CHHHHHHHHHHHEEHHCCCCCHHHHHHHHHHCCCCHHHHHHHHCCCCCCCHHHHHHHHH
ARYLAGEPVQYILKTAPFYGYDFLVTEDVLIPRPETEELVACAEDFLKKHSVKNVLDVCT
HHHHCCCHHHHHHHCCCCCCEEEEEECCEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHC
GSGIIAIALKKAFPDISMTASDISAPALAVAKKNALLLNADVRFVETDLLEAFKQNEERF
CCCEEEEEEHHHCCCCCCCHHHCCCCHHHEECCCEEEEECCCHHHHHHHHHHHHCCCCCE
DMIVANPPYISEAEKAEMSDYVLKNEPSLALFAENDGLAIYERFVDNLQYVLNSSFWVGV
EEEEECCCCCCCHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHHHCCCEEEEE
EIGYTQGERVKQLFEKSYPHSTVIIHKDINSKDRYVTCSNILV
EECCCCCHHHHHHHHHCCCCCEEEEECCCCCCCCEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9353933; 9384377 [H]