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The map label for this gene is lytG [H]

Identifier: 226224811

GI number: 226224811

Start: 2297991

End: 2299094

Strand: Reverse

Name: lytG [H]

Synonym: Lm4b_02230

Alternate gene names: 226224811

Gene position: 2299094-2297991 (Counterclockwise)

Preceding gene: 226224813

Following gene: 226224810

Centisome position: 78.93

GC content: 36.14

Gene sequence:

>1104_bases
ATGAAAAAGAAATTCACGCAGATTTTACTAGCAAGCGTCATCGTAAGTTCTACACTAGTACCTTTTGTTCAAGCGGAGGC
AGCAGGAACGAGCCAAACTTCAGTAAGTCACGCAGGAATGACTTTATCACAACAACAATTTATACAATCCATAGCAAATG
ATGCACAAGATCTACAAAAAGAAGAAAAAATATTGACGAGTGTCACTTTAGCACAAGCTATTCTAGAATCCAATTGGGGT
AAAAGTGGCTTATCAACTTCAGCTAACAACCTGTTTGGAATTAAAGGAAGCTATGAAGGAAATTCCGTTTCAATGGGAAC
GCAAGAATTTAGCAGCGGGAAAGCCTACCGCACACAAGCTGATTTCAGAAAGTATCCAGACAAAAAAGCATCTTTAGTCG
ATCATGCCCAACTTTTTGTTAATGGAGTATCTGGTAATGCAAATCTTTACTCAGCGGTCATCGGAGAAACAAACTATAAG
GAAGCAGCATACGCAATTCAAGATGCAGGTTATGCAACTGACCCAGCATACGCAGAAAAACTCATTTCAACTATTGAAAA
TTACAATTTGGATCAATATGACCAAATTTACGATACAGTGACATCAACAGAAAACCAATTAGCATATGCGGAAATTAAAG
ATTCTAGCAATGAAACAATTTGGACAAAACCATATAATACAGAGGGTGCAGAAAAAACAGGTAGTGTCAAAGATTACAAA
AATAAAAGCCTTCGAATTTCTCAAAAAGCTGTCACAGAAAAAGGAACATGGTATCAGCTTCTAAATCAAGGCGAAACTAT
TGGATGGGTAAACAGTAATGCTGTAGAAGTTTTTTATACCCCGAAAAATGAAACAAATGTGAAACTAGATAAATATATCA
CCGATTCGGATCAAAAAATCTATGCTTACCCAGTAGAAGATAATTCAAAAGTAGTCACAAATCTTAATGATTATCTAGGG
AAAGAATTAGATATTGACCGTCGTGCAGATGTGAAAAATGAATATTGGTACCGTATTAAGTCAGACGATGGGAAAGTAAT
TGGCTGGTCAAAAGCAGACGGTTTTGCTGAAAACAATCCTAATAAAGCGTTAGAAGTAAAATAA

Upstream 100 bases:

>100_bases
GGCTCTCAGAATGAAAATGCTAGAATGAAGAAGGTTAACAGAGACAGAGCCATTTAAATTGGGGGAACTAACTAATTTGG
ATAGGAAGTGCCAACTGATA

Downstream 100 bases:

>100_bases
AAAAGTAGCAATCCAATTGCTTAATTGATAGTTCTCTGTTAAAATTAGCACCAGGTCATAAAAATAACCAGGAGAAAATT
TATTTTCACCCTGATGTAGT

Product: N-acetylmuramoyl-L-alanine amidase

Products: NA

Alternate protein names: Autolysin lytG; Exo-beta-N-acetylglucosaminidase lytG; Peptidoglycan hydrolase lytG [H]

Number of amino acids: Translated: 367; Mature: 367

Protein sequence:

>367_residues
MKKKFTQILLASVIVSSTLVPFVQAEAAGTSQTSVSHAGMTLSQQQFIQSIANDAQDLQKEEKILTSVTLAQAILESNWG
KSGLSTSANNLFGIKGSYEGNSVSMGTQEFSSGKAYRTQADFRKYPDKKASLVDHAQLFVNGVSGNANLYSAVIGETNYK
EAAYAIQDAGYATDPAYAEKLISTIENYNLDQYDQIYDTVTSTENQLAYAEIKDSSNETIWTKPYNTEGAEKTGSVKDYK
NKSLRISQKAVTEKGTWYQLLNQGETIGWVNSNAVEVFYTPKNETNVKLDKYITDSDQKIYAYPVEDNSKVVTNLNDYLG
KELDIDRRADVKNEYWYRIKSDDGKVIGWSKADGFAENNPNKALEVK

Sequences:

