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The map label for this gene is prfA

Identifier: 226225091

GI number: 226225091

Start: 2592155

End: 2593231

Strand: Reverse

Name: prfA

Synonym: Lm4b_02512

Alternate gene names: 226225091

Gene position: 2593231-2592155 (Counterclockwise)

Preceding gene: 226225092

Following gene: 226225090

Centisome position: 89.03

GC content: 42.34

Gene sequence:

>1077_bases
ATGTATGATCGATTACAGGCGGTGGAAGACCGTTATGATGAACTAAATGAGTTATTAAGTGATCCAGATGTAGTATCGGA
TCCAAAACGACTACGCGACCTTTCCAAAGAACAATCCGGCATTACTGCGACAGTCGAAACGTACCGCGAATACAAAAATG
TAAACGAACAAATTAACGAAACAAAAGAACTCCTAGGAGAAAAACTAGACGATGAAATGCGCGAAATGGCCAAAGAAGAA
TTCGCGGAACTTCAAAAAGAAAAGGCAGACTTAGAAGAACGACTAAAACTATTACTCGTTCCAAAAGATCCTAATGATGA
CAAAAACGTTATTTTAGAAATCCGCGGAGCAGCTGGTGGAGATGAAGCAGCTTTATTTGCCGGCGATTTATTCCGTATGT
ACAGTAAATATGCGGAATCACGTGGCTGGAAAGTAGAAATCATGGATGCGAACCCGACCGGTATTGGTGGTTACAAAGAA
ATTATTGCGATGATGAACGGAAATGATGCCTTTTCCCGAATGAAATATGAGAACGGTGCGCACCGTGTTCAACGTGTACC
AGAAACAGAATCAGGTGGTCGAATCCATACATCAACAGCGACAGTAGCCATTTTGCCAGAAGCGGAAGAAGTGGAAATTG
AACTGCACGATAAAGATATCCGCACTGATACATTTGCGTCTACTGGTGCCGGCGGACAAAGTGTCAATACGACGATGTCA
GCTGTTCGCTTAACGCATATTCCAACTGGGATTGTCGTATCGATGCAAGATGAACGTTCCCAGCTGAAAAATAAAGACAA
AGCGATGAAAGTATTACGCGCACGTGTATATGATAAATTTGAACGTGAAGCTCGCGAAGAATATGATGCCAACCGTAAAT
CAGCTGTAGGTACGGGTGACCGCTCTGAACGGATCCGGACTTACAACTATCCGCAAAACCGCGTAACCGACCATCGTATC
GGCTTAACTATTCAAAAGCTAGATCAAATTATGGAAGGTAAACTCGATGAAATCATTGATGCGCTTATCCTAGAAGATCA
AACAAGTAAATTGGAGCATTTAAATGACGCAAATTAG

Upstream 100 bases:

>100_bases
GAACAAATTATGATTGGCGGAAATGACCACTATTACCCTGTGTGTCGCAAATGTCATGCCAATCCACCAATAAAATAATT
AAAAGAGGTGTTGAAATACA

Downstream 100 bases:

>100_bases
TCAGTTGCTAAAAAATGCTGAAGCCATCCTTCTTGAAAAAGGGCTAGACCAAAATGCAGCAGAAATTTTACTAGAAACCA
GAATGGGACTTTCGCGTTCT

Product: peptide chain release factor 1

Products: NA

Alternate protein names: RF-1 [H]

Number of amino acids: Translated: 358; Mature: 358

Protein sequence:

>358_residues
MYDRLQAVEDRYDELNELLSDPDVVSDPKRLRDLSKEQSGITATVETYREYKNVNEQINETKELLGEKLDDEMREMAKEE
FAELQKEKADLEERLKLLLVPKDPNDDKNVILEIRGAAGGDEAALFAGDLFRMYSKYAESRGWKVEIMDANPTGIGGYKE
IIAMMNGNDAFSRMKYENGAHRVQRVPETESGGRIHTSTATVAILPEAEEVEIELHDKDIRTDTFASTGAGGQSVNTTMS
AVRLTHIPTGIVVSMQDERSQLKNKDKAMKVLRARVYDKFEREAREEYDANRKSAVGTGDRSERIRTYNYPQNRVTDHRI
GLTIQKLDQIMEGKLDEIIDALILEDQTSKLEHLNDAN

Sequences:

>Translated_358_residues
MYDRLQAVEDRYDELNELLSDPDVVSDPKRLRDLSKEQSGITATVETYREYKNVNEQINETKELLGEKLDDEMREMAKEE
FAELQKEKADLEERLKLLLVPKDPNDDKNVILEIRGAAGGDEAALFAGDLFRMYSKYAESRGWKVEIMDANPTGIGGYKE
IIAMMNGNDAFSRMKYENGAHRVQRVPETESGGRIHTSTATVAILPEAEEVEIELHDKDIRTDTFASTGAGGQSVNTTMS
AVRLTHIPTGIVVSMQDERSQLKNKDKAMKVLRARVYDKFEREAREEYDANRKSAVGTGDRSERIRTYNYPQNRVTDHRI
GLTIQKLDQIMEGKLDEIIDALILEDQTSKLEHLNDAN
>Mature_358_residues
MYDRLQAVEDRYDELNELLSDPDVVSDPKRLRDLSKEQSGITATVETYREYKNVNEQINETKELLGEKLDDEMREMAKEE
FAELQKEKADLEERLKLLLVPKDPNDDKNVILEIRGAAGGDEAALFAGDLFRMYSKYAESRGWKVEIMDANPTGIGGYKE
IIAMMNGNDAFSRMKYENGAHRVQRVPETESGGRIHTSTATVAILPEAEEVEIELHDKDIRTDTFASTGAGGQSVNTTMS
AVRLTHIPTGIVVSMQDERSQLKNKDKAMKVLRARVYDKFEREAREEYDANRKSAVGTGDRSERIRTYNYPQNRVTDHRI
GLTIQKLDQIMEGKLDEIIDALILEDQTSKLEHLNDAN

Specific function: Peptide chain release factor 1 directs the termination of translation in response to the peptide chain termination codons UAG and UAA [H]

COG id: COG0216

COG function: function code J; Protein chain release factor A

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family [H]

Homologues:

Organism=Homo sapiens, GI166795303, Length=322, Percent_Identity=44.0993788819876, Blast_Score=273, Evalue=3e-73,
Organism=Homo sapiens, GI34577120, Length=323, Percent_Identity=39.6284829721362, Blast_Score=234, Evalue=7e-62,
Organism=Homo sapiens, GI166795305, Length=227, Percent_Identity=41.8502202643172, Blast_Score=184, Evalue=9e-47,
Organism=Escherichia coli, GI1787462, Length=356, Percent_Identity=51.9662921348315, Blast_Score=358, Evalue=1e-100,
Organism=Escherichia coli, GI2367172, Length=338, Percent_Identity=36.9822485207101, Blast_Score=207, Evalue=6e-55,
Organism=Caenorhabditis elegans, GI17542784, Length=310, Percent_Identity=36.1290322580645, Blast_Score=176, Evalue=2e-44,
Organism=Saccharomyces cerevisiae, GI6321295, Length=334, Percent_Identity=43.1137724550898, Blast_Score=243, Evalue=4e-65,
Organism=Drosophila melanogaster, GI19921226, Length=325, Percent_Identity=40, Blast_Score=232, Evalue=3e-61,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR004373 [H]

Pfam domain/function: PF03462 PCRF; PF00472 RF-1 [H]

EC number: NA

Molecular weight: Translated: 40680; Mature: 40680

Theoretical pI: Translated: 4.64; Mature: 4.64

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYDRLQAVEDRYDELNELLSDPDVVSDPKRLRDLSKEQSGITATVETYREYKNVNEQINE
CCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
TKELLGEKLDDEMREMAKEEFAELQKEKADLEERLKLLLVPKDPNDDKNVILEIRGAAGG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHCEEEEECCCCCCCCEEEEEEECCCCC
DEAALFAGDLFRMYSKYAESRGWKVEIMDANPTGIGGYKEIIAMMNGNDAFSRMKYENGA
CCHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHCCCHHHHHHHHCCHH
HRVQRVPETESGGRIHTSTATVAILPEAEEVEIELHDKDIRTDTFASTGAGGQSVNTTMS
HHHHCCCCCCCCCEEEECCEEEEEECCCCEEEEEEECCCCCCHHCCCCCCCCCCHHHHHH
AVRLTHIPTGIVVSMQDERSQLKNKDKAMKVLRARVYDKFEREAREEYDANRKSAVGTGD
HHHHHCCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
RSERIRTYNYPQNRVTDHRIGLTIQKLDQIMEGKLDEIIDALILEDQTSKLEHLNDAN
CCCCCEECCCCCCCCCCHHHCCCHHHHHHHHHCCHHHHHHHHHHCCHHHHHHHCCCCC
>Mature Secondary Structure
MYDRLQAVEDRYDELNELLSDPDVVSDPKRLRDLSKEQSGITATVETYREYKNVNEQINE
CCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
TKELLGEKLDDEMREMAKEEFAELQKEKADLEERLKLLLVPKDPNDDKNVILEIRGAAGG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHCEEEEECCCCCCCCEEEEEEECCCCC
DEAALFAGDLFRMYSKYAESRGWKVEIMDANPTGIGGYKEIIAMMNGNDAFSRMKYENGA
CCHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHCCCHHHHHHHHCCHH
HRVQRVPETESGGRIHTSTATVAILPEAEEVEIELHDKDIRTDTFASTGAGGQSVNTTMS
HHHHCCCCCCCCCEEEECCEEEEEECCCCEEEEEEECCCCCCHHCCCCCCCCCCHHHHHH
AVRLTHIPTGIVVSMQDERSQLKNKDKAMKVLRARVYDKFEREAREEYDANRKSAVGTGD
HHHHHCCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
RSERIRTYNYPQNRVTDHRIGLTIQKLDQIMEGKLDEIIDALILEDQTSKLEHLNDAN
CCCCCEECCCCCCCCCCHHHCCCHHHHHHHHHCCHHHHHHHHHHCCHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11679669 [H]