>Translated_367_residues
MKKKFTQILLASVIVSSTLVPFVQAEAAGTSQTSVSHAGMTLSQQQFIQSIANDAQDLQKEEKILTSVTLAQAILESNWG
KSGLSTSANNLFGIKGSYEGNSVSMGTQEFSSGKAYRTQADFRKYPDKKASLVDHAQLFVNGVSGNANLYSAVIGETNYK
EAAYAIQDAGYATDPAYAEKLISTIENYNLDQYDQIYDTVTSTENQLAYAEIKDSSNETIWTKPYNTEGAEKTGSVKDYK
NKSLRISQKAVTEKGTWYQLLNQGETIGWVNSNAVEVFYTPKNETNVKLDKYITDSDQKIYAYPVEDNSKVVTNLNDYLG
KELDIDRRADVKNEYWYRIKSDDGKVIGWSKADGFAENNPNKALEVK
>Mature_367_residues
MKKKFTQILLASVIVSSTLVPFVQAEAAGTSQTSVSHAGMTLSQQQFIQSIANDAQDLQKEEKILTSVTLAQAILESNWG
KSGLSTSANNLFGIKGSYEGNSVSMGTQEFSSGKAYRTQADFRKYPDKKASLVDHAQLFVNGVSGNANLYSAVIGETNYK
EAAYAIQDAGYATDPAYAEKLISTIENYNLDQYDQIYDTVTSTENQLAYAEIKDSSNETIWTKPYNTEGAEKTGSVKDYK
NKSLRISQKAVTEKGTWYQLLNQGETIGWVNSNAVEVFYTPKNETNVKLDKYITDSDQKIYAYPVEDNSKVVTNLNDYLG
KELDIDRRADVKNEYWYRIKSDDGKVIGWSKADGFAENNPNKALEVK

Specific function: Is the major glucosaminidase responsible for peptidoglycan structural determination during vegetative growth. Acts processively from the ends of the glycan strands. Also plays a role in motility, chemotaxis and cell division [H]

COG id: COG1705

COG function: function code NU; Muramidase (flagellum-specific)

Gene ontology:

Cell location: Secreted, cell wall [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 73 family [H]

Homologues:

Organism=Escherichia coli, GI1787321, Length=163, Percent_Identity=33.1288343558282, Blast_Score=80, Evalue=3e-16,

Paralogues:

None

Copy number: 10-20 (rich media) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000423
- InterPro:   IPR013338
- InterPro:   IPR002901 [H]

Pfam domain/function: PF01832 Glucosaminidase [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 40757; Mature: 40757

Theoretical pI: Translated: 4.85; Mature: 4.85

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
0.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
0.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKKFTQILLASVIVSSTLVPFVQAEAAGTSQTSVSHAGMTLSQQQFIQSIANDAQDLQK
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHCCCCCCHHHHHHHHHHHHHHHHH
EEKILTSVTLAQAILESNWGKSGLSTSANNLFGIKGSYEGNSVSMGTQEFSSGKAYRTQA
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEECCCCCCCCEECCCHHHCCCCCEECHH
DFRKYPDKKASLVDHAQLFVNGVSGNANLYSAVIGETNYKEAAYAIQDAGYATDPAYAEK
HHHHCCCHHHHHHHHEEHEEECCCCCCEEEEEECCCCCCHHHHHHHHCCCCCCCHHHHHH
LISTIENYNLDQYDQIYDTVTSTENQLAYAEIKDSSNETIWTKPYNTEGAEKTGSVKDYK
HHHHHHHCCCCHHHHHHHHHHCCCCCEEEEEECCCCCCEEEECCCCCCCCCCCCCCHHHC
NKSLRISQKAVTEKGTWYQLLNQGETIGWVNSNAVEVFYTPKNETNVKLDKYITDSDQKI
CCCEEEHHHHHHCCCHHHHHHHCCCEEEEECCCEEEEEECCCCCCCEEEEEEECCCCCEE
YAYPVEDNSKVVTNLNDYLGKELDIDRRADVKNEYWYRIKSDDGKVIGWSKADGFAENNP
EEEECCCCCEEEECCHHHCCCCCCCCCCCCCCCCEEEEEECCCCCEEEECCCCCCCCCCC
NKALEVK
CCEEECC
>Mature Secondary Structure
MKKKFTQILLASVIVSSTLVPFVQAEAAGTSQTSVSHAGMTLSQQQFIQSIANDAQDLQK
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHCCCCCCHHHHHHHHHHHHHHHHH
EEKILTSVTLAQAILESNWGKSGLSTSANNLFGIKGSYEGNSVSMGTQEFSSGKAYRTQA
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEECCCCCCCCEECCCHHHCCCCCEECHH
DFRKYPDKKASLVDHAQLFVNGVSGNANLYSAVIGETNYKEAAYAIQDAGYATDPAYAEK
HHHHCCCHHHHHHHHEEHEEECCCCCCEEEEEECCCCCCHHHHHHHHCCCCCCCHHHHHH
LISTIENYNLDQYDQIYDTVTSTENQLAYAEIKDSSNETIWTKPYNTEGAEKTGSVKDYK
HHHHHHHCCCCHHHHHHHHHHCCCCCEEEEEECCCCCCEEEECCCCCCCCCCCCCCHHHC
NKSLRISQKAVTEKGTWYQLLNQGETIGWVNSNAVEVFYTPKNETNVKLDKYITDSDQKI
CCCEEEHHHHHHCCCHHHHHHHCCCEEEEECCCEEEEEECCCCCCCEEEEEEECCCCCEE
YAYPVEDNSKVVTNLNDYLGKELDIDRRADVKNEYWYRIKSDDGKVIGWSKADGFAENNP
EEEECCCCCEEEECCHHHCCCCCCCCCCCCCCCCEEEEEECCCCCEEEECCCCCCCCCCC
NKALEVK
CCEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]